Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 367,852 (GC)6→5 coding (568‑569/834 nt) mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913367,8420G.97.6% 385.1 / 2.0 84coding (579/834 nt)mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (45/37);  total (46/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01
*NC_000913367,8430C.97.6% 385.3 / 2.0 84coding (578/834 nt)mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base . (45/37);  total (46/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

AGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAAT  >  NC_000913/367745‑367932
                                                                                                 ||                                                                                         
aGCCAGTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcg                                                                                          <  2:685745/100‑1 (MQ=255)
aGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcg                                                                                          <  2:1292137/100‑1 (MQ=255)
 gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTACCCCTCTTTgcgc                                                                                         >  1:462863/1‑100 (MQ=255)
 gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgc                                                                                         >  2:554001/1‑100 (MQ=255)
 gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgc                                                                                         >  1:1737288/1‑100 (MQ=255)
  ccAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcg                                                                                        <  2:1724815/100‑1 (MQ=255)
  ccAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcg                                                                                        >  1:807225/1‑100 (MQ=255)
    aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcg                                                                                      <  2:2581349/100‑1 (MQ=255)
    aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcg                                                                                      >  2:1772250/1‑100 (MQ=255)
      tCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcg                                                                                    <  1:1659573/100‑1 (MQ=255)
       cACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgc                                                                                   >  1:2170679/1‑100 (MQ=255)
        aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgcc                                                                                  <  1:2013305/100‑2 (MQ=255)
         cccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgcca                                                                                 >  1:675976/1‑98 (MQ=255)
              tgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGaa                                                                            >  2:2292062/1‑100 (MQ=255)
               gttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATgg                                                                         <  1:1554682/100‑1 (MQ=255)
               gttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTT‑‑TCGCGCGCGCCAGAATgg                                                                         >  1:1031026/1‑100 (MQ=255)
                ttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGc                                                                        <  1:1140556/100‑1 (MQ=255)
                  cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCtt                                                                      >  2:754215/1‑100 (MQ=255)
                  cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCtt                                                                      >  2:673650/1‑100 (MQ=255)
                  cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCtt                                                                      >  2:306977/1‑100 (MQ=255)
                  cACTGCGCAGCGGTACGGCGATAGAGGCGAGCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCtt                                                                      >  2:196048/1‑100 (MQ=255)
                   aCTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTc                                                                     >  1:1320845/1‑100 (MQ=255)
                    cTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTcc                                                                    <  1:2075952/100‑1 (MQ=255)
                     tGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCa                                                                   <  1:1233202/100‑1 (MQ=255)
                     tGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCa                                                                   >  2:2184665/1‑100 (MQ=255)
                       cgcAGCGGTACGGCGATAGGGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGc                                                                 >  1:1821129/1‑100 (MQ=255)
                       cgcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGc                                                                 <  1:2476954/100‑1 (MQ=255)
                         cAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCtt                                                               >  1:867443/1‑100 (MQ=255)
                             ggTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                           <  1:1874757/100‑1 (MQ=255)
                             ggTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                           >  1:2130404/1‑100 (MQ=255)
                             ggTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                           <  2:1427177/100‑1 (MQ=255)
                              gTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACg                                                          >  1:1354884/1‑100 (MQ=255)
                              gTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACg                                                          >  1:2504586/1‑100 (MQ=255)
                              gTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACg                                                          >  1:1176598/1‑100 (MQ=255)
                                    cGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTccc                                                    <  1:2642748/100‑1 (MQ=255)
                                         ggggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                               <  2:1796815/98‑1 (MQ=255)
                                         gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                               >  2:2109747/1‑100 (MQ=255)
                                         gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                               <  2:1036411/100‑1 (MQ=255)
                                         gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                               <  1:335714/100‑1 (MQ=255)
                                         gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                               <  1:2557499/100‑1 (MQ=255)
                                         gagGCGAGCTTCCCCTCCGGACCCCAGGCGGGGTAGTGCTGGCCGTATCCCTCGGTGCGCGCGCGGCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                               <  2:1700473/100‑1 (MQ=255)
                                         gagGCGACCTTCTCCTCCTGCTCCCAGCCGCGGTCGTTCTGTCCGTAACCCTCCTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTCAACGGTTCCCCTGcc                                               >  2:1794842/1‑100 (MQ=255)
                                          agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCa                                              <  1:1790033/100‑1 (MQ=255)
                                           ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                             <  1:288575/100‑1 (MQ=255)
                                           ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                             <  2:1718124/100‑1 (MQ=255)
                                           ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                             >  2:2566379/1‑100 (MQ=255)
                                           ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                             >  2:1050834/1‑100 (MQ=255)
                                             cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                           >  2:1821325/1‑100 (MQ=255)
                                             cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                           >  2:923842/1‑100 (MQ=255)
                                             cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                           >  1:720440/1‑100 (MQ=255)
                                             cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                           >  1:1842887/1‑100 (MQ=255)
                                              gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTg                                          >  2:884326/1‑100 (MQ=255)
                                              gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTg                                          >  1:2294591/1‑100 (MQ=255)
                                                tcttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGAt                                        <  1:228807/100‑1 (MQ=255)
                                                 cttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                       <  1:1543999/100‑1 (MQ=255)
                                                 cttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                       <  2:793931/100‑1 (MQ=255)
                                                 cttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                       >  1:489197/1‑100 (MQ=255)
                                                 cttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                       >  1:1604438/1‑100 (MQ=255)
                                                  ttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAg                                      <  2:2389204/100‑1 (MQ=255)
                                                    ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACTCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtc                                    >  2:2221053/1‑100 (MQ=255)
                                                     tccttcCGATCCCAGCCGCGGTAGTTCTATCAGTCTCCCTCTTT‑‑‑‑GCGCGCGCCAGAATGGCGTCCCGCTTTAACGGTTCAGGTGCGCGGTGATCGtcatc                                 >  2:419678/1‑100 (MQ=255)
                                                     tcctccTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtca                                   <  1:2350183/100‑1 (MQ=255)
                                                       ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                                 >  1:1357189/1‑100 (MQ=255)
                                                       ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                                 <  1:2581198/100‑1 (MQ=255)
                                                       ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                                 >  1:1511435/1‑100 (MQ=255)
                                                        tcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatca                                >  1:1115637/1‑100 (MQ=255)
                                                        tcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatca                                <  1:565570/100‑1 (MQ=255)
                                                         cctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAc                               >  1:1041161/1‑100 (MQ=255)
                                                          ctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAcc                              >  2:2491474/1‑100 (MQ=255)
                                                             aTCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCggg                           >  2:2119061/1‑100 (MQ=255)
                                                              tCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCgggg                          <  1:1276820/100‑1 (MQ=255)
                                                              tCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCgggg                          >  2:454292/1‑100 (MQ=255)
                                                              tCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCgggg                          <  1:925196/100‑1 (MQ=255)
                                                              tCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCgggg                          >  2:1710025/1‑100 (MQ=255)
                                                                ccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                        >  1:1990500/1‑100 (MQ=255)
                                                                 cAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                       <  2:1337881/100‑1 (MQ=255)
                                                                  aGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCggg                      <  1:1304938/100‑1 (MQ=255)
                                                                    ccGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg                    <  2:188675/100‑1 (MQ=255)
                                                                         gTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTaa               >  1:1013351/1‑100 (MQ=255)
                                                                           aGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACa             >  1:915248/1‑100 (MQ=255)
                                                                            gTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAt            <  1:323525/100‑1 (MQ=255)
                                                                                tGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCTGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCg        <  1:2402126/100‑1 (MQ=255)
                                                                                tGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCg        >  2:410820/1‑100 (MQ=255)
                                                                                tGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCg        >  2:571388/1‑100 (MQ=255)
                                                                                 gTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGa       <  1:1080704/100‑1 (MQ=255)
                                                                                 gTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGa       <  1:1382403/100‑1 (MQ=255)
                                                                                  tCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAt      <  2:1552285/100‑1 (MQ=255)
                                                                                   ccGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAtt     <  1:413600/100‑1 (MQ=255)
                                                                                   ccGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAtt     <  2:1640026/100‑1 (MQ=255)
                                                                                   ccGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAtt     >  2:606520/1‑100 (MQ=255)
                                                                                    cGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATGACCGGGGCGGGAGGCTAACATTTCGATTa    >  2:187231/1‑100 (MQ=255)
                                                                                    cGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTa    <  1:67518/100‑1 (MQ=255)
                                                                                     gTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTaa   >  2:1010781/1‑100 (MQ=255)
                                                                                     gTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTaa   >  1:2222273/1‑100 (MQ=255)
                                                                                      tAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCCTCACCGGGGCGGCAGGCTAACATTTCGATTAAt  >  2:275549/1‑100 (MQ=255)
                                                                                      tAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAAt  <  1:1196523/100‑1 (MQ=255)
                                                                                      tAACCCTCTTT‑‑GCGCGAGCGCCAGACTTGCCTCCAGCTTTAACGGTGCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCAAACATTTCGATTAAt  <  2:1561653/100‑1 (MQ=255)
                                                                                                 ||                                                                                         
AGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAAT  >  NC_000913/367745‑367932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: