Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6860GT46.4% 0.2 / 11.8 29intergenic (+40/+25)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (10/5);  new base T (9/4);  total (20/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTATCATCAT‑CCGTACGCTC‑GTTTACCGCC‑GGGCTGGACAAAAACT‑GCTTTAA‑CAA‑TTC‑AGGTAATAAATCGAGTTG‑TTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTC  >  NC_000913/2151511‑2151904
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aaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTatcatc                                                                                                                                                                     <  1:844606/239‑1 (MQ=255)
                                                                    tggATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCAACGAGCTAAGAGAAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAACTGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTATCATCAT‑CCGTACGCTC‑GTTTACCGCC‑GGGCTGGACAAAAACT‑GCTTTAA‑CAA‑TTc                                                                                                            <  1:1215913/220‑1 (MQ=255)
                                                                              aTTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACT‑CACTCTTCCCCCAGCGCCC‑GTGCCCACGTTTTATCATTAT‑CCGTTCGCTC‑GTTTACCGCC‑CGGCTGGGCAAAAACT‑GCTTTTA‑CCA‑TTT‑ATGTAATtaa                                                                                                 >  2:1215913/1‑222 (MQ=255)
                                                                                  gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGGA‑TTTTTGTTTTCAACCACCATGATAAAAA‑CACTCTGCCCCCAGCGCCA‑CGGCCAACGTTTTCTCAACAT‑ACCTACGCTCGGTTTCCCCCC‑CGCGTGGAC‑AAAACA‑ACTTTAACCAA‑CTC‑‑TGTACTAAATCTAGTTG‑TTctctccgtgtt                                                                           >  2:1941496/1‑229 (MQ=255)
                                                                                   tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGGTTTCTTCAAACGCCATCAGAAAAA‑CCATCCGCCCCCCACGGCA‑ATGCCAACGCTTTATTATCAT‑CCGTACGCTC‑GCTTACCGGC‑CGGCGGGACAAAAAAA‑CCTTTAA‑CCA‑TTA‑ACGTAATAACACTAgttg‑tttccctcgttcc                                                                           >  2:2224360/1‑227 (MQ=255)
                                                                                    ccccTATCAAAAAGAAGAAGCCGACGAGTAAAGAGAAAAATTAGTTCGTTAAT‑CAGCCGTTATTTTTTTTTTTTAAACGGGGGG‑TTGAT‑TTGTTGTCTTCAACCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGtt                                                                                                                                                                             <  1:171436/145‑1 (MQ=255)
                                                                                    cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTTTTTTCTTCAACCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCC‑GTGCCAACGTTTTTTCATCAT‑CCGTACGCCC‑GTTTACCCCC‑CGGCCGGACaaaaa                                                                                                                              >  1:635275/1‑191 (MQ=255)
                                                                                     aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAACCGCCCTGAGAAACT‑CACTCTGCCCCCAACGCCA‑GGGGCCACCTTTTTTTATTAT‑CCCGACGCCC‑GTTTTCCCCC‑GGGGCGGGCAAAAAAC‑GCCTTTA‑CAA‑ATCTAGGAAAAAAAACGAGTTG‑TT‑CTGCGGtgctgc                                                                         >  2:450245/1‑238 (MQ=255)
                                                                                       cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTTTTGTTTTCCAACCCCCTGAGAAAAT‑AACTCTGCCCCCAGCGCCC‑GGGCCAACGGTTTATCATCAT‑CCCTACGCTC‑CTTTTCCGCCGGGGCGGGGCAAAAA‑T‑GCTTTTA‑AAA‑TTC‑AGGTGATAAAATGAGTTGTTTCCTGcttgtgtccc                                                                        >  2:75961/1‑229 (MQ=255)
                                                                                       cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACT‑CACTCTGCCCCCAGCGCCA‑GTGCCCACGTTTTATCATCAT‑CCGTACGCTC‑GTTTACCGCC‑GGGCTGGACAAAAAAT‑GCTTTAA‑CAA‑TTT‑AGGAAATAAATCAAGTTG‑TTCCTGCGTTGCTgccg                                                                       >  2:1573630/1‑238 (MQ=255)
                                                                                             aaaaaaAGAGGCGGACGAGCAAAGAAAAAAATTATTTCCTTAAT‑CGCCTTTTATTTTTTTTTTTAAACCTGGGGT‑TTAAT‑TTTTTTTCTAAAACCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTATCATCAT‑CCGTa                                                                                                                                                             <  1:504397/153‑1 (MQ=255)
                                                                                              aaaGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTTTTTTTTTCCACCGCCCTGAGAAAAT‑CACTCTGCCCACAGCGCCA‑GTGCCCACGTTTTatcatcat                                                                                                                                                                   >  2:171436/1‑147 (MQ=255)
                                                                                                    gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTTTA‑TTTTGGTCTTCACACACACTAAAAAAAC‑CACCCTGCCCCCAGCCCCC‑AGGCCAACGTTTTATAATCAT‑CCGTCCGCCC‑GTTTAACGCC‑GCGCTGGACAAAAAATCTTTTTAA‑CAA‑‑TC‑AGGTATAAAAACGCATTT‑TTCCTGCCGTTCCGCCGCCactgcctttaaa                                                         >  2:657962/1‑228 (MQ=255)
                                                                                                    gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑ATTGA‑TTTTTTTCTTCAACCGCCCTGAAGAAAT‑CACTCTTCCCCCCGCGCCAGGTGCCAA‑GGTTTATTACTATACCGAACG‑AC‑GTTTTCCCCC‑CGGCCGGGCAAAAAAT‑CCCTTAA‑AAA‑ATC‑TGAGAATAAATCGAGGTG‑GTCCTTCGTTGCTGCCGCCCGttccttcata                                                         >  2:2151662/1‑230 (MQ=255)
                                                                                                       gCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGTTGTTTTCAACCCCCCTGAGAAACT‑CACTCTTCCCACAGCGCCA‑GGGCCCACGTTTTTTCATCAT‑CCCTACGCTC‑Ctttt                                                                                                                                                  >  2:504397/1‑152 (MQ=255)
                                                                                                        ccGACGACCTAAGAGAAAAATTAGTTCGCAAAT‑CGGCCTTTAT‑TTTTTTTTTTAAACCGGGGGTTTGAT‑TTTTTGTCTTAAACCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCaa                                                                                                                                                                                 <  1:1229340/123‑1 (MQ=255)
                                                                                                                  aaGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGGTTTGAA‑TT‑TTTTTTTCAACCGCCATGAGAAAAT‑CACCCTGCCCACAGCGCCC‑GTGCCAACGTTTTttca                                                                                                                                                                       >  2:1229340/1‑123 (MQ=255)
                                                                                                                   agagAAAAATTATTTCCCTTAC‑CAGCCGTTATATTTATTGTTTAAACTGGCGT‑TTGAT‑TTTTTGTCTTCACCCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTATCCTCAT‑CCGTCCGCTC‑GTTTACCGCC‑GGGCTGGACAAAAACT‑GCTTTAA‑CAA‑TTC‑AGGTAATAAATCGAGTTG‑TTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                          <  2:711978/239‑1 (MQ=255)
                                                                                                                        aaatTTAGTTCGCTAAC‑CAGCCGTTATATTTTTTTTTAAAACGGGGGG‑TTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACT‑CACTCTGCCCACAgcgc                                                                                                                                                                                           <  1:411099/98‑1 (MQ=255)
                                                                                                                         aaaTTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAAACGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTg                                                                                                                                                                                     >  1:1954762/1‑102 (MQ=255)
                                                                                                                          aaTTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTTAA‑TTTTTTTCTTTCACCCCCATTGGAAAAT‑CACTTTGCCCCCAGCGCCC‑GTGCCCAACTTTTTTCATCAT‑CCCTACGCCC‑GTTTACCCCC‑CGGCTGGACAAAAACT‑GCTTTTA‑CAA‑TTT‑AGGTAATAAATAGAGTTT‑TTTCTGCGTTTGTGCCGCCAGTCCATTGTAAAAAGGGGGAAAGAAAGGcacaa                                   >  2:510204/1‑238 (MQ=255)
                                                                                                                             tAGTTC‑CTAATCCAGCTTTTATATTTTTTG‑TTAAACCGGCGG‑GTGAATTTTTTGTTTCCAACCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTATCATCAT‑CCGTACGCTC‑GTTTACCGCC‑GGGCTGGACAAAAACT‑GCTTTAA‑CAA‑TTC‑AGGTAATAAATCGAGTTG‑TTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                <  1:872130/239‑1 (MQ=255)
                                                                                                                                  cGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTTAA‑TTTTTGTTTTTAACCGCCCTGAGAAACT‑CACTCTGCCCCCAGCGCCA‑GTGCCCAc                                                                                                                                                                                >  2:411099/1‑98 (MQ=255)
                                                                                                                                             cttttATATTTTTTTTTTAACGTGTGGT‑TTGAT‑TTGTTTTCTCAACCCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTATCATCAT‑CCGTACGCTC‑GTTTACCGCC‑GGGCTGGACAAAAACT‑GCTTTAA‑CAA‑TTC‑AGGTAATAAATCGAGTTG‑TTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                  <  1:2036083/235‑1 (MQ=255)
                                                                                                                                                        attGTTTAAACTGGCGG‑TTGAA‑TTTTTTCCTTTACCCTCCATTAAAAATTCCACT‑TGCCCCCCGCGCCT‑GCGCCCACGTTTTTTTACTAT‑CCGTTCGCCC‑TTTTACCCCC‑CGGCTGGACAAAAAAT‑GCCTTTA‑CAA‑TTT‑ATGCTATAAATCTAGGTG‑TTCCTCCGTTGCTGCCGCCAGTCCATTTTAAAAAGGGGGAAAGAAAAGCACAAGAGGAGAAATATCCAGCAAAATTAACgac      >  2:679316/1‑237 (MQ=255)
                                                                                                                                                             ttAAACTGGCGG‑TTTAA‑TTGTTTTCTTCAACCCCCCTGAGAAAAT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTATTATCAT‑CCGTACGCCC‑GTTTACCGCC‑GGGCTGGACAAAAACT‑GCTTTAA‑CAA‑TTC‑AGGTAATAAATCGAGTTG‑TTTCTGCGTTGCTGCCGCCAGTCCATTTAAAAAAGGGGGAAAAAAAAGCACATGAGGAGAATTATCCAACATAttttaacccaatc  >  2:1618838/1‑228 (MQ=255)
                                                                                                                                                             ttAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAAACGCCCTGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTATCATCAT‑CCGTACGCTC‑GTTTACCGCC‑GGGCTGGACaaaaa                                                                                                                              >  1:623349/1‑119 (MQ=255)
                                                                                                                                                              tAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAACCGCCCTGAGAAAAA‑CACTTTGCCCCCAGCGCCC‑GCGGCAACGCTTTTTTTTCCT‑CCGCTTGCCC‑GTTTACCGCC‑CGGCTGGGCAAAAAAT‑CCTTTTA‑CAATTTC‑GGGTAA‑AAATCGGGGTG‑TTTCTGCGTTTTTGCCCCCAGTTCATTGAAAAAAGGGGGAAAAAAAAGCCCATGAGGGGGATTATCCAGCATATTTTACg        >  2:370069/1‑231 (MQ=255)
                                                                                                                                                               aaaCTGGCGG‑TTGAA‑TTGTTTTCTTCCACCGCCCTGAGAAACT‑CACTCTGCCCCCAGCGCCA‑GTGCCCACGGTTTATCATCAT‑CCGTACGCTC‑GTTTACCCCC‑GGGCTGGACAAAAACT‑GCTTTAA‑CAA‑TTC‑AGGGAATAAATCGAGTTG‑TTCCCGCGTTGCTGCCGCCCGTCCATTGAAAAAAGGGGGAAAGAAAAGCACATGAGGAGGATTATTCAGCATATTTAACTCCAATc  >  2:1469485/1‑236 (MQ=255)
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AATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACT‑CACTCTGCCCACAGCGCCA‑GTGCCAACGTTTTATCATCAT‑CCGTACGCTC‑GTTTACCGCC‑GGGCTGGACAAAAACT‑GCTTTAA‑CAA‑TTC‑AGGTAATAAATCGAGTTG‑TTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTC  >  NC_000913/2151511‑2151904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑