Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,397,649 Δ1 bp coding (238/1848 nt) mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,397,6460A.99.5% 919.9 / ‑2.5 192coding (235/1848 nt)mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (97/94);  total (98/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 201 total aligned reads displayed.

ATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTC  >  NC_000913/4397547‑4397741
                                                                                                   |                                                                                               
atCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAGCGGAAAAAAAAAAAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTa                                                                                                 <  2:1137577/100‑1 (MQ=255)
atCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTa                                                                                                 <  1:2891682/100‑1 (MQ=255)
  cGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa                                                                                               >  1:3405573/1‑100 (MQ=255)
    aaCGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaatc                                                                                             <  1:2306927/100‑3 (MQ=255)
       gcgGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGcc                                                                                         >  2:2704798/1‑100 (MQ=255)
        cgGTGGGGCGAGACGTATCCGCATTCGTGATAACGGCTGCGGTAACAAGAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCct                                                                                        >  1:4529007/1‑100 (MQ=255)
          gTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCctct                                                                                      >  2:814193/1‑100 (MQ=255)
           tGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCctctc                                                                                     >  1:3823888/1‑100 (MQ=255)
            ggggCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAGGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCctctct                                                                                    >  2:2392680/1‑100 (MQ=255)
            ggggCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCctctct                                                                                    <  2:1239570/100‑1 (MQ=255)
            ggggCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCctctct                                                                                    <  1:1000834/100‑1 (MQ=255)
              ggCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTgg                                                                                  >  2:3145858/1‑100 (MQ=255)
              ggCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTgg                                                                                  >  1:2034129/1‑100 (MQ=255)
              ggCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTgg                                                                                  >  1:2250856/1‑100 (MQ=255)
               gCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGa                                                                                 <  2:3385810/100‑1 (MQ=255)
                cGAAACTTATCCGCATTCGTGATAACGGCTGTTGTATCAAAAAAGATGAGCTGGCCCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTTGAc                                                                                <  2:4790006/100‑1 (MQ=255)
                cGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTgggc                                                                                >  1:1128630/1‑98 (MQ=255)
                cGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGAc                                                                                <  1:4861217/100‑1 (MQ=255)
                cGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGAc                                                                                >  1:2994165/1‑100 (MQ=255)
                 gAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACg                                                                               <  2:2965089/100‑1 (MQ=255)
                     cTTATCCGCATTCGTGATAACGTCTGCGGTATCAAAAAAGATGATCTGTCGCTGGCGCTGGCTCGTCATGCTCCCAGTAAATCGCCTCCCTGGCCGCtcg                                                                           >  2:4612567/1‑99 (MQ=255)
                     cTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGAtct                                                                           >  1:1620110/1‑100 (MQ=255)
                      ttATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTCGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGAtctc                                                                          >  2:98027/1‑100 (MQ=255)
                          ccGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAg                                                                      <  1:3741843/100‑1 (MQ=255)
                           cGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGc                                                                     >  2:4572084/1‑100 (MQ=255)
                           cGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGc                                                                     >  1:332799/1‑100 (MQ=255)
                           cGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGc                                                                     <  2:4919127/100‑1 (MQ=255)
                             cATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGTGCTGGCTCGTCAAGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCa                                                                   <  1:5017649/100‑1 (MQ=255)
                             cATTCGTGATAACGGCAGAGGAAAAAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCa                                                                   <  2:1309472/100‑1 (MQ=255)
                              aTTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTNGCGCTGGCGCTGGCTCNTCATGCCACCAGTAAATCGCCTCTCTGGCCNATCNCGAAGCCAt                                                                  >  1:3392335/1‑100 (MQ=255)
                                 cGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTAt                                                               <  1:31074/100‑1 (MQ=255)
                                 cGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTAt                                                               >  1:2184131/1‑100 (MQ=255)
                                  gTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATc                                                              <  2:2234872/100‑1 (MQ=255)
                                  gTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATc                                                              <  1:3655281/100‑1 (MQ=255)
                                   tGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCa                                                             <  1:3989986/100‑1 (MQ=255)
                                   tGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCa                                                             >  1:226705/1‑100 (MQ=255)
                                    gATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAg                                                            >  1:1586162/1‑100 (MQ=255)
                                     aTAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGc                                                           >  1:4137970/1‑100 (MQ=255)
                                        aCGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTg                                                        <  1:3146612/100‑1 (MQ=255)
                                         cGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTgg                                                       >  2:711589/1‑100 (MQ=255)
                                          ggCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTggg                                                      >  2:4647985/1‑100 (MQ=255)
                                           gCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGc                                                     >  1:1498648/1‑100 (MQ=255)
                                                 gTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTcgc                                               >  2:2113/1‑100 (MQ=255)
                                                 gTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTAGACGATCTCGAAGCCATTATCAGCCTGGGCTTTcgc                                               >  1:4851824/1‑100 (MQ=255)
                                                  tATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTcgcg                                              >  1:4293208/1‑100 (MQ=255)
                                                  tATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTcgcg                                              <  2:3858486/100‑1 (MQ=255)
                                                   aTCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGGCTGGGCTTTCGCgg                                             >  2:4987106/1‑100 (MQ=255)
                                                   aTCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCgg                                             <  1:1370092/100‑1 (MQ=255)
                                                   aTCAAAAAAGATGAGCTGGCGCTGGCGCTGGCGCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCgg                                             <  1:2460368/100‑1 (MQ=255)
                                                    tCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGt                                            >  1:591228/1‑100 (MQ=255)
                                                    tCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGt                                            <  2:1176800/100‑1 (MQ=255)
                                                      aaaaaaGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGa                                          >  2:1637444/1‑100 (MQ=255)
                                                      aaaaaaGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGa                                          >  1:3699925/1‑100 (MQ=255)
                                                      aaaaaaGATGAGCTGGAGCTGACGCTGGCGCGTCATGCCACCAGTAAATCGCCCCCCTTGACGATCTCGAAGCCATGATCAGCCTTGGGTTTCGCGGTGa                                          >  2:4488322/1‑100 (MQ=255)
                                                       aaaaaGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAg                                         <  2:4102167/100‑1 (MQ=255)
                                                       aaaaaGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAg                                         <  1:940979/100‑1 (MQ=255)
                                                          aaGATGAGCTGGCGCTGGCGCTGGCGCGTCATGCCACCAGTAAATCGCCTCGCTGGACGATCTCGAAGCCATTATCGGCCTGGGCTGTCGCGGTGAGgcg                                      >  2:3960760/1‑100 (MQ=255)
                                                           aGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGgcgc                                     <  1:3064098/100‑1 (MQ=255)
                                                            gATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCt                                    <  2:3717738/100‑1 (MQ=255)
                                                             aTGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTg                                   >  1:1534476/1‑100 (MQ=255)
                                                              tGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTgg                                  >  1:4635090/1‑100 (MQ=255)
                                                               gAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGc                                 <  1:1632265/100‑1 (MQ=255)
                                                                aGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCg                                <  1:767980/100‑1 (MQ=255)
                                                                aGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCg                                <  1:1716993/100‑1 (MQ=255)
                                                                    ggcgctggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTa                            >  2:2537166/1‑100 (MQ=255)
                                                                    ggcgctggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTa                            <  1:1285355/100‑1 (MQ=255)
                                                                     gcgttggcgctggtTGGTCATGCAACGAGTAAAGCGGCTGTCTGGAGGATAGCGAAGCCATTACTAGCCTGAGCTTTCGAGGTGGGGCGCTGGCGAGTAt                           <  2:4817455/100‑1 (MQ=255)
                                                                     gcgctggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTAt                           >  2:394298/1‑100 (MQ=255)
                                                                      cgctggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATc                          <  1:3181435/100‑1 (MQ=255)
                                                                      cgctggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATc                          <  2:3286864/100‑1 (MQ=255)
                                                                        ctggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAg                        <  1:3223194/100‑1 (MQ=255)
                                                                         tggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGt                       >  2:2315613/1‑100 (MQ=255)
                                                                         tggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGt                       >  1:2811689/1‑100 (MQ=255)
                                                                          ggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGACGCCATTATCAGCCTGGCCTTTCGCGGTGAGGCGCTGGCGAGTATCAGtt                      >  2:2667983/1‑100 (MQ=255)
                                                                          ggcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGtt                      <  2:4455080/100‑1 (MQ=255)
                                                                           gcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTc                     >  1:3597111/1‑100 (MQ=255)
                                                                           gcgctggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTc                     <  1:5099986/100‑1 (MQ=255)
                                                                             gatggcGCGTCATGCCCCAAGTACATCTCCTCCCTGGACGGTCTCGTAGCCATTATCATCCTGGGCTTTCGCGGTGAGGCGCTAGCGAGTATCAGTTCgg                   <  2:3597160/98‑1 (MQ=255)
                                                                              ctggcGCGTCACGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGt                  <  2:588687/100‑1 (MQ=255)
                                                                               tggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCGGGTCgggt                 >  2:3060085/1‑98 (MQ=255)
                                                                               tggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGtt                 <  1:869592/100‑1 (MQ=255)
                                                                                ggcTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGttt                <  2:2780205/100‑1 (MQ=255)
                                                                                 tcTCGTCCTGCCACCAGTAAGTGGGCTCTCTGGACGAGCTCGAAGCTATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTc               <  2:1150273/99‑1 (MQ=255)
                                                                                  cTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTcc              <  2:4758784/100‑1 (MQ=255)
                                                                                  cTCGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTcc              >  1:421774/1‑100 (MQ=255)
                                                                                    cGTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCg            >  1:2090083/1‑100 (MQ=255)
                                                                                     gTCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGc           <  2:1827324/100‑1 (MQ=255)
                                                                                      tCATGCCACCAGTAAATCGCCTCTCTGGACGCGCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGcc          <  2:1697934/100‑1 (MQ=255)
                                                                                      tCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGcc          <  1:1659986/100‑1 (MQ=255)
                                                                                      tCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGcc          >  1:4415840/1‑100 (MQ=255)
                                                                                      tCATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGcc          <  1:1967451/100‑1 (MQ=255)
                                                                                       cATGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCt         >  1:1828400/1‑100 (MQ=255)
                                                                                        aTGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTg        <  1:2343365/100‑1 (MQ=255)
                                                                                        aTGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTg        >  1:3239585/1‑100 (MQ=255)
                                                                                         tGCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATGATCTTCCTGGGCTATCGCGGCGAGTCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGa       >  2:2055358/1‑100 (MQ=255)
                                                                                          gCCACCAGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGACGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGAc      <  2:4946181/100‑1 (MQ=255)
                                                                                           ccaccgGTAAATCGCCTCTCTGGACGGTCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACg     <  2:2810713/100‑1 (MQ=255)
                                                                                           ccaccaGTAAATCGCCTCTCTGGACGATCGCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACg     <  2:1451070/100‑1 (MQ=255)
                                                                                              ccaGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTc  <  1:4555717/100‑1 (MQ=255)
                                                                                              ccaGTAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTc  <  2:1804710/100‑1 (MQ=255)
                                                                                                   |                                                                                               
ATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTC  >  NC_000913/4397547‑4397741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: