BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,928,131	T→C	V326A (GTC→GCC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,928,131	0	T	C	99.1%	382.7 / ‑4.6	113	V326A (GTC→GCC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (61/50); total (62/51)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 116 total aligned reads displayed.

GTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCA  >  NC_000913/3928035‑3928225

gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGcccc                                                                                             <  2:2541651/100‑1 (MQ=255)

gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGcccc                                                                                             <  2:1565967/100‑1 (MQ=255)

   tCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTtc                                                                                          >  2:1564/1‑100 (MQ=255)

   tCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTtc                                                                                          <  1:192014/100‑1 (MQ=255)

     ggCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGGGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTtctc                                                                                        <  2:1183445/100‑1 (MQ=255)

     ggCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTtctc                                                                                        >  2:96964/1‑100 (MQ=255)

     ggCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTtctc                                                                                        <  2:2292550/100‑1 (MQ=255)

        cAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTctgc                                                                                     <  1:668734/100‑1 (MQ=255)

          gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTctgctg                                                                                   <  1:2593312/100‑1 (MQ=255)

           tctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCCGTGTGGAGTATGGCCAGATGCCGTTCGCGCGAGCGCCCCTTCTctgctgc                                                                                  <  2:1591884/100‑2 (MQ=255)

            ctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCCGta                                                                                 >  2:1084171/1‑100 (MQ=255)

               ntttGACTATGCTGCTGCTGCAACTGCCACATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGtaata                                                                              >  1:2329603/2‑100 (MQ=255)

               gTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGtaata                                                                              >  1:2330922/1‑100 (MQ=255)

                tttGACTATGCTGCTGCTGCAACTGCCGCATATCGTCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGCGAGCGGCCCTTCTGTGCTGtaataa                                                                             >  2:779387/1‑100 (MQ=255)

                  tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAAtt                                                                           <  1:2580182/100‑1 (MQ=255)

                  tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAAtt                                                                           >  1:1786649/1‑100 (MQ=255)

                   gACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTGTCTGCTGTAATAAttt                                                                          <  2:146291/100‑1 (MQ=255)

                   gACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAAttt                                                                          <  2:2039921/100‑1 (MQ=255)

                     cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTAt                                                                        >  1:906551/1‑100 (MQ=255)

                     cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTAt                                                                        >  2:2453256/1‑100 (MQ=255)

                      tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATc                                                                       <  1:498912/100‑1 (MQ=255)

                       attctgctgctgcAACTTCCGCATATCGGCCAGGTTCAGTGTGGATTATGGCCAGCTGCTGTTCGCGAGATCGCCCCTTCTCTGCTGTAATAATTTATcg                                                                      <  1:2186052/97‑1 (MQ=255)

                        tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATcgc                                                                     >  1:2013787/1‑100 (MQ=255)

                          ctgctgctgcAACTGCCGCATATTGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATcgccg                                                                   <  2:419374/100‑1 (MQ=255)

                          ctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATcgccg                                                                   >  1:1684121/1‑100 (MQ=255)

                            gctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCgccgcc                                                                 >  1:468335/1‑100 (MQ=255)

                              tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAg                                                               <  2:727273/100‑1 (MQ=255)

                               gctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                                              >  1:528906/1‑100 (MQ=255)

                               gctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTAGGGCCAGCTGCTGTTCGCGAGATCGCCCCTTCTCTGCGGTAATAATTTATCGCCGCCAGc                                                              >  1:529158/1‑100 (MQ=255)

                                ctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCg                                                             >  1:1511106/1‑100 (MQ=255)

                                   cAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCt                                                          <  2:1210367/100‑1 (MQ=255)

                                   cAACTGCCGCATATCGGCCAGGTTCAGTGTGAAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCt                                                          >  1:2115234/1‑100 (MQ=255)

                                    aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                                         >  1:18164/1‑100 (MQ=255)

                                    aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                                         <  2:401059/100‑1 (MQ=255)

                                    aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                                         <  1:685773/100‑1 (MQ=255)

                                      cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                                       <  1:1519084/100‑1 (MQ=255)

                                       tGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                                      <  1:462675/100‑1 (MQ=255)

                                        gCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCa                                                     >  1:2450351/1‑100 (MQ=255)

                                        gCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCa                                                     <  1:379184/100‑1 (MQ=255)

                                        gCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCa                                                     >  2:1942003/1‑100 (MQ=255)

                                          cGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAgg                                                   >  2:305292/1‑100 (MQ=255)

                                          cGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAgg                                                   >  1:170743/1‑100 (MQ=255)

                                            cATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcg                                                 <  1:685302/100‑1 (MQ=255)

                                             atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcgg                                                >  2:2336482/1‑100 (MQ=255)

                                              tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                                               <  1:1375979/100‑1 (MQ=255)

                                              tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                                               >  1:602648/1‑100 (MQ=255)

                                               atCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCt                                              >  1:1349068/1‑100 (MQ=255)

                                               atCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCt                                              >  2:1565500/1‑100 (MQ=255)

                                                tCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                                             >  2:1665654/1‑100 (MQ=255)

                                                 cGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCCGCGTCTGAGCAGGCGGCTTc                                            >  2:2372090/1‑100 (MQ=255)

                                                 cGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTc                                            >  2:1434652/1‑100 (MQ=255)

                                                 cGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTc                                            <  1:2499665/100‑1 (MQ=255)

                                                  ggCTAGGTTCAGTGTGGAGTATGGCCAGCTGCTTTTCGCGGGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCg                                           <  2:1649359/100‑1 (MQ=255)

                                                  ggCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCg                                           >  2:1303276/1‑100 (MQ=255)

                                                   gCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                                          <  2:1563000/100‑1 (MQ=255)

                                                   gCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                                          <  2:621410/100‑1 (MQ=255)

                                                   gCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                                          <  1:690991/100‑1 (MQ=255)

                                                    ccAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                                         <  2:818221/100‑1 (MQ=255)

                                                      aGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATc                                       <  1:1585/100‑1 (MQ=255)

                                                       ggTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATcc                                      >  2:1901101/1‑100 (MQ=255)

                                                        gTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATccc                                     <  2:1310937/100‑1 (MQ=255)

                                                         ttCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCg                                    <  1:1109778/100‑1 (MQ=255)

                                                          tCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCgg                                   >  2:526172/1‑100 (MQ=255)

                                                            aGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTa                                 <  1:109576/100‑1 (MQ=255)

                                                            aGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTa                                 <  1:2271690/100‑1 (MQ=255)

                                                               gtgGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCa                              <  1:411347/100‑1 (MQ=255)

                                                               gtgGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCa                              >  2:158883/1‑100 (MQ=255)

                                                               gtgGAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCa                              >  2:2149673/1‑100 (MQ=255)

                                                                 ggAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCTGCTTCGCATCCCGGTATCaac                            >  2:714437/1‑99 (MQ=255)

                                                                 ggAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaaa                            >  1:1102189/1‑100 (MQ=255)

                                                                 ggAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaaa                            >  2:1861255/1‑100 (MQ=255)

                                                                  gAGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAg                           >  1:2400026/1‑100 (MQ=255)

                                                                   aGTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgc                          >  2:2306210/1‑100 (MQ=255)

                                                                    gTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcg                         <  1:1579752/100‑1 (MQ=255)

                                                                    gTATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcg                         >  2:2025818/1‑100 (MQ=255)

                                                                     tATGGCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcgc                        <  1:263256/100‑1 (MQ=255)

                                                                      aTGGCCAGCTGCTGTTCGCGTGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGcc                       <  1:2477402/100‑1 (MQ=255)

                                                                        ggCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAg                     >  1:289145/1‑100 (MQ=255)

                                                                        ggCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAg                     <  2:2297403/100‑1 (MQ=255)

                                                                         gCCAGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGa                    <  1:758069/100‑1 (MQ=255)

                                                                            aGCTGCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGatat                 >  2:1956710/1‑100 (MQ=255)

                                                                            aGCTCCTGTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCTGCGTCTGAGCAGGCGGCTTCGTATCCCGGTATCAAAGCGCCAGatat                 <  1:2368390/100‑1 (MQ=255)

                                                                             nctgctgTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATg                >  1:1068081/2‑100 (MQ=255)

                                                                               tgctgTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGAt              >  1:1762713/1‑100 (MQ=255)

                                                                                gctgTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATc             >  1:1961104/1‑100 (MQ=255)

                                                                                gctgTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATc             >  1:547446/1‑100 (MQ=255)

                                                                                gctgTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCACACCCCGGTATCAAAGCGCCAGATATGATc             >  2:1788111/1‑100 (MQ=255)

                                                                                gctgTTCGCGAGAGCGCCCCTTCTCTGCCGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATc             <  1:2398528/100‑1 (MQ=255)

                                                                                 ctgTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCg            <  2:1583005/100‑1 (MQ=255)

                                                                                   gTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATGCCGGTATCAAAGCGCCAGATATGATCgca          >  1:1142545/1‑98 (MQ=255)

                                                                                   gTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaa          >  1:1980169/1‑100 (MQ=255)

                                                                                   gTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaa          >  1:1216989/1‑100 (MQ=255)

                                                                                   gTTCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaa          >  1:406834/1‑100 (MQ=255)

                                                                                    ttCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaaa         <  1:644386/100‑1 (MQ=255)

                                                                                     tCGCGAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaaaa        <  1:789698/100‑1 (MQ=255)

                                                                                      cgcgAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAAt       >  1:565236/1‑100 (MQ=255)

                                                                                      cgcgAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAAt       >  1:1254141/1‑100 (MQ=255)

                                                                                       gcgAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATg      >  2:2285948/1‑100 (MQ=255)

                                                                                       gcgAGAGCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATg      >  1:675318/1‑100 (MQ=255)

                                                                                           gagCGCCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCa  <  1:1739872/100‑1 (MQ=255)

GTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCA  >  NC_000913/3928035‑3928225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`