Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,135,739 T→C V635V (GTT→GTC)  katG → catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,135,7390TC98.0% 337.3 / ‑4.6 102V635V (GTT→GTC) katGcatalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (51/49);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGAC  >  NC_000913/4135642‑4135835
                                                                                                 |                                                                                                
cGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCgg                                                                                                <  1:1692888/100‑1 (MQ=255)
cGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCgg                                                                                                <  2:1170763/100‑1 (MQ=255)
cGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCgg                                                                                                <  2:2105739/100‑1 (MQ=255)
 gCTGACCGCGCCGGAAATGACTGCGCTGGTGGGTGGCATGCGTGTACTGGGTGCCAACTTCGACGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGc                                                                                               >  1:2490724/1‑100 (MQ=255)
 gCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGc                                                                                               <  1:244805/100‑1 (MQ=255)
  cTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCg                                                                                              >  1:44206/1‑100 (MQ=255)
      ccGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAtt                                                                                          <  1:475443/100‑1 (MQ=255)
      ccGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAtt                                                                                          >  2:1173924/1‑100 (MQ=255)
      ccGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAtt                                                                                          >  2:1786344/1‑100 (MQ=255)
      ccGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAtt                                                                                          <  1:2203569/100‑1 (MQ=255)
        gcgcCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGa                                                                                        <  1:1277262/100‑1 (MQ=255)
         cgcCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAg                                                                                       <  1:1697683/100‑1 (MQ=255)
          gcCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGTAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGc                                                                                      <  1:2306662/100‑1 (MQ=255)
          gcCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGc                                                                                      <  1:907608/100‑1 (MQ=255)
          gcCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGc                                                                                      >  2:1622407/1‑100 (MQ=255)
           ccGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGCGCa                                                                                     >  1:1190716/1‑100 (MQ=255)
           ccGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCa                                                                                     >  1:1089284/1‑100 (MQ=255)
             ggAAATGACTGCGCTGGTGGGGGGCATGCGTGTACTGGGTGCCAACTTAGAAAAAAGAAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAAt                                                                                   <  2:2251330/100‑1 (MQ=255)
             ggAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAAt                                                                                   >  1:2442722/1‑100 (MQ=255)
             ggAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAAt                                                                                   >  1:1089908/1‑100 (MQ=255)
               aaaTGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGa                                                                                 <  2:1249487/100‑1 (MQ=255)
               aaaTGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGa                                                                                 <  2:1249202/100‑1 (MQ=255)
                  tGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGGTGGCAGCAAAAACGGCGTCTTCACTGACGGCGCCGGCGTATTGAGCAATGActt                                                                              >  2:1440744/1‑100 (MQ=255)
                  tGACTGCGCTGGTGCGCGGCATGCGTGTGCTGAGTGCATACTTCGATGGCAGCAAAAACGGCTTCTTCACTGACCGCGTCGGCGCATTGAGCAATGActt                                                                              >  2:1555758/1‑100 (MQ=255)
                       gcgcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCg                                                                         <  1:1973956/100‑1 (MQ=255)
                       gcgcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCg                                                                         <  1:766225/100‑1 (MQ=255)
                       gcgcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACGTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCg                                                                         <  1:766298/100‑1 (MQ=255)
                        cgcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCCCTGACCGCGTCGGCGTATGGAGCAATGACTTCTTCGt                                                                        <  2:699418/100‑1 (MQ=255)
                        cgcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGt                                                                        >  2:1767139/1‑100 (MQ=255)
                        cgcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGt                                                                        >  2:2008527/1‑100 (MQ=255)
                          cTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGCCCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGa                                                                      >  2:2559525/1‑100 (MQ=255)
                                 gcggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCt                                                               >  1:1136899/1‑100 (MQ=255)
                                  cggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTg                                                              <  2:31744/100‑1 (MQ=255)
                                  cggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTg                                                              <  1:811714/100‑1 (MQ=255)
                                  cggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTg                                                              <  1:1541634/100‑1 (MQ=255)
                                   ggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTgg                                                             <  1:1571014/100‑1 (MQ=255)
                                   ggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTgg                                                             <  2:1506726/100‑1 (MQ=255)
                                    gcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGa                                                            >  2:1501179/1‑100 (MQ=255)
                                    gcATGCGTGTACTGGGTGCCAACTTCGATCGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGa                                                            >  2:536913/1‑100 (MQ=255)
                                     cATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGat                                                           >  2:584383/1‑100 (MQ=255)
                                      aTGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGata                                                          <  1:2481009/100‑1 (MQ=255)
                                      aTGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGata                                                          <  2:1330758/100‑1 (MQ=255)
                                       tGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGatat                                                         >  2:2243128/1‑100 (MQ=255)
                                       tGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGatat                                                         >  1:931219/1‑100 (MQ=255)
                                       tGCGTGTACTGGGAGCCAACTTCGATGGCAGCAACAACGGCGTCTTCACTGACCGCGTCGGCGTATTCAGCAATGACTTCTTCGTGAACTTGCTGGaatt                                                         >  2:419604/1‑97 (MQ=255)
                                        gCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATg                                                        <  2:2384889/100‑1 (MQ=255)
                                         cGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGc                                                       >  1:1996473/1‑100 (MQ=255)
                                          gtgtACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCg                                                      <  1:302957/100‑1 (MQ=255)
                                          gtgtACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCg                                                      >  2:1020404/1‑100 (MQ=255)
                                           tgtACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGt                                                     >  1:178078/1‑100 (MQ=255)
                                           tgtACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGt                                                     >  2:301890/1‑100 (MQ=255)
                                             tACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTa                                                   >  1:516387/1‑100 (MQ=255)
                                               cTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACg                                                 >  1:927892/1‑100 (MQ=255)
                                               cTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACg                                                 >  1:928663/1‑100 (MQ=255)
                                                tGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGa                                                <  2:819077/100‑1 (MQ=255)
                                                   gTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATCTGCGTTACGAGTg                                             <  1:2323051/100‑1 (MQ=255)
                                                   gTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTg                                             >  2:1693448/1‑100 (MQ=255)
                                                     gCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGa                                           <  1:2281651/100‑1 (MQ=255)
                                                     gCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGa                                           >  1:1397649/1‑100 (MQ=255)
                                                      ccAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGaa                                          <  1:1743598/100‑1 (MQ=255)
                                                       cAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTATTCGTGAACTTGCTGGATATGCGTTACGAGTGGaaa                                         >  1:2489941/1‑100 (MQ=255)
                                                        aaCTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAg                                        <  1:547414/100‑1 (MQ=255)
                                                         aCTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGc                                       <  1:1063417/100‑1 (MQ=255)
                                                            tCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACCTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgac                                    >  2:795066/1‑100 (MQ=255)
                                                             cGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgacc                                   <  2:239897/100‑1 (MQ=255)
                                                              gATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAACGcgaacg                                  >  2:2342217/1‑100 (MQ=255)
                                                              gATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgaccg                                  >  1:737226/1‑100 (MQ=255)
                                                              gATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgaccg                                  >  1:509278/1‑100 (MQ=255)
                                                                tGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgaccgac                                >  1:2180147/1‑100 (MQ=255)
                                                                  gcagcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACcgacga                              >  2:1149077/1‑100 (MQ=255)
                                                                  gcagcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACcgacga                              <  2:1319432/100‑1 (MQ=255)
                                                                   cagcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACcgacgac                             >  1:1297913/1‑99 (MQ=255)
                                                                   cagcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGaa                             >  1:1089556/1‑100 (MQ=255)
                                                                    agcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAAt                            <  1:2425264/100‑1 (MQ=255)
                                                                       aaaaaCGGCGTCTTCACTGACCGCGTCGGCGTATTTAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACAAATACAAAGCCCCCTAAGAATCGa                         <  2:408152/100‑1 (MQ=255)
                                                                       aaaaaCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGa                         <  2:1604455/100‑1 (MQ=255)
                                                                        aaaaCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCgga                        <  2:912511/100‑3 (MQ=255)
                                                                          aaCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGTGTTACGAGTGGAAAGCGACCGACGAATCGAAag                      <  1:2035465/100‑1 (MQ=255)
                                                                          aaCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAag                      <  2:2105499/100‑1 (MQ=255)
                                                                           aCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAaga                     <  1:2349633/100‑1 (MQ=255)
                                                                             ggCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGc                   <  2:428872/100‑1 (MQ=255)
                                                                             ggCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGc                   <  2:1498429/100‑1 (MQ=255)
                                                                                gTCTTCACTGACCTCGTCGGCGTATTGACCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATAGAAAGAGCTGt                >  1:2325710/1‑100 (MQ=255)
                                                                                gTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGt                >  2:52012/1‑100 (MQ=255)
                                                                                 tCTTCACTTACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGtt               <  2:1729884/100‑1 (MQ=255)
                                                                                 tCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGtt               >  2:2543065/1‑100 (MQ=255)
                                                                                  cTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTc              >  2:1747642/1‑100 (MQ=255)
                                                                                  cTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTc              <  1:125760/100‑1 (MQ=255)
                                                                                    tCACTGAGCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGCTATGCGTTAGGAGAGCAAAACGACCGACGAATCGTAAGAGCTAGTCGa            >  2:1062061/1‑100 (MQ=255)
                                                                                      aCTGAACGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGGTATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAg          >  2:1241550/1‑100 (MQ=255)
                                                                                       cTGACCTTGTTTTCGTATTGAGCAATGACGTCTTCGTGAACTTGCTGGATATGCGTTCCGAGTGGAAAGCGTCCGACGAATCGAAAGAGCTGTTCGAAgg         <  2:2047217/100‑1 (MQ=255)
                                                                                       cTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAgg         >  1:711821/1‑100 (MQ=255)
                                                                                       cTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAgg         >  1:35902/1‑100 (MQ=255)
                                                                                        tGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGc        >  1:405114/1‑100 (MQ=255)
                                                                                        tGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGc        >  1:405318/1‑100 (MQ=255)
                                                                                        tGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGc        <  1:2124515/100‑1 (MQ=255)
                                                                                         gACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGcc       >  1:1158320/1‑100 (MQ=255)
                                                                                          aCCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccg      <  1:5199/100‑1 (MQ=255)
                                                                                             gcgTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtga   >  1:1507222/1‑100 (MQ=255)
                                                                                              cgTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtgac  >  1:2122552/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGAC  >  NC_000913/4135642‑4135835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑