Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,451,758	A→G	Q234R (CAG→CGG)	ytfR →	putative sugar ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,451,758	0	A	G	100.0%	341.5 / NA	98	Q234R (CAG→CGG)	ytfR	putative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/53);  total (45/53)

CTCACTTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGCC  >  NC_000913/4451662‑4451852                                                                                                 |                                                                                               ctcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGAt                                                                                             >  1:1841797/1‑100 (MQ=255) ctcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGAt                                                                                             >  1:2479261/1‑100 (MQ=255) ctcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGAt                                                                                             >  1:290066/1‑100 (MQ=255)  tcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATc                                                                                            <  2:1770777/100‑1 (MQ=255)  tcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATc                                                                                            <  2:1704396/100‑1 (MQ=255)  tcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATc                                                                                            >  2:1532797/1‑100 (MQ=255)  tcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATc                                                                                            <  1:560908/100‑1 (MQ=255)   cactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCg                                                                                           >  1:944088/1‑100 (MQ=255)   cactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCg                                                                                           >  1:398425/1‑100 (MQ=255)   cactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCg                                                                                           >  2:114212/1‑100 (MQ=255)   cactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCg                                                                                           <  2:935756/100‑1 (MQ=255)    actTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGa                                                                                          >  2:2314976/1‑100 (MQ=255)    actTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGGCGGGAAACGTGCGAGCTACCGCGGATCGa                                                                                          >  1:2629499/1‑100 (MQ=255)     ctTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGCGCTACCGCGGATCGaa                                                                                         >  1:2175057/1‑100 (MQ=255)      tttCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAAc                                                                                        <  1:422075/100‑1 (MQ=255)      tttCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAAc                                                                                        <  1:2596017/100‑1 (MQ=255)      tttCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAAc                                                                                        <  2:911707/100‑1 (MQ=255)       ttCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACt                                                                                       <  1:648943/100‑1 (MQ=255)       ttCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACt                                                                                       >  2:1431102/1‑100 (MQ=255)       ttCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACt                                                                                       >  1:1238091/1‑100 (MQ=255)        tctcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTg                                                                                      <  1:1696044/100‑1 (MQ=255)        tctcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTGTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTg                                                                                      <  1:72520/100‑1 (MQ=255)         ctcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTgg                                                                                     >  1:1632269/1‑100 (MQ=255)         ctcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTgg                                                                                     <  1:90190/100‑1 (MQ=255)          tcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGt                                                                                    <  2:1812053/100‑1 (MQ=255)          tcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGt                                                                                    <  1:386815/100‑1 (MQ=255)          tcGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGt                                                                                    <  1:2172216/100‑1 (MQ=255)             aTCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGGAACGCCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTaaa                                                                                 >  1:2295967/1‑100 (MQ=255)                atgTCTATAAGCTCGGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGGAGGCTGTCGGGAAACGTGCGAGCTCCCGCGGATCGAACTGGTAAAAat                                                                              <  2:2074109/98‑1 (MQ=255)                 ggTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatg                                                                             >  1:2212754/1‑100 (MQ=255)                 ggTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatg                                                                             <  2:1587205/100‑1 (MQ=255)                 ggTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatg                                                                             <  2:490796/100‑1 (MQ=255)                 ggTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatg                                                                             >  2:2466701/1‑100 (MQ=255)                 ggTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatg                                                                             >  2:72211/1‑100 (MQ=255)                   tCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatgat                                                                           <  2:1949957/100‑1 (MQ=255)                   tCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAatgat                                                                           <  1:335829/100‑1 (MQ=255)                          ggTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTCCCGCGGAGCGAACTGGTAAAAATGATGCTcggg                                                                    >  2:2045573/1‑100 (MQ=255)                          ggTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTgggg                                                                    <  2:1580319/100‑1 (MQ=255)                              cgccaTCGCATCACCGTCCGACGCAACGGCAGGCTCTTAGGCTGTCGGGAAACCTCCCAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGcgcg                                                                <  1:2170054/96‑1 (MQ=255)                              aGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGcgcg                                                                <  1:1395978/100‑1 (MQ=255)                               gCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGa                                                               >  1:848161/1‑100 (MQ=255)                               gCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGa                                                               >  2:1092229/1‑100 (MQ=255)                                 ggtCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGc                                                             <  1:1004732/98‑1 (MQ=255)                                   tCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTg                                                           <  1:287481/100‑1 (MQ=255)                                     ggATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGa                                                         >  2:1021550/1‑100 (MQ=255)                                     ggATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGa                                                         <  2:415763/100‑1 (MQ=255)                                     ggATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGa                                                         >  1:1839163/1‑100 (MQ=255)                                     ggATCACCATCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGa                                                         >  2:2487783/1‑100 (MQ=255)                                      gATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGAt                                                        >  2:1283560/1‑100 (MQ=255)                                        tCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATAc                                                      <  2:1314403/100‑1 (MQ=255)                                         cACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATAcc                                                     <  1:478316/100‑1 (MQ=255)                                          aCCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATAccc                                                    <  2:274550/100‑1 (MQ=255)                                           ccGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCa                                                   >  1:2318550/1‑100 (MQ=255)                                            cGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCAc                                                  <  1:1181357/100‑1 (MQ=255)                                              tCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCAcgc                                                <  1:1354153/100‑1 (MQ=255)                                               cTTACGCAACGGCAGTTTCGTCGGCTGTCGGGAAACGTGCGAGCTACCGCGGCACGACCTGGTAAAAATCATGCTGGGGCGCGCGCTGGATACCCAcgcg                                               >  2:1256641/1‑100 (MQ=255)                                                   cGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTAc                                           >  1:411784/1‑100 (MQ=255)                                                   cGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTAc                                           >  1:726186/1‑100 (MQ=255)                                                     cAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAg                                         <  1:962797/100‑1 (MQ=255)                                                     cAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAg                                         <  1:277948/100‑1 (MQ=255)                                                       aCGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCg                                       <  2:911705/100‑1 (MQ=255)                                                             gTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCgg                                 >  2:460566/1‑100 (MQ=255)                                                             gTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCgg                                 >  1:1077069/1‑100 (MQ=255)                                                             gTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCgg                                 <  1:1466059/100‑1 (MQ=255)                                                              tttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCggg                                <  1:2544584/100‑1 (MQ=255)                                                              tttCGCCGGGTGTCGGGAAACGTGCGAGATAACGGGGATCGAAATGGTAAAAATGATGCCGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCggg                                <  2:764845/100‑1 (MQ=255)                                                                tCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCg                              >  1:2423373/1‑100 (MQ=255)                                                                 cGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGa                             <  1:767369/100‑1 (MQ=255)                                                                 cGTAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGa                             <  2:2289915/100‑1 (MQ=255)                                                                   tAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAAc                           <  1:1523024/100‑1 (MQ=255)                                                                   tAGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAAc                           >  1:1750552/1‑100 (MQ=255)                                                                    aGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACa                          <  2:268566/100‑1 (MQ=255)                                                                    aGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACa                          <  2:760928/100‑1 (MQ=255)                                                                    aGGCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACa                          <  1:1078788/100‑1 (MQ=255)                                                                     ggCTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACAt                         >  1:2219544/1‑100 (MQ=255)                                                                       cTGTCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACAttg                       <  1:1681970/100‑1 (MQ=255)                                                                          tCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACAttgttg                    <  1:1353146/100‑1 (MQ=255)                                                                          tCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACAttgttg                    <  1:1674579/100‑1 (MQ=255)                                                                          tCGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACAttgttg                    <  2:244049/100‑1 (MQ=255)                                                                           cGGGAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGa                   >  1:2424687/1‑100 (MQ=255)                                                                            gggAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAg                  >  1:2330007/1‑100 (MQ=255)                                                                            gggAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAg                  >  2:1806069/1‑100 (MQ=255)                                                                             ggAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTTAGc                 <  2:2384857/100‑1 (MQ=255)                                                                              gAAACGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCg                <  2:679188/100‑1 (MQ=255)                                                                              gAAACGGGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCGGGATACCCACGCGCTACAGCGTGGCGGGCGAACATTGTTGAGCg                <  2:1673421/100‑1 (MQ=255)                                                                                 aCGTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACa             <  1:1239637/100‑1 (MQ=255)                                                                                   gTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACaaa           >  1:10469/1‑100 (MQ=255)                                                                                   gTGCGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACaaa           >  1:2010500/1‑100 (MQ=255)                                                                                     gCGAGCTACCGCGGATCGAACTCGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGGGCCGGGCGAACATTGTTGAGCGACAAAcc         <  2:466879/100‑1 (MQ=255)                                                                                      cGAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAAccc        >  2:620224/1‑100 (MQ=255)                                                                                       gAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCg       >  1:686494/1‑100 (MQ=255)                                                                                       gAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCg       >  1:315474/1‑100 (MQ=255)                                                                                       gAGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCg       >  1:2321567/1‑100 (MQ=255)                                                                                        aGCTACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGt      <  1:2218287/100‑1 (MQ=255)                                                                                        aGCGACGGCGGATCGAACTGGTAAAAATGATGGTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGTGAACATTGTTGAGCGACCAACCCGt      >  2:1048482/1‑100 (MQ=255)                                                                                           tACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGc   >  2:1922182/1‑100 (MQ=255)                                                                                           tACCGCGGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGc   <  1:1302239/100‑1 (MQ=255)                                                                                            aCCGCGGATCGAACTGGTAACAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGcc  >  1:2067063/1‑100 (MQ=255)                                                                                                 |                                                                                               CTCACTTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTGGGGCGCGAGCTGGATACCCACGCGCTACAGCGTGCCGGGCGAACATTGTTGAGCGACAAACCCGTTGCC  >  NC_000913/4451662‑4451852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑