Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,809,774 (T)7→6 coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7680T.93.6% 713.1 / 39.4 171coding (152/738 nt)ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base T (7/4);  new base . (88/72);  total (95/76)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

GATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACC  >  NC_000913/2809669‑2809862
                                                                                                   |                                                                                              
gATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGt                                                                                                >  2:4837519/1‑100 (MQ=255)
 aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGtt                                                                                               <  2:192403/100‑1 (MQ=255)
  tGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttt                                                                                              <  1:4362002/100‑1 (MQ=255)
    cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttt                                                                                            >  1:5122927/1‑100 (MQ=255)
     aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGtttttt                                                                                           >  1:4527897/1‑100 (MQ=255)
     aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGtttttt                                                                                           <  1:1780657/100‑1 (MQ=255)
        gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttctc                                                                                        <  1:1178740/100‑4 (MQ=255)
        gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttctc                                                                                        >  2:2026079/1‑97 (MQ=255)
          cAGTTTACCGATTGATATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCt                                                                                     >  2:3214868/1‑100 (MQ=255)
            gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGc                                                                                   <  1:2051435/100‑1 (MQ=255)
            gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGc                                                                                   >  2:4654499/1‑100 (MQ=255)
            gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACACGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGc                                                                                   >  2:2918548/1‑100 (MQ=255)
              ttACCGATTGTTATTAGTTATATTCCGGTAGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGcat                                                                                 >  2:1145289/1‑100 (MQ=255)
                 ccGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGcatcat                                                                              <  2:5013241/100‑1 (MQ=255)
                   gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCAttt                                                                            >  1:4386790/1‑100 (MQ=255)
                   gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCAttt                                                                            >  2:1474863/1‑100 (MQ=255)
                    aTTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTa                                                                           >  2:3141959/1‑100 (MQ=255)
                      tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATg                                                                         >  2:3569532/1‑100 (MQ=255)
                      tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATg                                                                         <  2:3587038/100‑1 (MQ=255)
                      tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATg                                                                         <  1:2889027/100‑1 (MQ=255)
                       gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGc                                                                        >  2:4014948/1‑100 (MQ=255)
                       gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGc                                                                        >  2:2622804/1‑100 (MQ=255)
                       cttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGc                                                                        <  2:4602386/99‑1 (MQ=255)
                        ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCa                                                                       >  1:3140895/1‑100 (MQ=255)
                        ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCa                                                                       >  1:2339008/1‑100 (MQ=255)
                         tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCTTCTTTTATGCAg                                                                      >  2:2321870/1‑100 (MQ=255)
                         tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAg                                                                      >  1:1830345/1‑100 (MQ=255)
                          attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGTTTCTTTTATGCAgg                                                                     >  2:1308573/1‑100 (MQ=255)
                          attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCaag                                                                     >  1:2728459/1‑98 (MQ=255)
                           ttaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGgc                                                                    >  2:1119219/1‑100 (MQ=255)
                           ttaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATAAATGATGCAgga                                                                    <  2:3749279/100‑2 (MQ=255)
                                tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAg                                                               >  2:3148616/1‑100 (MQ=255)
                                 atatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGGTTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCCTCATTTATGCAGGCGCGAGc                                                              >  1:5154759/1‑100 (MQ=255)
                                  tcttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                             <  1:794088/98‑1 (MQ=255)
                                  tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                             <  1:678595/100‑1 (MQ=255)
                                  tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                             <  1:3127183/100‑1 (MQ=255)
                                  tatTCCGATGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGCATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                             <  1:4729123/100‑1 (MQ=255)
                                   atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCCACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTTCTTCATTTATGCATGCGCGAGCCa                                                            >  2:4572027/1‑100 (MQ=255)
                                    ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                           >  1:4218588/1‑100 (MQ=255)
                                      ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTTCTTTATCTTTTATGCAGGCGCGAGCCAGtt                                                         >  2:3205202/1‑100 (MQ=255)
                                      ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGtt                                                         <  2:1884248/100‑1 (MQ=255)
                                       cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                        <  2:1685041/100‑1 (MQ=255)
                                       cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                        >  2:3652210/1‑100 (MQ=255)
                                       cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                        <  1:3177486/100‑1 (MQ=255)
                                        ggTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                       >  1:1680721/1‑100 (MQ=255)
                                        ggTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                       >  1:2843863/1‑100 (MQ=255)
                                        ggTGGCATTTGCGTTCGGTATGACTGCGACCCGTCTGGGATCCTCTCCCCGCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGGGCCAGTTCg                                                       >  2:2421677/1‑100 (MQ=255)
                                         gTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTGATGCAGGCGCGAGCCAGTTCGt                                                      <  1:4312475/100‑1 (MQ=255)
                                           ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                                    <  2:1658044/100‑1 (MQ=255)
                                             ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                                  >  1:4956023/1‑100 (MQ=255)
                                             ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                                  <  2:446020/100‑1 (MQ=255)
                                             ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                                  >  2:1324471/1‑100 (MQ=255)
                                             ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                                  >  2:3761852/1‑100 (MQ=255)
                                               tttGCGTTCGGTCTGAATGTGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAc                                                >  2:2964318/1‑100 (MQ=255)
                                               tttGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTTTTTCTTCATTTATGCAGGCGCGAGCCAGTTCGTCATTAc                                                >  2:1378482/1‑100 (MQ=255)
                                               tttGCGTTCGGTCTGAACGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTCTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTCCGTCATTAc                                                <  2:1234155/100‑1 (MQ=255)
                                                 tGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                              >  2:2268358/1‑100 (MQ=255)
                                                  gCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                             <  1:1889777/100‑1 (MQ=255)
                                                   tgTTCGGTCTGACTGCGACCCTTCTGGGACTCTCTCCTCTCGAAAGCGGTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCCTTACcgca                                            <  2:2733893/99‑2 (MQ=255)
                                                           cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                    <  2:545148/100‑1 (MQ=255)
                                                           cTGAATGCGACCCGTCTGGGATTCCCCCCTCTCGAAAGCGTTTTTTCTACTGCTTCATTTATGCGGGCGCGAGCCAGTCCGTCATTACCGCGATGCTGGc                                    <  2:2787424/100‑1 (MQ=255)
                                                            tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGGCATTACCGCGATGCTGGCa                                   >  2:2298444/1‑100 (MQ=255)
                                                             gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                                  >  1:4069123/1‑100 (MQ=255)
                                                               aTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGcc                                >  2:33728/1‑100 (MQ=255)
                                                                 gCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                              <  1:4989205/100‑1 (MQ=255)
                                                                   gACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGa                            >  1:4157975/1‑100 (MQ=255)
                                                                   gACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGa                            >  2:1009236/1‑100 (MQ=255)
                                                                   gACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGa                            <  1:1310801/100‑1 (MQ=255)
                                                                     cccGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagt                          >  2:3267009/1‑100 (MQ=255)
                                                                     cccGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagt                          >  2:4432330/1‑100 (MQ=255)
                                                                      ccGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagta                         <  1:3468636/100‑1 (MQ=255)
                                                                         tCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt                      <  1:3814298/100‑1 (MQ=255)
                                                                           tGGGATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtg                    <  2:1300779/100‑1 (MQ=255)
                                                                            gggATTCTCTCCTCTCGAAAGCGTTTTTTTTTTTTCTTTATTGCTGCAAGCGCAGGCCATATCGTCATTACAGCGCTGCTGGCAGCGGGGggtagcccgt                   >  2:1622422/1‑95 (MQ=255)
                                                                            gggATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt                   <  1:2951612/100‑1 (MQ=255)
                                                                            gggATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt                   >  1:3243386/1‑100 (MQ=255)
                                                                             ggATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                  <  1:2406596/100‑1 (MQ=255)
                                                                              gATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                 >  1:4803356/1‑100 (MQ=255)
                                                                              gATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                 >  1:1133343/1‑100 (MQ=255)
                                                                              gATTCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                 <  1:599640/100‑1 (MQ=255)
                                                                                ttCTCTCCTCTCGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAt               >  1:3556703/1‑100 (MQ=255)
                                                                                  ctctcctctcGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAtt              >  2:831126/1‑100 (MQ=255)
                                                                                   tctcctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc            <  1:1629160/100‑1 (MQ=255)
                                                                                   tctcctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc            <  1:2316574/100‑1 (MQ=255)
                                                                                   tcgcctctcGAACGCGTTTTTTCTCCTGCATCAGTTATGCAGGCGCGGGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc            <  2:4633707/97‑1 (MQ=255)
                                                                                    ctcctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgct           >  1:3297652/1‑100 (MQ=255)
                                                                                     tcctctgGAAAGCGGTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg          <  2:4518463/100‑1 (MQ=255)
                                                                                     tcctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg          >  2:216327/1‑100 (MQ=255)
                                                                                     tcctctcGAAAGCGTTTTTTCGCGTGCATCATGTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg          <  2:1507609/100‑1 (MQ=255)
                                                                                      cctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc         >  1:1548880/1‑100 (MQ=255)
                                                                                      cctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc         >  2:1401060/1‑100 (MQ=255)
                                                                                      cctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc         <  1:370039/100‑1 (MQ=255)
                                                                                      cctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgcaga         >  2:2289096/1‑97 (MQ=255)
                                                                                       ctctcGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc         <  1:844411/100‑1 (MQ=255)
                                                                                       ctctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCa        <  1:2484799/100‑1 (MQ=255)
                                                                                        tctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc       <  2:3106080/100‑1 (MQ=255)
                                                                                        tctcGAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc       >  2:1835043/1‑100 (MQ=255)
                                                                                        atctgAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc       <  1:3530729/96‑1 (MQ=255)
                                                                                            gAAAGCGTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAcagat   >  2:3491952/1‑96 (MQ=255)
                                                                                             aaaGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAc   <  1:1960000/100‑1 (MQ=255)
                                                                                                   |                                                                                              
GATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGACC  >  NC_000913/2809669‑2809862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: