Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 345,973 (G)5→4 coding (365/672 nt) yahN ← amino acid exporter for proline, lysine, glutamate, homoserine

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913345,9690G.98.8% 786.6 / 4.7 169coding (369/672 nt)yahNamino acid exporter for proline, lysine, glutamate, homoserine
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base . (72/93);  total (73/96)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

Only 100 of 178 total aligned reads displayed.

TTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCGCCGCCGAC  >  NC_000913/345870‑346064
                                                                                                   |                                                                                               
ttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAg                                                                                                 <  1:1938874/100‑1 (MQ=255)
   ggCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAgggg                                                                                              >  1:1794164/1‑100 (MQ=255)
      aTTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAggggcgc                                                                                           >  2:4366012/1‑97 (MQ=255)
       tttAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAggggcgct                                                                                          <  1:1411670/100‑5 (MQ=255)
         tAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTaa                                                                                       >  1:1995449/1‑100 (MQ=255)
          aaTGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAAt                                                                                      <  2:338249/100‑1 (MQ=255)
           aTGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATc                                                                                     <  1:2567644/100‑1 (MQ=255)
           aTGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATc                                                                                     <  1:5101688/100‑1 (MQ=255)
             gTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCgg                                                                                   <  1:3112789/100‑1 (MQ=255)
             gTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCAGAAGAAAAAAAAGACATACCAGGGGCGCTAATCgg                                                                                   <  2:2318295/100‑1 (MQ=255)
              ttACTAAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACAGACCAGGGGCGCTAATCGgt                                                                                  <  2:4647040/100‑1 (MQ=255)
                 cTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTTGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGgttgt                                                                               <  1:2160449/100‑1 (MQ=255)
                 cTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGgttgt                                                                               >  1:2964886/1‑100 (MQ=255)
                 cTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGgttgt                                                                               >  2:1679233/1‑100 (MQ=255)
                 cTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGgttgt                                                                               >  2:3138820/1‑100 (MQ=255)
                 cTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGgttgt                                                                               >  2:3334026/1‑100 (MQ=255)
                 cTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGgttgt                                                                               <  2:3797178/100‑1 (MQ=255)
                 cTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACAAGGGGCGCTAATCGgatgt                                                                               >  2:4421658/1‑100 (MQ=255)
                 cTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGATAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGgttgt                                                                               <  1:4927984/100‑1 (MQ=255)
                  tGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGgttgtt                                                                              <  2:1341694/100‑1 (MQ=255)
                   gagaAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGttgttg                                                                             >  1:1392655/1‑100 (MQ=255)
                   gagaAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGttgttg                                                                             >  2:676701/1‑100 (MQ=255)
                    agaAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTtgttgt                                                                            <  2:3576186/100‑1 (MQ=255)
                     gaAAATACTGGTAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATTCCAGGGGCGCTAGTCGGTTGTTGTa                                                                           <  2:3959847/100‑1 (MQ=255)
                     gaAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATTGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTa                                                                           >  1:1106945/1‑100 (MQ=255)
                      aaaaTACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAg                                                                          <  1:1500675/100‑1 (MQ=255)
                      aaaaTACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAg                                                                          <  1:4144271/100‑1 (MQ=255)
                       aaaTACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAgt                                                                         >  2:1940523/1‑100 (MQ=255)
                           aCTGATCAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGTGGCGCTAATCGGTTGTTGTAGTGTGc                                                                     <  2:1507083/100‑1 (MQ=255)
                           aCTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGc                                                                     <  2:423473/100‑1 (MQ=255)
                              gATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCa                                                                  >  2:1207291/1‑100 (MQ=255)
                              gATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCa                                                                  >  1:4991027/1‑100 (MQ=255)
                              gATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCa                                                                  >  2:949593/1‑100 (MQ=255)
                               aTAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTCATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAt                                                                 >  2:4106180/1‑100 (MQ=255)
                               aTAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAt                                                                 >  1:4219769/1‑100 (MQ=255)
                               aTAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAt                                                                 >  1:4441817/1‑100 (MQ=255)
                               aTAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAt                                                                 >  1:579695/1‑100 (MQ=255)
                                tAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAtt                                                                >  1:1220746/1‑100 (MQ=255)
                                tAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAtt                                                                <  1:303257/100‑1 (MQ=255)
                                tAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAtt                                                                <  1:531310/100‑1 (MQ=255)
                                tAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAtt                                                                >  2:2457981/1‑100 (MQ=255)
                                 aaaaaaTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAttt                                                               >  2:4668624/1‑100 (MQ=255)
                                 aaaaaaTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCAttt                                                               >  2:1132840/1‑100 (MQ=255)
                                  aaaaaTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTg                                                              >  2:2882074/1‑100 (MQ=255)
                                   aaaaTAAAGCGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACACACCAGGGGCGCTGATCGGTTGTTGTAGTGTGCTCATTTGc                                                             >  2:4861061/1‑100 (MQ=255)
                                   aaaaTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGc                                                             >  2:2788261/1‑100 (MQ=255)
                                    aaaTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCg                                                            >  1:1298823/1‑100 (MQ=255)
                                     aaTAGAGCGGTTTGCGGGTTAGAGGGATCGGTAATTATTGCGGGGGGAAGAAGGACTTACGAGGGGGGCTAATCGGTTGTTGTAGTGTGCTCATTTGCgg                                                           <  2:4094491/100‑1 (MQ=255)
                                     aaTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCgg                                                           >  2:530154/1‑100 (MQ=255)
                                     aaTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCgg                                                           <  1:1346892/100‑1 (MQ=255)
                                     aaTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCgg                                                           <  2:1050913/100‑1 (MQ=255)
                                      aTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTTCTCATTTGCGgt                                                          <  1:4437870/100‑1 (MQ=255)
                                      aTAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGgt                                                          >  1:154596/1‑100 (MQ=255)
                                       tAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGgtg                                                         <  2:600029/100‑1 (MQ=255)
                                        aaaaCGGTTTGTGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGgtgt                                                        >  1:3564999/1‑100 (MQ=255)
                                        aaaaCGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGgtgt                                                        <  1:3174313/100‑1 (MQ=255)
                                         aaaCGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGtt                                                       >  2:2959448/1‑100 (MQ=255)
                                         aaaCGGTTGGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGATCATTTGCGGTGtt                                                       >  2:1213906/1‑100 (MQ=255)
                                           aCGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGTGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGa                                                     <  1:4854062/100‑1 (MQ=255)
                                           aCGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGa                                                     >  1:2819662/1‑100 (MQ=255)
                                           aCGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGa                                                     <  2:1693680/100‑1 (MQ=255)
                                            cGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGAc                                                    <  1:4076925/100‑1 (MQ=255)
                                             ggTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACt                                                   <  2:4914481/100‑1 (MQ=255)
                                                 tGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTggcg                                               >  1:2338980/1‑100 (MQ=255)
                                                  gCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTggcgg                                              <  2:3917098/100‑1 (MQ=255)
                                                  gCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTggcgg                                              >  1:2480515/1‑100 (MQ=255)
                                                   cGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTggcggc                                             <  1:1918939/100‑1 (MQ=255)
                                                      gTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCa                                          <  2:1970493/100‑1 (MQ=255)
                                                        tAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCAtg                                        >  1:2502033/1‑100 (MQ=255)
                                                         agagagATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCAtgc                                       >  1:2757793/1‑100 (MQ=255)
                                                         agagagACCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCAtgc                                       <  2:4725300/100‑1 (MQ=255)
                                                          gagagaTCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCAtgct                                      <  1:787802/100‑1 (MQ=255)
                                                            gagaTCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCAtgctgc                                    <  2:2635476/100‑1 (MQ=255)
                                                             agaTCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGca                                   <  1:8129/100‑1 (MQ=255)
                                                             agaTCGGTAATAAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTGGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGca                                   <  2:3980047/100‑1 (MQ=255)
                                                               aTCGGTAGTTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGtacc                                 <  1:2094329/100‑1 (MQ=255)
                                                                tCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGcacca                                >  1:1094608/1‑100 (MQ=255)
                                                                tCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGcacca                                >  1:3165417/1‑100 (MQ=255)
                                                                 cGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGcacca                                <  1:4293465/100‑1 (MQ=255)
                                                                       ttaatCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAAc                         <  2:3698408/100‑1 (MQ=255)
                                                                       ttaatCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAAc                         >  2:4242048/1‑100 (MQ=255)
                                                                         aatCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCa                       >  2:2010622/1‑100 (MQ=255)
                                                                          atCCGCGGCTAAAAAAGACATACCAGGGGCGCTAATCGGTTGCTGTAGTGTGCTCATTTGGGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCAt                      <  1:4495289/100‑1 (MQ=255)
                                                                           tCCGCGGCGAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATa                     <  1:4193745/100‑1 (MQ=255)
                                                                            ccGCGGCGAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAag                   <  1:5017015/100‑1 (MQ=255)
                                                                               cggcgAAAAAAGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAagag                 <  1:964879/100‑1 (MQ=255)
                                                                                  cgAAAAAAGACATACCAGGAGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCACGCTGCACCACGCAAACCATAAGAGATa              >  2:1850203/1‑100 (MQ=255)
                                                                                    aaaaaaGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAg            >  2:2382188/1‑100 (MQ=255)
                                                                                     aaaaaGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAgc           <  1:4744501/100‑1 (MQ=255)
                                                                                        aaGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGcgcc        >  2:2874037/1‑100 (MQ=255)
                                                                                         aGACATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGcgccc       >  1:2217473/1‑99 (MQ=255)
                                                                                           aCATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCgccgcc     <  1:1758123/100‑1 (MQ=255)
                                                                                           aCATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCgccgcc     <  1:3274639/100‑1 (MQ=255)
                                                                                           aCATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCgccgcc     <  1:4223224/100‑1 (MQ=255)
                                                                                            cATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCGccgccg    <  1:3777242/100‑1 (MQ=255)
                                                                                            cATACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCGccgccg    <  1:3779655/100‑1 (MQ=255)
                                                                                              tACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCGCCGCcgac  >  1:2262218/1‑100 (MQ=255)
                                                                                              tACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCGCCGCcgac  <  2:2491840/100‑1 (MQ=255)
                                                                                              tACCAGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCGCCGCcgac  >  2:892953/1‑100 (MQ=255)
                                                                                              tACCAGGGGCGCTAAGCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCGCCGCcgac  <  2:4047628/100‑1 (MQ=255)
                                                                                                   |                                                                                               
TTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGGCGCTAATCGGTTGTTGTAGTGTGCTCATTTGCGGTGTTGACTGGCGGCGCATGCTGCACCACGCAAACCATAAGAGATAAGCGCCGCCGAC  >  NC_000913/345870‑346064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: