Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 28,175 T→A W295R (TGG→AGG)  rihC → ribonucleoside hydrolase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091328,1750TA98.0% 342.1 / ‑1.9 100W295R (TGG→AGG) rihCribonucleoside hydrolase 3
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (53/45);  total (54/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

Only 100 of 105 total aligned reads displayed.

TACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCC  >  NC_000913/28078‑28270
                                                                                                 |                                                                                               
tACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAgagg                                                                                               <  2:1054016/100‑1 (MQ=255)
  ccTCGGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAgagggt                                                                                             <  2:1567734/100‑1 (MQ=255)
  ccTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAgagggt                                                                                             >  2:1605657/1‑100 (MQ=255)
   cTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGAGCTGGATGTGAAAGGCTTCCAGCAgtgggtg                                                                                            >  1:1496369/1‑100 (MQ=255)
      aGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCt                                                                                         <  1:1886241/100‑1 (MQ=255)
       ggCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAgagggtggagg                                                                                        >  2:2364188/1‑97 (MQ=255)
       ggCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTg                                                                                        >  2:566790/1‑100 (MQ=255)
       ggCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTg                                                                                        >  1:786010/1‑100 (MQ=255)
        gCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGa                                                                                       <  2:1058555/100‑1 (MQ=255)
        gCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGa                                                                                       <  1:718152/100‑1 (MQ=255)
         cACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAg                                                                                      >  2:864813/1‑100 (MQ=255)
             acgGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAgatcg                                                                                  >  1:172753/1‑96 (MQ=255)
              cgGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCt                                                                                 >  2:1638462/1‑100 (MQ=255)
                  ggtTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGct                                                                             <  1:1774289/100‑1 (MQ=255)
                   gtTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGctc                                                                            >  1:1830933/1‑100 (MQ=255)
                    ttGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGctct                                                                           >  1:923956/1‑100 (MQ=255)
                      gATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTgg                                                                         >  2:1045050/1‑100 (MQ=255)
                      gATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTgg                                                                         <  1:38631/100‑1 (MQ=255)
                      gATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTgg                                                                         <  1:1623058/100‑1 (MQ=255)
                       atatCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGc                                                                        <  1:1439739/100‑1 (MQ=255)
                       atatCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGc                                                                        <  1:715499/100‑1 (MQ=255)
                        tatCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGcg                                                                       >  1:1100700/1‑100 (MQ=255)
                        tatCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGcg                                                                       >  1:200146/1‑100 (MQ=255)
                          tCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGcgtc                                                                     <  1:229126/100‑1 (MQ=255)
                          tCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGcgtc                                                                     <  1:218575/100‑1 (MQ=255)
                           cGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGcgtcg                                                                    <  2:1266436/100‑1 (MQ=255)
                               ggTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAAc                                                                >  2:1455095/1‑100 (MQ=255)
                               ggTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAAc                                                                <  2:899377/100‑1 (MQ=255)
                                  tGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTg                                                             >  1:1406516/1‑100 (MQ=255)
                                  tGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTg                                                             >  2:2399442/1‑100 (MQ=255)
                                   gCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGt                                                            >  2:917736/1‑100 (MQ=255)
                                   gCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGt                                                            >  1:1171204/1‑100 (MQ=255)
                                      tGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTcac                                                         >  1:2132763/1‑100 (MQ=255)
                                      tGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTcac                                                         >  1:2302888/1‑100 (MQ=255)
                                          cAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGt                                                     <  2:137158/100‑1 (MQ=255)
                                          cAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGt                                                     >  2:772510/1‑100 (MQ=255)
                                          cAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGt                                                     <  1:1290797/100‑1 (MQ=255)
                                           cgcccagccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGtt                                                    >  2:1204986/4‑100 (MQ=255)
                                           aaGCCAGCCAATGTACAGGCGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGtt                                                    <  2:1853123/100‑1 (MQ=255)
                                            agccagccAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTa                                                   >  2:1678720/1‑100 (MQ=255)
                                             gccagccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTAt                                                  >  1:2320108/1‑100 (MQ=255)
                                             gccagccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTAt                                                  >  2:1006618/1‑100 (MQ=255)
                                             gccagccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGGTAt                                                  >  1:1234894/1‑100 (MQ=255)
                                               cagccaGTGTACAGGTGGCATTGGATCTGGATGTGAACGGCTTCTTGCAGAGGGTGGCTGACGTGCTGGACCTCGCGGCGTGACCCGTCACATGTTATTg                                                >  2:2512091/1‑100 (MQ=255)
                                               cagccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTg                                                >  2:2546061/1‑100 (MQ=255)
                                               cagccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTg                                                >  2:2546127/1‑100 (MQ=255)
                                                agccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTgg                                               <  2:1595088/100‑1 (MQ=255)
                                                agccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTgg                                               >  2:1034042/1‑100 (MQ=255)
                                                agccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTgg                                               <  2:585578/100‑1 (MQ=255)
                                                agccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTgg                                               <  2:1833443/100‑1 (MQ=255)
                                                  ccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCa                                             <  2:908601/100‑1 (MQ=255)
                                                  ccaATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCa                                             <  2:1637201/100‑1 (MQ=255)
                                                   caATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGTTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCAt                                            >  2:2513608/1‑100 (MQ=255)
                                                    aaTGTACAGGTGGCATCGGATCTGGATGTGAACGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATg                                           <  1:121204/100‑1 (MQ=255)
                                                     aTGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGc                                          >  2:1510932/1‑100 (MQ=255)
                                                      tGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCa                                         >  1:822963/1‑100 (MQ=255)
                                                      tGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCa                                         >  1:2612571/1‑100 (MQ=255)
                                                       gTACAGGTGGGGTTGGATGTGGATGTGAAAGGCTTCCAGCAGAGGGTGGGTGAGGTGCTGGGTCTGGCGTCGTAACCTGTCGCATGTTATTGGCATGCAg                                        <  2:1589038/100‑1 (MQ=255)
                                                       gTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAg                                        <  2:2331279/100‑1 (MQ=255)
                                                       gTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAg                                        >  2:1274615/1‑100 (MQ=255)
                                                        tACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGt                                       <  2:137713/100‑1 (MQ=255)
                                                         aCAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGtc                                      <  2:749529/100‑1 (MQ=255)
                                                          cAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGtca                                     <  1:1765890/100‑1 (MQ=255)
                                                          cAGGTGGCATTGGATCTGGATGCGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGtca                                     <  2:1475753/100‑1 (MQ=255)
                                                            tgTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGtcatt                                   <  2:1971559/99‑1 (MQ=255)
                                                            ggTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGtcatt                                   >  1:935621/1‑100 (MQ=255)
                                                            ggTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGCCACATGTTATTGGCATGCAGtcatt                                   >  2:956604/1‑100 (MQ=255)
                                                             gTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGtcattc                                  >  1:2067790/1‑100 (MQ=255)
                                                             gTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGtcatta                                  >  1:1997762/1‑99 (MQ=255)
                                                              tGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAAGTGCTTTCTCTTTTTTCTTAACCGGTCACATCTTATTGGCATGCAGtcattca                                 <  2:1752558/100‑1 (MQ=255)
                                                                gCATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATc                               <  1:1175030/100‑1 (MQ=255)
                                                                 cATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCg                              <  1:1015414/100‑1 (MQ=255)
                                                                 cATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCg                              <  1:677171/100‑1 (MQ=255)
                                                                 cATTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCAGTCATCg                              <  2:1930715/100‑1 (MQ=255)
                                                                  aTTGGATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACATGTCACATGTTATTGGCATGCAGTCATTCATCGa                             >  1:1449692/1‑100 (MQ=255)
                                                                      gATCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCa                         <  1:2133551/100‑1 (MQ=255)
                                                                       aTCTGGATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCAt                        >  2:1761785/1‑100 (MQ=255)
                                                                           ggATGTGAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCt                    >  2:2540309/1‑100 (MQ=255)
                                                                           ggAGGTGAAAGGCATCCAGCAGAGGGTGGCCGAGGTCCTGGCCCTGGCGTCGTAACCTGTCACATGTCATTGGCACGCAGCCATTCAGCGCCTCATCCCt                    >  2:111332/1‑100 (MQ=255)
                                                                              tgtgAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTc                 <  1:1701788/100‑1 (MQ=255)
                                                                              tgtgAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTc                 >  1:2075979/1‑100 (MQ=255)
                                                                               gtgAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCAGTCACACGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCa                >  2:1308082/1‑100 (MQ=255)
                                                                                tgAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCAc               >  2:890357/1‑100 (MQ=255)
                                                                                tgAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCAc               >  2:1303362/1‑100 (MQ=255)
                                                                                 gAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACt              >  2:1138741/1‑100 (MQ=255)
                                                                                 gAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACt              <  1:1030211/100‑1 (MQ=255)
                                                                                 gAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACt              <  1:1072771/100‑1 (MQ=255)
                                                                                 gAAAGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACt              >  1:1119050/1‑100 (MQ=255)
                                                                                  tctggCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTg             <  2:2306712/97‑1 (MQ=255)
                                                                                   aaGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGa            <  2:1703268/100‑1 (MQ=255)
                                                                                    aGGCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGat           <  1:1300428/100‑1 (MQ=255)
                                                                                     ggCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGaga          >  1:2351504/1‑98 (MQ=255)
                                                                                      gCTTCCAGCAGAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGatat         <  2:303506/100‑1 (MQ=255)
                                                                                       cTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATAtc        >  2:121397/1‑100 (MQ=255)
                                                                                           cagcagAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATAtccctc    >  1:1637281/1‑100 (MQ=255)
                                                                                            agcagAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATAtccctcc   >  1:2237350/1‑100 (MQ=255)
                                                                                             gcagAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATAtccctccc  >  1:712391/1‑100 (MQ=255)
                                                                                             gcagAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATAtccctccc  <  1:1260334/100‑1 (MQ=255)
                                                                                             gcagAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATAtccctccc  <  1:1703428/100‑1 (MQ=255)
                                                                                             gcagAGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATAtccctccc  <  1:2041948/100‑1 (MQ=255)
                                                                                                 |                                                                                               
TACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCC  >  NC_000913/28078‑28270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: