BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	243,264	C→T	G14S (GGC→AGC)	fadE ←	acyl coenzyme A dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	243,264	0	C	T	100.0%	387.4 / NA	114	G14S (GGC→AGC)	fadE	acyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (51/63); total (51/63)

Only 100 of 114 total aligned reads displayed.

CGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCCGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTGTAGT  >  NC_000913/243169‑243360

cGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGc                                                                                              >  2:848300/1‑100 (MQ=255)

cGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGc                                                                                              <  1:475732/100‑1 (MQ=255)

cGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGc                                                                                              <  2:1015835/100‑1 (MQ=255)

cGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGc                                                                                              >  1:766614/1‑100 (MQ=255)

 gCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCa                                                                                             <  1:1989595/100‑1 (MQ=255)

 gCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCa                                                                                             <  1:661107/100‑1 (MQ=255)

  ccGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAg                                                                                            <  1:573076/100‑1 (MQ=255)

   cGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAgg                                                                                           <  1:1286486/100‑1 (MQ=255)

   cGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAgg                                                                                           <  1:297752/100‑1 (MQ=255)

   cGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAgg                                                                                           <  2:517220/100‑1 (MQ=255)

    gACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGa                                                                                          >  1:426998/1‑100 (MQ=255)

     aCCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGAc                                                                                         <  2:372640/100‑1 (MQ=255)

        acaGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAAc                                                                                      >  1:1517110/1‑100 (MQ=255)

        acaGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAAc                                                                                      >  1:2645917/1‑100 (MQ=255)

         caGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAAcc                                                                                     >  1:2030240/1‑100 (MQ=255)

           gACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGt                                                                                   >  1:818072/1‑100 (MQ=255)

           gACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGt                                                                                   <  2:2388422/100‑1 (MQ=255)

            aCCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTa                                                                                  >  1:2449642/1‑100 (MQ=255)

              cAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGc                                                                                <  2:66600/100‑1 (MQ=255)

                 cAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCgag                                                                             <  1:522456/100‑1 (MQ=255)

                    cGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAAt                                                                          >  1:1322461/1‑100 (MQ=255)

                    cGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAAt                                                                          <  2:2024457/100‑1 (MQ=255)

                    cGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAAt                                                                          <  1:228808/100‑1 (MQ=255)

                     gCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATa                                                                         >  1:1757039/1‑100 (MQ=255)

                     gCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATa                                                                         <  1:2524142/100‑1 (MQ=255)

                      ccGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATAc                                                                        >  2:395763/1‑100 (MQ=255)

                      ccGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATAc                                                                        <  1:419104/100‑1 (MQ=255)

                       cGAGGGTGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGGGATAGACCAACGCTCTGAGCAGGACAATCGCAGCGAAAATACt                                                                       >  2:1205489/1‑100 (MQ=255)

                       cGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACt                                                                       <  1:782312/100‑1 (MQ=255)

                        gAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTc                                                                      >  2:1525688/1‑100 (MQ=255)

                        gAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTc                                                                      >  1:1899764/1‑100 (MQ=255)

                         aGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCa                                                                     <  2:1268458/100‑1 (MQ=255)

                            gcggcTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCaaaa                                                                  >  1:2609886/1‑100 (MQ=255)

                             cggcTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAat                                                                 <  1:1509200/100‑1 (MQ=255)

                             cggcTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAat                                                                 >  1:2463404/1‑100 (MQ=255)

                             cggcTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATACAACAACGCGCTGAGCAGGACAACCGTCGCGAGAATAGTCAAAat                                                                 >  1:2427513/1‑100 (MQ=255)

                               gcTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAatca                                                               >  1:2258029/1‑100 (MQ=255)

                                  gTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAAtcatca                                                            <  1:233295/100‑1 (MQ=255)

                                  gTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAAtcatca                                                            >  1:1990240/1‑100 (MQ=255)

                                  gTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAAtcatca                                                            <  1:2376892/100‑1 (MQ=255)

                                   tCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGCGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATcatcat                                                           >  1:2550917/1‑100 (MQ=255)

                                     cAGGCGAGCAAAATCAGACTGCTGATAAAAAAACTCACGCGGTGATAGAACAACGCGCTAAGCCGGACAACCGTAGCGAGAATACTCAAAATCATCATaa                                                         >  1:761352/1‑100 (MQ=255)

                                      aGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAAc                                                        <  2:2561214/100‑1 (MQ=255)

                                       ggCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACg                                                       >  2:1827367/1‑100 (MQ=255)

                                       ggCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACg                                                       >  1:226807/1‑100 (MQ=255)

                                       ggCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACg                                                       <  2:1847612/100‑1 (MQ=255)

                                            gCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAg                                                  <  1:958032/100‑1 (MQ=255)

                                            gCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAg                                                  >  1:2147435/1‑100 (MQ=255)

                                            gCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAg                                                  <  2:42691/100‑1 (MQ=255)

                                                    aGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTAc                                          >  1:664465/1‑100 (MQ=255)

                                                      aCTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACtt                                        <  2:2115516/100‑1 (MQ=255)

                                                        tgctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACCACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGt                                      <  2:748630/100‑1 (MQ=255)

                                                         tctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTa                                     <  2:1816261/99‑1 (MQ=255)

                                                         gctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTa                                     <  1:265774/100‑1 (MQ=255)

                                                          ctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTag                                    >  1:770122/1‑100 (MQ=255)

                                                          ctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTaa                                    >  1:1840768/1‑99 (MQ=255)

                                                           tgATAAATAAGCTCGCGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTagg                                   >  2:1306906/1‑100 (MQ=255)

                                                           tgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTagg                                   <  2:1621713/100‑1 (MQ=255)

                                                              taaataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCGAAATCATCATAACGAAAAGCCCCTTACTTGTaggagg                                >  1:1666549/1‑100 (MQ=255)

                                                              taaataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTaggagg                                <  1:691136/100‑1 (MQ=255)

                                                              taaataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTaggagg                                <  2:1178144/100‑1 (MQ=255)

                                                              taaataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTaggagg                                >  2:689407/1‑100 (MQ=255)

                                                              taaataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTAATTGTaggagg                                <  2:1701494/100‑1 (MQ=255)

                                                                  taaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTg                            <  1:2232760/100‑1 (MQ=255)

                                                                   aaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGa                           <  1:1465349/100‑1 (MQ=255)

                                                                    aGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGAc                          <  1:1977795/100‑1 (MQ=255)

                                                                    aGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGAc                          >  1:2179135/1‑100 (MQ=255)

                                                                    aGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGAc                          <  2:704725/100‑1 (MQ=255)

                                                                      cTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCa                        <  2:2311251/100‑1 (MQ=255)

                                                                        cACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGCATACTCAAAATAATCATAACGAAAAGCCCATTACTTGTAGGAGGACTGACCACt                      >  1:2271821/1‑100 (MQ=255)

                                                                        cACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAACTCATCATAACGAAAAGCGCCTTACTGGTAGGAGGTCTGACCCCt                      >  2:498608/1‑100 (MQ=255)

                                                                        cACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACt                      <  2:1118410/100‑1 (MQ=255)

                                                                         aCGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACtt                     >  2:1269552/1‑100 (MQ=255)

                                                                         aCGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCGTACTTGTAGGAGGTCTGACCACtt                     >  2:1431288/1‑100 (MQ=255)

                                                                          cgcgGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACGTGTAGGAGGTCTGACCACTtg                    <  2:101255/100‑1 (MQ=255)

                                                                            cgGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGAAGGAGGTCTGACCACTtgtg                  >  1:546244/1‑100 (MQ=255)

                                                                             ggTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAGAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtga                 <  2:2459018/100‑1 (MQ=255)

                                                                              gTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgat                <  1:923913/100‑1 (MQ=255)

                                                                              gTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgat                >  1:2205856/1‑100 (MQ=255)

                                                                              gTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgat                <  1:2158066/100‑1 (MQ=255)

                                                                              gTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgat                <  2:263106/100‑1 (MQ=255)

                                                                               tGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgatg               >  1:953146/1‑100 (MQ=255)

                                                                                gATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgatga              <  2:850025/100‑1 (MQ=255)

                                                                                gATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgatga              >  2:2066346/1‑100 (MQ=255)

                                                                                gATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgatga              <  2:1466022/100‑1 (MQ=255)

                                                                                gATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgatga              >  1:660117/1‑100 (MQ=255)

                                                                                  tAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGata            <  1:811290/100‑1 (MQ=255)

                                                                                  tAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGata            <  2:437996/100‑1 (MQ=255)

                                                                                    gAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATg          >  1:809087/1‑100 (MQ=255)

                                                                                     aacggcgcgCTGAGCAGGCCAACCGTAGCGAGAATACTCAAAATCATCATAGCGAAAAGCCCCTTACTTGTAGGAGGTCTGACCAATTGTGATGATATgg         <  2:2172526/95‑1 (MQ=255)

                                                                                     aacaacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATgg         <  1:2127863/100‑1 (MQ=255)

                                                                                     aacaacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATgg         >  1:1410694/1‑100 (MQ=255)

                                                                                      acaacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGt        <  1:1095526/100‑1 (MQ=255)

                                                                                      acaacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGt        <  1:1096533/100‑1 (MQ=255)

                                                                                      acaacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGt        <  1:35504/100‑1 (MQ=255)

                                                                                       caacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGACAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGtt       <  2:495125/100‑1 (MQ=255)

                                                                                        aacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTg      <  1:1677824/100‑1 (MQ=255)

                                                                                        aacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTg      <  1:1653088/100‑1 (MQ=255)

                                                                                         acGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTGt     <  2:724729/100‑1 (MQ=255)

                                                                                          cgcgCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTGTa    >  1:1899927/1‑100 (MQ=255)

CGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCCGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTGTAGT  >  NC_000913/243169‑243360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`