Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,506,193 (A)8→9 coding (907/1119 nt) yjhC → GFO/IDH/MOCA family putative oxidoreductase. NAD(P)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,506,1851.A82.8% 472.9 / 96.8 158coding (899/1119 nt)yjhCGFO/IDH/MOCA family putative oxidoreductase. NAD(P)‑dependent
Reads supporting (aligned to +/- strand):  ref base . (12/15);  new base A (72/58);  total (85/73)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

GCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTATAT  >  NC_000913/4506087‑4506281
                                                                                                   |                                                                                                     
gCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑a                                                                                                     <  1:394295/100‑1 (MQ=255)
gCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑a                                                                                                     <  2:4776268/100‑1 (MQ=255)
 cATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCGTATGGTCATCCAGG‑‑aa                                                                                                    <  2:4908023/100‑1 (MQ=255)
   ttttttGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑aaaa                                                                                                  <  1:11106/100‑1 (MQ=255)
         ggTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑aaaaaaaaac                                                                                          >  2:1237683/1‑98 (MQ=255)
         ggTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑aaaaaaaaac                                                                                          <  1:4474414/100‑3 (MQ=255)
         ggTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑aaaaaaaaac                                                                                          >  1:3789415/1‑98 (MQ=255)
         ggTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑aaaaaaaaac                                                                                          <  1:2029982/100‑3 (MQ=255)
         ggTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑aaaaaaaaaa                                                                                          >  1:4253969/1‑98 (MQ=255)
          gTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAacac                                                                                       >  1:2314873/1‑100 (MQ=255)
          gTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAaaac                                                                                       <  1:1431243/100‑1 (MQ=255)
           tCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAACAAAacac                                                                                       >  1:3334985/1‑100 (MQ=255)
            ccATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑aaaaaaaaacacc                                                                                       <  1:1883705/100‑6 (MQ=255)
            ccATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑aaaaaaaaacacc                                                                                       <  1:2188316/100‑6 (MQ=255)
             cATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCa                                                                                     <  1:2797802/100‑1 (MQ=255)
             cATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAACACCAt                                                                                    >  1:5011645/1‑100 (MQ=255)
              aTGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCAt                                                                                    <  1:634696/100‑1 (MQ=255)
                 aaaCACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTAt                                                                                 >  2:3979049/1‑100 (MQ=255)
                 aaaCACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAACACCATTATg                                                                                <  1:2306869/100‑1 (MQ=255)
                  aaCACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAACACCATTATgg                                                                               <  1:3178973/100‑1 (MQ=255)
                  aaCACAAGAAGAAGATGATGATCGTCGAAAAAGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAAGAAAAAAAAACACCATTATg                                                                                <  2:1562184/100‑1 (MQ=255)
                   acacAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATgg                                                                               >  1:1578002/1‑100 (MQ=255)
                   acacAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAACACCATTATGGc                                                                              <  2:3408588/100‑1 (MQ=255)
                     acaAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCt                                                                             >  1:3746015/1‑100 (MQ=255)
                     acaAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTAAAAAAGAAAAAAAAAACACCATTATGGCt                                                                             <  2:4108321/100‑1 (MQ=255)
                        agaagaagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGc                                                                          >  2:1026778/1‑100 (MQ=255)
                        agaagaagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGc                                                                          >  2:3462094/1‑100 (MQ=255)
                           agaagaTGATGATCGTCGGAAAGGCACTATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCAATATGGCTCGCCAg                                                                       >  2:4742334/1‑100 (MQ=255)
                           agaagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGcccg                                                                       >  1:2943937/1‑98 (MQ=255)
                           agaagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCAAAAAAAAAAAAAACACCATTATGACTTGCCa                                                                        <  2:1214023/100‑1 (MQ=255)
                           agaagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGACATCCAGGAAAAAAAAACACCATTATGGCTTGCCAg                                                                       >  2:2441908/1‑100 (MQ=255)
                            gaagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGt                                                                      <  1:4414340/100‑1 (MQ=255)
                             aagaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGtt                                                                     >  2:1075452/1‑100 (MQ=255)
                              agaTGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACATCATTATGGCTTGCCAGttt                                                                    >  2:1151810/1‑100 (MQ=255)
                               gatgatgatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTta                                                                   >  2:1983187/1‑100 (MQ=255)
                                 tgatgatCGTCGTAAAAGCCATATGACCTCAGAAATGAAGGGCTCTATAGCATATGGTCATACAAG‑‑AAAAAAAACACCATTATGGCTTGCCAGTTtaata                                                                <  2:1419454/100‑1 (MQ=255)
                                 tgatgatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTtaat                                                                 >  2:4510001/1‑100 (MQ=255)
                                   atgatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTtaataa                                                               >  1:1051636/1‑100 (MQ=255)
                                   atgatCGTCGGAAAGGCAATATGAACTCAGAAATGGGTGGCGCTATATCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTtaataa                                                               <  2:3500797/100‑1 (MQ=255)
                                    tgatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAg                                                              >  2:2111126/1‑100 (MQ=255)
                                    tgatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGCTTAATAAg                                                              >  1:480776/1‑100 (MQ=255)
                                     gatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGa                                                             >  2:4534241/1‑100 (MQ=255)
                                     gatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGa                                                             >  2:322069/1‑100 (MQ=255)
                                     gatCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAGAAACACCATTATGGCTTGCCAGTTtaatagaa                                                             >  1:2083208/1‑97 (MQ=255)
                                      atCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCTAGTTTAATAAGaa                                                            >  2:4018111/1‑100 (MQ=255)
                                        cgtcgGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAg                                                          <  1:4603727/100‑1 (MQ=255)
                                         gtcgGAAAGGCAATATGACCTCAGAACTGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAACAAAACACCATTATGGCATGCCATATTTATAAGaacac                                                         >  1:3541151/1‑97 (MQ=255)
                                         gtcgGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAgg                                                         <  1:5036072/100‑1 (MQ=255)
                                          gaggAAAGGCAAAATGACCTCAGAAAAGGATGGCGCTATAGCATATGGTCATCAAGAAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGga                                                        <  2:3900296/98‑1 (MQ=255)
                                           cgGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGgag                                                       <  1:2141111/100‑1 (MQ=255)
                                             gAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGAc                                                     <  2:2911087/100‑1 (MQ=255)
                                               aaGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGt                                                   <  2:3577768/100‑1 (MQ=255)
                                                aGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGtt                                                  >  1:52319/1‑100 (MQ=255)
                                                  gaaaTATGACCTCAGAAATGTATGGCTCTATAGCATATGTTCATCTAGGAAAAAAAAACACCATTATGGCCTGCCAGTTTAATAAGAAAGGAGACGTTAt                                                <  1:4821064/98‑1 (MQ=255)
                                                     atatGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATtcc                                             <  2:2136460/100‑1 (MQ=255)
                                                       atGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATtcctc                                           <  1:2137178/100‑1 (MQ=255)
                                                       atGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCAAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATtcctc                                           <  2:3650143/100‑1 (MQ=255)
                                                       atGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCAAAGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATtcctc                                           <  2:1386149/100‑1 (MQ=255)
                                                        tGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATtcctcc                                          >  2:721037/1‑100 (MQ=255)
                                                        tGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATtcctcc                                          >  2:895047/1‑100 (MQ=255)
                                                         gACCTCAGAAATGGATGGCGCTATAGCATATGAAAAAAAAAAAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCa                                         <  2:1941293/100‑1 (MQ=255)
                                                          aCCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTACTCCTCCat                                        >  2:2995605/1‑100 (MQ=255)
                                                           ccTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTATAATAAAAAAGGTGATATTAATCCTCCaaa                                       >  1:4040536/1‑98 (MQ=255)
                                                           ccTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCcta                                       >  1:2921772/1‑100 (MQ=255)
                                                           ccTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCata                                       >  1:614437/1‑100 (MQ=255)
                                                           ccTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCata                                       <  1:496510/100‑1 (MQ=255)
                                                           ccTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAAAAAGGAGACGTTATTCCTCCata                                       >  1:1971134/1‑100 (MQ=255)
                                                           ccTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCataa                                      >  2:4679806/1‑100 (MQ=255)
                                                           ccTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAAG‑‑AAAAAAAACACCATTATGGCTTCCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCataa                                      >  1:4314589/1‑100 (MQ=255)
                                                             tCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCataat                                     >  2:3784260/1‑100 (MQ=255)
                                                              cAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCataata                                    <  1:4502884/100‑1 (MQ=255)
                                                              cAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAAAAAGAAAGGAGACGTTATTCCTCCataata                                    >  2:81883/1‑100 (MQ=255)
                                                                gAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTCCCAGGTTAATAAGCAAGGGGAGGTTATTTTTACATAATTTc                                  >  1:2641513/1‑100 (MQ=255)
                                                                 aaaTGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATcc                                 <  1:760634/100‑1 (MQ=255)
                                                                 aaaTGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATca                                 >  1:2984884/1‑99 (MQ=255)
                                                                 aaaTGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGGTTAATAAAAAAGGAGACGTTATTCCTCCATAATATcc                                 >  2:1961825/1‑100 (MQ=255)
                                                                  aaTGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGGCAGTTTAATAAAAAAGGAAACATTATTCCTCCAAAATATCct                                >  2:1606305/1‑100 (MQ=255)
                                                                   atggatggCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCctc                               <  1:4023695/100‑1 (MQ=255)
                                                                   atggatggCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCctc                               <  2:3705333/100‑1 (MQ=255)
                                                                     ggatggCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTACCAGTTTAATAAGAAAAGAGACGTAATTCCCCCATCATATCctccg                             >  2:2565723/1‑98 (MQ=255)
                                                                       attgcgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCtgtg                           <  1:4214185/97‑1 (MQ=255)
                                                                        tggCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAACCACCATTATGGCTTGCCAGTTTAATAAGATAGGAGACGTTATTCCTCCATAATATCCTCTgtgg                          >  1:4988910/1‑100 (MQ=255)
                                                                         ggCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTCATAAGAAAGGAGACGTTATTACTCCATAATATACTCtgttgt                         >  1:85246/1‑100 (MQ=255)
                                                                          gcgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTgtggtg                        <  1:4845670/100‑1 (MQ=255)
                                                                           cgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAGAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGc                       <  1:3134173/100‑1 (MQ=255)
                                                                           cgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGATACGTTATTCCTCCATAATATCCTCTGTGGTGc                       >  2:2131332/1‑100 (MQ=255)
                                                                            gcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCa                      >  1:2379917/1‑100 (MQ=255)
                                                                                   cATATGGTCATCCAGGAAAAAAAAACACTATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTg               <  1:4680986/100‑1 (MQ=255)
                                                                                     tatGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCATTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCTTCTGTGGTGCAAAACCTgaa             >  2:2805896/1‑100 (MQ=255)
                                                                                     tatGATCATCCAGG‑‑AAAAAAAAGACCCATTATGGCTTGCCAGTTTAATAAGAATGTAGACGTTATTCCTCCATAATAGCCTGTGTGGTGCAAAACCTgaa             <  2:4868763/100‑1 (MQ=255)
                                                                                      atGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTgaag            <  1:3639122/100‑1 (MQ=255)
                                                                                      atGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTgaag            <  1:2590311/100‑1 (MQ=255)
                                                                                       tGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTgaaga           >  2:3162209/1‑100 (MQ=255)
                                                                                       tGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTgaaga           >  2:1336575/1‑100 (MQ=255)
                                                                                        ggTCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTgaagaa          >  1:76044/1‑100 (MQ=255)
                                                                                          tCATCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAagaaga        <  1:1177813/100‑1 (MQ=255)
                                                                                           cagccAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATACCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGAt       <  1:3500610/97‑1 (MQ=255)
                                                                                            aTCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGAtt      >  1:2985815/1‑100 (MQ=255)
                                                                                            aTCCAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGAtt      >  1:1653010/1‑100 (MQ=255)
                                                                                              ccAGGAAAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATtat    >  2:4986086/1‑100 (MQ=255)
                                                                                                   |                                                                                                     
GCATTTTTTGGTCCATGAAACACAAGAAGAAGATGATGATCGTCGGAAAGGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑‑AAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTATAT  >  NC_000913/4506087‑4506281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: