Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,078,157 C→T P242P (CCG→CCA putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,078,1570CT99.2% 379.3 / ‑4.6 117P242P (CCG→CCAputAfused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (58/58);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

Only 100 of 117 total aligned reads displayed.

GCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAG  >  NC_000913/1078060‑1078253
                                                                                                 |                                                                                                
gCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTgg                                                                                                <  2:1268084/100‑1 (MQ=255)
 cTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGt                                                                                               <  2:1615025/100‑1 (MQ=255)
   tGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTc                                                                                             >  2:363449/1‑100 (MQ=255)
    gCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCa                                                                                            >  1:2438258/1‑100 (MQ=255)
     cGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCGGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCAc                                                                                           >  2:1221041/1‑100 (MQ=255)
     cGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCAc                                                                                           >  2:1272851/1‑100 (MQ=255)
      gggCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCAcc                                                                                          <  2:2142111/100‑1 (MQ=255)
      gggCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCAcc                                                                                          <  2:1541668/100‑1 (MQ=255)
        gCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGc                                                                                        <  1:2202437/100‑1 (MQ=255)
        gCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACACATAAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGc                                                                                        >  1:1861634/1‑100 (MQ=255)
         cATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCt                                                                                       <  2:2299832/100‑1 (MQ=255)
          aTTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCtt                                                                                      <  1:540248/100‑1 (MQ=255)
          aTTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCtt                                                                                      <  2:2453114/100‑1 (MQ=255)
           ttGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCttt                                                                                     <  2:1798325/100‑1 (MQ=255)
            tGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCtttt                                                                                    >  2:763386/1‑100 (MQ=255)
            tGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCtttt                                                                                    <  2:459579/100‑1 (MQ=255)
            tGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCtttt                                                                                    <  1:2038368/100‑1 (MQ=255)
            tGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGAGCAGTGGTTCACCGCtttt                                                                                    >  2:2397816/1‑100 (MQ=255)
              gCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTAc                                                                                  >  1:192180/1‑100 (MQ=255)
              gCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTAc                                                                                  >  1:192326/1‑100 (MQ=255)
               ctagcGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTAcc                                                                                 <  1:1562379/96‑1 (MQ=255)
               cTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTAcc                                                                                 >  2:1356233/1‑100 (MQ=255)
               cTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTAcc                                                                                 >  1:255190/1‑100 (MQ=255)
               cTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTAcc                                                                                 <  2:176725/100‑1 (MQ=255)
               cTAACGCGTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTAcc                                                                                 <  2:1214599/100‑1 (MQ=255)
                   cGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATa                                                                             <  1:1121928/100‑1 (MQ=255)
                     cTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAAt                                                                           >  1:890876/1‑100 (MQ=255)
                      ttCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATg                                                                          >  1:1774772/1‑100 (MQ=255)
                       tCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGc                                                                         <  1:1675600/100‑1 (MQ=255)
                        ccGCGATGGTTTCGCCAGTGACGAACTGCGCAGCCATCAGGGGCATCGCCATATCCACACCTTTGCGGATCATTGGTTCACCGCTTTTACCGATAATGCg                                                                        <  2:1498060/100‑1 (MQ=255)
                         cgcgATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCgg                                                                       <  1:1267498/100‑1 (MQ=255)
                         cgcgATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCgg                                                                       >  2:695626/1‑100 (MQ=255)
                          gcgATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGt                                                                      <  2:1137492/100‑1 (MQ=255)
                          gcgATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGt                                                                      >  2:2550881/1‑100 (MQ=255)
                            gATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTc                                                                    <  2:1655558/100‑1 (MQ=255)
                              tGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCag                                                                  <  1:1341586/100‑1 (MQ=255)
                              tGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCag                                                                  <  1:776460/100‑1 (MQ=255)
                               ggTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCagc                                                                 <  1:1679666/100‑1 (MQ=255)
                                gTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCagcg                                                                >  2:1353621/1‑100 (MQ=255)
                                gTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCagcg                                                                <  1:508796/100‑1 (MQ=255)
                                 tttCGCCAGTGACGAACTGCTCACCCATCAGACGCATAGCCATATCCACACCTTTGCGGCTCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCagcga                                                               >  1:969477/1‑100 (MQ=255)
                                    cGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCagcgagcg                                                            <  2:952634/100‑1 (MQ=255)
                                      ccAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCggg                                                          <  1:1492316/100‑1 (MQ=255)
                                           gACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAgg                                                     >  2:1724748/1‑100 (MQ=255)
                                             cGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggct                                                   >  1:783598/1‑100 (MQ=255)
                                             cGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggct                                                   >  2:2186138/1‑100 (MQ=255)
                                             cGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggct                                                   >  1:1979625/1‑100 (MQ=255)
                                              gAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggctg                                                  <  2:93136/100‑1 (MQ=255)
                                               aaCTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggctgg                                                 <  1:1913640/100‑1 (MQ=255)
                                               aaCTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggctgg                                                 >  2:100695/1‑100 (MQ=255)
                                               aaCTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggctgg                                                 >  1:2089707/1‑100 (MQ=255)
                                                aCTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggctggc                                                <  1:1627768/100‑1 (MQ=255)
                                                aCTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggctggc                                                >  2:424946/1‑100 (MQ=255)
                                                 cTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggctggct                                               >  2:1234571/1‑100 (MQ=255)
                                                 cTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAggctggct                                               <  1:115790/100‑1 (MQ=255)
                                                   gCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTc                                             >  1:2205162/1‑100 (MQ=255)
                                                   gCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTc                                             >  1:1848021/1‑100 (MQ=255)
                                                    cTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGCGAGGCTGGCCTCg                                            >  2:1355768/1‑100 (MQ=255)
                                                    cTCACCCATCAGGCGCATCGCCATATCCACACCTTCGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCg                                            <  2:2187924/100‑1 (MQ=255)
                                                     tCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGt                                           <  1:2165996/100‑1 (MQ=255)
                                                      cACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTGTTAGCGCTAATGCGGTGCGGCGGGGGGGAGAggcgggctggggg                                          >  2:13538/1‑95 (MQ=255)
                                                       aCCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTa                                         <  2:958693/100‑1 (MQ=255)
                                                       aCCCATCAGGCGCATCGCCATATCCACACCTTGGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTa                                         <  2:1982071/100‑1 (MQ=255)
                                                        cccATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTAt                                        >  2:1467088/1‑100 (MQ=255)
                                                        cccATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTAt                                        >  1:741080/1‑100 (MQ=255)
                                                         ccATCAGGCGCATCGCCATATCCACACCGTTGCGGATCAGTGGTTCACCGCTGTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTAtn                                       <  2:2154374/100‑2 (MQ=255)
                                                          cATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGATGCTGGATTCGTTATgg                                      >  1:1067233/1‑100 (MQ=255)
                                                          cATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATgg                                      >  1:2379355/1‑100 (MQ=255)
                                                           aTCAGGCGCATCGCCATAACCACAACTTTGCGGGTCAGTGGTTCACCGATGTTACAGATAATGCGGTTCCGCGAGCGGGAGAGGCTGGCTTCGTTATggg                                     <  2:1423133/100‑1 (MQ=255)
                                                              aGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTgg                                  <  1:754068/100‑1 (MQ=255)
                                                              aGGCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTgg                                  <  1:516109/100‑1 (MQ=255)
                                                               ggCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGa                                 >  2:168194/1‑100 (MQ=255)
                                                               ggCGCATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGa                                 >  1:978294/1‑100 (MQ=255)
                                                                gcgcATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGaa                                <  1:262166/100‑1 (MQ=255)
                                                                 cgcATCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGaaa                               <  2:2301463/100‑1 (MQ=255)
                                                                    aTCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCa                            >  1:2468352/1‑100 (MQ=255)
                                                                     tCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAg                           <  2:776112/100‑1 (MQ=255)
                                                                     tCGCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAg                           >  1:2609556/1‑100 (MQ=255)
                                                                       gCCATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTAGCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGtt                         >  1:2246832/1‑100 (MQ=255)
                                                                        ccATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGttt                        <  1:1886752/100‑1 (MQ=255)
                                                                         cATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTg                       >  2:1030811/1‑100 (MQ=255)
                                                                         cATATCCACACCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTg                       <  2:2381627/100‑1 (MQ=255)
                                                                               cacaCCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTa                 >  1:1130865/1‑100 (MQ=255)
                                                                               cacaCCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTa                 >  1:1130899/1‑100 (MQ=255)
                                                                               cacaCCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTa                 <  1:759221/100‑1 (MQ=255)
                                                                               cacaCCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAACGCGGTTCAGCGAGCGGGAGCGGCTGGCCTCGTTATGAGCGGAAACCAGTCTGTCAGTa                 >  2:1694233/1‑100 (MQ=255)
                                                                                 cacCTTTGCCGATCAGTAGTTCACCCCTTTTACCGATAATGCAGTTAAGCAAACGGGAGAGGCTGGCTTCGTTATGGGTCGAAACCACTTTGCCAGTaaa               <  2:1340821/100‑1 (MQ=255)
                                                                                  acCTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAAc              <  1:2213296/100‑1 (MQ=255)
                                                                                   ccTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAAca             >  1:99212/1‑100 (MQ=255)
                                                                                   ccTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAAca             >  1:1191687/1‑100 (MQ=255)
                                                                                    cTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAAcag            >  1:13702/1‑100 (MQ=255)
                                                                                    cTTTGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAAcag            <  1:1322143/100‑1 (MQ=255)
                                                                                     tttGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAAcagc           >  1:250417/1‑100 (MQ=255)
                                                                                      ttGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAAcagca          <  2:1593633/100‑1 (MQ=255)
                                                                                       tGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAAcagcag         >  2:532417/1‑100 (MQ=255)
                                                                                       tGCGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAAcagcag         >  2:580883/1‑100 (MQ=255)
                                                                                         cGGATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGcc       <  1:2503103/100‑1 (MQ=255)
                                                                                           gATCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGcccc     >  2:437487/1‑100 (MQ=255)
                                                                                             tCAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCa   <  2:2334292/100‑1 (MQ=255)
                                                                                              cAGTGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAg  >  1:53323/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAG  >  NC_000913/1078060‑1078253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: