Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,760,384 | 0 | A | C | 32.8% | 110.8 / 10.1 | 65 | V289G (GTG→GGG) | selA | selenocysteine synthase |
Reads supporting (aligned to +/- strand): ref base A (40/3); new base C (3/18); total (44/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.04e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
GCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCG > NC_000913/3760147‑3760609 | gCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTTCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCa > 1:2144923/1‑238 (MQ=255) tttGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AAAAATAATTCCTGCCTGCGGCCCGCCT‑AACAAATTTTCGCC‑GGGGAAACTCACCAg > 1:1581169/1‑235 (MQ=255) ctgcgctgcgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGTCCGCCT‑AACAACATTTCGCC‑CGGGTAAATCACCCGCATTAcgccccgccc > 2:923236/1‑233 (MQ=255) gcgctgcgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTACC‑‑AACAAAAATTCCTGCCCGCGGCCCGCCC‑CAAAACTTTTCGCC‑GGAGAAACACACCAGACCGAcgcccgcccccat > 2:2239666/1‑234 (MQ=255) cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTTCC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCCGACTCACGCCCGCCGCAATCCACt > 2:97170/1‑238 (MQ=255) cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTATC‑‑CACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTTTTGCC‑GGAGAAAATCACCAGACTGACGCCCGCCGCAATCAACt > 1:2235754/1‑238 (MQ=255) cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTTTCGCC‑GGAGAAAATCACCAGACTGACGCCCGCCGCAATCAACt > 1:1656371/1‑238 (MQ=255) cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAAATTTTTCCC‑GGAGAAACTCACCAGACTGACCCCCGCCGCAATCAACt > 1:93133/1‑238 (MQ=255) gcAGGGGCGGTAATTTTTCACTCAGAGCTTCAGGGAGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGTGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGAGGATTTGCAGGCGGGCGATCCTCCCTTTTTTTCC‑‑AAGCACAATTCCTCCCTGGGGCCCACCT‑AACAACTTTCCCCC‑GTCCGAACTCTACAGAACACCACCCGCCGCAAACAACTCCTGCGGGCTTgg > 2:227082/1‑238 (MQ=255) tgTATTTTTTCACTAAGAGCTCCAGGGTGAAAATAAAGCCGCAAGGTGGCTCCCAGCGCGGGGAGGTTCTTTTTACCCGCTCTTAAGCCACGCTTCGGGGGGTGGTTTTGCGGGCGGGCGACCATCTTTTTTTTACA‑‑AAAAATAATCCCTGCCTGGGCCCCGCCA‑AACACCTTGCCGCG‑GGAAAACCCCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCg < 2:614654/237‑1 (MQ=255) ttttCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTTCC‑‑AACAATAATTCCTGCCTGCGGCCCGCCC‑AACAACCTTTTGCC‑GGAGCAACTCACCAGACTGACGCCCGCCCCCATCAACTCCTGCGGCATCGGCCCTTTCGGCcaaac > 2:1858669/1‑237 (MQ=255) ttttCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTTCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCCACTCCTGCGGCATTGGCTCTTTCGGCCAAcc > 1:1831394/1‑239 (MQ=255) ttCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCCCTTTCGGCAAAcccg > 1:614654/1‑237 (MQ=255) tcccTCAGAGCTTCAGGGTTAAAAAAAAGCCGCAAGGTGGCTCCCGGCGCCGGGGGGGTCTTTTTATCCGCGGTTAATGCCCGCTTCAGCGGGTGGTTTTGCGGGCGGGCGTTCATCTTTTTTTTCCC‑‑AAAAAAAATCCCTGCCGGCGCCCCCCCT‑AACAACTTGTCGCC‑GAAAAAACCCCCCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑a < 2:1041159/236‑1 (MQ=255) cTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACCGACGCCCGCCCCAATCAACTCCTCCGGCCTTGGCTCTTTCGGCCAAcccg > 1:1986586/1‑233 (MQ=255) tgtAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGTTCACCTCTTTTTTACC‑‑AACAAAAATTTCTTCCTGCGGCCCGCCT‑AACAACTTTTCGCC‑GGAGAAACTCAACACACTTACGCCCGCCGCCATCAACTCCTGCCGGATTGGCTCTTTCTGCCAAACGT‑ACTCGCCAAGATCGACCa > 2:552925/1‑238 (MQ=255) aaataaaGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCg > 1:1428176/1‑238 (MQ=255) cGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGGGGCTTTGCAGGCGGGCGCTCATCTCTTTTTTACC‑‑AACCAAAATTCCTGCCCGGGGGCCGCCG‑AACAACTTTGCCCC‑GGAGAAAATCACCACAACGACTCCCGCCGCAAACACATCCCCCGGCAATGGCTCTTTTGGCAAAACGTAACTGCCCAA‑AACCACCACCGAGCCAcaccc > 2:1328576/1‑235 (MQ=255) aaCGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCCTCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCCTTGGCCCTTTCGGCAAACCGT‑CCTGGCTAAGa > 1:1744624/1‑216 (MQ=255) aaCGTGGATTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGGGATCATCTCCTTTTTGCC‑‑AAAAATAATTCCCGCCTGCCGCCCGCCT‑CAAAACATGTCGCC‑GGAGAAACTCCCCAGACTGACGCCCCCCCCAATCCACTCCTCCGGCCGTCGCCCTTTCGGCAAAACGA‑ACAGGCCAAAACAGACCCCCCAGCCAcctcctta > 2:1569492/1‑232 (MQ=255) gggcgtttCCGGCCCCGCGGGGTTTTTTTTACCCGGCAGTAAGGCCCGTTTCGGGGGGGGGTTTGGCGGGGGGCCAATCTTCT‑TTTTTTACC‑‑AAAAAATATTCCCGGCCGGGGCCCCCCTAAACAACTTGCCGCC‑GGAAAACCCCCCCAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCa < 2:1889496/223‑1 (MQ=255) ttCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑CACAATAATTCCTTCCTTCGGCCCGCCT‑AACAACTTGTCGCC‑GGGGAAACTCCCCAGACCGGCGCCCGCCGCAATCAACCCCTGCGGCATTGGCCCTTTTCGCTAACCGG‑ACTGGCTAAAAACGGCCAGCGACCCACCGCCTCaatcaatc > 2:1220990/1‑238 (MQ=255) cgccgcGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTTTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCCAACCGT‑ACTGGCTAAGATCGACCCGCGAGCCACTGCCTAAAt > 1:1889496/1‑227 (MQ=255) cgcgAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCCGTCATCTCTTTTTTTCC‑‑AACAATAATTCCTGCCTGCGGCCCGCCC‑AACAACTTGTCGCC‑GGAGAAACTCACCCGACTGACCCCCGCCCCAATCCACCCCTGCCGCCTTTGCTCTTTCGGCCAACCGC‑ACTGGCTAAGAACGAACACCGAGCCACCGCCTCAATCAGTCACCACgggga > 2:2127908/1‑237 (MQ=255) cATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTTCC‑‑AACAATAATTTCCGCCCTGGGCCCGCCC‑CACAACTTGGTCCC‑GGAGAAAATCCACCGACCGACGCCCGCCGCAATCAAATCCTGCGGGGTTGGCTCCTTCGGCAAAACGT‑AATGGCTAAAATCGACCCACGAGCCCCAGCCCATAACAGTCACTACAGGGAAATCCCGc > 2:205839/1‑238 (MQ=255) tCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCACCTCTTTTTTTAC‑‑CACAAAAATTTCTGCCTGCGGCCCGCCT‑AACAAACTGTCGCC‑TGAGAAAATCACCAGACTGACGCCCCCCGCAATCAAACCCTGCGGCATTGGCTCTTTTGGCAAACCGT‑ACTGGCTTACATAGACAAACGAGACCCCGCCGAAATAAATCACCAATGGGACATACATATCTTTTcc > 2:1183347/1‑239 (MQ=255) cgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTTTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAgcg > 1:1819086/1‑238 (MQ=255) cccGCTCCCGCGGGTGCTTTTGCGGGGGGCCATTCCTTTTTTTTTCACC‑‑AAAAAAATTCCCTGCCGCCGCCCCCCAT‑AACCACTTGCCGCC‑GAAAAACCCCCCCAAACGACCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAgc < 2:1693542/228‑1 (MQ=255) tCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGAc > 1:135892/1‑169 (MQ=255) aGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTTCC‑‑CACAATAATTCCTGCCTTCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACCGACGCCCGCCGCAATCAACTCCTCCGGCATTGGCTCTTTCGGCCAAACCT‑ACTGGCTAAGATCGGCCAGCCAGCCCCCGCCCAAATCAGCTACCAAGGGAACCACCAGCCCTTTTCCCAGCGCCCCCACTTTCGcct > 2:1689931/1‑236 (MQ=255) cGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTTCC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTTTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCCGCAAACCGT‑ACTGGCTAAGAACGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACAACCAGACCTTTGTCCAGCGCCCCCAGATcc > 2:1802090/1‑232 (MQ=255) cGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTTCCTGCGGCCCGCCT‑AACAACTTTTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCCGtt > 1:1693542/1‑230 (MQ=255) ggCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATc > 1:1816410/1‑239 (MQ=255) tGCCGGGGGGCCATCATCTTTTTTTTACC‑‑AAAAAACATCCCCCCCCGCCGCCCCCCT‑AAACACTGTGCGCCGGGAAAACCCCACCAGCCGACCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCtt < 2:505964/239‑1 (MQ=255) gggggcgcttctcttTTTTTTCCA‑‑AAAAATAACCCCCCCCCGCGGCCCCCAA‑AACATTTTGCCGCG‑GGAAAACCCCCCCACACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTg < 2:2159718/227‑1 (MQ=255) gcgggcgACCTTCTCTTTTTACCC‑‑AAAAATAATTCCTCCCGGCGGCCCCCCT‑AACAACTTGCCGCG‑GGAAAAACTCCCCAGACTGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTg < 1:2239666/238‑1 (MQ=255) gcgggcgAACTTTTTTTTTAAACA‑‑AACAATAATCCCGGCCTGCGCCCCGCAA‑ACCAACTTGCCGCG‑GAAAAACCCCCCCAGCCGGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTg < 2:753329/238‑1 (MQ=255) cgATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCCATCAACCCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCTACCAGCGAGCCACCGCCTAAATCAGTCACTACCGGAACATCCAGCCCTTTGCCGACCGCCCCCAGTTCCGCTTCATCTATCGCCTTGGTGAAcc > 2:1081619/1‑237 (MQ=255) caatcTTTTTTTCACC‑‑AAAAAAATTCCCTGCCGGCGGCCCCCCT‑AACAACTAGCCGCC‑GGAAAACCCCACCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGa < 1:2100868/237‑1 (MQ=255) tcctctTTTTTACCCAAACAATATTCCCGCCCGGCGCCCCGCCA‑AAAACCTGGTCCCG‑GGAAAAACTCCCCAGCCGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGa < 2:1986586/237‑1 (MQ=255) ttttttttCCA‑‑AAAAATAATCCCTGCCTGCGGCCCCCCT‑AACAACTTGGCGCG‑GGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCt < 1:944979/237‑1 (MQ=255) ttttttACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAAt > 1:1547747/1‑155 (MQ=255) tttACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGt > 1:1766583/1‑239 (MQ=255) tccaaaaaattatCCCTCCCGGCGCCCCCCCT‑AACATCTTGTCGCG‑GGAGAACCCCCCCAACCTCCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa < 1:205839/232‑1 (MQ=255) acaaTATTCCCTGCGTGCGGCCCGCCA‑AACATCTTGGCCCC‑GGAAAACCCCCCCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt < 1:97170/239‑1 (MQ=255) acaaTAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt > 1:1808670/1‑239 (MQ=255) acaaTAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGCTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt > 1:424369/1‑239 (MQ=255) aaaataatCCCTCCCGGCGGCCCGCCT‑AACACCTTGTCGCG‑GGAGAAACCCCCCAGGCGGGCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt < 1:1569492/237‑1 (MQ=255) aataatTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTAt > 1:1817395/1‑239 (MQ=255) ataatCCCTGCCGGCCCCCCGCAT‑AACAATTGGGCGCC‑GGAAAACCGCACAATGCGGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATg < 2:1674832/239‑1 (MQ=255) attcccggccggcGGCCCCCCA‑AACAACTTGCCGCG‑GGAAAACCCCCCAAGGCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa < 1:923236/232‑1 (MQ=255) tccctgcctgCGCCCCCCCA‑AACACCTTGCCCCC‑GGAGAACCCCCCCACCCGAACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑a < 2:787776/101‑1 (MQ=255) tCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCa > 2:101784/1‑44 (MQ=255) tCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCa < 1:101784/44‑1 (MQ=255) ccctgcctgCGCCCCCCCA‑AACAACTTGTCGCC‑GGAAAACCCCCCCAGCCGGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑a < 2:1420018/101‑1 (MQ=255) cctgcctgCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGt > 1:1240894/1‑234 (MQ=255) gcctgcGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca > 2:1647666/1‑238 (MQ=255) gcctgcGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca > 2:1495516/1‑238 (MQ=255) cGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGa > 1:787776/1‑103 (MQ=255) ggCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGa > 1:1420018/1‑102 (MQ=255) cT‑AACAACTTGCCGCC‑GGAAAACCCCCCCAGACGGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑a < 2:627024/85‑1 (MQ=255) ccactttgcGCC‑GGAAAAACTCCCCAGCCTGGCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTAtttt < 1:1245359/231‑1 (MQ=255) tcctggTCGCC‑GAAAAACCCCCCAAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa < 2:1897480/209‑1 (MQ=255) aacTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGAATTttc > 1:1032628/1‑239 (MQ=255) gTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGa > 1:627024/1‑85 (MQ=255) tCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGAGGTATTttcattca > 1:596656/1‑238 (MQ=255) c‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCaa > 1:1897480/1‑214 (MQ=255) c‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCg > 1:1276281/1‑237 (MQ=255) | GCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACC‑‑AACAATAATTCCTGCCTGCGGCCCGCCT‑AACAACTTGTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGT‑ACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCG > NC_000913/3760147‑3760609 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |