Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,813,2720CT26.5% 79.4 / 22.3 49S181L (TCG→TTG) dfpfused 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN‑binding
Reads supporting (aligned to +/- strand):  ref base C (1/35);  new base T (13/0);  total (14/35)
Fisher's exact test for biased strand distribution p-value = 5.33e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCG  >  NC_000913/3813054‑3813492
                                                                                                                                                                                                                             |                                                                                                                                                                                                                             
gcgggttgttCTGGTTCCCCCGGCCCCTGTGCCGGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCaa                                                                                                                                                                                                                      <  1:1686019/219‑1 (MQ=255)
       ggtgtGGCTACACCGCGGCCGGGGGCCGTGCCCGCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGa                                                                                                                                                                                                              <  1:1230332/225‑1 (MQ=255)
        gTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGGACGGCATTTTTTGCCGTCAACGAcccct                                                                                                                                                                                                              >  1:1513533/1‑225 (MQ=255)
               tACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGCGCCTTTTTTGCCCGTCCAACACCTGaaaaacatg                                                                                                                                                                                                      >  1:694632/1‑223 (MQ=255)
                            gtggcgtggCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCt                                                                                                                                                                                                       <  1:664462/207‑1 (MQ=255)
                            gTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGGGCGGCATTTTTTCCCCCCCACCACCCTCAAACATCTGaaatttttaca                                                                                                                                                                                          >  2:663294/1‑219 (MQ=255)
                                      cccccGCATTGAACCAGCAGATGTGCGGTGCCGCTGCGGCGCAGCATAATTTGGGGGTGTTTGCTGCCGGTGGTTTGCTCATCGGGGGGGCAGACGGTGGCAGCCGGGCTGGGGGTGATATGGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTGGCGGATTTTTCGCCCGTCAACGCCCTGAAACATCGGAAAATTATGATTACCGCCGGccc                                                                                                                                                                              <  2:980820/228‑1 (MQ=255)
                                       ccccccCATGACCCAGCGGTTGGCCCTGTCCGCTGCCACGAAGAATAATTAAGAGGTGCTTGCTTCCGGTGTTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGTTCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGCCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGccc                                                                                                                                                                              <  2:693412/228‑1 (MQ=255)
                                          cGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGAGCTTCTTTTGCCCCCTCACGACCCTAAACATCTGTAACTTTTTGTTTACCCCCGcccccc                                                                                                                                                                            >  2:460891/1‑226 (MQ=255)
                                                                  tGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACAt                                                                                                                                                                                                         <  1:1634105/175‑1 (MQ=255)
                                                                     cgttGCCCCGCGGCATAATTTAGGGGTGTTGGTTTCCCGGGGTTGCCTCATCGGGGGGCCAGAAGGGGGCATTAGGGCTTGGGGTGATTTCGGTCCGGGGCGAATGCTCGTTCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTta                                                                                                                                                <  2:1604228/225‑1 (MQ=255)
                                                                                tgcaTAATTTAGAGGTGTTTGCTTCCCGTGGTTGTCTCACTGGGGGCCCGAACGGGGGCCTTAGGTCTTGGGGTAATACCGGTCCTGGCGGAATGCTCGAACCGTTAACCATTGTGAATATGGCGGTAGCGCATTTTCCGCCCGTCACCGACCTGAAACATCTGAACATTATGATTCCCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATca                                                                                                                                    <  2:1510919/228‑1 (MQ=255)
                                                                                       tttAGGGGTGCTTGCTTCCCGTGGTTTGCCCATCTGGGGGCCAGACAGTGGCAGTCGGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTcc                                                                                                                             <  1:461704/229‑1 (MQ=255)
                                                                                            agGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAAc                                                                                                                                                                                                  <  1:472381/155‑1 (MQ=255)
                                                                                             ggTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGGGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCgg                                                                                                                           <  1:1232320/225‑1 (MQ=255)
                                                                                                 cttgcttCCTGGGGTTTGCTCATCGGGGGGCCAGACAGTGGCAGTCAGGCTTGGGGTGATATCGGTCCTGGGCGAAGGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGccc                                                                                                                                                                              <  2:1455268/170‑1 (MQ=255)
                                                                                                 cttgcttCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCCACCACCTGAAACATCTGAAAATTATGATTAACGCCGGccc                                                                                                                                                                              >  1:1457766/1‑170 (MQ=255)
                                                                                                 cttgcttCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCaa                                                                                                                                                                                                                      <  1:251953/131‑1 (MQ=255)
                                                                                                                        cTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGGGCCTTTTTTTCCCGTCAACGCCCCTAAACATCTGAACAATATGATTAACGCCCGCCCCACGCGGTAACCGCCCCCACCGCTGCGTTTTATTTCTCCTCACAGCCCCCGCCAGATGGGGTTTGGTATtgccgccgccgac                                                                                            >  2:804618/1‑227 (MQ=255)
                                                                                                                             ggCCAGACAGTGGCAGCTAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGGGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGc                                                                                                                                                                <  1:990464/156‑1 (MQ=255)
                                                                                                                                cAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGGGCCATTTTTTCCCCCCCACAACCCGGAACAACCTTACAATATGATTACCTCCCGCCCCCCGCCGCGACCGCCCGATCCCGTGCGGGATTTctct                                                                                                                                         >  2:1327988/1‑176 (MQ=255)
                                                                                                                                cAGACAGTGGAAGTCAGGCTTGTGGTGTTATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGCCCTGAAACATCTGAACATTATGTTTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATctct                                                                                                                                         <  1:1330284/176‑1 (MQ=255)
                                                                                                                                  gACAGTGGCAGGAAGGCTTGTGGTGATTTCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATAGGGCGGTGGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATc                                                                                                                                                          <  1:1342065/157‑1 (MQ=255)
                                                                                                                                          gCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGAATTTTTCGCCCGTCAACGACCTGAAACATCTGa                                                                                                                                                                                                    <  1:467501/108‑1 (MQ=255)
                                                                                                                                             gTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGa                                                                                                                                                                                                              <  1:213782/96‑1 (MQ=255)
                                                                                                                                                aGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGa                                                                                                                                                                                                              <  1:1737678/93‑1 (MQ=255)
                                                                                                                                                         tgatgtgcGGTCCGGGGCGAATGCTCGACCCTTTAACCTGTGTGGATATGGCGGTAGCGCTTTTTCCGCCCGTAAACGACCTGAACCATCTGAACATTATGATTCCCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACg                                                                    <  2:893102/214‑1 (MQ=255)
                                                                                                                                                            atatCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTTGGGCCCTTTTTTCCCGTCCACCACCTTGAACATCTGAACATTTTTATTTCCCCCCCCCCCACGCGTGAACCGcc                                                                                                                                                               >  2:163802/1‑126 (MQ=255)
                                                                                                                                                            atacCGGTCCTGGGCGAATGCTCGACCCGTTAACCATGGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGCCCTGAAACATCTGAACATTATGATGACCGCCGGCCCGACGCGTGAACCGCt                                                                                                                                                               <  1:164079/127‑1 (MQ=255)
                                                                                                                                                                 ggTCCTGGGCGAATGCTCGTTCCGTTACCCATTGTGGATTTGGCGGTAGCGCATTTTCCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGgcgc                                                                              <  1:44704/203‑1 (MQ=255)
                                                                                                                                                                 ggTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGCGCGTTTTTTTCCCGCCAACCACCTGGAAAACCTGAACAATATGATTAACCCCGGCCCCACCCGTGAACCGCCCCATCCCGGGCGGTTTATCTCTTATCACAACTCCCGCAAGAAGGGTGTTGCTTTCGTCGCCGCCCCGGCCCCTCGCGgggc                                                                              >  2:44634/1‑203 (MQ=255)
                                                                                                                                                                  gTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATTTGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCg                                                                                                                            <  1:1203221/156‑1 (MQ=255)
                                                                                                                                                                   tCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGGGCGATTTTTTCCCCTCAACGACCCTAAAAATc                                                                                                                                                                                                        >  2:747245/1‑80 (MQ=255)
                                                                                                                                                                   tCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATc                                                                                                                                                                                                        <  1:748554/80‑1 (MQ=255)
                                                                                                                                                                                          tAACCATTGTGGATATGGCGGTAGCGCATTTTTTGCCCGCCAACGACCTGAAACAACTGAAAATTATGATTACCGCCCGCCCGACCCGGGGACCGCCCCATCCgggg                                                                                                                                                     >  2:514033/1‑105 (MQ=255)
                                                                                                                                                                                          tAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTc                                                                                                                                                              <  1:282412/98‑1 (MQ=255)
                                                                                                                                                                                          tAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTg                                                                                                                                                     <  1:514942/107‑1 (MQ=255)
                                                                                                                                                                                               aTTGTGGATATGGCGGTAGCGCCTTTTTCGCCCCCCAAAAACCTGAAAAAATTGAACATTATGATTAACgcccgccc                                                                                                                                                                              >  1:822013/1‑77 (MQ=255)
                                                                                                                                                                                               aTTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGccc                                                                                                                                                                              <  2:820577/77‑1 (MQ=255)
                                                                                                                                                                                                 tgtgGATATGGCGGTAGCGCATTTTTTGCCCCGCCACGACCTGAAAAAt                                                                                                                                                                                                         >  1:64953/1‑49 (MQ=255)
                                                                                                                                                                                                 tgtgGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACAt                                                                                                                                                                                                         <  2:64845/49‑1 (MQ=255)
                                                                                                                                                                                                 tgtgGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTAAACCATCTGAACATTATGAt                                                                                                                                                                                          <  2:1167421/63‑1 (MQ=255)
                                                                                                                                                                                                  gtggtttgggCGGTGGCGCATTTTTCGCCCGGCAACGACCTGAACCATCTAAACATTATTATTCCCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACgtg                   <  1:1574634/221‑1 (MQ=255)
                                                                                                                                                                                                   tgGATATGGCGGTAGCGCATTTTTTGCCCGTCAACCACCTGAAACAACtt                                                                                                                                                                                                      >  1:1339029/1‑49 (MQ=255)
                                                                                                                                                                                                   tgGATATGGCGGTAGCGCATTGTTCGCCCGTCAACGACCTGAAACATCTg                                                                                                                                                                                                      <  2:1336717/50‑1 (MQ=255)
                                                                                                                                                                                                          ggCGGTGCCGCATTTTCCGCCCGCCACCGCCCTGAACCATCTGAACATTATGATTCCCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCg                                <  1:1051121/208‑1 (MQ=255)
                                                                                                                                                                                                          ggCGGTAGCTCATTTTTCGCCCGGCAACGCCCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGtt                 <  1:1505813/223‑1 (MQ=255)
                                                                                                                                                                                                                   cgcATTTTCCGCCCGTCAACGACCTGAACCATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACcgc   <  1:586219/229‑1 (MQ=255)
                                                                                                                                                                                                                     cattttttCGCCCGTCAACGCCCTGAACCATCTGAACATTGTGTTTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACcgcg  <  1:806020/226‑1 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                             
GACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCG  >  NC_000913/3813054‑3813492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: