Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,264,149	G→A	C96Y (TGC→TAC)	setB →	lactose/glucose efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,264,149	0	G	A	100.0%	317.1 / NA	94	C96Y (TGC→TAC)	setB	lactose/glucose efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/51);  total (43/51)

GGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTG  >  NC_000913/2264052‑2264244                                                                                                  |                                                                                                gggATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACa                                                                                               <  2:1706417/100‑1 (MQ=255) gggATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACa                                                                                               <  1:1549949/100‑1 (MQ=255)  ggATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTacac                                                                                              >  1:759587/1‑100 (MQ=255)   gATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACAcc                                                                                             >  1:935880/1‑100 (MQ=255)    aTTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACAccc                                                                                            >  1:2266430/1‑100 (MQ=255)     ttCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCt                                                                                           >  2:1357680/1‑100 (MQ=255)     tacTGGTAAGTAAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCt                                                                                           >  2:2404765/3‑100 (MQ=255)       cTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCttt                                                                                         <  1:624017/100‑1 (MQ=255)       cTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCttt                                                                                         <  1:621383/100‑1 (MQ=255)       cTGGGAAGTCAGTTGCTGGCCGGGGGCTCTGATAATCGCGGGGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCttt                                                                                         <  2:2223677/100‑1 (MQ=255)        tGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCtttt                                                                                        >  2:338672/1‑100 (MQ=255)         ggTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCttttt                                                                                       >  1:871416/1‑100 (MQ=255)         ggTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCttttt                                                                                       >  2:1466286/1‑100 (MQ=255)          gTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTg                                                                                      <  2:93022/100‑1 (MQ=255)          gTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTg                                                                                      <  1:1386218/100‑1 (MQ=255)            aaGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGcn                                                                                    <  1:2250885/100‑2 (MQ=255)              gTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTg                                                                                  <  2:1711429/100‑1 (MQ=255)               tCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGGTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTgg                                                                                 <  1:2307621/100‑1 (MQ=255)               tCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTgg                                                                                 <  1:524906/100‑1 (MQ=255)                cAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGa                                                                                >  2:2097418/1‑100 (MQ=255)                 aGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTTATTGTCTTTTGCTGCCTGTTAGGCGTTCTGGCCTACACCCTTTTTGCCTGGaa                                                                               >  1:256088/1‑100 (MQ=255)                 aGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGTTGGCCTACACCCTTTTTGCCTGGaa                                                                               >  1:2131938/1‑100 (MQ=255)                 aGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGaa                                                                               >  2:1213802/1‑100 (MQ=255)                  gTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAAt                                                                              >  2:1074218/1‑100 (MQ=255)                      cgcGCCGGGCGCTCTGATAAGCGCGTCGAGCGCAAATCGGTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCTCa                                                                          >  2:91726/3‑100 (MQ=255)                        cGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAac                                                                        <  1:2473019/100‑1 (MQ=255)                        cGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAac                                                                        <  2:1659736/100‑1 (MQ=255)                         gCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAact                                                                       >  1:2018774/1‑100 (MQ=255)                          ccGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAacta                                                                      >  2:1370681/1‑100 (MQ=255)                            gggCGCTCCGATAAGCGCGGCGATCGCAAATCGCGGATTGTCTTTTGCTGCCTGTTAGGCGTGCTCGCCTACACCCTTTTTGCCTGGAATCGCAactact                                                                    >  2:336638/1‑100 (MQ=38)                             ggCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACtt                                                                   <  1:2077735/100‑1 (MQ=255)                              gcgcTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACttt                                                                  <  1:301055/100‑1 (MQ=255)                                  tctGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGttt                                                              <  1:1707551/100‑1 (MQ=255)                                  tctGAGAAGCGCGGCGATCTCAAATCCCTGATTCTCTTTTGCTGCCTGTTAGGCGCGCTGGCCTATACCCTTTCTGCCTGGCACCGCAACTACTTCGttt                                                              >  2:297284/1‑100 (MQ=255)                                   ctGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGtttt                                                             <  2:1458510/100‑1 (MQ=255)                                    tGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTg                                                            <  1:1956829/100‑1 (MQ=255)                                    tGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTg                                                            <  2:2101115/100‑1 (MQ=255)                                    tGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTg                                                            >  1:1646220/1‑100 (MQ=255)                                     gATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTTTTTTTTTTCTCAACTACTTTGTTTTGc                                                           >  2:1882154/1‑100 (MQ=255)                                     gATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGc                                                           <  2:597401/100‑1 (MQ=255)                                     gATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGc                                                           >  2:2494732/1‑100 (MQ=255)                                     gATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGc                                                           >  2:1708881/1‑100 (MQ=255)                                           cgcgGCGGTCGCAAATCGTTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCGACACCCTTGTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCg                                                     <  1:2142916/100‑1 (MQ=255)                                             cggcgATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGtt                                                   <  1:1134568/100‑1 (MQ=255)                                               gcgATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTgg                                                 <  2:1221564/100‑1 (MQ=255)                                               gcgATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTgg                                                 <  1:1151268/100‑1 (MQ=255)                                                     gCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGtctt                                           >  1:294805/1‑100 (MQ=255)                                                      ccaaccGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGtcttt                                          <  2:2233601/95‑1 (MQ=255)                                                      cAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGtcttt                                          <  2:790765/100‑1 (MQ=255)                                                      cAAATCGCTGATTGTCTTTTCCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAATTACTTTGTTTTGCTATTCGTTGGCGtcttt                                          >  2:424063/1‑100 (MQ=255)                                                       aaaTCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGtctttc                                         <  1:1097907/100‑1 (MQ=255)                                                         aTCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGTCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGtctttctt                                       <  1:1839575/100‑1 (MQ=255)                                                         aTCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGtctttctt                                       <  1:1820998/100‑1 (MQ=255)                                                          tCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTa                                      >  1:930365/1‑100 (MQ=255)                                                           cGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTag                                     <  1:302869/100‑1 (MQ=255)                                                            gCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTagc                                    >  1:1994321/1‑100 (MQ=255)                                                             cTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTagca                                   <  2:1124587/100‑1 (MQ=255)                                                              tGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTTTTTTTTTTTTTAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTagcag                                  >  2:137668/1‑100 (MQ=255)                                                              tGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTagcag                                  >  1:2529913/1‑100 (MQ=255)                                                               gATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTagcagc                                 <  1:1053082/100‑1 (MQ=255)                                                               gATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTagcagc                                 <  1:821364/100‑1 (MQ=255)                                                               gATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTagcagc                                 >  2:1336242/1‑100 (MQ=255)                                                                aTTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCt                                >  1:843235/1‑100 (MQ=255)                                                                  tGTCCTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTGTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCttt                              <  1:1393776/100‑1 (MQ=255)                                                                    tCTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTTCTATTCGTTGGCGTCTTTCTTAGCAGCTTTgg                            >  1:828742/1‑100 (MQ=255)                                                                     cTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGc                           <  1:2450678/100‑1 (MQ=255)                                                                     cTTTTGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGc                           <  1:1377155/100‑1 (MQ=255)                                                                      ttttGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCt                          >  1:274498/1‑100 (MQ=255)                                                                      ttttGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCt                          <  1:2127315/100‑1 (MQ=255)                                                                      ttttGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCt                          >  1:1405549/1‑100 (MQ=255)                                                                      ttttGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCt                          <  1:1580875/100‑1 (MQ=255)                                                                      ttttGCTGCCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCt                          <  1:181035/100‑1 (MQ=255)                                                                         tgctgcCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGa                       >  1:1473127/1‑100 (MQ=255)                                                                          gctgcCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGAc                      >  1:831655/1‑100 (MQ=255)                                                                          gctgcCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGAc                      <  2:739481/100‑1 (MQ=255)                                                                           ctgcCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGAcc                     >  1:1383952/1‑100 (MQ=255)                                                                           ctgcCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGAcc                     <  2:1501297/100‑1 (MQ=255)                                                                            tgcCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCg                    <  1:59693/100‑1 (MQ=255)                                                                             gcCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGc                   <  1:1107827/100‑1 (MQ=255)                                                                             gcCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGc                   >  2:784051/1‑100 (MQ=255)                                                                             gcCTGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGc                   >  2:443636/1‑100 (MQ=255)                                                                                tGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTGGTTGGCGTCTTTCTTATCAGCTTTGGCTCGACCGCTaa                >  2:1963846/1‑100 (MQ=255)                                                                                tGTTAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTaa                >  1:644460/1‑100 (MQ=255)                                                                                  ttAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAAcc              <  1:1713264/100‑1 (MQ=255)                                                                                  ttAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAAcc              <  1:1586053/100‑1 (MQ=255)                                                                                   tAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAAccc             >  2:2116288/1‑100 (MQ=255)                                                                                   tAGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAAccc             >  2:783679/1‑100 (MQ=255)                                                                                    aGGCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCg            <  1:1603712/100‑1 (MQ=255)                                                                                     ggCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGc           <  1:1860175/100‑1 (MQ=255)                                                                                     ggCGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCCCGACCGCTAACCCGc           >  1:1251032/1‑100 (MQ=255)                                                                                       cGTGCTGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCaa         >  2:123061/1‑100 (MQ=255)                                                                                            tGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGtt    <  2:2451592/100‑1 (MQ=255)                                                                                            tGGCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCCAACCCGCAAATGtt    <  1:1359746/100‑1 (MQ=255)                                                                                              gCCTACACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttg  <  1:355178/100‑1 (MQ=255)                                                                                              gCCTACACCCTTTTCGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTttg  <  2:469893/100‑1 (MQ=255)                                                                                                  |                                                                                                GGGATTCTGGTAAGTCAGTTTCTCGCCGGGCGCTCTGATAAGCGCGGCGATCGCAAATCGCTGATTGTCTTTTGCTGCCTGTTAGGCGTGCTGGCCTGCACCCTTTTTGCCTGGAATCGCAACTACTTTGTTTTGCTATTCGTTGGCGTCTTTCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTG  >  NC_000913/2264052‑2264244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑