Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,875,056 (C)6→7 coding (521/822 nt) yeaM ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,875,0501.C94.0% 388.5 / 18.5 117coding (527/822 nt)yeaMputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (4/3);  new base C (49/61);  total (53/64)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

TGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCT  >  NC_000913/1874951‑1875143
                                                                                                    |                                                                                                
tgctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATg                                                                                                   <  2:1045381/100‑1 (MQ=255)
 nctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                 >  1:1567967/2‑100 (MQ=255)
 gctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                 <  1:4652513/100‑1 (MQ=255)
 gctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                 >  1:587030/1‑100 (MQ=255)
   tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccc                                                                                               >  1:1350272/1‑100 (MQ=255)
    gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccc                                                                                              >  1:2929789/1‑100 (MQ=255)
    gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccc                                                                                              <  1:2528553/100‑1 (MQ=255)
    gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccc                                                                                              >  2:3159771/1‑100 (MQ=255)
    gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccc                                                                                              <  2:1838702/100‑1 (MQ=255)
    gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccc                                                                                              <  2:1628202/100‑1 (MQ=255)
      cGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccc                                                                                          >  2:4772007/1‑100 (MQ=255)
      cGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGAGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccc                                                                                          <  2:3037070/100‑1 (MQ=255)
        ccACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccccca                                                                                        >  1:4931941/1‑98 (MQ=255)
           cTGGTGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAACCAGCCCATTGGCGCAATGcccccccacta                                                                                     >  2:4293258/1‑95 (MQ=255)
            tGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCg                                                                                    <  2:1988885/100‑1 (MQ=255)
               cGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACa                                                                                 >  2:4350383/1‑100 (MQ=255)
               cGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACa                                                                                 >  2:3531078/1‑100 (MQ=255)
                gAAAACTCAACCCCGTCTCTTTTACTATTAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCGCCACTCGACAg                                                                                >  1:3606424/1‑100 (MQ=255)
                gAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAg                                                                                <  2:3012426/100‑1 (MQ=255)
                  aaaCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGc                                                                              <  2:4644478/100‑1 (MQ=255)
                  aaaCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGc                                                                              >  2:1343169/1‑100 (MQ=255)
                   aaCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGcc                                                                             <  1:425727/100‑1 (MQ=255)
                     cTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCt                                                                           >  1:1303340/1‑100 (MQ=255)
                     cTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCAACAGGCCCt                                                                           >  1:2979701/1‑100 (MQ=255)
                      tCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCtt                                                                          >  1:1677809/1‑100 (MQ=255)
                       cAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCttt                                                                         >  1:3950610/1‑100 (MQ=255)
                        aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTc                                                                        <  2:4145855/100‑1 (MQ=255)
                         aCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCg                                                                       <  2:3624618/100‑1 (MQ=255)
                           ccccTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGcc                                                                     <  1:4235103/100‑1 (MQ=255)
                             cGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCcat                                                                   <  1:3292919/100‑1 (MQ=255)
                              gTCTCTTTTACTATCAGTCGGGCAAGATTGCGTAAAAAAAAAGAAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCcatc                                                                  <  2:4400523/100‑1 (MQ=255)
                               tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCcatca                                                                 >  1:1703798/1‑100 (MQ=255)
                                ccctTTTAATATCAGTCGCGCAAGCTTGAGTTAACAAAACACAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCcatcat                                                                <  2:4923763/98‑1 (MQ=255)
                                 tctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCatcatc                                                               <  1:3826858/100‑1 (MQ=255)
                                  cgtttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCTACTCGACAGGCCCTTTCGCCATCAtct                                                              <  2:5052825/98‑1 (MQ=255)
                                   ttttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCAtctc                                                             >  1:550168/1‑100 (MQ=255)
                                    tttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTcc                                                            <  2:566291/100‑1 (MQ=255)
                                    tttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTcc                                                            <  2:2897084/100‑1 (MQ=255)
                                     ttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTcca                                                           <  1:4211139/100‑1 (MQ=255)
                                     caaCTGTCAGACGGGCAAGCTTGCGTTCACTCATCGCAAAAACGCCAGCCCATTGCCACAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTcca                                                           <  2:3056900/98‑1 (MQ=255)
                                      tACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTccac                                                          <  2:2431468/100‑1 (MQ=255)
                                      tACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTccac                                                          >  1:867514/1‑100 (MQ=255)
                                      tACTATCAGTCGGGCAAGATTGCGTTCACACAACGAAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTccac                                                          <  2:2970720/100‑1 (MQ=255)
                                        cTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTccacca                                                        >  1:2395197/1‑100 (MQ=255)
                                         tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCCTTGCCCCACTGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCAt                                                       >  1:1560603/1‑100 (MQ=255)
                                         tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCAt                                                       <  2:2983865/100‑1 (MQ=255)
                                          aTCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATc                                                      >  1:247132/1‑100 (MQ=255)
                                           tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCg                                                     <  1:2996086/100‑1 (MQ=255)
                                              gTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                  <  1:45180/100‑1 (MQ=255)
                                               tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCg                                                 <  2:3230595/100‑1 (MQ=255)
                                                cGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCgg                                                <  1:3635623/100‑1 (MQ=255)
                                                 gggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGa                                               >  1:936937/1‑100 (MQ=255)
                                                  ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAt                                              >  2:288424/1‑100 (MQ=255)
                                                  ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAt                                              >  1:50571/1‑100 (MQ=255)
                                                    cAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAttt                                            >  2:2780178/1‑100 (MQ=255)
                                                     aaGATTGCGTTCACTCATCGCAAAAACGCCAGCCCATTGCCCCACTGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAtttt                                           >  2:2445664/1‑100 (MQ=255)
                                                      aGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTa                                          <  1:2519232/100‑1 (MQ=255)
                                                       gATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAg                                         >  1:4863743/1‑100 (MQ=255)
                                                       gATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAg                                         <  2:1915186/100‑1 (MQ=255)
                                                        aTTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg                                        >  2:3799403/1‑100 (MQ=255)
                                                        aTTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg                                        >  2:613663/1‑100 (MQ=255)
                                                         ttGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGa                                       <  1:2276564/100‑1 (MQ=255)
                                                           gCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATg                                     <  2:1394323/100‑1 (MQ=255)
                                                            cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATga                                    >  1:3110854/1‑100 (MQ=255)
                                                            cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATga                                    <  2:1570124/100‑1 (MQ=255)
                                                            cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATga                                    >  1:711957/1‑100 (MQ=255)
                                                              ttCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATgaga                                  <  1:2145067/100‑1 (MQ=255)
                                                              ttCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATgaga                                  <  1:2144834/100‑1 (MQ=255)
                                                                 aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCTCCGTGCGGAGTTTAGGATGagcaga                               >  2:4074200/1‑100 (MQ=255)
                                                                      tCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACgg                          >  2:1892808/1‑100 (MQ=255)
                                                                      tCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACgg                          <  1:1964729/100‑1 (MQ=255)
                                                                       cGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACggg                         <  2:1680511/100‑1 (MQ=255)
                                                                       cGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGACATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACggg                         >  2:4756219/1‑100 (MQ=255)
                                                                        gCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc                        <  1:3840826/100‑1 (MQ=255)
                                                                        gCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc                        <  2:2194445/100‑1 (MQ=255)
                                                                        gCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc                        >  2:3971622/1‑100 (MQ=255)
                                                                        gCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCGCACTCGACAGGCCCTTTCGCCATCATCTCGACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc                        >  1:4859774/1‑100 (MQ=255)
                                                                         cAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCa                       >  1:1790007/1‑100 (MQ=255)
                                                                               agccagccCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGACATCATCTCCACCATCGTGCGGATTTTAGGATGGGAAGAAACGGGCAGGTGCa                 >  1:4586458/1‑100 (MQ=255)
                                                                                gccagccCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAg                >  1:1917844/1‑100 (MQ=255)
                                                                                 ccagccCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGc               <  1:4004420/100‑1 (MQ=255)
                                                                                   agccCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtg             <  1:443133/100‑1 (MQ=255)
                                                                                    gccCATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgt            <  2:3213322/100‑1 (MQ=255)
                                                                                     cccATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgtt           <  1:3570188/100‑1 (MQ=255)
                                                                                     cccATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgtt           >  2:2436871/1‑100 (MQ=255)
                                                                                       cATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgt         <  2:635404/100‑1 (MQ=255)
                                                                                       cATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgt         >  2:1337873/1‑100 (MQ=255)
                                                                                       cATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgt         <  2:2221260/100‑1 (MQ=255)
                                                                                       cATTGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgt         <  1:155896/100‑1 (MQ=255)
                                                                                        aTTGCCCCAATGCCCCCCCACTCGACAGACCCTTTCGCCATCATCTCCACCATCGGGCGGATTTTAGGATGAGAAGAAGCGGGGAGGTGCAGCtgtagtt        <  2:154007/100‑1 (MQ=255)
                                                                                         ttGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgttg       <  2:49866/100‑1 (MQ=255)
                                                                                          tGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgttgt      <  2:374676/100‑1 (MQ=255)
                                                                                          tGCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgttgt      >  2:5180/1‑100 (MQ=255)
                                                                                           gCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg     >  1:795739/1‑100 (MQ=255)
                                                                                           gCCCCAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg     <  1:4016139/100‑1 (MQ=255)
                                                                                           gCCCCAATGCCCCCGCACTCGACAGGCCCTTTCGCCATCCTCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg     <  1:3646494/100‑1 (MQ=255)
                                                                                             cccAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGgc   <  1:1025216/100‑1 (MQ=255)
                                                                                             cccAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGgc   <  1:3653321/100‑1 (MQ=255)
                                                                                              ccAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGgct  >  1:1788321/1‑100 (MQ=255)
                                                                                              ccAATGCCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAAATTAGGATAAAAAAAAACTAACAGGTGCAGCTGTTGTTGTGgct  >  1:570124/1‑100 (MQ=255)
                                                                                                    |                                                                                                
TGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCT  >  NC_000913/1874951‑1875143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: