Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,566,416 T→C T157A (ACC→GCC)  eutA ← reactivating factor for ethanolamine ammonia lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,566,4160TC97.9% 321.5 / ‑4.6 95T157A (ACC→GCC) eutAreactivating factor for ethanolamine ammonia lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (35/58);  minor base G (1/1);  total (36/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGT  >  NC_000913/2566319‑2566511
                                                                                                 |                                                                                               
aCGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGgcgc                                                                                               >  1:1469918/1‑100 (MQ=255)
 cgcgCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGcc                                                                                              <  1:270670/100‑1 (MQ=255)
 cgcgCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGcc                                                                                              <  1:681075/100‑1 (MQ=255)
 cgcgCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGcc                                                                                              >  1:1551817/1‑100 (MQ=255)
       cGTGGCTGTCGGTTTCCAGCAGGCGTCCCCCGACGTTTCGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCAccgcc                                                                                        <  1:255336/100‑1 (MQ=255)
       cGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCAccgcc                                                                                        <  1:1601851/100‑1 (MQ=255)
       cGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCAccgcc                                                                                        <  2:725384/100‑1 (MQ=255)
       cGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCAccgcc                                                                                        <  2:725226/100‑1 (MQ=255)
       agTGGCTGTCGGTTGCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCTGCTGATTTTTCCAGCATCGAACAGGGAGTAGTTCGCGGCGCAAccgcc                                                                                        <  2:479908/99‑1 (MQ=255)
        gTGGCTGTCGGTTACCAGCAGGCGCCCACCTACGTTGATGCACGCAGTGCCGCTCATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCAccgccg                                                                                       <  2:1691169/100‑1 (MQ=255)
           gCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGGGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACTGGGCGTAGTTCGCGGCGCCACCGCCGATg                                                                                    <  2:2567743/100‑1 (MQ=255)
           gCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATg                                                                                    <  2:937/100‑1 (MQ=255)
           gCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATg                                                                                    <  1:737691/100‑1 (MQ=255)
           gCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATg                                                                                    <  2:949/100‑1 (MQ=255)
           gCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATg                                                                                    <  2:1754802/100‑1 (MQ=255)
           gCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATg                                                                                    <  1:2536047/100‑1 (MQ=255)
            cTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCGTCGAACCGGGCGTCGTTCGCGGGGTCACCGCCGACGt                                                                                   >  2:1543434/1‑100 (MQ=255)
            cTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGt                                                                                   <  1:2488504/100‑1 (MQ=255)
            cTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGt                                                                                   >  1:1892841/1‑100 (MQ=255)
             tGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTc                                                                                  >  1:2561996/1‑100 (MQ=255)
              gTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCg                                                                                 <  1:423449/100‑1 (MQ=255)
              gTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCg                                                                                 <  1:973174/100‑1 (MQ=255)
               tCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGa                                                                                >  2:127703/1‑100 (MQ=255)
                cGGTTTCCAGCAGGCGACCTCCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTTCGGCATCGAACAGAGCGTAGTTCGCGGCGCCACCGCCGATGTCGat                                                                               <  1:2348232/100‑1 (MQ=255)
                cGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGat                                                                               >  1:1626159/1‑100 (MQ=255)
                 ggTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGata                                                                              >  1:1131722/1‑100 (MQ=255)
                  gTTTCCAGCAGGCGATCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGatat                                                                             <  1:1837405/100‑1 (MQ=255)
                  gTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGatat                                                                             <  2:197817/100‑1 (MQ=255)
                    ttCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTc                                                                           <  2:967711/100‑1 (MQ=255)
                    ttCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTc                                                                           <  1:922282/100‑1 (MQ=255)
                      ccAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAg                                                                         <  2:895645/100‑1 (MQ=255)
                       cagcagGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGt                                                                        >  2:1642372/1‑100 (MQ=255)
                       cagcagGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGt                                                                        >  1:1924770/1‑100 (MQ=255)
                        agcagGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTa                                                                       >  2:420673/1‑100 (MQ=255)
                         gcagGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTAc                                                                      <  2:751178/100‑1 (MQ=255)
                           agGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTAccc                                                                    <  2:882047/100‑1 (MQ=255)
                           agGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTAccc                                                                    <  1:761458/100‑1 (MQ=255)
                           acgctaccaccGACGGCGAGGCTGCCTGTGCCACTGATTTTTCCTGCTTCGAACAGGGCGTAGTTCGCGGGGCCACCGCCGATGTCGATATTCATTTccc                                                                    <  2:445356/95‑1 (MQ=255)
                            ggCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCg                                                                   >  2:1222677/1‑100 (MQ=255)
                              cGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGac                                                                 <  2:2493731/100‑1 (MQ=255)
                                 ccaccGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGAcaca                                                              >  1:1531412/1‑100 (MQ=255)
                                  caccGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAg                                                             >  1:1081885/1‑100 (MQ=255)
                                  caccGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAg                                                             >  1:1071319/1‑100 (MQ=255)
                                   accGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGc                                                            <  1:487640/100‑1 (MQ=255)
                                   accGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGc                                                            >  1:19412/1‑100 (MQ=255)
                                   accGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGc                                                            <  1:487675/100‑1 (MQ=255)
                                    ccGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGcc                                                           >  2:2288888/1‑100 (MQ=255)
                                     cGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCg                                                          <  2:357954/100‑1 (MQ=255)
                                         gTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCgttgt                                                      >  1:1754965/1‑100 (MQ=255)
                                          ttGAGGCAGGCAGTGCCGCTGATTTTTTTTTTTTTTTTCAGGGCGTAGTTCGCGGCGCCACCGCCGGTGTCGATATTCAGTACCCGACACAGCCgttgtt                                                     >  2:1604564/1‑100 (MQ=255)
                                          ttGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCgttgtt                                                     <  1:2162247/100‑1 (MQ=255)
                                           tGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTc                                                    <  2:128533/100‑1 (MQ=255)
                                            gAGGCAGGCAGTGCCGCTGATTTTTCCGGCAGCGAACAGGGCTTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCa                                                   <  2:928615/100‑1 (MQ=255)
                                             aggcaggcAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAg                                                  <  1:1514929/100‑1 (MQ=255)
                                              ggcaggcaGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGa                                                 <  2:857841/100‑1 (MQ=255)
                                              ggcaggcaGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGa                                                 >  2:2107453/1‑100 (MQ=255)
                                              ggcaggcaGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGa                                                 >  1:2051009/1‑100 (MQ=255)
                                                   gcagTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAggg                                            <  2:1999048/100‑1 (MQ=255)
                                                      gTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGttt                                         >  2:45892/1‑100 (MQ=255)
                                                       tGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTtg                                        <  1:2601809/100‑1 (MQ=255)
                                                       tGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTtg                                        <  2:478040/100‑1 (MQ=255)
                                                           gCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGcc                                    >  1:1498152/1‑100 (MQ=255)
                                                             tGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAACGGGTTTGTGcccc                                  >  2:1780487/1‑100 (MQ=255)
                                                             tGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGcccc                                  >  2:814721/1‑100 (MQ=255)
                                                              gATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCg                                 <  1:704960/100‑1 (MQ=255)
                                                              gATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCg                                 <  2:1530805/100‑1 (MQ=255)
                                                              gATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCg                                 >  2:548959/1‑100 (MQ=255)
                                                              gATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCg                                 <  1:2228461/100‑1 (MQ=255)
                                                                  tttCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTc                             >  2:259220/1‑100 (MQ=255)
                                                                     ccGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGt                          >  2:1200362/1‑100 (MQ=255)
                                                                     ccGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGt                          <  2:1685952/100‑1 (MQ=255)
                                                                      cGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTg                         >  2:838820/1‑100 (MQ=255)
                                                                      cGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTg                         <  2:1652357/100‑1 (MQ=255)
                                                                      cGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTg                         >  2:1531275/1‑100 (MQ=255)
                                                                       ggCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGa                        >  1:2551259/1‑100 (MQ=255)
                                                                         cATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGAcc                      <  2:570482/100‑1 (MQ=255)
                                                                          aTCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCg                     <  2:1976558/100‑1 (MQ=255)
                                                                             gAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCg                  >  2:908079/1‑100 (MQ=255)
                                                                             gAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCg                  >  1:1194136/1‑100 (MQ=255)
                                                                             gAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCg                  >  2:2390338/1‑100 (MQ=255)
                                                                             gAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCg                  >  1:815171/1‑100 (MQ=255)
                                                                             gAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCg                  <  2:1899453/100‑1 (MQ=255)
                                                                              aaCAGGGCGTAGTTCGCGGCGCCACCGCCGGTGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGa                 <  2:675311/100‑1 (MQ=255)
                                                                               aCAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGAt                <  2:1337920/100‑1 (MQ=255)
                                                                                   ggggTAGTTCGCGGCGCCACCGCCTATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACg            <  2:2173646/97‑1 (MQ=255)
                                                                                   ggggTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACg            <  1:390750/97‑1 (MQ=255)
                                                                                     cGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGCTGTTAAGAAAGGGTTTGTGCCGCGGCTCCGTGACCGGCGATCACGGa          >  2:1376436/1‑100 (MQ=255)
                                                                                      gTAGTTGGCGGCGGCAAGGCCGGTGTCGGTAGTCGGTACCCGCCACAGCAGTTGTTCCGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGAt         <  2:921858/100‑1 (MQ=255)
                                                                                       tAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGATCGCCGGTCACGGagc        <  2:2492107/100‑3 (MQ=255)
                                                                                       tAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGATCGCCGGTCACGGagc        <  1:893611/100‑3 (MQ=255)
                                                                                        aGTTTGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGTCGTTGTTCAGAAAGGGTTTGTGCCCCGGTTCCGTTACCGGCGATCACGGATTc       <  2:1151781/100‑1 (MQ=255)
                                                                                         gTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCg      <  2:1731304/100‑1 (MQ=255)
                                                                                         gTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCg      <  1:1908934/100‑1 (MQ=255)
                                                                                          ttCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGa     >  1:2072093/1‑100 (MQ=255)
                                                                                            ggcggcgCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAgg   >  2:1195120/2‑100 (MQ=255)
                                                                                            cgcgGCGCGACCGCTCATGTGGATATTCGGTTCCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCCCCGTGAGCGGCGATCACGGATTCGAgg   <  2:1646494/100‑1 (MQ=255)
                                                                                            cgcgGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAgg   <  2:1842836/100‑1 (MQ=255)
                                                                                             gcggcgCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGt  >  1:2603433/1‑100 (MQ=255)
                                                                                                 |                                                                                               
ACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGT  >  NC_000913/2566319‑2566511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: