Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,090,136 (G)5→6 intergenic (+90/‑56) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1311.G91.0% 577.3 / 53.4 177intergenic (+85/‑61)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base . (8/8);  new base G (81/80);  total (89/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

CATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGA  >  NC_000913/2090032‑2090228
                                                                                                     |                                                                                                  
 atcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCg                                                                                                   >  1:3557182/1‑100 (MQ=255)
  tcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                                  <  1:194093/100‑1 (MQ=255)
    atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                               <  1:1308093/100‑1 (MQ=255)
    atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                               >  1:1622225/1‑100 (MQ=255)
    atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                               >  2:3667941/1‑100 (MQ=255)
    atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCAAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                               >  2:1471443/1‑100 (MQ=255)
     tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggggg                                                                                              <  1:4259877/100‑1 (MQ=38)
     tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                                                                              <  2:4492012/100‑1 (MQ=255)
      ccTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCCGGAAGGTGATCTTCCggggg                                                                                              >  1:226825/1‑100 (MQ=255)
      ccTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggggg                                                                                             <  1:2149044/100‑2 (MQ=255)
       cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggggc                                                                                            <  2:938443/100‑3 (MQ=255)
        tGACTAGTCTTTCAGGCGATGTGTGCTGGTAGACATTCAGATCTTCCAGTGGTGCATGGACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggggct                                                                                           <  2:4834623/100‑4 (MQ=255)
        tGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCCGGAAGGTGATCTTCCGGGGGCt                                                                                            <  2:4391278/100‑1 (MQ=38)
        tGACTAGTCGTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCCGGAAGGTGATCTTCCGGGGGCt                                                                                            <  2:4063648/100‑1 (MQ=38)
             aGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCtttttt                                                                                       <  2:1019355/100‑1 (MQ=255)
             aGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCtttttt                                                                                       >  2:2466957/1‑100 (MQ=255)
                cTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTAtt                                                                                    <  2:2364556/100‑1 (MQ=255)
                cTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTAtt                                                                                    <  1:5085840/100‑1 (MQ=255)
                 tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTg                                                                                   >  1:2948362/1‑100 (MQ=255)
                 tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTg                                                                                   <  2:266996/100‑1 (MQ=255)
                    gaGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCCTGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCCTTTTTATTgcgg                                                                                >  2:2764050/2‑99 (MQ=255)
                    cacgCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTgcgc                                                                                >  1:1749709/4‑100 (MQ=255)
                    cAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTgcgc                                                                                >  2:3600371/1‑100 (MQ=255)
                      ggCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCgg                                                                              <  2:113209/100‑1 (MQ=255)
                       gCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGt                                                                             <  1:4671374/100‑1 (MQ=255)
                        cGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGtt                                                                            <  1:2696925/100‑1 (MQ=255)
                         gATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTg                                                                           <  1:4956288/100‑1 (MQ=255)
                         gATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTg                                                                           <  2:4659474/100‑1 (MQ=255)
                         gATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTg                                                                           <  1:1492726/100‑1 (MQ=255)
                           tgtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGAt                                                                         <  2:2191441/100‑1 (MQ=255)
                           tgtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGAt                                                                         <  1:474490/100‑1 (MQ=255)
                            gtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATa                                                                        >  2:1094379/1‑100 (MQ=255)
                            gtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATa                                                                        <  2:19883/100‑1 (MQ=255)
                               tgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACg                                                                     >  1:4388148/1‑100 (MQ=255)
                                 cTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGt                                                                   <  2:2797400/100‑1 (MQ=255)
                                   ggAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                                 <  2:1599937/100‑1 (MQ=255)
                                   ggAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                                 <  1:3640141/100‑1 (MQ=255)
                                   ggAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                                 <  1:201594/100‑1 (MQ=255)
                                     aaGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAg                                                               <  1:1838546/100‑1 (MQ=255)
                                         cATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAg                                                           >  2:2594508/1‑100 (MQ=255)
                                          aTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAgg                                                          <  2:65786/100‑1 (MQ=255)
                                          aTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAgg                                                          >  1:1593164/1‑100 (MQ=255)
                                            tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt                                                        >  1:3508483/1‑100 (MQ=255)
                                             cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt                                                       <  2:2147867/100‑1 (MQ=255)
                                             cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt                                                       >  1:4713503/1‑100 (MQ=255)
                                              aGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                      >  1:3308058/1‑100 (MQ=255)
                                              aGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                      <  2:3698726/100‑1 (MQ=255)
                                              aGATCGGCCAGTGGTGCATGAGCGCATGAGAAAGCCCCCGGAAGATGACCTTTCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                      <  2:4749882/100‑1 (MQ=255)
                                               gATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                     <  1:4988116/100‑1 (MQ=255)
                                                aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaaa                                                    >  1:1013960/1‑100 (MQ=255)
                                                aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaaa                                                    <  1:2182809/100‑1 (MQ=255)
                                                  cTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga                                                  <  2:4744746/100‑1 (MQ=255)
                                                   ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAagag                                                 >  2:2574723/1‑100 (MQ=255)
                                                    tCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAgg                                                <  2:778936/100‑1 (MQ=255)
                                                    tCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAgg                                                >  1:3757680/1‑100 (MQ=255)
                                                     ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGa                                               >  1:380598/1‑100 (MQ=255)
                                                      cAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                              >  2:3520439/1‑100 (MQ=255)
                                                      cAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                              >  2:1780552/1‑100 (MQ=255)
                                                      cAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGAGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                              <  1:848756/100‑1 (MQ=255)
                                                       aGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAAt                                             >  1:1447601/1‑100 (MQ=255)
                                                          ggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAAc                                          <  2:4264526/100‑1 (MQ=255)
                                                          ggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTGTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAAc                                          >  2:4599507/1‑100 (MQ=255)
                                                            tgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaa                                        <  2:119043/100‑1 (MQ=255)
                                                             gCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaaa                                       >  1:1208900/1‑100 (MQ=255)
                                                             gCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaaa                                       >  2:3117677/1‑100 (MQ=255)
                                                              cATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGCTAACGGTTCAGACAGGTTTAACGAGGAATAACaaaa                                      >  1:1781157/1‑100 (MQ=255)
                                                                tGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATg                                    <  1:2294530/100‑1 (MQ=255)
                                                                   actcATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGTTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca                                 <  1:5155642/97‑1 (MQ=255)
                                                                   aCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACCGTTCAGAAAGGTTTAAAGAGGAATAACAAAATgaca                                 >  2:3327791/1‑100 (MQ=255)
                                                                    cGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATggcag                                >  1:4706953/1‑100 (MQ=255)
                                                                    cGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacag                                <  2:243497/100‑1 (MQ=255)
                                                                     gCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacaga                               <  1:4495136/100‑1 (MQ=255)
                                                                      cATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                              <  2:3271391/100‑1 (MQ=255)
                                                                       aTGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagaca                             <  2:1427703/100‑1 (MQ=255)
                                                                        tGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaa                            >  2:2935908/1‑100 (MQ=255)
                                                                         gagaAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaac                           <  2:3440182/100‑1 (MQ=255)
                                                                         gagaAAGCCCCCGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaac                           >  2:325290/1‑100 (MQ=255)
                                                                               gccccccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGt                     >  2:3746627/3‑100 (MQ=38)
                                                                               gccccccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGt                     >  1:3370491/3‑100 (MQ=38)
                                                                                 cccccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                    <  2:1571079/100‑1 (MQ=255)
                                                                                  ccccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa                  >  1:3201407/1‑100 (MQ=39)
                                                                                  ccccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa                  >  1:4170852/1‑100 (MQ=39)
                                                                                  ccccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGttt                   >  1:2646774/1‑100 (MQ=255)
                                                                                  ccccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGAAAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGttt                   >  2:4176296/1‑100 (MQ=255)
                                                                                   cccGGAAGGTGATCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa                  >  1:128200/1‑100 (MQ=255)
                                                                                   cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc                 >  1:4422059/1‑100 (MQ=39)
                                                                                   cccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa                  >  2:1709027/1‑100 (MQ=255)
                                                                                    ccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc                 <  1:3480043/100‑1 (MQ=255)
                                                                                    ccGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc                 <  1:1823560/100‑1 (MQ=255)
                                                                                     cGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACg                <  2:3890570/100‑1 (MQ=255)
                                                                                     cGGAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACg                <  2:4648105/100‑1 (MQ=255)
                                                                                      ggAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGc               >  1:2899442/1‑100 (MQ=255)
                                                                                      ggAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGc               >  1:1085215/1‑100 (MQ=255)
                                                                                      ggAAGATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGc               >  1:2600344/1‑100 (MQ=255)
                                                                                          naTCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAg           >  1:1059244/2‑100 (MQ=255)
                                                                                          gATCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAg           <  1:2624576/100‑1 (MQ=255)
                                                                                           aTCACCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGc          <  2:4578030/100‑1 (MQ=255)
                                                                                              aCCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt       >  1:4621661/1‑100 (MQ=255)
                                                                                              aCCTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt       >  2:1954812/1‑100 (MQ=255)
                                                                                               ccTTCCGGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATg      <  1:3025323/100‑1 (MQ=255)
                                                                                                     |                                                                                                  
CATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGA  >  NC_000913/2090032‑2090228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: