Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,173,566 G→C R611G (CGC→GGC)  parE ← DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,173,5660GC99.2% 427.8 / ‑4.0 124R611G (CGC→GGC) parEDNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (64/59);  total (64/60)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 124 total aligned reads displayed.

GCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAA  >  NC_000913/3173470‑3173661
                                                                                                |                                                                                               
gCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCttt                                                                                              >  1:608501/1‑100 (MQ=255)
gCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCttt                                                                                              >  1:1368768/1‑100 (MQ=255)
 cccGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTttc                                                                                             >  1:925944/1‑100 (MQ=255)
 cccGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTttc                                                                                             >  2:1502003/1‑100 (MQ=255)
 cccGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTttc                                                                                             >  2:1502005/1‑100 (MQ=255)
 cccGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTttc                                                                                             >  1:1610568/1‑100 (MQ=255)
   cGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTttctt                                                                                           <  2:1305896/100‑1 (MQ=255)
    ggCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTttcttc                                                                                          <  1:267514/100‑1 (MQ=255)
       cATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGcc                                                                                       <  2:1209436/100‑1 (MQ=255)
       cATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGcc                                                                                       <  2:790106/100‑1 (MQ=255)
       cATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCACCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGcc                                                                                       <  2:1362016/100‑1 (MQ=255)
        aTCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCca                                                                                      >  1:241348/1‑100 (MQ=255)
        aTCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCca                                                                                      <  2:892587/100‑1 (MQ=255)
        aTCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCca                                                                                      >  1:2486823/1‑100 (MQ=255)
         tCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcag                                                                                     <  1:1859444/100‑1 (MQ=255)
         tCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcag                                                                                     >  2:317329/1‑100 (MQ=255)
          ccTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagc                                                                                    >  1:1607303/1‑100 (MQ=255)
           cTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagca                                                                                   >  1:2202114/1‑100 (MQ=255)
            tGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcag                                                                                  >  1:11472/1‑100 (MQ=255)
            tGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcag                                                                                  <  1:2493963/100‑1 (MQ=255)
            tGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcag                                                                                  >  1:1469119/1‑100 (MQ=255)
            tGACCGGGAAATGTTCTTTCCTTTAAACCTCACTCTCCGCCATGTCGCCTTTCTCTTGCCACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcat                                                                                  <  2:75700/100‑2 (MQ=255)
                cgtgCAATGTTCTTTCCTTGAAACCTCAAGCTCGGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTGCCGAGCCTTTCTTCGCCAGCAGCata                                                                              <  2:2181570/97‑1 (MQ=255)
                  ggCAATGTTCTTTCCTTTAAACCTCAGTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATc                                                                            <  2:1149221/100‑1 (MQ=255)
                  ggCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATc                                                                            >  2:2024731/1‑100 (MQ=255)
                    cAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCca                                                                          <  2:1878951/100‑1 (MQ=255)
                     aaTGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCcat                                                                         >  1:847801/1‑100 (MQ=255)
                       tGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCcatca                                                                       >  2:705691/1‑100 (MQ=255)
                       tGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCcatca                                                                       <  2:544083/100‑1 (MQ=255)
                        gTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCcatcat                                                                      >  1:603117/1‑100 (MQ=255)
                         ttCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCatcatc                                                                     <  2:344125/100‑1 (MQ=255)
                         ttCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCatcatc                                                                     <  1:484988/100‑1 (MQ=255)
                            tttCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATcgcg                                                                  <  1:2222417/100‑1 (MQ=255)
                             ttCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGt                                                                 >  2:1279844/1‑100 (MQ=255)
                              tCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTc                                                                <  1:1844723/100‑1 (MQ=255)
                              tCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTc                                                                >  1:133267/1‑100 (MQ=255)
                               ccTTTAAACCTCGATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCa                                                               >  1:1078489/1‑100 (MQ=255)
                               ccTTTAAACCTCAATCTCCGCCATGTCGCCTTTCCCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCCTACCCATCATCGCGCCa                                                               >  2:1074615/1‑100 (MQ=255)
                                cTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCCTCATCGCGTCAg                                                              >  2:1502925/1‑100 (MQ=255)
                                cTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAg                                                              <  2:1130435/100‑1 (MQ=255)
                                cTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAg                                                              >  1:1116812/1‑100 (MQ=255)
                                  ttAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTa                                                            <  2:1077217/100‑1 (MQ=255)
                                  ttAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTa                                                            >  1:1417570/1‑100 (MQ=255)
                                      aCCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCt                                                        <  1:126667/100‑1 (MQ=255)
                                       ccTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                                                       >  2:283472/1‑100 (MQ=255)
                                       ccTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                                                       >  1:89422/1‑100 (MQ=255)
                                         tCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGAt                                                     <  2:632592/100‑1 (MQ=255)
                                         tCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCACTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGAt                                                     <  2:2376379/100‑1 (MQ=255)
                                         tCAATCGCCGCCATGTCGGCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGAt                                                     >  1:2199484/1‑100 (MQ=255)
                                           aaTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCCTCATCGCGTCAGTACGCTGATCg                                                   >  2:2588810/1‑100 (MQ=255)
                                           aaTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCg                                                   <  1:880840/100‑1 (MQ=255)
                                               tcCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCtt                                               >  2:544570/1‑100 (MQ=255)
                                               accGCCATGACGCCCCTCTCATCCAAACAGTTGCCGCGATCTCCCGGGCCTTTTTTCGACCGGAGCATCTCCATCCTCTCGTTAGTACGCTGATCGTCtt                                               <  2:2593075/99‑1 (MQ=255)
                                                ccGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtc                                              >  2:807509/1‑100 (MQ=255)
                                                 cGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtca                                             <  2:571210/100‑1 (MQ=255)
                                                 cGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtca                                             <  2:909992/100‑1 (MQ=255)
                                                 cGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtca                                             >  1:1641122/1‑100 (MQ=255)
                                                 cGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtca                                             <  1:853198/100‑1 (MQ=255)
                                                  gCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtcat                                            >  1:2030773/1‑100 (MQ=255)
                                                  gCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTAAGTACGCTGATCGTCTtcat                                            >  2:744820/1‑100 (MQ=255)
                                                   ccATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtcatc                                           <  2:1222572/100‑1 (MQ=255)
                                                   ccATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtcatc                                           <  2:1222565/100‑1 (MQ=255)
                                                     aTGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCCTcatcat                                         <  2:217431/100‑1 (MQ=255)
                                                      tGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCatcatc                                        <  2:1137015/100‑1 (MQ=255)
                                                      tGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCatcatc                                        <  1:821258/100‑1 (MQ=255)
                                                        tCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGa                                      >  2:1975109/1‑100 (MQ=255)
                                                         cGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGAt                                     >  1:2606134/1‑100 (MQ=255)
                                                         cGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGAt                                     >  2:363596/1‑100 (MQ=255)
                                                         cGCATTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTCTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGACCGTCTTCATCATCGAt                                     >  2:1077138/1‑100 (MQ=255)
                                                           ccTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAg                                   >  2:926637/1‑100 (MQ=255)
                                                           ccTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAg                                   >  1:244663/1‑100 (MQ=255)
                                                           ccTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAg                                   <  2:1237787/100‑1 (MQ=255)
                                                           ccTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGCCAGTACGCTGATCGTCTTCATCATCGATAg                                   >  2:926897/1‑100 (MQ=255)
                                                            cTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGt                                  >  1:2349838/1‑100 (MQ=255)
                                                                   ttGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGc                           >  2:2358779/1‑100 (MQ=255)
                                                                   ttGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGc                           <  2:213772/100‑1 (MQ=255)
                                                                    tGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCa                          <  2:1170984/100‑1 (MQ=255)
                                                                     gCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCAc                         <  1:1599465/100‑1 (MQ=255)
                                                                      ctaCCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCTTCTTCATCATCGATAGTCACCTGCAcc                        <  2:2581217/98‑1 (MQ=255)
                                                                      cAAGCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCAcc                        >  2:2382576/1‑100 (MQ=255)
                                                                      cAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCAcc                        <  1:673682/100‑1 (MQ=255)
                                                                       aaCCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCa                       <  1:1526870/100‑1 (MQ=255)
                                                                        aCCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAg                      >  2:456343/1‑100 (MQ=255)
                                                                         ccAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGa                     <  1:990843/100‑1 (MQ=255)
                                                                           aGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACg                   >  1:87857/1‑100 (MQ=255)
                                                                              tGCGGCGATCTTCCGTGCCTTTCTTCGCCATGAGCATATCGAACATCGGGTCAGTACGCGGAACATCTTCATCATCGATAGTCAACTGCACCAGACGGCg                <  2:2534211/100‑1 (MQ=255)
                                                                                cggcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCCGACGGCGAg              >  1:21291/1‑100 (MQ=255)
                                                                                cggcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAg              <  2:190222/100‑1 (MQ=255)
                                                                                cggcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAg              >  2:2313477/1‑100 (MQ=255)
                                                                                cggcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCACCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAg              <  1:774595/100‑1 (MQ=255)
                                                                                   cgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAgtgt           <  1:1897661/100‑1 (MQ=255)
                                                                                     aTCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTc         >  2:710236/1‑100 (MQ=255)
                                                                                      tCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGCTCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCg        <  2:1643397/100‑1 (MQ=255)
                                                                                      tCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCg        <  1:43514/100‑1 (MQ=255)
                                                                                       cTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCgg       >  1:2636672/1‑100 (MQ=255)
                                                                                         tCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGAt     >  2:2531291/1‑100 (MQ=255)
                                                                                          ccGCGCCTTCCTTGGCCAGCAGCATATCCATCAGCGCGTTCGTACGCTGATCGCCTTCTTCATCGATAGTCAACTGCACCAGGCGGCGAGTGTTCGGCTc    <  2:1423526/100‑1 (MQ=255)
                                                                                           cGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCa   >  2:1163301/1‑100 (MQ=255)
                                                                                           cGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCa   >  2:1584733/1‑100 (MQ=255)
                                                                                            gAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCaa  <  2:711372/100‑1 (MQ=255)
                                                                                                |                                                                                               
GCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAA  >  NC_000913/3173470‑3173661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑