Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913417,5330TG66.7% 3.7 / 28.5 51V131G (GTG→GGG) phoBresponse regulator in two‑component regulatory system with PhoR
Reads supporting (aligned to +/- strand):  ref base T (10/7);  new base G (34/0);  total (44/7)
Fisher's exact test for biased strand distribution p-value = 1.68e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.24e-05
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGAGATGGGGCCGA‑CAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGC  >  NC_000913/417303‑417768
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
gATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGCGGGGATTgaga                                                                                                                                                                                                                                          >  1:831749/1‑239 (MQ=255)
      aCCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAgg                                                                                                                                                                                                                                    >  1:1718415/1‑239 (MQ=255)
       ccTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAggg                                                                                                                                                                                                                                   >  1:2153737/1‑239 (MQ=255)
       ccTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAGGAGGGGGTTGAGAGGCgggg                                                                                                                                                                                                                                   >  2:420024/1‑239 (MQ=255)
       ccTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAggg                                                                                                                                                                                                                                   >  1:1571030/1‑239 (MQ=255)
          ggcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGGTTGAGATGCAGGGAtt                                                                                                                                                                                                                                >  1:1166727/1‑239 (MQ=255)
               cTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGCGGGGGGATTGGGGTGCAgggggttag                                                                                                                                                                                                                             >  2:917115/1‑231 (MQ=255)
                tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑Gtc                                                                                                                                                                                                                          >  1:1782730/1‑238 (MQ=255)
                 ccGTTATCCATTTACACAAACACCCCAAGCGCGAGTCGATGACCCGGGATAGTCCGGTGGTTGTGTGGGCCGCCTGCGGGGAAGAAGAGGAGCGCGTGCGCGCCCTTGAAATCGGCGCGGATCGCTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGTGGTGATTGAGTTGCAGGGATTAA‑Gtctc                                                                                                                                                                                                                        <  2:2017659/239‑1 (MQ=255)
                            ttCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGGTTAA‑GGCTCGACCCGACa                                                                                                                                                                                                               >  1:1358767/1‑237 (MQ=255)
                            ttCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGGATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAA‑GCCTCGACCCGACAt                                                                                                                                                                                                              >  1:485709/1‑238 (MQ=255)
                            ttCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGCGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCG‑TATCGCCGCAGGGGCTGGTGGCGCGAATCAAAGCGGTAATGGGCCGTATTTCGCCAATGGCGGGGGGTGGGGGGGTTGAGATGCAGGGGTAAA‑GATCCGACCCGACAtc                                                                                                                                                                                                             >  2:1529575/1‑238 (MQ=255)
                              catcaAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGGGATTGAGATGCAGGGATTAA‑GTCTTGAACCGACAtctc                                                                                                                                                                                                           >  1:2076562/1‑239 (MQ=255)
                              catcaAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGGTTAA‑GTCTCGAACCGACAtctc                                                                                                                                                                                                           >  1:841663/1‑239 (MQ=255)
                               atcaAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTCTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGGGGGGGTTGAGATGCAGGGATTAAGGTCT‑GACCCGACAtcca                                                                                                                                                                                                           >  2:1647708/1‑236 (MQ=255)
                                 caAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGCAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGCTGACTATATCACCAAGCCGCTTTCGCCGAAGGAGCTGTTGGCGCGAATCAAAGCGGCAATGCGCCGCATTTTGCCAATGGCGGTGGCAGAGTGGATTGAGGTGCAGGGAt                                                                                                                                                                                                                                 >  2:659174/1‑215 (MQ=255)
                                        cTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAAAGGGGATTGGGATGCAGGGATTAA‑GTCTCGACCCCGCATCTCCCCGGGTGAt                                                                                                                                                                                                 >  2:1641552/1‑239 (MQ=255)
                                          cAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATgg                                                                                                                                                                                               >  2:533229/1‑239 (MQ=255)
                                                cgAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGTGATGCAGGGATTAA‑GTCTCGACCCGCCCTCCCACCGCGTGATggg‑‑ggggc                                                                                                                                                                                       >  2:1666192/1‑237 (MQ=255)
                                                  aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGCGGGGAAGAAGAAGATCCCGTGCGCGGCCTTGGAACCGGCGCGGATGACTATATCACCCAGCCGCTTTCGCCGGAGGAGCTGGGGGCGCGAATCAATGCCGTAATTCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGGATTAGATGCACGGGGTAA‑GTCTCTACCCCGGACCTCACCGAGGGATgggaggggag                                                                                                                                                                                       >  2:1730722/1‑231 (MQ=255)
                                                  aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCATCGCGGTGTggc‑‑ggggcgc                                                                                                                                                                                     >  2:37759/1‑235 (MQ=255)
                                                  aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGGGATggc‑‑gggcgga                                                                                                                                                                                     >  1:726801/1‑237 (MQ=255)
                                                  aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCAGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGGGATGGC‑‑GGGCGaa                                                                                                                                                                                     >  1:1974128/1‑239 (MQ=255)
                                                        tGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAA‑GGCTCGACCCGACATCTCACCGAGTGATggg‑‑gggcggggaggc                                                                                                                                                                                >  2:2134793/1‑231 (MQ=255)
                                                                 atatTCCAGTGGTGATGTTGACCGCCGGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGGGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGAGAt                                                                                                                                                                      <  2:500195/238‑1 (MQ=255)
                                                                     tCCAGTGGTGATGTTGACCGCCAGTGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGGGGGGATTGCGAATTAGGGGCTCA‑GGCTCTACCCGCCACTTCACCCCGGTGTGGC‑‑GGCCGTAGAGACG‑CCGCAGATaggg                                                                                                                                                                  >  2:1568675/1‑238 (MQ=255)
                                                                     tCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGGGATGGC‑‑GGGGGGAAAGCCG‑CTGGGGATgggg                                                                                                                                                                  >  1:567386/1‑238 (MQ=255)
                                                                         gtggtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGGGGGATGGC‑‑GGGCGGAAAGCCG‑CCGGGGATTGGGCCgcc                                                                                                                                                             >  1:1242977/1‑237 (MQ=255)
                                                                            gtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGTGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGCGAGAGGCG‑CCGGGGGTGGGGCCgcgcga                                                                                                                                                          >  2:1344756/1‑234 (MQ=255)
                                                                               aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGGTTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGGGATGGG‑‑GGGGGGAGAGACG‑CGGGGGGAGGGGGCGA‑GAGaaat                                                                                                                                                      >  2:1595691/1‑237 (MQ=255)
                                                                               aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGGGTGATGGC‑‑GGGGGGAGAACCG‑CCGGGGGAGGGGCCCA‑CCGaaat                                                                                                                                                      >  1:1870220/1‑237 (MQ=255)
                                                                               aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGGGGTTGGGATGCAGGGGTTAA‑GTCCCGAACCGACATCTCACCGAGGGGAGGCG‑GGGCGAGGAGCCG‑CTGGGGGGGGGGcggcagacat                                                                                                                                                        >  2:879628/1‑230 (MQ=255)
                                                                                 gTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATGGCG‑GGGGGAAGGGCC‑‑CCGGGGAGGGGGCCGG‑CAGAAtttt                                                                                                                                                    >  1:1745334/1‑238 (MQ=255)
                                                                                      ccGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGGGGGGGTTGGGATGGAGGGGTTAA‑GACTCGACCCGGCATTCCACCGGGGGGTGGG‑‑GGGCGGAGAGACG‑cgcggggggg                                                                                                                                                                    >  2:632155/1‑210 (MQ=255)
                                                                                      ccGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGGGATGGC‑‑GGGGGGAGAGGCG‑CCGGGGATGGGGCCGA‑CAGGAtttta                                                                                                                                                   >  1:622106/1‑233 (MQ=255)
                                                                                      ccGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCCGGGATTAA‑GTCTCGACCCGACATCTTACCGAGTGATGGC‑‑GGGGGGAAGGCCC‑CGGGGGGTGGGGGCGG‑CAGAATTTTAAca                                                                                                                                                >  1:531996/1‑237 (MQ=255)
                                                                                         ccAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGGGGGGGTTGGGGTGCAGGGGTTAA‑GGCTCGACCCGACCTCCCACCCAGGGATGGCG‑GGGGGAAGGACCG‑CGGGGGGTGGGGCCTG‑CAAAATTAAAActggt                                                                                                                                             >  2:826321/1‑237 (MQ=255)
                                                                                            gagGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGGATGAGATGCAGGGGTTTA‑GGTCCGACCCGACATCTCACCGAGGGGTGGCGGGGGCGGAGAGCCG‑CGGGAG‑GGGGGCCGC‑CCGAATTTacaagagtt                                                                                                                                            >  2:1948995/1‑229 (MQ=255)
                                                                                                  aagaagaagATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGTGAATGAGATGCAGGGGTTTA‑GTCTTGACCCGACCTCCCACCGAGTGATGGCG‑GGGGGAACAGCGC‑GTGGGGAGGGGGGCGA‑AGAAATTTAAACTCATACACttttt                                                                                                                                    >  2:1410155/1‑239 (MQ=255)
                                                                                                    gaagaagaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGGGATGGC‑‑GGGGGAAGAGCCG‑CCGGGGATGGGGCCGACCAGAATTTAAATTG‑TGCACcttttttt                                                                                                                                 >  1:1544135/1‑237 (MQ=255)
                                                                                                                                                        aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGGGAGAGCCG‑CCGga                                                                                                                                                                         >  1:2189317/1‑148 (MQ=255)
                                                                                                                                                        aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGGAAAGCCG‑CCGGAGATGGGGCCGA‑CAGAAATTTAAATGCTGCACTTTTTTTTTGCGCCACCTGGGGGCGTGTATCGCCCCGGGGCGCCGTTTAACCAAGTCTgg                                                                             >  2:1153593/1‑239 (MQ=255)
                                                                                                                                                        aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑GTCTCGACCCCACATCTCACCGAGTGATGGC‑‑GGGGGGAGAGGCGCCGGGGGGTGGGGCGGC‑CAGATTTTAAA‑TGCTGCCCCTTTTTTTTACGCCACCTCTGCGCGGGTTTCACCCCGCGCAGCTGTTAAAacacaccttg                                                                             >  2:838923/1‑233 (MQ=255)
                                                                                                                                                                    cGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑Gt                                                                                                                                                                                                                           >  1:89206/1‑89 (MQ=255)
                                                                                                                                                                                                                    cAATGGCGGTGGAAGAGGTGAtt                                                                                                                                                                                                                                              <  2:221408/23‑1 (MQ=255)
                                                                                                                                                                                                                      aTGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGGGAGAGGCG‑CCGGGGGTGGGGGCGG‑CAGAATTTaaaat                                                                                                                                                >  1:728139/1‑108 (MQ=255)
                                                                                                                                                                                                                      aTGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCCCCGAGGGATGGC‑‑GGGGGGAGAGCCG‑CCGGGGAAgggg                                                                                                                                                                  >  2:2133539/1‑93 (MQ=255)
                                                                                                                                                                                                                            gTGGAAGAGGTGATTGAGATGCa                                                                                                                                                                                                                                      <  2:1825492/23‑1 (MQ=255)
                                                                                                                                                                                                                            gTGGAAGAGGTGATTGAGATGCa                                                                                                                                                                                                                                      <  2:89206/23‑1 (MQ=255)
                                                                                                                                                                                                                             tGGAAGAGGTGATTGAGATGCAg                                                                                                                                                                                                                                     <  2:66840/23‑1 (MQ=255)
                                                                                                                                                                                                                                    ggTGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGAGATGGGGCCGA‑CAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCcggc  <  2:1365508/238‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
GATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAA‑GTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGAGATGGGGCCGA‑CAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGC  >  NC_000913/417303‑417768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑