Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	145,691	C→T	A204V (GCC→GTC)	yadE →	putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	145,691	0	C	T	98.9%	307.4 / NA	93	A204V (GCC→GTC)	yadE	putative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (51/41);  minor base G (1/0);  total (52/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGAT  >  NC_000913/145595‑145787                                                                                                 |                                                                                                 tGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTg                                                                                               <  2:631998/100‑1 (MQ=255)  gCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTgg                                                                                              >  2:1088994/1‑100 (MQ=255)  gCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTgg                                                                                              >  1:1600728/1‑100 (MQ=255)       ccTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCg                                                                                         <  1:1407795/100‑1 (MQ=255)       ccTATCACCATATTCTGCCCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCg                                                                                         <  2:1681181/100‑1 (MQ=255)        cTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGt                                                                                        <  2:967329/100‑1 (MQ=255)         tATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTg                                                                                       <  1:315653/100‑1 (MQ=255)         tATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTg                                                                                       <  2:1506097/100‑1 (MQ=255)         tATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGACGGTCTGGCTGCGTg                                                                                       >  2:695524/1‑100 (MQ=255)           tCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGAc                                                                                     <  1:1204370/100‑1 (MQ=255)            cACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACa                                                                                    >  1:2220504/1‑100 (MQ=255)            cACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACa                                                                                    <  1:113667/100‑1 (MQ=255)             aCCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAg                                                                                   >  1:1953730/1‑100 (MQ=255)              ccATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGCCAgg                                                                                  >  2:1612107/1‑100 (MQ=255)              ccATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAgg                                                                                  >  1:636755/1‑100 (MQ=255)               cagatTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGGCTGGCTGCGTGACAggg                                                                                 >  2:933677/4‑100 (MQ=255)                  atTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGAt                                                                              >  2:2077176/1‑100 (MQ=255)                      tGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcgc                                                                          <  2:327697/100‑1 (MQ=255)                      tGCGCGACGAAGAAAACACCCGTTTCCGCCATACTTCGACGACCACATCGGTACGCGCTCTCACTACCCAGATGGTCTGGCTGCGTGCCAGGGGATAcgc                                                                          >  2:866418/1‑100 (MQ=255)                       gcgcGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcgcg                                                                         <  1:1683898/100‑1 (MQ=255)                       gcgcGACGAAGAAAACACCCGTTTTCGCCATACTTCCACCACCACATCCGTACGCGCTTTCAATAACCAGATGTTCTGGCTGCGTGACAGGTGATAcgcg                                                                         <  2:1167556/100‑1 (MQ=255)                        cgcgACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGa                                                                        >  1:1305112/1‑100 (MQ=255)                        cgcgACGAAGAAAACACACGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGa                                                                        >  2:2065848/1‑100 (MQ=255)                           gacgaAGACAGCACCCGGTTTCGCCATACATCGACGACCACATGGGTACGCGCTTTCAATAACCAGATGGTATGGCTGCGTGACAGGGGATAAGCGacac                                                                     <  2:1202279/100‑1 (MQ=255)                           gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGacac                                                                     <  2:1196814/100‑1 (MQ=255)                           gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGacac                                                                     >  2:801935/1‑100 (MQ=255)                           gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGacac                                                                     >  2:2134212/1‑100 (MQ=255)                            acgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACt                                                                    >  2:2063706/1‑100 (MQ=255)                               aagaaAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAg                                                                 <  1:1611849/100‑1 (MQ=255)                               aagaaAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAg                                                                 <  2:2140139/100‑1 (MQ=255)                                 gaaAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCa                                                               <  1:1804498/100‑1 (MQ=255)                                    aaCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATgg                                                            <  1:2089624/100‑1 (MQ=255)                                     acacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGt                                                           <  2:1529672/100‑1 (MQ=255)                                      cacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTg                                                          <  1:2194238/100‑1 (MQ=255)                                      cacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTg                                                          >  1:1450544/1‑100 (MQ=255)                                          cGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGc                                                      >  2:276689/1‑100 (MQ=255)                                            ttttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTg                                                    <  1:1005858/100‑1 (MQ=255)                                              ttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGa                                                  >  1:1935771/1‑100 (MQ=255)                                                cGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAg                                                <  1:880559/100‑1 (MQ=255)                                                cGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAg                                                >  2:599758/1‑100 (MQ=255)                                                cGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAg                                                <  2:2375693/100‑1 (MQ=255)                                                cGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAg                                                <  2:1817508/100‑1 (MQ=255)                                                 gCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGCTACGCGACACTGAGCATGGTGCAGCTGGAAgg                                               >  2:67726/1‑100 (MQ=255)                                                 gCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAgg                                               <  2:2390467/100‑1 (MQ=255)                                                 gCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGATGGAAgg                                               >  2:1363341/1‑100 (MQ=255)                                                  ccATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGc                                              >  2:1819221/1‑100 (MQ=255)                                                   cATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGCTGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCt                                             >  2:1025735/1‑100 (MQ=255)                                                   cATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCt                                             <  1:738045/100‑1 (MQ=255)                                                     tACTTCTACGAGCACATCGGTAAGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACAGATCATAGTGGAGCTAGAAgacaac                                           >  2:2135657/1‑95 (MQ=255)                                                     tACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTAc                                           <  2:2348348/100‑1 (MQ=255)                                                     tACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTAc                                           >  2:933618/1‑100 (MQ=255)                                                       cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGt                                         >  1:1717039/1‑100 (MQ=255)                                                       cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGt                                         <  2:1601935/100‑1 (MQ=255)                                                       cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGt                                         >  1:331247/1‑100 (MQ=255)                                                       cTTCGACGAACACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGt                                         >  1:2580101/1‑100 (MQ=255)                                                        ttCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTg                                        <  2:26013/100‑1 (MQ=255)                                                           gacgacCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaag                                     <  1:750844/100‑1 (MQ=255)                                                            acgacCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaaga                                    <  2:2102485/100‑1 (MQ=255)                                                             cgacCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaagaa                                   >  2:1253956/1‑100 (MQ=255)                                                              gacCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAAt                                  >  1:1448658/1‑100 (MQ=255)                                                                ccACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATaa                                >  1:1679605/1‑100 (MQ=255)                                                                   caccGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGAt                             >  2:1428328/4‑100 (MQ=255)                                                                   caTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGAt                             >  2:2490539/1‑100 (MQ=255)                                                                    aTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTCCGTGAAGAATAAGATc                            >  1:2172547/1‑100 (MQ=255)                                                                    aTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATc                            <  1:2333225/100‑1 (MQ=255)                                                                    aTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATc                            <  2:623894/100‑1 (MQ=255)                                                                       ggTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAt                         >  2:733979/1‑100 (MQ=255)                                                                        gTACGCGCGTTCAATAACCAGATGGTCTGGCTGCGCGACAGGAGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGGGAAGAATAAGATCaacc                        <  2:1440281/100‑3 (MQ=255)                                                                           cgcgCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTcc                     >  1:1465998/1‑100 (MQ=255)                                                                            gcgcTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATGTccc                    >  2:1645476/1‑100 (MQ=255)                                                                            gcgcTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTccc                    <  2:2203268/100‑1 (MQ=255)                                                                            gcgcTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTccc                    >  2:1645559/1‑100 (MQ=255)                                                                              gcTTTCAATAGCCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTg                  <  2:1610265/100‑1 (MQ=255)                                                                                tttCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGACGGCTACGTGAAGAATAAGATCAATCTCCCTgcg                >  2:1163199/1‑100 (MQ=255)                                                                                tttCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTgcg                <  1:2454254/100‑1 (MQ=255)                                                                                tttCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTgcg                >  2:1617171/1‑100 (MQ=255)                                                                                 ttCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTgcgc               >  2:835434/1‑100 (MQ=255)                                                                                  tCAATAACCAGATGGTCTGGCTTCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTTcgcg              >  2:435020/1‑100 (MQ=255)                                                                                  tCAATAACCAGAGGGTCTGGCTGCGTGACAGGGGATACGCTACACTGAGCATGGTGCACTTGGAAGGCTACGTGTAGAATAAGATCAATCACCCTGcgcg              <  2:702495/100‑1 (MQ=255)                                                                                   cAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGa             >  2:719081/1‑100 (MQ=255)                                                                                     aTAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGc           >  2:1738378/1‑100 (MQ=255)                                                                                      tAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCg          >  1:1669079/1‑100 (MQ=255)                                                                                        aCCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggt        >  1:1079584/1‑100 (MQ=255)                                                                                        aCCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggt        >  1:1731110/1‑100 (MQ=255)                                                                                         ccAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggtg       >  2:788611/1‑100 (MQ=255)                                                                                          cAGATGGTCTGGCTGCGTGACGGGGGATACGCGACACTGAGCATGGTGCAGCTGCGAGTCTACGTGAAGAATAAGATCAATCTCACTGCGCGAGtggtgg      >  2:2472704/1‑100 (MQ=255)                                                                                          cAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggtgg      <  1:1395365/100‑1 (MQ=255)                                                                                          cAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggtgg      <  2:1929044/100‑1 (MQ=255)                                                                                          cAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggtgg      >  1:1672564/1‑100 (MQ=255)                                                                                          cAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggtgg      >  1:441375/1‑100 (MQ=255)                                                                                             aTGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGGTCAATCTCCCTGCGCGAGCGGTGGTGa   <  2:1323526/100‑1 (MQ=255)                                                                                              tGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGAt  <  1:889200/100‑1 (MQ=255)                                                                                              tGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGAt  >  2:462249/1‑100 (MQ=255)                                                                                                 |                                                                                                 TGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGAT  >  NC_000913/145595‑145787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑