Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,809,774 (T)7→6 coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7680T.97.8% 405.5 / 3.4 91coding (152/738 nt)ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base . (39/49);  total (41/50)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

TGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACC  >  NC_000913/2809671‑2809862
                                                                                                 |                                                                                              
tGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttt                                                                                              <  2:857343/100‑1 (MQ=255)
  cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttt                                                                                            >  1:1515133/1‑100 (MQ=255)
  cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttt                                                                                            >  1:261897/1‑100 (MQ=255)
   aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGtttttt                                                                                           >  2:2275439/1‑100 (MQ=255)
    aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttc                                                                                          <  1:1347518/100‑2 (MQ=255)
    aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttc                                                                                          <  1:2374724/100‑2 (MQ=255)
    aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttc                                                                                          >  1:2438720/1‑99 (MQ=255)
     aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttct                                                                                         <  2:1340007/100‑3 (MQ=255)
     aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttct                                                                                         <  1:2334272/100‑3 (MQ=255)
        cAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCt                                                                                     >  2:2005181/1‑100 (MQ=255)
         aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGTATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTg                                                                                    >  2:302018/1‑100 (MQ=255)
         aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTg                                                                                    <  2:993087/100‑1 (MQ=255)
         aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTg                                                                                    <  1:1891646/100‑1 (MQ=255)
          gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGc                                                                                   >  1:1224902/1‑100 (MQ=255)
          gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGc                                                                                   >  1:25814/1‑100 (MQ=255)
              aCCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGcatca                                                                               <  1:1344836/100‑1 (MQ=255)
                cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCAtt                                                                             >  1:1727430/1‑100 (MQ=255)
                 gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCAttt                                                                            >  2:234053/1‑100 (MQ=255)
                    tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATg                                                                         <  1:2168077/100‑1 (MQ=255)
                     gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGc                                                                        >  2:654371/1‑100 (MQ=255)
                     gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGc                                                                        <  2:1945807/100‑1 (MQ=255)
                     gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGc                                                                        >  2:1146485/1‑100 (MQ=255)
                     gTTATTAGTTATATTCCGGTGGCCTTTGCGTCCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGc                                                                        <  1:2483447/100‑1 (MQ=255)
                      ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCa                                                                       >  2:2072287/1‑100 (MQ=255)
                      ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCa                                                                       >  2:2560175/1‑100 (MQ=255)
                      ttataaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGTCCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATAAATGATGCa                                                                       <  2:2221922/100‑1 (MQ=255)
                        attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGATAGCGTTTTTTCTCCTGCATCATTTATGCAgg                                                                     >  2:2032472/1‑100 (MQ=255)
                        attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAgg                                                                     <  1:1051917/100‑1 (MQ=255)
                        attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAgg                                                                     <  2:810175/100‑1 (MQ=255)
                        attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAgg                                                                     >  2:2250433/1‑100 (MQ=255)
                          taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATAAATGATGCAggag                                                                   <  2:1877894/100‑3 (MQ=255)
                            gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGcgcg                                                                 >  1:686562/1‑100 (MQ=255)
                            gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGcgcg                                                                 >  1:1109017/1‑100 (MQ=255)
                              tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAg                                                               >  2:776936/1‑100 (MQ=255)
                                tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                             <  1:1965630/100‑1 (MQ=255)
                                tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCGCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                             <  2:776680/100‑1 (MQ=255)
                                 atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCCCGAGCCa                                                            >  2:1358288/1‑100 (MQ=255)
                                  ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                           <  1:1465706/100‑1 (MQ=255)
                                  ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                           <  2:2392952/100‑1 (MQ=255)
                                   tCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                          <  1:1198652/100‑1 (MQ=255)
                                    ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGtt                                                         <  1:806804/100‑1 (MQ=255)
                                     cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                        >  1:2586241/1‑100 (MQ=255)
                                     cGGTGGCCGTTGCGTTCGGTCAGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCACCATTTATGCAGGCGCGAGCCAGTTc                                                        >  2:1758293/1‑100 (MQ=255)
                                      ggTGGCCGTTGCGGTCGGTCTGATGGCTACCCGGCTGGGGTTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                       <  2:2108086/100‑1 (MQ=255)
                                      ggTCGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTTCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                       <  2:1064504/100‑1 (MQ=255)
                                       gTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGt                                                      <  1:1074712/100‑1 (MQ=255)
                                         ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTtcatca                                                    <  1:2032059/100‑1 (MQ=255)
                                         ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                                    >  2:1673587/1‑100 (MQ=255)
                                         ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                                    <  2:1872306/100‑1 (MQ=255)
                                         ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                                    <  2:825553/100‑1 (MQ=255)
                                         ggCCTTTGCGTTCGGTCTGAATGCGACCCGCCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                                    <  2:390348/100‑1 (MQ=255)
                                           ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                                  <  1:1502159/100‑1 (MQ=255)
                                           ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                                  >  1:338345/1‑100 (MQ=255)
                                            cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTa                                                 >  1:1536895/1‑100 (MQ=255)
                                               tGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                              >  2:155013/1‑100 (MQ=255)
                                                gCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                             >  1:2616416/1‑100 (MQ=255)
                                                  gatcagTCTGAATGCGCCGCGTCTGGGATTATCTCCTCTCGAAAGCCTTTTGTCTCCTTCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGa                                           <  2:2392731/95‑1 (MQ=255)
                                                  gTTCGGTCTGAATGTGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGa                                           <  1:1939447/100‑1 (MQ=255)
                                                  gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGa                                           >  1:816032/1‑100 (MQ=255)
                                                   ttCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGan                                          <  1:163123/100‑2 (MQ=255)
                                                      ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                       >  2:691998/1‑100 (MQ=255)
                                                      ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                       <  1:840168/100‑1 (MQ=255)
                                                      ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                       <  1:795150/100‑1 (MQ=255)
                                                       gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                      <  2:522436/100‑1 (MQ=255)
                                                       gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                      <  1:463555/100‑1 (MQ=255)
                                                        tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTTTTTTTTCATTTATGCAGGCGCGAGCCAGTTCGTCATTGCCGCGAGGCTgg                                     >  2:696353/1‑100 (MQ=255)
                                                        tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGCGCCAGTTCGTCATTACCGCGATGCTgg                                     <  2:767719/100‑1 (MQ=255)
                                                        tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                     <  1:493298/100‑1 (MQ=255)
                                                        tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                     <  1:1353984/100‑1 (MQ=255)
                                                        tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                     <  2:257199/100‑1 (MQ=255)
                                                           gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                                  >  2:1916744/1‑100 (MQ=255)
                                                           gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                                  >  2:2403628/1‑100 (MQ=255)
                                                                cGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                             <  2:41263/100‑1 (MQ=255)
                                                                cGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                             <  1:730598/100‑1 (MQ=255)
                                                                cGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                             <  1:1186909/100‑1 (MQ=255)
                                                                   cccGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagt                          >  2:967612/1‑100 (MQ=255)
                                                                   cccGTCTGCGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagt                          >  2:2489891/1‑100 (MQ=255)
                                                                          gggATTCTCTCCTCTCGAAAGCGTTTTTTTTTTTTTTTTTTTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGAAGCCGGGAGTAGTTtgt                   >  2:1764292/1‑100 (MQ=255)
                                                                           ggATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                  >  1:258974/1‑100 (MQ=255)
                                                                           ggATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                  <  2:2092015/100‑1 (MQ=255)
                                                                           ggATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                  <  1:1328227/100‑1 (MQ=255)
                                                                           ggATTCTCTCCTCGCGAAAGCGTTTTTTCTCATGAATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                  <  2:1761787/100‑1 (MQ=255)
                                                                            gATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                 >  2:1906897/1‑100 (MQ=255)
                                                                            gATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                 <  2:148353/100‑1 (MQ=255)
                                                                            gATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                 >  2:1906180/1‑100 (MQ=255)
                                                                               tctctcCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAtt              <  1:1071302/100‑1 (MQ=255)
                                                                                 tctcctcccGAAAGCGTTTTTTCTCCTGCATCATGTATGCAGGCGCGAGCCACTTCGTCATTACCGCAATCCTCGCAGCCGGGCGTCGTATGCGCATtgc            >  2:725408/1‑100 (MQ=255)
                                                                                    cctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc         <  2:1483881/100‑1 (MQ=255)
                                                                                    cctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc         >  1:1317913/1‑100 (MQ=255)
                                                                                    cctctcGAAAGCGATTTTTCTCCTGTATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc         <  2:2200128/100‑1 (MQ=255)
                                                                                     ctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCa        <  1:381809/100‑1 (MQ=255)
                                                                                      tctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc       <  2:1206860/100‑1 (MQ=255)
                                                                                       ctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACt      >  2:370753/1‑100 (MQ=255)
                                                                                       ctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACt      >  2:1720145/1‑100 (MQ=255)
                                                                                       ctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACt      >  1:1786829/1‑100 (MQ=255)
                                                                                        tcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg     <  2:1312797/100‑1 (MQ=255)
                                                                                        tcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg     >  1:1103997/1‑100 (MQ=255)
                                                                                          gAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAc   <  1:157307/100‑1 (MQ=255)
                                                                                           aaaGCGTTTTTTCTTTTGTTTCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAcc  >  2:1101289/1‑100 (MQ=255)
                                                                                           aaaGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAcc  <  1:961906/100‑1 (MQ=255)
                                                                                                 |                                                                                              
TGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACC  >  NC_000913/2809671‑2809862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: