Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,461,3240AG37.1% 191.8 / 248.4 240D101G (GAT→GGT) gspGpseudopilin, cryptic, general secretion pathway
Reads supporting (aligned to +/- strand):  ref base A (69/80);  new base G (45/43);  total (117/123)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 242 total aligned reads displayed.

CCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATGGGAACCGAGGACG  >  NC_000913/3461225‑3461422
                                                                                                   |                                                                                                  
ccACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGa                                                                                                    >  1:2235012/1‑100 (MQ=255)
 cacaAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGAt                                                                                                   <  2:3981392/100‑1 (MQ=255)
 cacaAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGAt                                                                                                   <  1:721555/100‑1 (MQ=255)
   caAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTcc                                                                                                 >  2:1808028/1‑100 (MQ=255)
   caAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATcc                                                                                                 >  1:4904761/1‑100 (MQ=255)
    aaaTCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTccc                                                                                                >  1:3435141/1‑100 (MQ=255)
    aaaTCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATccc                                                                                                <  1:2842920/100‑1 (MQ=255)
    aaaTCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATccc                                                                                                >  2:219592/1‑100 (MQ=255)
      aTCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTg                                                                                              <  2:3376991/100‑1 (MQ=255)
      aTCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTg                                                                                              <  1:1523630/100‑1 (MQ=255)
         aGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTgggg                                                                                           <  1:2134959/100‑1 (MQ=255)
          ggggCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGc                                                                                          >  2:2902486/1‑100 (MQ=255)
          ggggCTTGAATCCTTAGTAGAAGAGCCGACACTGACACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAACCGCCTGCGTGCCGGTCCCAGGGGc                                                                                          >  2:1486275/1‑100 (MQ=255)
             gCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAAt                                                                                       >  1:2444157/1‑100 (MQ=255)
             gCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAAt                                                                                       >  2:2220887/1‑100 (MQ=255)
              ctcgAATCCTCAGTAGAAGCGCCGACACTGCCACCGCTGACCGCTAACTATAACAAGGAAGGTTATATCCAGCTACTGGCTGCCGCTACCTGGGGCAATg                                                                                      >  2:4679775/4‑100 (MQ=255)
              cTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATg                                                                                      <  1:3094623/100‑1 (MQ=255)
              cTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATg                                                                                      <  1:2730919/100‑1 (MQ=255)
              cTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATg                                                                                      >  2:4469124/1‑100 (MQ=255)
                    tCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGTAGGTTATATCAAGCGTCTGCCCGCCGATCCCTGGGGCAATGATTATg                                                                                <  1:4616407/100‑1 (MQ=255)
                          gtagAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGCAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCg                                                                          >  2:580292/1‑100 (MQ=255)
                          gtagAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCg                                                                          <  2:2208266/100‑1 (MQ=255)
                           tagAAGCGCCGACACTGCCACCGCGGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGt                                                                         <  2:827025/100‑1 (MQ=255)
                            agAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGtt                                                                        >  2:2271598/1‑100 (MQ=255)
                            agAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGtt                                                                        >  1:1347297/1‑100 (MQ=255)
                            agAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGtt                                                                        <  1:2681657/100‑1 (MQ=255)
                            agAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGtt                                                                        >  1:2966246/1‑100 (MQ=255)
                            agAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGtt                                                                        <  1:1536073/100‑1 (MQ=255)
                             gAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTa                                                                       >  1:1789415/1‑100 (MQ=255)
                             gAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTa                                                                       >  1:4450930/1‑100 (MQ=255)
                                 cgcCGAGACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCTTCTGCCTGCCGGTCGCTGGGGCAATGATTATGTCCTCGGTAATcc                                                                   >  2:4778755/1‑100 (MQ=255)
                                  gcCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCt                                                                  >  2:3087312/1‑100 (MQ=255)
                                   ccGACACCGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTg                                                                 <  1:4229259/100‑1 (MQ=255)
                                    cGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTgg                                                                <  2:552975/100‑1 (MQ=255)
                                    cGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATCATGTCCTCGTTAATCCTgg                                                                >  2:1277262/1‑100 (MQ=255)
                                      acacTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTg                                                              <  1:3801982/100‑1 (MQ=255)
                                        acagCCACCACATGCCTCAAACAACAACAAGAAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGaa                                                            <  2:1502496/97‑1 (MQ=255)
                                          tGCCACCGCTGGCCGCAAACAATACCAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGTTGAACa                                                          <  2:80842/100‑1 (MQ=255)
                                           gCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCCGGTGAACAt                                                         >  2:1214553/1‑100 (MQ=255)
                                           gCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACAt                                                         >  1:478926/1‑100 (MQ=255)
                                           gCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACAt                                                         >  2:5054708/1‑100 (MQ=255)
                                           gCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACAt                                                         <  1:3122234/100‑1 (MQ=255)
                                           gCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGGCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACAt                                                         >  1:3467701/1‑100 (MQ=255)
                                            ccACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCTTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATg                                                        >  1:31832/1‑100 (MQ=255)
                                             cACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATgg                                                       <  1:2507749/100‑1 (MQ=255)
                                             cACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATgg                                                       >  2:735020/1‑100 (MQ=255)
                                              aCCGCTGGCCGCAAACTATAACAAGGGAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGt                                                      <  2:2569317/100‑1 (MQ=255)
                                               ccGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTg                                                     >  1:4709506/1‑100 (MQ=255)
                                                 gCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCa                                                   >  2:3634396/1‑100 (MQ=255)
                                                  cTGGCGGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCAt                                                  <  2:4246231/100‑1 (MQ=255)
                                                  cTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCAt                                                  >  1:2383491/1‑100 (MQ=255)
                                                   tGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATa                                                 <  2:472347/100‑1 (MQ=255)
                                                   tGGCCGCAAACTATAACAAGGAAGGTTATATCAAACGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATa                                                 <  2:3605334/100‑1 (MQ=255)
                                                    ggCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATAc                                                >  2:879626/1‑100 (MQ=255)
                                                    ggCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATAc                                                >  1:1945304/1‑100 (MQ=255)
                                                      acccAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGCTCCCTGGGGCAACGATTATCTCCTCGTTAATCCTGATGAACAAGGTGCACACGa                                              >  2:1035259/4‑100 (MQ=255)
                                                       cGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGAt                                             <  2:3513530/100‑1 (MQ=255)
                                                        gCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATc                                            <  1:1831742/100‑1 (MQ=255)
                                                         cAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCt                                           >  2:69927/1‑100 (MQ=255)
                                                         cAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCt                                           >  1:760157/1‑100 (MQ=255)
                                                          aaaCTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTg                                          >  1:3379123/1‑100 (MQ=255)
                                                            aCTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCt                                        <  2:1765977/100‑1 (MQ=255)
                                                            aCTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCt                                        >  1:5040476/1‑100 (MQ=255)
                                                            aCTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCt                                        >  2:3664781/1‑100 (MQ=255)
                                                            aCTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCt                                        <  1:3774191/100‑1 (MQ=255)
                                                            aCTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCt                                        <  1:1208340/100‑1 (MQ=255)
                                                             cTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCtt                                       <  1:1695192/100‑1 (MQ=255)
                                                               ataACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTc                                     >  1:1993667/1‑100 (MQ=255)
                                                               ataACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTc                                     <  2:2415587/100‑1 (MQ=255)
                                                               ataACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCGCTGGGGCAATGATTATTTCCTCGTTAATACTGGTGAGCATGGTGCATACTATCTGCTTTc                                     >  2:2530306/1‑100 (MQ=255)
                                                               ataACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTc                                     <  1:893739/100‑1 (MQ=255)
                                                               ataACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTc                                     >  2:4581184/1‑100 (MQ=255)
                                                                taACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTca                                    >  2:2065825/1‑100 (MQ=255)
                                                                taACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTca                                    <  1:1484175/100‑1 (MQ=255)
                                                                 aaCAAGGAAGGTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTcag                                   <  2:1366365/100‑1 (MQ=255)
                                                                  aCAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTcagc                                  <  2:1326482/100‑1 (MQ=255)
                                                                   cAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTcagca                                 >  2:2937677/1‑100 (MQ=255)
                                                                    aaggaaggTTATATCAAGCGTCTGCATGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTcagcag                                <  1:2516226/100‑1 (MQ=255)
                                                                      ggaaggTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAggg                              <  1:2298871/100‑1 (MQ=255)
                                                                       gaaggTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGc                             <  1:650040/100‑1 (MQ=255)
                                                                       gaaggTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGACCATGGTGCATACGATCTGCTTTCAGCAGGGc                             >  1:5118046/1‑100 (MQ=255)
                                                                         aggTTAGATCAAGCGTCTGCCTGCCGGTCCTTGGGGCAATGATTATGTCCGCGTTAATCCTGGTGAACATGGGGCATACGATCTGCTTTCAGCAGTGccc                           <  2:4785690/100‑1 (MQ=255)
                                                                           gTTATATCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGa                         >  2:3193385/1‑100 (MQ=255)
                                                                             tataTCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGTCCCGATg                       <  2:4187787/100‑1 (MQ=255)
                                                                               tatCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGt                     >  1:1130188/1‑100 (MQ=255)
                                                                                atCAAGCGTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTg                    >  1:1719637/1‑100 (MQ=255)
                                                                                 tCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGa                   >  1:3985954/1‑100 (MQ=255)
                                                                                  cAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGaa                  >  1:1204623/1‑100 (MQ=255)
                                                                                  cAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGaa                  <  1:3213577/100‑1 (MQ=255)
                                                                                   aaGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGaaa                 >  2:3161258/1‑100 (MQ=255)
                                                                                      cGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATgg              <  2:1871143/100‑1 (MQ=255)
                                                                                       gTCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATggg             >  1:1889927/1‑100 (MQ=255)
                                                                                        tCTGCCTGCCGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATGGGa            <  2:1605493/100‑1 (MQ=255)
                                                                                        tCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATGGGa            <  1:1582/100‑1 (MQ=255)
                                                                                         ctgcctgcCGATCCCTGGGGCAATGATTATGTCCTCGTTAATACTGGTGAACATCGTGCATACGATCTGTTTTCAGCAGGGCCCGATGGTGAAATGGGaa           <  1:4483149/100‑1 (MQ=255)
                                                                                            cctgccGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATGGGAACCg        >  2:3429899/1‑100 (MQ=255)
                                                                                             ctgccGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATGGGAACCGa       >  2:4941956/1‑100 (MQ=255)
                                                                                              tgccGGTCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATGGGAACCGAg      <  1:2873473/100‑1 (MQ=255)
                                                                                                ccGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTAACCATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATGGGAACCGAGga    >  2:4837012/1‑100 (MQ=255)
                                                                                                  gATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATGGGAACCGAGgacg  >  1:842742/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
CCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACTGCCACCGCTGGCCGCAAACTATAACAAGGAAGGTTATATCAAGCGTCTGCCTGCCGATCCCTGGGGCAATGATTATGTCCTCGTTAATCCTGGTGAACATGGTGCATACGATCTGCTTTCAGCAGGGCCCGATGGTGAAATGGGAACCGAGGACG  >  NC_000913/3461225‑3461422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑