Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,595,496 G→A A119V (GCG→GTG)  lgoR ← putative transcriptional activator for L‑galactonate catabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,595,4960GA100.0% 294.9 / NA 75A119V (GCG→GTG) lgoRputative transcriptional activator for L‑galactonate catabolism
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/37);  total (38/37)

GCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGC  >  NC_000913/4595263‑4595727
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       
gCCGATGACGTTCGAGCGTGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGcc                                                                                                                                                                                                                                    <  1:549658/239‑1 (MQ=255)
   gATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGa                                                                                                                                                                                                                                 <  1:470657/239‑1 (MQ=255)
       aCGTTCGCGCATGGTTTTCGCCTGCAGCCAGCGGGTATCAAGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGcc                                                                                                                                                                                                                              <  2:559792/238‑1 (MQ=255)
        cGTTCGAGCATGGTTTTCGCCTGCAGCCCGCGGGGATCCTGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCTCGCTTTTCGCTGTGAATAACATTATAACGGCCGAATTTTAAACGACATTCTGTTCATACAACCGGGCTTAGACCCACGACCCGAGcc                                                                                                                                                                                                                              >  2:491035/1‑237 (MQ=255)
                         cGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCTCGAACCAGTTGCAGTTCAGcaaaag                                                                                                                                                                                                           >  2:289822/1‑237 (MQ=255)
                         cGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACg                                                                                                                                                                                                           >  2:123158/1‑239 (MQ=255)
                                   cagcGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTccc                                                                                                                                                                                                     <  2:1014260/235‑1 (MQ=255)
                                   cagcGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTccc                                                                                                                                                                                                     <  1:254550/235‑1 (MQ=255)
                                                gATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcgc                                                                                                                                                                                    <  1:854979/239‑1 (MQ=255)
                                                      gcaggTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTACCCTGGGCGTAACTGGGCCTGGttt                                                                                                                                                                              >  2:854979/1‑239 (MQ=255)
                                                                    tGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGaaaaa                                                                                                                                                                >  1:728069/1‑239 (MQ=255)
                                                                     gCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGaaaaaa                                                                                                                                                               >  1:617503/1‑239 (MQ=255)
                                                                        gAAGTTAATTTGTTTCGAGCATTTCGCGTAGCTCAAGCAGCTGCTCGGCGTAGGATTGATCGAATTGATGCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                                                                                            <  2:617503/239‑1 (MQ=255)
                                                                                               tCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTc                                                                                                                                     <  2:728069/239‑1 (MQ=255)
                                                                                                     aGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcgc                                                                                                                                                                                    >  2:273835/1‑186 (MQ=255)
                                                                                                     aGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcgc                                                                                                                                                                                    <  1:273835/186‑1 (MQ=255)
                                                                                                        tCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGa                                                                                                                            <  1:123158/239‑1 (MQ=255)
                                                                                                                 tgctCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCAc                                                                                                                                   <  2:700597/223‑1 (MQ=255)
                                                                                                                 tgctCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCAc                                                                                                                                   >  1:700597/1‑223 (MQ=255)
                                                                                                                          tAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcg                                                                                                                                                                                     >  2:1016105/1‑164 (MQ=255)
                                                                                                                          tAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcg                                                                                                                                                                                     <  1:1016105/164‑1 (MQ=255)
                                                                                                                                  aTCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTg                                                                                                                                                                                                                         <  2:20332/120‑1 (MQ=255)
                                                                                                                                  aTCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTg                                                                                                                                                                                                                         >  1:20332/1‑120 (MQ=255)
                                                                                                                                  aTCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                                                                                     >  1:561037/1‑188 (MQ=255)
                                                                                                                                  aTCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                                                                                     <  2:561037/188‑1 (MQ=255)
                                                                                                                                    cGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatca                                                                                                >  1:209004/1‑239 (MQ=255)
                                                                                                                                       aTTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcgc                                                                                                                                                                                    >  1:953714/1‑152 (MQ=255)
                                                                                                                                       aTTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcgc                                                                                                                                                                                    <  2:953714/152‑1 (MQ=255)
                                                                                                                                                tGCTCCACTGGCCGGGCTTTGCGGTGTGAATAAGGTTGTAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCg                                                                                                                                                                       <  1:897277/156‑1 (MQ=255)
                                                                                                                                                tGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatc                                                                                                 >  2:550632/1‑226 (MQ=255)
                                                                                                                                                tGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatc                                                                                                 <  1:550632/226‑1 (MQ=255)
                                                                                                                                                     cACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTcccc                                                                                                                                                                                                    >  2:271997/1‑122 (MQ=255)
                                                                                                                                                     cACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTcccc                                                                                                                                                                                                    <  1:271997/122‑1 (MQ=255)
                                                                                                                                                     cACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACgcgc                                                                               >  2:232305/1‑239 (MQ=255)
                                                                                                                                                        tGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGt                                                                                                                                                                                                                     >  1:371581/1‑102 (MQ=255)
                                                                                                                                                        tGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGt                                                                                                                                                                                                                     <  2:371581/102‑1 (MQ=255)
                                                                                                                                                           ccGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAAc                                                                         >  1:11285/1‑239 (MQ=255)
                                                                                                                                                               gcTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgc                                                                                                                                                                                      <  2:587061/126‑1 (MQ=255)
                                                                                                                                                               gcTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgc                                                                                                                                                                                      >  1:587061/1‑126 (MQ=255)
                                                                                                                                                                                      taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcg                                                                                                                                                                                     >  1:360132/1‑104 (MQ=255)
                                                                                                                                                                                      taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcg                                                                                                                                                                                     <  2:360132/104‑1 (MQ=255)
                                                                                                                                                                                      taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                                                                                            >  1:883567/1‑129 (MQ=255)
                                                                                                                                                                                      taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                                                                                            <  2:883567/129‑1 (MQ=255)
                                                                                                                                                                                           gCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTg                                                                                                                                                                                                                         >  1:774340/1‑63 (MQ=255)
                                                                                                                                                                                           gCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTg                                                                                                                                                                                                                         <  2:774340/63‑1 (MQ=255)
                                                                                                                                                                                            ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                                                                                            >  1:827896/1‑123 (MQ=255)
                                                                                                                                                                                            ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                                                                                            <  2:827896/123‑1 (MQ=255)
                                                                                                                                                                                            ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtt                                                                                                                                           >  2:256052/1‑140 (MQ=255)
                                                                                                                                                                                            ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtt                                                                                                                                           <  1:256052/140‑1 (MQ=255)
                                                                                                                                                                                            ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGt                                                                                            >  2:995989/1‑187 (MQ=255)
                                                                                                                                                                                            ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGt                                                                                            <  1:995989/187‑1 (MQ=255)
                                                                                                                                                                                                           tatTCTCTTACTACAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCt                         <  1:232305/239‑1 (MQ=255)
                                                                                                                                                                                                                   tactacAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAatat                 >  2:799693/1‑239 (MQ=255)
                                                                                                                                                                                                                    actacAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                                                                                            >  2:167766/1‑99 (MQ=255)
                                                                                                                                                                                                                    actacAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                                                                                            <  1:167766/99‑1 (MQ=255)
                                                                                                                                                                                                                    actacAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTc                                                                                                                                     >  2:389014/1‑122 (MQ=255)
                                                                                                                                                                                                                    actacAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTc                                                                                                                                     <  1:389014/122‑1 (MQ=255)
                                                                                                                                                                                                                    actacAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTc                                                                                                                                     <  1:265127/122‑1 (MQ=255)
                                                                                                                                                                                                                    actacAACCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTc                                                                                                                                     >  2:265127/1‑122 (MQ=255)
                                                                                                                                                                                                                         aaCCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                                                                                                >  2:147906/1‑106 (MQ=255)
                                                                                                                                                                                                                         aaCCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                                                                                                <  1:147906/106‑1 (MQ=255)
                                                                                                                                                                                                                          aCCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgc                                                                                                                                                                                      >  2:625184/1‑67 (MQ=255)
                                                                                                                                                                                                                          aCCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgc                                                                                                                                                                                      <  1:625184/67‑1 (MQ=255)
                                                                                                                                                                                                                          aCCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTttgttttgtt                                                                                                                                      <  1:494310/115‑1 (MQ=255)
                                                                                                                                                                                                                          aCCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTttgttttgtt                                                                                                                                      >  2:494310/1‑115 (MQ=255)
                                                                                                                                                                                                                          aCCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatc                                                                                                 >  2:490369/1‑152 (MQ=255)
                                                                                                                                                                                                                          aCCGGGCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatc                                                                                                 <  1:490369/152‑1 (MQ=255)
                                                                                                                                                                                                                              ggCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTaa                               >  1:529321/1‑214 (MQ=255)
                                                                                                                                                                                                                              ggCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTaa                               <  2:529321/214‑1 (MQ=255)
                                                                                                                                                                                                                              ggCTGACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgtg      >  2:846226/1‑239 (MQ=255)
                                                                                                                                                                                                                                  gACACCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGTGAATTATACGCACTGTGGTGc  >  2:676196/1‑239 (MQ=255)
                                                                                                                                                                                                                                   acacCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTccc                                                                                                                                                                                                     >  1:219861/1‑43 (MQ=255)
                                                                                                                                                                                                                                   acacCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTccc                                                                                                                                                                                                     <  2:219861/43‑1 (MQ=255)
                                                                                                                                                                                                                                   acacCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                                                                                  <  1:19265/158‑1 (MQ=255)
                                                                                                                                                                                                                                   acacCCACGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                                                                                  >  2:19265/1‑158 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       
GCCGATGACGTTCGAGCATGGTTTTCGCCTGCAGCCAGCGGGGATCATGATCAGGCAGGTTGAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGC  >  NC_000913/4595263‑4595727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: