Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,216,0390CA45.6% 43.8 / 138.9 103L188M (CTG→ATG) aceBmalate synthase A
Reads supporting (aligned to +/- strand):  ref base C (24/32);  new base A (27/20);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 105 total aligned reads displayed.

CAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAGTCCTGGCAGG  >  NC_000913/4215940‑4216138
                                                                                                   |                                                                                                   
cAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGcc                                                                                                     <  1:511457/100‑1 (MQ=255)
  gCTCAAGCCCAATCCAGCGGTTTTGCTTTCTCGGGTACGCGGTCTGCACTTGCTGGAATAACTTGTCAACTGACGTGGTGAGGCACTCGCCCTCCGCCTg                                                                                                   <  2:1814713/100‑1 (MQ=255)
     cAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGttt                                                                                                >  1:1573050/1‑100 (MQ=255)
     cAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGttt                                                                                                >  1:2211546/1‑100 (MQ=255)
      aaGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTg                                                                                               <  1:1916909/100‑1 (MQ=255)
        gCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGAt                                                                                             >  1:139075/1‑100 (MQ=255)
          ccAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGAttt                                                                                           <  1:2635028/100‑1 (MQ=255)
          ccAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGAttt                                                                                           <  1:2232805/100‑1 (MQ=255)
            aaTCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTg                                                                                         >  2:600527/1‑100 (MQ=255)
            aaTCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTg                                                                                         <  1:1622499/100‑1 (MQ=255)
            aaTCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTg                                                                                         <  1:2455276/100‑1 (MQ=255)
            aaTACAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTg                                                                                         <  1:299566/100‑1 (MQ=255)
             aTCGAGCGGTTTTGATTTGTCGGGTAGGCGGTCTGCACTGGCAGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTgc                                                                                        <  1:2542401/100‑1 (MQ=255)
              tCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTgcg                                                                                       <  1:2274574/100‑1 (MQ=255)
               ccAGCGGATTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCCGTTTGATTTTgcgc                                                                                      >  1:1255250/1‑100 (MQ=255)
                cAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGct                                                                                     >  2:596220/1‑100 (MQ=255)
                 aGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGctc                                                                                    >  2:1890230/1‑100 (MQ=255)
                 aGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGctc                                                                                    <  2:1840011/100‑1 (MQ=255)
                    ggTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTAt                                                                                 <  1:1880237/100‑1 (MQ=255)
                     gTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTAtt                                                                                <  2:1999712/100‑1 (MQ=255)
                     gTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTAtt                                                                                >  1:1876470/1‑100 (MQ=255)
                       tttGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATttc                                                                              >  2:1567607/1‑100 (MQ=255)
                       tttGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATttc                                                                              <  2:73210/100‑1 (MQ=255)
                         tGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATttctt                                                                            <  1:1720249/100‑1 (MQ=255)
                         tGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATttctt                                                                            <  2:1973833/100‑1 (MQ=255)
                         tGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCAGGCAGCCTGTTTGATTTTGCGCTCTATttctt                                                                            >  1:888838/1‑100 (MQ=255)
                           aTTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTcc                                                                          <  2:1379445/100‑1 (MQ=255)
                           aTTTGGCGGCTACGCGGGCTGCACGTGCCGGAAAAACATGTCTCCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTcc                                                                          <  2:321423/100‑1 (MQ=255)
                            tttGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCca                                                                         <  1:1898627/100‑1 (MQ=255)
                             ttGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCcac                                                                        <  2:340313/100‑1 (MQ=255)
                             ttGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCcac                                                                        <  2:1649756/100‑1 (MQ=255)
                              tGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCcaca                                                                       <  1:1122605/100‑1 (MQ=255)
                              tGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCcaca                                                                       >  2:733623/1‑100 (MQ=255)
                               gTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACaa                                                                      >  1:1873473/1‑100 (MQ=255)
                               gTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACaa                                                                      >  1:114436/1‑100 (MQ=255)
                                tCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAAc                                                                     <  2:727943/100‑1 (MQ=255)
                                 cGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCGCCTGGCGTGGCGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACt                                                                    >  1:2359630/1‑100 (MQ=255)
                                 cGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACt                                                                    >  1:1348939/1‑100 (MQ=255)
                                  gggCACGCGGACTGCCCTTGCCGGACAAACAGGGCAACTGACTTCGTGAGGCAACCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCCGAACTa                                                                   <  2:1485911/100‑1 (MQ=255)
                                    gTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATc                                                                 <  1:1567802/100‑1 (MQ=255)
                                    gTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATc                                                                 >  2:82887/1‑100 (MQ=255)
                                       cgcgGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAgg                                                              >  2:846828/1‑100 (MQ=255)
                                            tCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTg                                                         >  2:1986399/1‑100 (MQ=255)
                                             cTGCACTTGCCGGAAAAACCTGTCACCTGGCGTGGTGAGACTATCCCCGGCAGCATGGTTGATGTTGCGCTAAATTTCTTCCACAACTATCAGGCACTGt                                                        >  2:2339586/1‑100 (MQ=255)
                                             cTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGt                                                        >  1:34772/1‑100 (MQ=255)
                                             cTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGt                                                        >  2:600897/1‑100 (MQ=255)
                                             cTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGt                                                        >  1:1068648/1‑100 (MQ=255)
                                              tGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGtt                                                       >  1:998814/1‑100 (MQ=255)
                                               gCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTg                                                      <  2:1353156/100‑1 (MQ=255)
                                                 aCTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGc                                                    <  1:1899333/100‑1 (MQ=255)
                                                 aCTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGc                                                    >  2:1600866/1‑100 (MQ=255)
                                                  cTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCa                                                   <  1:612883/100‑1 (MQ=255)
                                                   ttGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCaa                                                  <  2:1971900/100‑1 (MQ=255)
                                                    tGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCaaa                                                 >  1:544160/1‑100 (MQ=255)
                                                     gCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAg                                                <  2:2029143/100‑1 (MQ=255)
                                                     gCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAg                                                >  1:1205850/1‑100 (MQ=255)
                                                        tgAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGc                                             <  1:2142179/99‑1 (MQ=255)
                                                        ggAAGAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTGGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCACTGGGc                                             >  2:1766191/1‑100 (MQ=255)
                                                        ggAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGc                                             <  2:1766564/100‑1 (MQ=255)
                                                        ggAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGc                                             >  1:969713/1‑100 (MQ=255)
                                                        ggAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGc                                             <  1:2002767/100‑1 (MQ=255)
                                                         gAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCa                                            >  2:2096508/1‑100 (MQ=255)
                                                           aaaaCATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGt                                          <  2:2451587/100‑1 (MQ=255)
                                                           aaaaCATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGt                                          <  2:2451578/100‑1 (MQ=255)
                                                             aaCATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTgg                                        <  1:2262129/100‑1 (MQ=255)
                                                             aaCATGTCACCTGGCGTGGTGAGGCAACCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCCCAACTATCAGGCACTGTTGGCAAAGGGCAGTgg                                        <  2:644090/100‑1 (MQ=255)
                                                              aCATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGt                                       >  1:1317061/1‑100 (MQ=255)
                                                              aCATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGt                                       >  1:969692/1‑100 (MQ=255)
                                                                aTGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTcc                                     >  1:2328436/1‑100 (MQ=255)
                                                                 tGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTCTCAGGCACTGTTGGCAACGGGCAGTGGTccc                                    >  2:2037659/1‑100 (MQ=255)
                                                                 tGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTccc                                    <  2:683409/100‑1 (MQ=255)
                                                                   tCACCGGGCGAGGTGAGGCAATCCCCGGCAGCATGTTTGATTATGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTa                                  >  2:2212851/1‑100 (MQ=255)
                                                                     aCCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTAtt                                <  1:282564/100‑1 (MQ=255)
                                                                      ccTGGCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTAttt                               >  2:132479/1‑100 (MQ=255)
                                                                       cTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTc                              <  2:2568856/100‑1 (MQ=255)
                                                                         ggCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTa                            <  1:1712908/100‑1 (MQ=255)
                                                                         ggCGTGGTGAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTa                            >  2:1646200/1‑100 (MQ=255)
                                                                            gtggtgAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCt                         <  1:426702/100‑1 (MQ=255)
                                                                            gtggtgAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCt                         >  2:72488/1‑100 (MQ=255)
                                                                            gtggtgAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCt                         >  1:721333/1‑100 (MQ=255)
                                                                             tggtgAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTg                        <  1:1759528/100‑1 (MQ=255)
                                                                               gtgAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGcc                      <  1:2022543/100‑1 (MQ=255)
                                                                               gtgAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGcc                      <  1:1680166/100‑1 (MQ=255)
                                                                                tgAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCg                     <  1:1619704/100‑1 (MQ=255)
                                                                                 gAGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGa                    <  2:562889/100‑1 (MQ=255)
                                                                                  aGGCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGaa                   <  2:1752827/100‑1 (MQ=255)
                                                                                   ngCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGaaa                  >  1:2106081/2‑100 (MQ=255)
                                                                                   ggCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGaaa                  >  1:289033/1‑100 (MQ=255)
                                                                                   ggCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGaaa                  >  2:2469253/1‑100 (MQ=255)
                                                                                   ggCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGaaa                  >  1:1613661/1‑100 (MQ=255)
                                                                                   ggCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGaaa                  >  1:2488588/1‑100 (MQ=255)
                                                                                   ggCAATCCCCGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGaaa                  >  1:262808/1‑100 (MQ=255)
                                                                                       aTCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAAccc              <  1:1114584/100‑1 (MQ=255)
                                                                                         ccccGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAg            >  2:720017/1‑100 (MQ=255)
                                                                                          cccGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAGt           >  1:1692364/1‑100 (MQ=255)
                                                                                            cGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAGGcc         <  1:765386/100‑1 (MQ=255)
                                                                                            cGGCAGCCTGTTTGATTTTGCGCTCTATTGCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAGTcc         <  2:34228/100‑1 (MQ=255)
                                                                                            cGGCAGCATGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAGTcc         >  1:1111764/1‑100 (MQ=255)
                                                                                               cAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAGTCCTgg      <  1:1080397/100‑1 (MQ=255)
                                                                                                   cTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAGTCCTGGCAgg  >  2:1096029/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAGTCCTGGCAGG  >  NC_000913/4215940‑4216138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑