Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	275,534	G→A	S70S (TCG→TCA)	mmuP →	CP4‑6 prophage; putative S‑methylmethionine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	275,534	0	G	A	100.0%	316.1 / NA	92	S70S (TCG→TCA)	mmuP	CP4‑6 prophage; putative S‑methylmethionine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/47);  total (45/47)

CATCATTTCCACCACTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACC  >  NC_000913/275438‑275630                                                                                                 |                                                                                                 catcatTTCCACCACTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTgtcagtc                                                                                               >  1:1772264/1‑100 (MQ=255)  atcatTTCCACCACTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCAGGCTGGTTATCCCGAGTCTGGGCGAGCTgtcagtcg                                                                                              >  2:2459665/1‑100 (MQ=255)   tcatTTCCACCACTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTcgc                                                                                             <  1:1186078/100‑1 (MQ=255)   tcatTTCCACCACTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTcgc                                                                                             <  1:742516/100‑1 (MQ=255)   tcatTTCCACCACAGGAGCGGCGGGAACGCTGCTGGGCTATCTGAGTTGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTcgc                                                                                             <  2:387108/100‑1 (MQ=255)    catTTCCACCACTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGGTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTcgcg                                                                                            >  2:1764062/1‑100 (MQ=255)         ccaccaCTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGcn                                                                                       <  2:2182829/100‑2 (MQ=255)         ccaccaCTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGcc                                                                                       <  1:766981/100‑1 (MQ=255)          caccacTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCg                                                                                      >  2:306919/1‑100 (MQ=255)           accacTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCgg                                                                                     <  2:2231692/100‑1 (MQ=255)           accacTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCgg                                                                                     >  1:347231/1‑100 (MQ=255)            ccacTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGga                                                                                    <  1:12254/100‑1 (MQ=255)            ccacTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGga                                                                                    <  2:1981853/100‑1 (MQ=255)               cTGGAGCGGCGGGAACGCTGCTGGCGTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGGGCTGTCAGTCGCGATGCCGGAGAc                                                                                 <  2:7885/100‑1 (MQ=255)               cTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGAc                                                                                 <  1:676142/100‑1 (MQ=255)               cTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGAc                                                                                 <  1:676288/100‑1 (MQ=255)                tGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTCTGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGAcc                                                                                >  2:406169/1‑100 (MQ=255)                tGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGAcc                                                                                <  2:1505533/100‑1 (MQ=255)                 ggAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCg                                                                               >  2:1759254/1‑100 (MQ=255)                  gAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCgg                                                                              <  1:1831033/100‑1 (MQ=255)                    gcggcgGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGCCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAg                                                                            >  2:244775/1‑100 (MQ=255)                     cggcggGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGc                                                                           <  1:623238/100‑1 (MQ=255)                     cggcggGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGc                                                                           <  1:622970/100‑1 (MQ=255)                      ggcggGAACGCTGCTGGCCTATCTGATGCGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCg                                                                          >  1:2324422/1‑100 (MQ=255)                        cggGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGtt                                                                        <  1:1186190/100‑1 (MQ=255)                        cggGAACGCTGCTGGCCTATCGGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGtt                                                                        <  2:857798/100‑1 (MQ=255)                          ggAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTc                                                                      <  1:1536669/100‑1 (MQ=255)                            aaCGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCAc                                                                    <  2:1947422/100‑1 (MQ=255)                              cGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGt                                                                  <  2:1745381/100‑1 (MQ=255)                              cGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGt                                                                  <  2:1911910/100‑1 (MQ=255)                               gctgctGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGtt                                                                 <  2:2359001/100‑1 (MQ=255)                               gctgctGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGtt                                                                 <  2:1095624/100‑1 (MQ=255)                                 tgctgGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTa                                                               <  1:2150689/100‑1 (MQ=255)                                  gctgGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTAt                                                              <  2:1704017/100‑1 (MQ=255)                                   ctgGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATg                                                             <  1:1895206/100‑1 (MQ=255)                                    tgGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGc                                                            <  1:661000/100‑1 (MQ=255)                                    tgGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGc                                                            <  1:477782/100‑1 (MQ=255)                                     ggCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGcc                                                           >  1:236908/1‑100 (MQ=255)                                     ggCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGcc                                                           >  2:1076639/1‑100 (MQ=255)                                     cgCCTATCTGCTTGGTGCGCTGGTGGTCTGGCTGGTTAGGCAGTGTCTGGGCGTGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGGTTATGcc                                                           >  2:1772416/2‑100 (MQ=255)                                       ccTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGAGGCCGGAGACCGGAGCGTTTCACGTTTATGCcgc                                                         >  2:369615/1‑100 (MQ=255)                                        cTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCcgcg                                                        <  2:1818648/100‑1 (MQ=255)                                          aTCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCcgcgcg                                                      <  2:2055007/100‑1 (MQ=255)                                          aTCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCcgcgcg                                                      <  2:793626/100‑1 (MQ=255)                                           tCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCgcgcgc                                                     >  2:1110238/1‑100 (MQ=255)                                            cTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCt                                                    >  2:127841/1‑100 (MQ=255)                                             tGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTa                                                   >  1:157163/1‑100 (MQ=255)                                             tGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTa                                                   >  1:1264529/1‑100 (MQ=255)                                             tGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGGGTCTGAGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCTGCGCGCTa                                                   <  2:1427487/100‑1 (MQ=255)                                               aTTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGCGCGTTTCACGTTTATGCCGCGCGCTATc                                                 >  2:1209709/1‑100 (MQ=255)                                                ttGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCt                                                >  2:1077496/1‑100 (MQ=255)                                                  ggTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTg                                              >  2:1173571/1‑100 (MQ=255)                                                  ggTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTg                                              >  2:1195492/1‑100 (MQ=255)                                                   gTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTgg                                             >  2:856662/1‑100 (MQ=255)                                                   gTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTgg                                             >  1:1289118/1‑100 (MQ=255)                                                    tGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGt                                            >  1:2116615/1‑100 (MQ=255)                                                     gcgcTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTc                                           >  1:1234748/1‑100 (MQ=255)                                                     gcgcTGGTGGTCTGGCTGGATATGCAGTGTCTGGGCGAGCTGTCAGCCGCGTTGCCGGAGACCGGAGCGTGTCGCGTTTATGCCGCGCGCTATCTTGGTc                                           >  2:2301431/1‑100 (MQ=255)                                                      cgcTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTcc                                          <  2:1031774/100‑1 (MQ=255)                                                       gcTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCg                                         >  2:2077899/1‑100 (MQ=255)                                                        cTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCgg                                        >  2:320282/1‑100 (MQ=255)                                                         tggtggTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGc                                       <  2:829912/100‑1 (MQ=255)                                                          ggtggtCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCt                                      >  1:719555/1‑100 (MQ=255)                                                           gtggtCTGGCTGGCTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTCTGCCGCGCGCTATCTTGGTCCGGCTa                                     >  2:2343478/1‑100 (MQ=255)                                                             ggtCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTAcc                                   <  2:1462017/100‑1 (MQ=255)                                                               tCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCgg                                 >  1:1892071/1‑100 (MQ=255)                                                                ctggctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTCCCggg                                <  2:2493447/100‑1 (MQ=255)                                                                ctggctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCggg                                <  2:1108533/100‑1 (MQ=255)                                                                ctggctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCggg                                <  2:1023518/100‑1 (MQ=255)                                                                ctggctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCggg                                >  1:520937/1‑100 (MQ=255)                                                                cgggctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGTCCGGAGCGTTTCACGTTTAGGCCGCGCGCTATCTTGGTCCGGCTACCggg                                <  2:2540639/98‑1 (MQ=255)                                                                 tggctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGt                               >  1:1339446/1‑100 (MQ=255)                                                                  ggctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGta                              >  2:1795169/1‑100 (MQ=255)                                                                  ggctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGta                              >  2:788420/1‑100 (MQ=255)                                                                   gctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGtat                             >  1:60726/1‑100 (MQ=255)                                                                    ctggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGGGGTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGtata                            >  2:590911/1‑100 (MQ=255)                                                                     tggTTATGGGGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGCGCGTTTCACGTTGATGCCGCGCGATATCTTGGTCCGGCTACCGGGTATAc                           <  2:479615/100‑1 (MQ=255)                                                                     tggTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATAc                           >  1:1908024/1‑100 (MQ=255)                                                                       gTTATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCg                         >  2:1695820/1‑100 (MQ=255)                                                                         tATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTg                       >  2:125387/1‑100 (MQ=255)                                                                         tATGCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTg                       >  2:413918/1‑100 (MQ=255)                                                                          aTGCGGTGTCTGGGCGAGCTGTCAGGCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTgg                      <  1:2407500/100‑1 (MQ=255)                                                                            gCAGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTGTCACGTTTATGCCGCGAGCTATCTTGGTCCGGCTACCGGGTATACCGTGGcc                    >  2:2447392/1‑100 (MQ=255)                                                                              aGTGTCTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTg                  >  2:2128745/1‑100 (MQ=255)                                                                                   cTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCttt             >  1:988979/1‑100 (MQ=255)                                                                                   cTGGGCGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCttt             <  2:425203/100‑1 (MQ=255)                                                                                        cGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTgg        <  1:1094252/100‑1 (MQ=255)                                                                                        cGAGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTgg        >  1:149509/1‑100 (MQ=255)                                                                                         gAGCTGTCAGTCGCGATGCCGGAGACCGGCGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGc       <  2:2226634/100‑1 (MQ=255)                                                                                          aGCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCt      <  1:1191944/100‑1 (MQ=255)                                                                                           gCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTg     <  2:1687868/100‑1 (MQ=255)                                                                                           gCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTg     <  2:1743281/100‑1 (MQ=255)                                                                                           gCTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTg     >  1:2389678/1‑100 (MQ=255)                                                                                            cTGTCAGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGa    >  1:456209/1‑100 (MQ=255)                                                                                              gtcagtcgCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGAcc  >  2:2173524/1‑100 (MQ=255)                                                                                                 |                                                                                                 CATCATTTCCACCACTGGAGCGGCGGGAACGCTGCTGGCCTATCTGATTGGTGCGCTGGTGGTCTGGCTGGTTATGCAGTGTCTGGGCGAGCTGTCGGTCGCGATGCCGGAGACCGGAGCGTTTCACGTTTATGCCGCGCGCTATCTTGGTCCGGCTACCGGGTATACCGTGGCCTGGCTTTACTGGCTGACC  >  NC_000913/275438‑275630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑