Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,267,405	A→G	I387M (ATA→ATG)	yeiQ →	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,267,405	0	A	G	97.9%	316.7 / ‑2.6	98	I387M (ATA→ATG)	yeiQ	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (46/49);  total (47/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

CACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCAT  >  NC_000913/2267308‑2267500                                                                                                  |                                                                                                cACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCa                                                                                               >  2:424438/1‑100 (MQ=255)   cGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATc                                                                                             >  1:538774/1‑100 (MQ=255)       ttGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAggg                                                                                         <  1:292997/100‑1 (MQ=255)       ttGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAggg                                                                                         >  2:1784561/1‑100 (MQ=255)        tGCTAATCCGGCGCTGAAACATCACACGTGGTCAATTTCCATGGAGGGCAGCCAGAAGATGCCGAAACTCATGCTGACAGGTATCCGCATGCATCAgggg                                                                                        <  2:108727/100‑1 (MQ=255)        tGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAgggg                                                                                        >  1:2181250/1‑100 (MQ=255)         gCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGgc                                                                                       >  1:718620/1‑100 (MQ=255)            aaTCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGcgcg                                                                                    >  2:1623069/1‑100 (MQ=255)            aaTCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATAACCGCAACTCATGCTGGCAGGTATTCGCATGCATCAGGGGcgcg                                                                                    >  2:1190456/1‑100 (MQ=255)             aTCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGa                                                                                   >  2:1775771/1‑100 (MQ=255)             aTCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGa                                                                                   >  2:1247786/1‑100 (MQ=255)              tCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGaa                                                                                  <  1:2045601/100‑1 (MQ=255)              tCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGaa                                                                                  <  1:536913/100‑1 (MQ=255)                cGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAAc                                                                                >  1:1620081/1‑100 (MQ=255)                cGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAAc                                                                                >  2:616164/1‑100 (MQ=255)                 ggCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACg                                                                               >  2:1039149/1‑100 (MQ=255)                 ggCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACg                                                                               >  2:832169/1‑100 (MQ=255)                  gcgcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACgg                                                                              <  2:2273125/100‑1 (MQ=255)                  gcgcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACgg                                                                              >  2:1914956/1‑100 (MQ=255)                  gcgcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACgg                                                                              <  2:976389/100‑1 (MQ=255)                    gcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAGGGGCGCGAAACGGAc                                                                            <  2:549097/100‑1 (MQ=255)                     gtGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGTATGCATCAGGGGCGCGAAACGGACt                                                                           >  2:1711542/2‑100 (MQ=255)                     cTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACt                                                                           <  2:978228/100‑1 (MQ=255)                     cTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACt                                                                           <  2:1857932/100‑1 (MQ=255)                     cTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACAGACt                                                                           <  2:1859124/100‑1 (MQ=255)                       gAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTgg                                                                         <  1:1828147/100‑1 (MQ=255)                        aaaCATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGt                                                                        <  2:1722234/100‑1 (MQ=255)                        aaaCATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGt                                                                        <  2:2416751/100‑1 (MQ=255)                        aaaCATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGt                                                                        <  1:1640874/100‑1 (MQ=255)                         aaCATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTc                                                                       >  1:1547620/1‑100 (MQ=255)                         aaCATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTc                                                                       >  1:1547347/1‑100 (MQ=255)                          aCAGAAGACGGGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTAGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCg                                                                      <  2:2488644/100‑1 (MQ=255)                             tAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTg                                                                   >  2:79971/1‑100 (MQ=255)                              caGACCTGGCAAATCGCGATGGATCGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGc                                                                  <  2:451130/99‑1 (MQ=255)                                gACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTg                                                                >  1:2483198/1‑100 (MQ=255)                                 aCCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTgg                                                               <  1:1108805/100‑1 (MQ=255)                                  ccTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGc                                                              <  2:704817/100‑1 (MQ=255)                                  ccTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGc                                                              <  2:1872708/100‑1 (MQ=255)                                     ggCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCAtt                                                           <  1:1906074/100‑1 (MQ=255)                                      gCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTa                                                          <  2:987676/100‑1 (MQ=255)                                         aaTCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGc                                                       <  1:303598/100‑1 (MQ=255)                                             gcgATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTg                                                   <  1:240233/100‑1 (MQ=255)                                             gcgATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTg                                                   <  1:1654473/100‑1 (MQ=255)                                             gcgATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTg                                                   <  1:1498003/100‑1 (MQ=255)                                             gcgATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTg                                                   <  1:1952822/100‑1 (MQ=255)                                              cgATGGATGGCAGCCAGAAGTTACCGTGGCGCATGATGGCAGGCATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGc                                                  <  2:442360/100‑1 (MQ=255)                                              cgATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGc                                                  >  1:2208605/1‑100 (MQ=255)                                               gatggatgGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCa                                                 <  1:1568024/100‑1 (MQ=255)                                                atggatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAg                                                >  1:1204092/1‑100 (MQ=255)                                                atggatggCAGCCAGAAACTACCGCAACGCATGCTGGCAGGTATTCGGATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCan                                                <  2:2547882/100‑2 (MQ=255)                                                 tggatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAgg                                               >  1:2510628/1‑100 (MQ=255)                                                  ggatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGTCTGGTCGTTGCTGGCATTAGGCGTTGCAGGc                                              >  2:707203/1‑100 (MQ=255)                                                  ggatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGc                                              >  2:2452100/1‑100 (MQ=255)                                                  ggatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGc                                              >  2:2326065/1‑100 (MQ=255)                                                   gatgtCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCTTTGCAGGCt                                             <  2:1166547/100‑1 (MQ=255)                                                   gatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCt                                             >  1:2087017/1‑100 (MQ=255)                                                   gatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCt                                             >  1:1738073/1‑100 (MQ=255)                                                    atggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTg                                            <  2:356803/100‑1 (MQ=255)                                                     tggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTgg                                           <  2:619370/100‑1 (MQ=255)                                                      ggCAGCCGGAAATCACCGCACCGCGTTCTAGCAGTTTTTCGCATTCCTGAGTGGCGCGATACGGACTCGTCGGTGCTGGCATTAGGCGTGGCAAGCTGGa                                          <  2:499836/100‑1 (MQ=255)                                                       gCAGACAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCCCGAAACCAACTGGTCGTTGCTGGCCTTAGGCGTTGCCGGCTggct                                         >  2:1557885/1‑98 (MQ=255)                                                        cAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATg                                        >  1:152008/1‑100 (MQ=255)                                                        cAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATg                                        >  2:2232681/1‑100 (MQ=255)                                                           ccAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGt                                     >  1:1286175/1‑100 (MQ=255)                                                            cAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGtt                                    >  2:287742/1‑100 (MQ=255)                                                            cAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGtt                                    >  1:3947/1‑100 (MQ=255)                                                              gAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTAc                                  <  2:2529323/100‑1 (MQ=255)                                                               aaaTTACCGCAACGCATGTTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACg                                 <  1:2272139/100‑1 (MQ=255)                                                               aaaTTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACg                                 <  1:1834049/100‑1 (MQ=255)                                                                 aTTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGTTGGATGCGTTACGTc                               >  2:2154227/1‑100 (MQ=255)                                                                     ccGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcg                           >  1:2003773/1‑100 (MQ=255)                                                                       gCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcggc                         >  1:2190681/1‑100 (MQ=255)                                                                       gCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcggc                         >  2:1435802/1‑100 (MQ=255)                                                                       gCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcggc                         <  1:1236742/100‑1 (MQ=255)                                                                        cAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcggcg                        <  1:2533888/100‑1 (MQ=255)                                                                         aaCGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGt                       >  1:2330690/1‑100 (MQ=255)                                                                          aCGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGtt                      <  1:1339700/100‑1 (MQ=255)                                                                           cGCATGCTGGCAGGTATTCGCAGGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGTCTGGATGCGTTACGTCAGCGGCGTtg                     <  2:527816/100‑1 (MQ=255)                                                                            gCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTtga                    <  1:548260/100‑1 (MQ=255)                                                                             cATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTtgat                   >  1:1047312/1‑100 (MQ=255)                                                                             cATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTtgat                   <  2:1597866/100‑1 (MQ=255)                                                                              aTGCTGGCAGGTATTCGCATGCATCAGAGCCGCGAAACGGACTGGTCGTTGCTGGCATCAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTtgatg                  <  2:2468417/100‑1 (MQ=255)                                                                               tGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAATGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTtgatga                 >  2:2520933/1‑100 (MQ=255)                                                                                  tGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGc              <  1:2276106/100‑1 (MQ=255)                                                                                   ggCCGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGcc             <  1:1446008/100‑1 (MQ=255)                                                                                   ggCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGcc             <  1:299508/100‑1 (MQ=255)                                                                                   ggCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGcc             >  1:638834/1‑100 (MQ=255)                                                                                   ggCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGcc             <  2:66022/100‑1 (MQ=255)                                                                                     cAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGGTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCgg           <  1:721022/100‑1 (MQ=255)                                                                                      aGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGa          <  1:743374/100‑1 (MQ=255)                                                                                      aGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGa          <  2:2267262/100‑1 (MQ=255)                                                                                       ggTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGaa         >  2:708190/1‑100 (MQ=255)                                                                                       ggTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGaa         >  1:635949/1‑100 (MQ=255)                                                                                          aTTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATg      >  1:1799204/1‑100 (MQ=255)                                                                                            tCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGcc    <  1:474120/100‑1 (MQ=255)                                                                                             cGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCa   >  1:1938656/1‑100 (MQ=255)                                                                                             cGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCa   >  2:1585129/1‑100 (MQ=255)                                                                                             cGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTAGATGATGCCGGAAATGCCa   >  2:2322096/1‑100 (MQ=255)                                                                                              gcatgcatCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCAt  <  2:2232326/100‑1 (MQ=255)                                                                                                  |                                                                                                CACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCAT  >  NC_000913/2267308‑2267500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑