Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,035,546 T→C T246A (ACG→GCG)  prfB ← peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,035,5460TC98.0% 335.8 / ‑5.4 103T246A (ACG→GCG) prfBpeptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (45/55);  total (47/56)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTG  >  NC_000913/3035449‑3035642
                                                                                                 |                                                                                                
cGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACgcgc                                                                                                <  1:1700734/100‑1 (MQ=255)
 ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAcgcgcg                                                                                               >  2:1849543/1‑100 (MQ=255)
 ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAcgcgcg                                                                                               >  2:1576726/1‑100 (MQ=255)
 ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAcgcgcg                                                                                               <  1:2214892/100‑1 (MQ=255)
  gTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGa                                                                                              >  2:287663/1‑100 (MQ=255)
    cGTTCTGGCACTGGGTCACGATCCCGGTGGGGATGTGGGTAATACGCACCGCAGATTCGGCACGGTTAACGTGCTGACCGCCGGCGCCGGACGCGCGATa                                                                                            >  2:1472489/1‑100 (MQ=255)
    cGTTCTGGCACTGGGTCACGATCCCCGTCGGGATCTGGGTAATACGCCCCGCAGATTCGGTACGGGTAACGTGCTGACCGCCCGCGCCGGACGCGCGATa                                                                                            >  2:1621485/1‑100 (MQ=255)
       tCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAAc                                                                                         <  1:52232/100‑1 (MQ=255)
        cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACg                                                                                        >  1:456763/1‑100 (MQ=255)
        cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACg                                                                                        <  2:356741/100‑1 (MQ=255)
         tGGCAGTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGt                                                                                       >  2:2279115/1‑100 (MQ=255)
          ggCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTc                                                                                      >  1:1219034/1‑100 (MQ=255)
            cACTGGGTCGCGGTTCTGGGCGGGCTGTGGGTAATAGGCACGGCAAATTCGGGACGGTTAACGGCCTGACCGCCCGCGCCGGACGCGCAACAAACGTCaa                                                                                    >  2:640086/1‑100 (MQ=255)
            cACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCaa                                                                                    <  2:1651490/100‑1 (MQ=255)
            cACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCaa                                                                                    >  2:1351157/1‑100 (MQ=255)
            cACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCaa                                                                                    <  2:1041255/100‑1 (MQ=255)
             aCTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAAt                                                                                   >  2:1630551/1‑100 (MQ=255)
             aCTGGGTCACGATCCCGGGCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGCTACACGTCAAt                                                                                   >  2:1630275/1‑100 (MQ=255)
              cTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATg                                                                                  <  2:669200/100‑1 (MQ=255)
              cTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATg                                                                                  <  2:2302145/100‑1 (MQ=255)
              cTGGGTCACGAGCCCGGTCGGGATGTGGGTAACACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATg                                                                                  <  2:2302278/100‑1 (MQ=255)
                  gTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCa                                                                              <  1:2253709/100‑1 (MQ=255)
                  gTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCa                                                                              >  2:1347707/1‑100 (MQ=255)
                   tCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGCTAAACGTCAATGCGCAg                                                                             >  2:443911/1‑100 (MQ=255)
                   tCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAg                                                                             <  2:472760/100‑1 (MQ=255)
                      cGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATc                                                                          >  2:506782/1‑100 (MQ=255)
                       gATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATcc                                                                         <  2:1324849/100‑1 (MQ=255)
                        aTCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATccg                                                                        <  2:1941190/100‑1 (MQ=255)
                            cGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCgg                                                                    <  1:2533279/100‑1 (MQ=255)
                              gTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGt                                                                  <  1:1598689/100‑1 (MQ=255)
                              gTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGt                                                                  <  1:640572/100‑1 (MQ=255)
                               tCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGtt                                                                 <  1:1850192/100‑1 (MQ=255)
                                cGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTg                                                                >  2:1224897/1‑100 (MQ=255)
                                cGGGAGGGGGGTAATACGGACCGCAGAGTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTg                                                                <  2:1749073/100‑1 (MQ=255)
                                 gggATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGa                                                               <  2:2140316/100‑1 (MQ=255)
                                  ggATGTGGGTAATACGCACCGCAGATTCGGTACGGGGAACGTGCTGACCGCCCCCGCAGGACGCGCGATAAACGTCAAGGCGCAGATCCGCCgggcttat                                                              >  2:1804212/1‑95 (MQ=255)
                                   gATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAtt                                                             >  1:238558/1‑100 (MQ=255)
                                    aTGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAttt                                                            >  2:1747172/1‑100 (MQ=255)
                                      gtgGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCg                                                          <  1:1792455/100‑1 (MQ=255)
                                      gtgGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCg                                                          <  1:2593544/100‑1 (MQ=255)
                                      gtgGGTAATACGCACCGCAGAATCGGTACGGTTAACGTGATGGCCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCg                                                          <  2:1901586/100‑1 (MQ=255)
                                       tgGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                         <  1:1734612/100‑1 (MQ=255)
                                       tgGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                         >  2:1411929/1‑100 (MQ=255)
                                        gggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                        <  1:704957/100‑1 (MQ=255)
                                        gggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                        >  1:1376119/1‑100 (MQ=255)
                                        gggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                        <  2:1807290/100‑1 (MQ=255)
                                        gggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                        >  2:553360/1‑100 (MQ=255)
                                        gggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                        >  2:1328358/1‑100 (MQ=255)
                                          gTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatat                                                      <  1:1842077/100‑1 (MQ=255)
                                           tAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatc                                                     >  1:2545703/1‑100 (MQ=255)
                                            aaTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATATACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                                    <  1:2306061/100‑1 (MQ=255)
                                            aaTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATACACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                                    <  1:2306142/100‑1 (MQ=255)
                                            aaTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                                    <  1:955790/100‑1 (MQ=255)
                                            aaTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                                    <  1:894444/100‑1 (MQ=255)
                                            aaTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                                    <  2:800281/100‑1 (MQ=255)
                                              tACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaat                                                  >  2:885271/1‑100 (MQ=255)
                                               aCGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGCGTTGATTTCGatatcaata                                                 <  2:2376114/100‑1 (MQ=255)
                                                ngCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGGTGATTTCGatatcaatgt                                                >  1:2113480/2‑98 (MQ=255)
                                                cGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatat                                                >  1:1666350/1‑100 (MQ=255)
                                                cGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatat                                                >  2:16891/1‑100 (MQ=255)
                                                cGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatat                                                >  1:762914/1‑100 (MQ=255)
                                                cGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatat                                                >  1:2142561/1‑100 (MQ=255)
                                                 gCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatatc                                               >  1:2411162/1‑100 (MQ=255)
                                                    ccGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatc                                            >  1:479184/1‑100 (MQ=255)
                                                     cGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatca                                           <  2:689259/100‑1 (MQ=255)
                                                      gCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatcat                                          >  1:953703/1‑100 (MQ=255)
                                                      gCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatcat                                          >  1:658436/1‑100 (MQ=255)
                                                           ttCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTAGATATCAATATCATCATCAACt                                     >  2:1148278/1‑100 (MQ=255)
                                                             cGGTAGGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCACTGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTc                                   <  1:2507284/100‑1 (MQ=255)
                                                             cGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTc                                   <  1:361265/100‑1 (MQ=255)
                                                               gTACGGTTAACGTGCTGACCGCCCGCGCGGGACGCGCGATACACGTCAATGCGCAGTTCCGCCGGGTTGATTTCGTTATCAATATCGTCATCAACTTCCg                                 <  2:2298404/100‑1 (MQ=255)
                                                                tACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCgg                                <  2:331991/100‑1 (MQ=255)
                                                                tACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCgg                                >  1:1115350/1‑100 (MQ=255)
                                                                  cGGTTAACGTGCTGACCGCCCGCGCGGGACGCGCGATCAACGTCAATGCGCAGATCCGCCGGGTTCATTTCGATATCAATATCATCATCAACTTCCGGAt                              <  1:2455461/100‑1 (MQ=255)
                                                                  cGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGAt                              <  2:690926/100‑1 (MQ=255)
                                                                  cGGTTAACGTGCTGACCGCCCGCGCCGAACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGAt                              <  2:95346/100‑1 (MQ=255)
                                                                   ggTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATa                             >  2:2330248/1‑100 (MQ=255)
                                                                     ttAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaa                           <  1:1488017/100‑1 (MQ=255)
                                                                     ttAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaa                           <  1:743992/100‑1 (MQ=255)
                                                                     ttAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGAGCCGCCAGGCTGATTTCGATATCAATATCATCATCCACTTCCGGCTaaa                           >  1:1054335/1‑100 (MQ=255)
                                                                      tAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaac                          <  1:1506247/100‑1 (MQ=255)
                                                                      tAACGTGCCCACCGCCCGCGCCGGACGCGCGTTAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaac                          <  2:498668/100‑1 (MQ=255)
                                                                          gTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaacaaac                      >  2:2173693/1‑100 (MQ=255)
                                                                          gTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaacaaac                      >  1:1818244/1‑100 (MQ=255)
                                                                           tGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAcg                     >  1:892560/1‑100 (MQ=255)
                                                                            gCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAcgc                    <  2:1543500/100‑1 (MQ=255)
                                                                            gCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAcgc                    <  1:1219523/100‑1 (MQ=255)
                                                                              tGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAGGCgg                  <  2:2207396/100‑1 (MQ=255)
                                                                               gACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGa                 <  1:2283024/100‑1 (MQ=255)
                                                                               gACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGa                 >  1:1918083/1‑100 (MQ=255)
                                                                                 ccgcccggGCCGGACGGGCGATAAGCGTAAATGCGGAGATGCTACGGGTTGATTTCGATATCAATATCTTCGTCGACTTCTGGATAAGCAAACGCGGAGc               <  2:740767/100‑1 (MQ=255)
                                                                                   gcccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTg             <  1:317911/100‑1 (MQ=255)
                                                                                   gcccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTg             >  1:1290515/1‑100 (MQ=255)
                                                                                     ccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGaa           >  1:518939/1‑100 (MQ=255)
                                                                                      cgcgCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAac          <  1:924861/100‑1 (MQ=255)
                                                                                       gcgcCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacg         <  1:996974/100‑1 (MQ=255)
                                                                                        cgcCGGACGCGCGATAAACGTCAATGCGCAGACCCGCCGGGTTGATTTCGATATCAATATCATCATCATCTTCCGGATAAACAAACGCGGAGCTGAacga        <  1:2541885/100‑1 (MQ=255)
                                                                                          ccGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacgacg      <  1:2226610/100‑1 (MQ=255)
                                                                                          ccGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacgacg      <  1:1555096/100‑1 (MQ=255)
                                                                                              aCGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTGGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGtgtg  >  2:495491/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTG  >  NC_000913/3035449‑3035642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑