Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,745,0030AG40.7% 82.1 / 17.4 59E67G (GAA→GGA) yiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
Reads supporting (aligned to +/- strand):  ref base A (8/27);  new base G (24/0);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 5.12e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAATTCTCTAACGTCGCCACCGCAAGGAGCTTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCATTGTT‑GACCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCT  >  NC_000913/3744766‑3745229
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         
aGCAATTCTCTAACGTCGCCACCGCAAGGAGCTTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGa                                                                                                                                                                                                                                           <  1:308444/238‑1 (MQ=255)
       ctctAACGTCGCCACCGCAAGGAGCTTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGa                                                                                                                                                                                                                                  <  1:2252654/239‑1 (MQ=255)
            aCGTCGCCACCGCAAGGAGCTTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑TCATGGAT‑tcg                                                                                                                                                                                                                            >  1:1139043/1‑239 (MQ=255)
               tCGCCACCGCAAGGAGCTTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑ACAttcatggtggg                                                                                                                                                                                                                          >  2:2031599/1‑229 (MQ=255)
                 gCCACCGCAAGGAGCTTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑TCATGAAT‑tcggggg                                                                                                                                                                                                                        >  1:396176/1‑236 (MQ=255)
                  ccACCGCAAGGAGATTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑TCATGAAT‑TCCgggggc                                                                                                                                                                                                                      >  1:787617/1‑239 (MQ=255)
                      cGCAAGGAGCTTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑ACATGAAT‑gggggggg                                                                                                                                                                                                                       >  1:1031667/1‑234 (MQ=255)
                         aaGGAGCTTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑a                                                                                                                                                                                                              <  1:859539/238‑1 (MQ=255)
                                     cATGGCGGTGCTGATTTTTCTGGGCTGTTGGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCGGGCAACTGTTGTTGGGCGGGGCGGCGTAAATGTTCTGACGGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGACGGGAGCGGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATAAAG‑GCGGGGGGCT‑TGTA‑AAAACGCAttgtt‑g                                                                                                                                                                                                <  2:1988278/239‑1 (MQ=255)
                                       tGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑TCATTAAA‑GCGGGCGGGT‑TTTC‑CAAAAGCCTTGTT‑Ga                                                                                                                                                                                               >  1:571517/1‑238 (MQ=255)
                                       tGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑gcggg                                                                                                                                                                                                                          <  1:613957/214‑1 (MQ=255)
                                       tGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGAAA‑TCATGAAT‑gcggg                                                                                                                                                                                                                          >  2:613957/1‑214 (MQ=255)
                                        gtctttgctattttgtCGGGGTTTTCTGTGGGGTGTTATGGCTGT‑GGTTTGCCCTATGGCCTGGGCCTTTTTGTTGGGGGGGGCGGCTTTAATGTTCTGCCTGAATATGTTTGATGTCCAG‑TTCAGGGCAAAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGGGGGGGAAA‑TCATGAAG‑GCGGGCGGCT‑TGTC‑AAACCGCTTGGTT‑GAc                                                                                                                                                                                              <  2:1537822/227‑1 (MQ=255)
                                         gCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑ATATGAAT‑GCGGGCGGGC‑TGTC‑AAAACGCACTGTT‑GAccc                                                                                                                                                                                            >  1:1442205/1‑238 (MQ=255)
                                         gCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑ACATGAAA‑TCGGGGGGCT‑TGTT‑AAAACGCCatgttta                                                                                                                                                                                                >  1:1150385/1‑234 (MQ=255)
                                             tgCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑TCATGACT‑GCTGGGGGCTGTGTC‑AAAA‑GCCttgtttgaccgcc                                                                                                                                                                                          >  2:373854/1‑230 (MQ=255)
                                             tgCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑ACATTAAT‑GAGGGCGGGC‑GGTT‑CAAACGCAttgtttgcgctgcc                                                                                                                                                                                         >  2:27500/1‑230 (MQ=255)
                                             tgCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑ACATGAAT‑GGGGGGGGCT‑TGTC‑AAAAACCATTTTT‑TACCTGCCg                                                                                                                                                                                        >  1:1272209/1‑239 (MQ=255)
                                             tgCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGAAA‑ACATGAAT‑GGGGGGGGGC‑TGTC‑TACACACCTTGTT‑GGCCGGcac                                                                                                                                                                                        >  2:1358660/1‑237 (MQ=255)
                                             tgCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCCATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑TTATGGCT‑GGCGGGGGCG‑TCTC‑CAAACCCAttgcgtt                                                                                                                                                                                                >  2:1699544/1‑228 (MQ=255)
                                                        cTGGGTTGTTTTTGGGGTGGTTTTGCTATGGTTGTG‑CTTATTGCCGGGGCAGTTTTGTTGGGCGGGGCGGCGTAAATGTTCTGGCGGGACTTTTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGACCGGGGCGAATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGGGGGTGAAA‑TCATGAAG‑GGGGGCGGCT‑TGTC‑AAAACGCATTGTT‑GACCTGCCGATGAAAC‑tggt                                                                                                                                                                            <  2:1812731/239‑1 (MQ=255)
                                                         tGGGTTGTCTGTGGGGTGGTTTTGTTATCGGTTGG‑CTTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGAATTGTTTGATGTCCAG‑ATCATGGCACAAGCGCTGGTGAAGGGGGCCGATGGCTTCTCCCGGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCAGGAAT‑GCGGGGGGTT‑TTTA‑AAACCGCATTGTT‑GACCTGCCGATGAAAC‑tggtg                                                                                                                                                                           <  2:1218543/239‑1 (MQ=255)
                                                         tGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑TCATGAAT‑GCGGGCGGCT‑TTTT‑AAAACGCATTGTT‑TACCCGGCGAGTAAAC‑tggtg                                                                                                                                                                           >  1:236177/1‑239 (MQ=255)
                                                                       ggtggtATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑TCATGTAG‑ACGGGCGGGC‑TGTCAAAAACGAATTG‑T‑GATGTGGCTGTGAAAA‑CGGTGGGGGATAAAACaag                                                                                                                                                             >  2:913842/1‑237 (MQ=255)
                                                                          ggtATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCATTGTT‑GACCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCgg                                                                                                                                                          >  1:1304870/1‑239 (MQ=255)
                                                                          ggtATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑TCATGAAT‑GCGGGCGGCC‑TGTC‑CAAAAGCATTGTT‑GACCTGCCGGTGAAAC‑TGGTGGGGGGTAAAACAGGCgg                                                                                                                                                          >  1:1425572/1‑239 (MQ=255)
                                                                          ggtATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑ACATGAAT‑GCGGGCGGCC‑TGTC‑AAAAAGCCTTGTT‑GACCTGCCGATGAAAC‑TGGGGGGGCATAAACCAGGCgg                                                                                                                                                          >  1:2023029/1‑239 (MQ=255)
                                                                          ggtATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCATTGTT‑TGACTGCCCATGAAAC‑TGGTGGGGGATAAACCAGGCgg                                                                                                                                                          >  1:1486618/1‑239 (MQ=255)
                                                                          ggtATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑ACCTGGATGGGGGGGGGGT‑TGTC‑AAACTCCATT‑TT‑GGTCTGCCGGTGGAAC‑ATGTGGGGGGTATAACAGGCgc                                                                                                                                                          >  2:1159249/1‑238 (MQ=255)
                                                                          ggtATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGCGGAA‑TCCTGAAT‑GCGGGCGGCT‑TGTC‑AAAAAGCCTTGTT‑GTCCTGCCGATGAAAA‑TGGTGGGGCATAAACCAGGCgg                                                                                                                                                          >  1:247038/1‑239 (MQ=255)
                                                                            tATTGCTATCGTTTGT‑CCTATTGCCGGGGCACTGTTGTTGGGGGGGGGGGCGTTAATGTTCTGGCTGGAAATGTTTGATGTCCAG‑ATCAGGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTTTTTTTGCTGGCGGGTAAAA‑TCATGAAT‑GCGGGCGGTT‑TGTA‑AAACCGC‑TTGTTGGCCCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCggg                                                                                                                                                         <  2:189801/238‑1 (MQ=255)
                                                                              ttGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑ACATTAAT‑TGGGGCGGCC‑TGTCGAAAACCATTTTTT‑GCCCTGCC‑ATGAAACATGGGGGGGGAAAAAcagggggg                                                                                                                                                          >  2:1237199/1‑229 (MQ=255)
                                                                                   ctggttttG‑CTTTTTGCCGGGGCACTGTTGTTGTGCGGGGCGGCTTTAATGTTCGGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGTGATTCCG‑TTTTTTGTGTTGGCGGGTGAAA‑TCATGAAT‑GCGGGGGGTT‑TGTC‑AAAACGCTTTGTT‑GACCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑a                                                                                                                                                <  2:1150385/234‑1 (MQ=255)
                                                                                   aTCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGGAA‑TCATGAAT‑GCGGGGGGCT‑TGTT‑CAAAAGCAGTTTT‑GACCTGCCGATGAAAC‑TGGGGGGGCATAAACCAAGCggggcggggg                                                                                                                                                  >  1:444237/1‑231 (MQ=255)
                                                                                   aTCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGGGGGT‑TGTT‑AAAAAGCATTTTT‑GACCTTCCGATGAAAA‑TGGGGGGGGATAAACCAGGCgggccggggg                                                                                                                                                  >  1:189801/1‑236 (MQ=255)
                                                                                                   gCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCATTGTT‑GACCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGc                                                                                                                                 <  1:270864/239‑1 (MQ=255)
                                                                                                    ccTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACg                                                                                                                                                                                                         <  1:1220860/169‑1 (MQ=255)
                                                                                                     cTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACg                                                                                                                                                                                                         <  1:1984062/168‑1 (MQ=255)
                                                                                                       gggTACTGTGGTGGTGGGGGGCGGGGTTAATGTTCGGGGTGGACAGGTTTGATGTCAGG‑ATCGGGGCACAAAGGCGGGTGAGGGGAGCAAATAGCTTCTCCCTGCTGGCACTTCCT‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGGGGTT‑TGAC‑AAAACGCATTGTT‑GACCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCt                                                                                                                             <  2:247038/239‑1 (MQ=255)
                                                                                                              gttgttgtGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑TTATGAAT‑GCGGGCGGCC‑TGTC‑AAAACGCAttgtt‑ggc                                                                                                                                                                                              >  2:1220860/1‑167 (MQ=255)
                                                                                                              gttgttgtGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑TCATGAAT‑GCGGGCGGCC‑TGTT‑AAAACGCCTTGTT‑TACCTGCCGATGAAAC‑TGGGGGGGCCTAAACCAGGCGGGCCGGGGTGACG‑CCGCGTGCTGGCAGcctaggata                                                                                                                      >  2:699374/1‑231 (MQ=255)
                                                                                                               ttgttgtGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGGGAAA‑TCATGAAT‑GCGGGGGGCT‑TGTT‑ATAAACCCTTGTT‑Gaa                                                                                                                                                                                              >  2:1984062/1‑167 (MQ=255)
                                                                                                                     tgCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTTTTTGTGCTGGGGGGTGAAA‑TCATGAAG‑GCGGGCGGCT‑TGTC‑AAAACGCATTGTT‑GCCCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCggg                                                                                                                                                         <  1:585637/198‑1 (MQ=255)
                                                                                                                       cGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCAt                                                                                                                                                                                                      >  1:2191071/1‑153 (MQ=255)
                                                                                                                       cGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCAt                                                                                                                                                                                                      <  2:2191071/153‑1 (MQ=255)
                                                                                                                                     gtttCTGGCTGGTCATGTTTG‑TGTTCAGTCTCTGGCCACAACCGCTGGTAACCGTGGCCGATAGCTTCTCCCTGCTGGCTATTCCG‑TTTTTTGTGCTGGGGGGGGAAA‑CCATAAAT‑GCGGGCGGTT‑TGTC‑AAAACGCATTGTG‑GCCCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑TCGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGcc                                                                                               <  2:485629/237‑1 (MQ=255)
                                                                                                                                            gctggACATGTTTGATGTCCAG‑ATCATGGCACAAACGCGGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCATTGTT‑GACCTGCCGATGa                                                                                                                                                                                    <  1:187489/149‑1 (MQ=255)
                                                                                                                                                             tCCAA‑ATCATGGCACAAACGCTGTGGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAACCGCATTGTT‑GCCCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTc                                                                                                         <  1:576918/205‑1 (MQ=255)
                                                                                                                                                                        ggCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGTTTCCG‑TTTTTTGTGCTGGCGGGTAAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCATTGTT‑GCCCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccg                                                                               <  1:1497330/221‑1 (MQ=255)
                                                                                                                                                                           ccaagctggtgTGACCGGCCCCGATCGTTTCTCCCTGGTCGCACTTCTT‑TTTTTTGTG‑TGGGGGGTTAAACTCATGAAG‑GCGGACGCTT‑TTTA‑AACACGCATTGTT‑GCCCTGCCGATAAAAC‑TGGTGGGGAATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGGGCCTTTCTGGTTCTGCCGTTGCGGATGCCGCCGCCGTTGCCGCTCTACTGGTGc                                                          <  2:1441573/229‑1 (MQ=255)
                                                                                                                                                                                          ggggcacGATAGTTCCCCCCGGGTGGC‑ATTCCGTTTTTTTGTGGTGGGGGATGAAG‑TAAGGAAG‑GGGGGGGTTT‑TGTA‑AAAACGCTTTGTT‑GCCCTGCCGATGAACC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAg                                             <  2:1442205/231‑1 (MQ=255)
                                                                                                                                                                                                      cTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑aaa                                                                                                                                                                                                            <  1:314071/69‑1 (MQ=255)
                                                                                                                                                                                                       ttCCCCGTGGTGGCTATCCCG‑TTTTTTGGGTGGGGGGTTAAAA‑TCAGGATG‑GCGGGGGGTT‑TGTA‑AAAACGCATTGTT‑GCCCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGt                              <  1:1159249/239‑1 (MQ=255)
                                                                                                                                                                                                              tgctgGCGATTCCG‑TTCTTTGTGCTGGGGGGTAAAA‑TCATGAAG‑GCGGGGGGTT‑TGTA‑AAAACGCATTTTT‑GACCTGCCGATGAAAC‑tggt                                                                                                                                                                            <  2:2105686/91‑1 (MQ=255)
                                                                                                                                                                                                                ctgGCGATTCCG‑TTCTTTGTGCTGGCGGGGGGAA‑ACATGAAT‑GGCGGCGGCT‑TGTT‑AAAAACCAttgtt                                                                                                                                                                                                  >  2:314071/1‑69 (MQ=255)
                                                                                                                                                                                                                    cGATTCCG‑TTCTTTGTGCTGGCGGGGGAAA‑TCATGa                                                                                                                                                                                                                                  >  1:1595451/1‑36 (MQ=255)
                                                                                                                                                                                                                       ttCCG‑TTTTTGGTGCGGGGGGGTAAAA‑TCATGAAT‑GCGGGGGGCT‑TGTC‑AAACCGCTTTGTT‑GACCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGt                                                                           <  1:609937/178‑1 (MQ=255)
                                                                                                                                                                                                                        tCCG‑TTCTTTGTGCTGGCGGGGGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCATTGTT‑GACCTGCCGATGAAAC‑TGGGGGGGCATaaa                                                                                                                                                                  >  1:2105686/1‑91 (MQ=255)
                                                                                                                                                                                                                           g‑TTTTTTGTGCGGGGGGGTAAAA‑TCATGAGT‑GCGGGGGGCT‑TGTC‑AACACGCATTGTG‑GACCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGa          <  1:1358660/239‑1 (MQ=255)
                                                                                                                                                                                                                                     gCTGGGGGGAAAAA‑TCATGAAG‑GGGGGGGGTT‑TGTC‑AAAACGCTTTGTT‑GCCCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATCCCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCctct  <  1:1705772/238‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         
AGCAATTCTCTAACGTCGCCACCGCAAGGAGCTTAATCATGGCTGTGCTGATTTTTCTGGGCTGTCTGTTGGGTGGTATTGCTATCGGTTTG‑CCTATTGCCTGGGCACTGTTGTTGTGCGGGGCGGCGTTAATGTTCTGGCTGGACATGTTTGATGTCCAG‑ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCG‑TTCTTTGTGCTGGCGGGTGAAA‑TCATGAAT‑GCGGGCGGCT‑TGTC‑AAAACGCATTGTT‑GACCTGCCGATGAAAC‑TGGTGGGGCATAAACCAGGCGGGCTGGGCT‑ACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCT  >  NC_000913/3744766‑3745229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑