Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 826,009 C→G V34L (GTG→CTG)  ybhR ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913826,0090CG98.1% 375.1 / ‑4.0 107V34L (GTG→CTG) ybhRputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (54/51);  minor base T (1/1);  total (55/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TGGGTCAGCTCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATG  >  NC_000913/825912‑826102
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tGGGTCAGCTCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCt                                                                                             <  1:1283871/100‑1 (MQ=255)
  ggTCAGCTCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGa                                                                                           >  1:1200872/1‑100 (MQ=255)
     cAGCTCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAAtt                                                                                        <  1:1532315/100‑1 (MQ=255)
      aGCTCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTa                                                                                       <  2:210521/100‑1 (MQ=255)
      aGCTCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTa                                                                                       <  2:358331/100‑1 (MQ=255)
       gCTCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAg                                                                                      <  1:497260/100‑1 (MQ=255)
         tCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCa                                                                                    <  1:679299/100‑1 (MQ=255)
         tCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCa                                                                                    >  2:1005334/1‑100 (MQ=255)
          ccACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCAc                                                                                   >  1:2026257/1‑100 (MQ=255)
          ccACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCAc                                                                                   >  1:1998189/1‑100 (MQ=255)
          ccACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCAc                                                                                   >  1:2025435/1‑100 (MQ=255)
            aCCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACgg                                                                                 >  2:482907/1‑100 (MQ=255)
            aCCGAATGCTCGACGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGAGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACgg                                                                                 >  2:2495569/1‑100 (MQ=255)
             ccGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACggg                                                                                <  1:1414894/100‑1 (MQ=255)
             ccGAATGCTCGCCGTCATCTTCATCGTGGATGGTGTTGGTGGCGTTAGTCACTGCCAGCGTGGCGGAGGACGGGAACAGGAGCATCTGAATTAGCACggg                                                                                <  2:2335189/100‑1 (MQ=255)
                aaTGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTaa                                                                             >  1:1480013/1‑100 (MQ=255)
                   gCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAAt                                                                          >  1:1676477/1‑100 (MQ=255)
                   gCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAAt                                                                          >  1:195511/1‑100 (MQ=255)
                    cTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATc                                                                         >  1:1527374/1‑100 (MQ=255)
                    cTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATc                                                                         >  2:1805858/1‑100 (MQ=255)
                     tCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCa                                                                        <  2:1513888/100‑1 (MQ=255)
                      cGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAg                                                                       <  2:1109874/100‑1 (MQ=255)
                          gTTATCTTCATCGTAGATGGCGATGGTGGTGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCATCTGAATTAGCACGGGTAAAATCAGAATc                                                                   >  1:931810/1‑100 (MQ=255)
                          gTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATc                                                                   >  2:425851/1‑100 (MQ=255)
                           ttATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATcg                                                                  <  2:200363/100‑1 (MQ=255)
                           ttATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATcg                                                                  >  2:2193256/1‑100 (MQ=255)
                              tCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATcgcgc                                                               <  1:1837769/100‑1 (MQ=255)
                              tCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATcgcgc                                                               <  1:115632/100‑1 (MQ=255)
                               cTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATcgcgcg                                                              >  2:1241096/1‑100 (MQ=255)
                                ttCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGCACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCgg                                                             >  2:1109889/1‑100 (MQ=255)
                                ttCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCgg                                                             >  1:763998/1‑100 (MQ=255)
                                 tCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCggg                                                            <  2:2139408/100‑1 (MQ=255)
                                 tCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCggg                                                            <  2:36448/100‑1 (MQ=255)
                                  cagcGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGt                                                           <  2:771228/97‑1 (MQ=255)
                                  cATCGTAGATTGCGATGATGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCCCGGGTAAAATCAGAATCGCGCGGGt                                                           >  2:2040269/1‑100 (MQ=255)
                                   aTCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGtt                                                          <  2:815963/100‑1 (MQ=255)
                                    tCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGttt                                                         >  1:1798317/1‑100 (MQ=255)
                                     cGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTg                                                        <  1:1342111/100‑1 (MQ=255)
                                      gTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGc                                                       >  1:1306438/1‑100 (MQ=255)
                                        aGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCgg                                                     <  2:650629/100‑1 (MQ=255)
                                           tGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTc                                                  >  2:560287/1‑100 (MQ=255)
                                           tGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTc                                                  >  1:946088/1‑100 (MQ=255)
                                              cGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTcgcg                                               >  2:1877025/1‑100 (MQ=255)
                                              cGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTcgcg                                               >  1:382842/1‑100 (MQ=255)
                                               gATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCgcgc                                              <  2:1894588/100‑1 (MQ=255)
                                                acggtggCGTTAGTCACTTCCAGCGTGGCGCCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCgca                                             <  2:460691/98‑1 (MQ=255)
                                                 tggtggCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCgcag                                            <  2:1245056/100‑1 (MQ=255)
                                                 tggtggCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCgcag                                            <  2:2305664/100‑1 (MQ=255)
                                                  ggtggCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCgcagc                                           <  2:333608/100‑1 (MQ=255)
                                                   gtggCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCgcagca                                          <  1:1327288/100‑1 (MQ=255)
                                                    tggCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGATTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCaa                                         >  1:2562870/1‑100 (MQ=255)
                                                     ggCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAAc                                        >  1:2025691/1‑100 (MQ=255)
                                                     ggCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAAc                                        >  2:2259016/1‑100 (MQ=255)
                                                     ggCGGTAGTCACGTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAAc                                        <  2:636450/100‑1 (MQ=255)
                                                       cGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGa                                      >  2:1337860/1‑100 (MQ=255)
                                                       cGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAACCGCGCGGGTTTGCGGTTCGCGCAGCAACGa                                      >  2:2087307/1‑100 (MQ=255)
                                                        gTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCGCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGAc                                     >  1:46881/1‑100 (MQ=255)
                                                        gTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGAc                                     <  1:714069/100‑1 (MQ=255)
                                                        gTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGAc                                     >  1:60818/1‑100 (MQ=255)
                                                        gTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGAc                                     >  2:1689318/1‑100 (MQ=255)
                                                         ttAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACt                                    <  1:1450955/100‑1 (MQ=255)
                                                          tAGTCACTTCCAGGGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTg                                   >  2:1096429/1‑100 (MQ=255)
                                                          tAGTCACTTCCAGCGTGGCGGCGAACTGGAACAGGATCAGCTGAATTAGCACGGGCAAAATGAGAATCACGCGGGTTTGCGGTTCGCGCATCAACGGCTg                                   <  2:1943502/100‑1 (MQ=255)
                                                           aGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCCGCAACGACTGc                                  >  2:835035/1‑100 (MQ=255)
                                                            gTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCa                                 <  2:1802130/100‑1 (MQ=255)
                                                            gTCACTTCCAGCGTGCCGGCGAACGGGAACAGGATCAGCTAAATTGGCACGGGTAAAGTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCa                                 >  1:2296248/1‑100 (MQ=255)
                                                              cACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAAc                               >  1:2231863/1‑100 (MQ=255)
                                                              cACTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGCTCGCGCAGCAACGACTGCAAc                               >  2:1167888/1‑100 (MQ=255)
                                                                cTTCCAGCGTGGCGGCGAACGGGAACAGTATCAGCTGAATAAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAActc                             <  2:310242/100‑1 (MQ=255)
                                                                cTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAActc                             >  2:617705/1‑100 (MQ=255)
                                                                cTTCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTCAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAActc                             >  2:2432071/1‑100 (MQ=255)
                                                                  tCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCtt                           <  2:1144618/100‑1 (MQ=255)
                                                                  tCCAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCttt                          >  1:806592/1‑100 (MQ=255)
                                                                   ccAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCttt                          >  1:318378/1‑100 (MQ=255)
                                                                    cAGCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTg                         <  1:2083896/100‑1 (MQ=255)
                                                                      gCGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCg                       <  1:540649/100‑1 (MQ=255)
                                                                       cGTGGCGGCGAACGGGAACAGGATCAGGTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCgg                      <  2:1101512/100‑1 (MQ=255)
                                                                       cGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCgg                      >  1:105067/1‑100 (MQ=255)
                                                                       cGTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCgg                      <  2:2346470/100‑1 (MQ=255)
                                                                        gTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGa                     <  2:1136259/100‑1 (MQ=255)
                                                                        gTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGa                     >  2:59427/1‑100 (MQ=255)
                                                                        gTGGCGGCGAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTGGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGa                     <  2:741851/100‑1 (MQ=255)
                                                                              gcgAACGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACg               <  1:505876/100‑1 (MQ=255)
                                                                                 aaCGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTcc            >  2:1658089/1‑100 (MQ=255)
                                                                                 aaCGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTcc            <  2:326444/100‑1 (MQ=255)
                                                                                 aaCGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTcc            <  1:1550677/100‑1 (MQ=255)
                                                                                 aaCGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTcc            <  1:1994884/100‑1 (MQ=255)
                                                                                  aCGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCa           <  1:1465824/100‑1 (MQ=255)
                                                                                  aCGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCa           >  2:712480/1‑100 (MQ=255)
                                                                                   cGGGAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCAt          <  2:1181033/100‑1 (MQ=255)
                                                                                    gggAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATa         >  2:846026/1‑100 (MQ=255)
                                                                                     ggAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATaa        >  2:2525595/1‑100 (MQ=255)
                                                                                     ggAACAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATaa        >  1:560183/1‑100 (MQ=255)
                                                                                      gAACAGGATCAGCCGAATTAGCACGCGTAAAACCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAg       <  2:561842/100‑1 (MQ=255)
                                                                                        aCAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCg     >  1:919847/1‑100 (MQ=255)
                                                                                         cAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGa    >  1:720965/1‑100 (MQ=255)
                                                                                         cAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGa    <  1:2084095/100‑1 (MQ=255)
                                                                                         cAGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTGGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGa    >  1:782709/1‑100 (MQ=255)
                                                                                          aGGATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGAt   <  1:2615303/100‑1 (MQ=255)
                                                                                           ggATCAGCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAGCGTCCATAAGCGATg  >  2:416061/1‑100 (MQ=255)
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TGGGTCAGCTCCACCGAATGCTCGCCGTTATCTTCATCGTAGATGGCGATGGTGGCGTTAGTCACTTCCAGCGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATG  >  NC_000913/825912‑826102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: