Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,108,247	A→G	I232T (ATT→ACT)	opgC ←	OPG biosynthetic transmembrane succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,108,247	0	A	G	98.6%	232.3 / NA	70	I232T (ATT→ACT)	opgC	OPG biosynthetic transmembrane succinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (31/38);  minor base T (0/1);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATA  >  NC_000913/1108150‑1108341                                                                                                  |                                                                                               aTAGCGCTTGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTg                                                                                              <  2:1413921/100‑1 (MQ=255)    gcgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGaaa                                                                                           >  2:863482/1‑100 (MQ=255)    gcgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGaaa                                                                                           >  2:1845992/1‑100 (MQ=255)     cgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAg                                                                                          >  2:987773/1‑100 (MQ=255)     cgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAg                                                                                          <  2:1177698/100‑1 (MQ=255)     cgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAg                                                                                          <  1:1738039/100‑1 (MQ=255)      gcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGc                                                                                         >  1:86852/1‑100 (MQ=255)         ggTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAg                                                                                      >  1:404361/1‑100 (MQ=255)             gAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTgcg                                                                                  >  1:2435190/1‑100 (MQ=255)              aGTAAATAAGCGACAAACGCCAATGCTTCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGACTTTAAGATGAGGGAAAGTGAAAGACACTgcgc                                                                                 >  2:313128/1‑100 (MQ=255)              aGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTgcgc                                                                                 >  1:915966/1‑100 (MQ=255)              aGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTgcgc                                                                                 >  1:885488/1‑100 (MQ=255)               gTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGcc                                                                                <  1:855994/100‑1 (MQ=255)                tAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCg                                                                               >  2:2350197/1‑100 (MQ=255)                  aaTAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAg                                                                             <  2:2234657/100‑1 (MQ=255)                   aTAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAgg                                                                            >  2:242297/1‑100 (MQ=255)                   aTAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAgg                                                                            <  1:1396649/100‑1 (MQ=255)                    tAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGa                                                                           <  2:1476979/100‑1 (MQ=255)                     aaGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGAt                                                                          <  2:63155/100‑1 (MQ=255)                      aGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATa                                                                         >  1:1194538/1‑100 (MQ=255)                         gACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAg                                                                      <  2:79910/100‑1 (MQ=255)                          aCAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGAGAAAGa                                                                     <  2:1814210/100‑1 (MQ=255)                            aaaCGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAAc                                                                   <  2:154673/100‑1 (MQ=255)                             aaCGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACg                                                                  >  1:2617970/1‑100 (MQ=255)                             aaCGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACg                                                                  <  2:1871412/100‑1 (MQ=255)                              aCGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACgg                                                                 >  1:94938/1‑100 (MQ=255)                               cGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGTCAGTGCGCCGAGGATAAAGAACGGc                                                                <  2:297528/100‑1 (MQ=255)                                 ccAATGCTGGTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCaa                                                              <  2:1330100/100‑1 (MQ=255)                                 ccAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCaa                                                              <  2:2410396/100‑1 (MQ=255)                                 ccAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCaa                                                              <  1:2323276/100‑1 (MQ=255)                                        tgctgcAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAAc                                                       <  1:2223125/100‑1 (MQ=255)                                          ctgcAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAg                                                     >  1:573121/1‑100 (MQ=255)                                           tgcAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGGACGGCACATAAAACAGc                                                    >  2:12176/1‑100 (MQ=255)                                            gcAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCg                                                   >  1:2019442/1‑100 (MQ=255)                                               aGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGttt                                                >  1:989621/1‑100 (MQ=255)                                               aGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGttt                                                <  2:227563/100‑1 (MQ=255)                                                  gTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGCTGAGGGAAAGTGAACGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCa                                             <  1:1724036/100‑1 (MQ=255)                                                  gTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCa                                             >  1:363078/1‑100 (MQ=255)                                                   tACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCAt                                            <  1:1563172/100‑1 (MQ=255)                                                    aaaGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATg                                           <  2:2242004/98‑1 (MQ=255)                                                    aCAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATg                                           >  2:1079972/1‑100 (MQ=255)                                                    aCAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATg                                           <  2:1267844/100‑1 (MQ=255)                                                     gaGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGa                                          >  2:515387/2‑100 (MQ=255)                                                      aGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGAc                                         <  2:1176791/100‑1 (MQ=255)                                                              gaCGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAAt                                 >  1:2483205/1‑100 (MQ=255)                                                              gaCGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAAt                                 <  1:582601/100‑1 (MQ=255)                                                                   cGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAAc                            <  1:1362635/100‑1 (MQ=255)                                                                   cGTGGTAAACAAGGCTTTAAGAAGAGGGAAAGTCAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTACATGACAATAAAATTGaag                            >  2:264967/1‑99 (MQ=255)                                                                         aaaCAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCa                      >  1:585019/1‑100 (MQ=255)                                                                         aaaCAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCa                      >  1:37824/1‑100 (MQ=255)                                                                          aaCAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCAt                     <  1:765935/100‑1 (MQ=255)                                                                           ataaGGCTTTAAGATGAGGAAAAGTGAACGCCAGTGCGCCGAGGATAAAGAACGGCAATTAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCAtt                    <  2:2072551/98‑1 (MQ=255)                                                                            cAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATCAAATTGAACATGCCATTa                   >  1:108579/1‑100 (MQ=255)                                                                            cAAGGCTTTAAGAAGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAAAAGCCAATAACACAGCGTTAGCATGACAATAAAATTGAAAATCCCATTa                   >  2:2147735/1‑100 (MQ=255)                                                                             aaGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTAc                  >  1:278627/1‑100 (MQ=255)                                                                               ggCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGGCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGATAATAAAATTGAACATGCCATTACTc                <  2:2073877/100‑1 (MQ=255)                                                                               ggCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTc                <  2:1191261/100‑1 (MQ=255)                                                                                tcgctAAGATAAGGGAAATTGACAGACACTGCGCCGAGTATAAAGAACGGCAAATATAACATCGTTTGCATGACAATAAAATTGAACATGCCATTACTCa               <  1:2135699/96‑1 (MQ=255)                                                                                 cTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAg              >  1:253442/1‑100 (MQ=255)                                                                                     aaGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATg          >  1:1519439/1‑100 (MQ=255)                                                                                     aaGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATg          <  1:1386222/100‑1 (MQ=255)                                                                                     aaGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATg          <  1:1385206/100‑1 (MQ=255)                                                                                     aaGATCAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAAAAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATg          >  2:2282124/1‑100 (MQ=255)                                                                                       gATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATggg        <  1:2626710/100‑1 (MQ=255)                                                                                         tGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCg      <  2:1512409/100‑1 (MQ=255)                                                                                          gAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCgg     <  2:85551/100‑1 (MQ=255)                                                                                           aGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGa    <  2:2528439/100‑1 (MQ=255)                                                                                           aGGGAAAGTGAAAGCCAGGGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATATAATTGAACATGCCATTACTCAGAATGGGCGGa    <  2:2039932/100‑1 (MQ=255)                                                                                             ggAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATa  <  1:1910255/100‑1 (MQ=255)                                                                                             ggAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATa  <  1:2472337/100‑1 (MQ=255)                                                                                                  |                                                                                               ATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATA  >  NC_000913/1108150‑1108341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑