Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,269,918 G→A intergenic (+316/+298) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9180GA98.5% 392.5 / NA 130intergenic (+316/+298)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (65/62);  total (67/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCT  >  NC_000913/3269822‑3270014
                                                                                                |                                                                                                
 aaTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCgn                                                                                              <  1:2096632/100‑2 (MQ=255)
 aaTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGt                                                                                              >  1:1168673/1‑100 (MQ=255)
 aaTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTCTTGGGTATAGTATCGt                                                                                              >  1:834515/1‑100 (MQ=255)
  aTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTg                                                                                             <  2:1562514/100‑1 (MQ=255)
  aTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTg                                                                                             >  1:70904/1‑100 (MQ=255)
  aTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTg                                                                                             <  2:552519/100‑1 (MQ=255)
  aTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGGTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                                             >  2:319453/1‑100 (MQ=255)
   tAAAGGGCACTCAACAACCTGAAACCCGCCGGAACGTTTTAGTACTTACATCTGGTAAAACTGACCTATAGTTCGCTTTCTTTTGGGTATAGTATCGTgg                                                                                            <  2:531297/100‑1 (MQ=25)
     aaGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGAc                                                                                          >  2:953408/1‑100 (MQ=255)
      aGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACa                                                                                         <  1:17050/100‑1 (MQ=255)
          cACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGtca                                                                                     >  1:2083970/1‑100 (MQ=255)
            cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGtcatt                                                                                   >  2:805578/1‑100 (MQ=255)
            cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTCGGGTATAGTATCGTGGACAGtcatt                                                                                   >  1:2460602/1‑100 (MQ=255)
            cTCAACAAACTGAAACCCGCCGGAACGGGTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTCTTGTGTGGAGTAGCGTCGACAGtcatt                                                                                   >  2:897715/1‑100 (MQ=18)
             tCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGtcattc                                                                                  <  1:1392/100‑1 (MQ=255)
              caacaaTCTGAACCCCGCCGGAGCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGtcattca                                                                                 <  2:558554/100‑1 (MQ=255)
              caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGtcattca                                                                                 >  2:229567/1‑100 (MQ=255)
               aacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGtcattcat                                                                                >  2:1287720/1‑100 (MQ=255)
               aacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGtcattcat                                                                                <  2:706167/100‑1 (MQ=255)
                acaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCGGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATc                                                                               >  2:1772110/1‑100 (MQ=255)
                  aaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCtt                                                                             >  1:437515/1‑100 (MQ=255)
                   aTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCttt                                                                            >  1:475480/1‑100 (MQ=255)
                    tCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTCTTGGGTATAGTATCGTGGACAGTCATTCATCTTTc                                                                           <  2:2531312/100‑1 (MQ=255)
                    tCTGAAACCCGCCGGAACGGCTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTc                                                                           <  1:2477154/100‑1 (MQ=255)
                     cTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCt                                                                          >  1:2274123/1‑100 (MQ=255)
                      tGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCCTGGACAGTCATTCATCTTTCTg                                                                         >  2:1557709/1‑100 (MQ=255)
                       gattcccGCCTGATCGGTTTATTACGTACATCAGGTAAAACTGTCCGATAAGCTGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGc                                                                        <  2:545517/96‑1 (MQ=17)
                        aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGcc                                                                       <  1:504365/100‑1 (MQ=255)
                          aCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGcccc                                                                     >  2:2159817/1‑100 (MQ=255)
                          aCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGcccc                                                                     <  1:2416763/100‑1 (MQ=255)
                             cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                                  <  2:2218353/100‑1 (MQ=255)
                             cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                                  >  1:1499979/1‑100 (MQ=255)
                             cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                                  <  2:2237655/100‑1 (MQ=255)
                             cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAACCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                                  <  2:2430061/100‑1 (MQ=255)
                               ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGGCAGTCATTCATCTTTCTGCCCCTCCaa                                                                >  2:1806457/1‑100 (MQ=255)
                                cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCaga                                                               >  2:1782407/1‑98 (MQ=255)
                                cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaa                                                               >  1:1875597/1‑100 (MQ=255)
                                 ggAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                              >  1:1058229/1‑100 (MQ=255)
                                  gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg                                                             <  1:1691460/100‑1 (MQ=255)
                                  gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTACCCCTCCAAAAg                                                             <  1:2277833/100‑1 (MQ=255)
                                   aaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGt                                                            <  1:117203/100‑1 (MQ=255)
                                    aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTa                                                           <  2:47679/100‑1 (MQ=255)
                                    aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTCTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTa                                                           >  1:2055674/1‑100 (MQ=255)
                                     cGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaa                                                          >  1:1541521/1‑100 (MQ=255)
                                      ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                         <  2:945181/100‑1 (MQ=255)
                                      ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                         >  2:1714141/1‑100 (MQ=255)
                                      ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTATGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                         >  2:2090274/1‑100 (MQ=255)
                                       gTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaaa                                                        >  1:575063/1‑100 (MQ=255)
                                        tttATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaaaa                                                       >  1:2065705/1‑100 (MQ=255)
                                           attaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTGCAAAAGTAAAGAcca                                                    >  2:484479/1‑99 (MQ=17)
                                           attaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAccc                                                    >  1:1107335/1‑100 (MQ=255)
                                             taCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACccgc                                                  >  1:1553679/1‑100 (MQ=255)
                                             taCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAATCGTGGACAGTCTTTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                                  >  2:2557929/1‑100 (MQ=14)
                                               cGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACccgccg                                                >  1:1656665/1‑100 (MQ=255)
                                                 tACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGCCAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGaa                                              >  1:647055/1‑100 (MQ=255)
                                                 tACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTGCAAGAGTAAACGCCCGCTGag                                              >  2:382855/1‑99 (MQ=14)
                                                 tACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGaa                                              >  2:2342580/1‑100 (MQ=255)
                                                    aTCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCg                                           >  1:2149658/1‑100 (MQ=255)
                                                      cAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCggg                                         >  1:406654/1‑100 (MQ=255)
                                                       aGGTAAAACTGAGCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGt                                        >  1:2270857/1‑100 (MQ=255)
                                                       aGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGt                                        >  1:961776/1‑100 (MQ=255)
                                                        ggTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGtt                                       >  1:814282/1‑100 (MQ=255)
                                                        ggTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGtt                                       >  1:674201/1‑100 (MQ=255)
                                                         gTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttt                                      <  2:1866159/100‑1 (MQ=255)
                                                           aaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttttt                                    <  2:1233367/100‑1 (MQ=25)
                                                           aaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttttt                                    <  2:2296968/100‑1 (MQ=25)
                                                            aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTa                                   <  1:1396747/100‑1 (MQ=18)
                                                             aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTAc                                  <  1:563179/100‑1 (MQ=11)
                                                             aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTAc                                  <  1:1737678/100‑1 (MQ=11)
                                                             aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGGTCTTAc                                  >  2:2254671/1‑100 (MQ=11)
                                                              aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTGATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGCTTTTTACg                                 <  2:2492181/100‑1 (MQ=11)
                                                              aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTAct                                 <  1:1952651/100‑2 (MQ=11)
                                                              aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTAct                                 <  1:1997305/100‑2 (MQ=11)
                                                              aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACg                                 <  1:1125108/100‑1 (MQ=21)
                                                               cTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTActt                                <  1:943956/100‑3 (MQ=11)
                                                               cTGACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTActt                                <  2:1358684/100‑3 (MQ=11)
                                                                 gACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaa                              >  1:1870778/1‑100 (MQ=21)
                                                                 gACCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCCTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTActttt                              <  2:1986711/100‑5 (MQ=11)
                                                                 gACCGATAAGCCGCTTTCTTTTGGGTATAGTATCCTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaa                              >  2:1038687/1‑100 (MQ=16)
                                                                  aCCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                             <  1:1178648/100‑1 (MQ=21)
                                                                  aCCGATAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                             <  2:398522/100‑1 (MQ=21)
                                                                       tAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAgg                        <  1:2331790/100‑1 (MQ=255)
                                                                       tAAGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAgg                        >  2:1115791/1‑100 (MQ=255)
                                                                         aGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg                      <  1:851796/100‑1 (MQ=255)
                                                                         aGCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg                      <  2:257560/100‑1 (MQ=255)
                                                                          gCCGCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCACCCCCAAAAGTAAAAACCCGCCGAAGCGGGATTTCAGGTAAAACAGGGGa                     >  1:1718004/1‑100 (MQ=11)
                                                                             gCTTTCTTTTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAc                  <  2:1041782/100‑1 (MQ=255)
                                                                               tttctttTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTg                >  2:734337/1‑100 (MQ=255)
                                                                               tttctttTGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTg                <  1:1244152/100‑1 (MQ=255)
                                                                                   ttttGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCg            <  2:246069/100‑1 (MQ=37)
                                                                                    tttGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa           <  2:1713728/100‑1 (MQ=255)
                                                                                      tGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATa         <  2:1108783/100‑1 (MQ=255)
                                                                                      tGGGTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATa         <  1:664278/100‑1 (MQ=255)
                                                                                       gggTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa        >  2:314788/1‑100 (MQ=255)
                                                                                        ggTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCCCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAATGACCGATAAg       >  1:814304/1‑100 (MQ=255)
                                                                                        ggTATAGTATCGTGGACAGTCATACATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGGGAAACTGACCGATAAg       >  2:2177190/1‑100 (MQ=11)
                                                                                         gTATAGTCTCGTGGCCAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCGGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc      >  2:266040/1‑100 (MQ=11)
                                                                                         gTATAGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc      >  2:1554569/1‑100 (MQ=255)
                                                                                          tataGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc     >  2:2268220/1‑100 (MQ=255)
                                                                                             aGTATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGct  >  1:1110725/1‑100 (MQ=255)
                                                                                                |                                                                                                
GAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCT  >  NC_000913/3269822‑3270014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: