Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 13,957 A→T M599L (ATG→TTG)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091313,9570AT95.1% 671.7 / 19.9 205M599L (ATG→TTG) dnaKchaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base A (6/4);  new base T (116/78);  total (123/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 205 total aligned reads displayed.

GTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTT  >  NC_000913/13860‑14055
                                                                                                 |                                                                                                  
gTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTg                                                                                                  <  2:3528999/100‑1 (MQ=255)
 tCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTgg                                                                                                 >  1:2386008/1‑100 (MQ=255)
  cTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGa                                                                                                >  1:85137/1‑100 (MQ=255)
  cTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGACGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCACAAACTGTTGGa                                                                                                >  1:4448297/1‑100 (MQ=255)
   tGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGaa                                                                                               >  1:4921302/1‑100 (MQ=255)
   tGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGaa                                                                                               <  1:410758/100‑1 (MQ=255)
   tGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGaa                                                                                               >  1:2322429/1‑100 (MQ=255)
   tGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGaa                                                                                               >  1:2152944/1‑100 (MQ=255)
    gcgcTGACTGCACTGGAAACTGTTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGaaa                                                                                              <  1:4463642/100‑1 (MQ=255)
    gcgcTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCGCAGGTTTCCCAGAAACTGTTGGaaa                                                                                              >  1:4048823/1‑100 (MQ=255)
    gcgcTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGaaa                                                                                              >  1:3989849/1‑100 (MQ=255)
     cgcTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAAt                                                                                             >  1:1436211/1‑100 (MQ=255)
        tGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGc                                                                                          <  1:2833231/100‑1 (MQ=255)
        tGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGc                                                                                          <  1:2049292/100‑1 (MQ=255)
        tGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGc                                                                                          <  2:2704914/100‑1 (MQ=255)
         gACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGcc                                                                                         <  2:450591/100‑1 (MQ=255)
           cTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCca                                                                                       >  2:2026735/1‑100 (MQ=255)
             ncACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCcagc                                                                                     >  1:28098/2‑100 (MQ=255)
             gCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCcagc                                                                                     >  1:1495198/1‑100 (MQ=255)
             gCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCcagc                                                                                     <  1:3169484/100‑1 (MQ=255)
             gCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCcagc                                                                                     >  1:4699168/1‑100 (MQ=255)
              cACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCcagca                                                                                    <  1:2543778/100‑1 (MQ=255)
                cTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCagcagc                                                                                  >  2:5035187/1‑100 (MQ=255)
                cTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCagcagc                                                                                  >  2:5014195/1‑100 (MQ=255)
                  ggAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCaa                                                                                >  1:807998/1‑100 (MQ=255)
                  ggAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCaa                                                                                >  1:3409674/1‑100 (MQ=255)
                   gAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAAc                                                                               <  2:2215877/100‑1 (MQ=255)
                   gAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAAc                                                                               <  1:1681265/100‑1 (MQ=255)
                    aaaCTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACa                                                                              >  1:1789168/1‑100 (MQ=255)
                       cTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGc                                                                           >  2:277644/1‑100 (MQ=255)
                       cTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGc                                                                           >  1:1914329/1‑100 (MQ=255)
                         gCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGccc                                                                         >  2:3173776/1‑100 (MQ=255)
                          ctctGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCca                                                                        >  1:581374/1‑100 (MQ=255)
                          ctctGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCca                                                                        <  1:700136/100‑1 (MQ=255)
                          ctatGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCca                                                                        >  2:3823645/4‑100 (MQ=255)
                           tctGAAAGGTGAAGACAAAACCGCTACCGAAGCGATAATGCAGGAACTGGCACAGGTTTCCCATAAACTATTGGAGAACGCCCAGCAGTTACATGCCcag                                                                       >  2:2886963/1‑100 (MQ=255)
                            ctGAAAGGTGAAGTCAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGTACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCcagc                                                                      <  1:4648521/100‑1 (MQ=255)
                              gAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCcagccg                                                                    >  2:3002484/1‑98 (MQ=255)
                              gAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCcagcag                                                                    <  2:3646195/100‑1 (MQ=255)
                              gAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCcagcag                                                                    >  2:3746432/1‑100 (MQ=255)
                              gAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCcagcag                                                                    >  1:135839/1‑100 (MQ=255)
                                 aGGTGAAGACAAAGCCGCTATCGAAGCGATAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCcagcagcag                                                                 <  1:4715468/100‑1 (MQ=255)
                                 aGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCcagcagcag                                                                 <  1:299730/100‑1 (MQ=255)
                                  ggTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGa                                                                <  2:3977098/100‑1 (MQ=255)
                                   gTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGAc                                                               <  2:4347954/100‑1 (MQ=255)
                                   gTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGAc                                                               <  2:1404388/100‑1 (MQ=255)
                                    tGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACt                                                              >  1:2917803/1‑100 (MQ=255)
                                     gAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTg                                                             >  1:638168/1‑100 (MQ=255)
                                     gAAGACAAAGCCGCAATCGAAGCGAAAATGCAGCAACTGGCACAGGATTCCCAGAAACTGCTATAAATCTCCCAGCAGCAACATGCCCGGCAGAAGCCTg                                                             >  2:370712/1‑100 (MQ=255)
                                        gACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCg                                                          >  2:318774/1‑100 (MQ=255)
                                         aCAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCATACTGCCgg                                                         <  2:4865331/100‑1 (MQ=255)
                                          cAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGt                                                        >  1:5055756/1‑100 (MQ=255)
                                          cAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGt                                                        <  2:83931/100‑1 (MQ=255)
                                            aaGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGc                                                      <  1:46805/100‑1 (MQ=255)
                                              gCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTg                                                    <  2:4983283/100‑1 (MQ=255)
                                               tctcTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGa                                                   <  1:3217103/97‑1 (MQ=255)
                                               ccGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGa                                                   >  2:4718449/1‑100 (MQ=255)
                                                 gCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATg                                                 <  2:1473677/100‑1 (MQ=255)
                                                  cTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGc                                                <  1:729110/100‑1 (MQ=255)
                                                  cTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCACCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGc                                                >  2:5011747/1‑100 (MQ=255)
                                                   tATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCt                                               <  1:986250/100‑1 (MQ=255)
                                                    aTCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCtt                                              >  1:4139717/1‑100 (MQ=255)
                                                     tCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTc                                             <  1:3308267/100‑1 (MQ=255)
                                                     tCGAAGCGAAAATGCAGGAACTGGCACAGGGTTCCCAGAAACTGTTGGAAATCGCCCCGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTc                                             <  1:4384027/100‑1 (MQ=255)
                                                     tCGAAGCGAAAATGCAGGAACTGACACAGGTTGCCCAGAAACTATTGGAAAACGCCCAGCAGCAACATGCCCAGCAGCAGACTACCGGGGCCGCTCCTTc                                             >  2:4250792/1‑100 (MQ=255)
                                                      cGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCt                                            <  2:3434668/100‑1 (MQ=255)
                                                      cGAAGCGAAAAGGCAGGAACTGGCACAGGTTTCCCAGAAGCTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCt                                            <  2:2843417/100‑1 (MQ=255)
                                                       gAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTg                                           <  2:16224/100‑1 (MQ=255)
                                                          gCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAATTCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCaa                                        <  2:1088511/100‑1 (MQ=255)
                                                          gCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCaa                                        >  1:4079016/1‑100 (MQ=255)
                                                            gAAGATGCAGGAACTGGCACAGGTTTCCCAGAAGCTGTTGGAAATCGCCCAGCCGCCACATACCCCGCAGCAGACTGCCGATGCTGATGTTTCTGCAaac                                      >  2:4890287/1‑100 (MQ=255)
                                                              aaaTGCGGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAaacaa                                    >  2:642969/1‑100 (MQ=255)
                                                              aaaTGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAaacaa                                    <  1:1180669/100‑1 (MQ=255)
                                                                aTGCAGGAACTGCCACAGGTTTCCCAGAAACTGTTGGAAGTCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCGGATGCTTCTGCAAACAAcg                                  <  2:756956/100‑1 (MQ=255)
                                                                 tGCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAAcgc                                 <  1:1986484/100‑1 (MQ=255)
                                                                  gCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAAcgcc                                <  1:5114344/100‑2 (MQ=255)
                                                                  gCAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAAcgcg                                >  1:3616126/1‑100 (MQ=255)
                                                                   cAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGa                               >  2:3365097/1‑100 (MQ=255)
                                                                   cAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGa                               >  2:2851087/1‑100 (MQ=255)
                                                                   cAGGAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGa                               >  1:3690743/1‑100 (MQ=255)
                                                                     ggAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGaaa                             >  1:1897675/1‑100 (MQ=255)
                                                                     ggAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGaaa                             >  2:1603585/1‑100 (MQ=255)
                                                                     ggAACTGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTATGCAAACAACGCGaaa                             >  1:4239419/1‑100 (MQ=255)
                                                                          tGGCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGa                        >  1:1213904/1‑100 (MQ=255)
                                                                           ggCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGAc                       >  1:528733/1‑100 (MQ=255)
                                                                           ggCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGAc                       <  1:3586887/100‑1 (MQ=255)
                                                                            gCACAGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACg                      <  2:1241619/100‑1 (MQ=255)
                                                                               caGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGAtg                   >  2:4702610/1‑100 (MQ=255)
                                                                               caGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGAtg                   <  1:2867983/100‑1 (MQ=255)
                                                                                aGGTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGAtgt                  <  1:1039426/100‑1 (MQ=255)
                                                                                 ggTTTCCCAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGAtgtt                 <  1:5091997/100‑1 (MQ=255)
                                                                                      cccAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGa            >  1:383641/1‑100 (MQ=255)
                                                                                      cccAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGa            >  2:3583839/1‑100 (MQ=255)
                                                                                        cAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACg          <  2:3945939/100‑1 (MQ=255)
                                                                                        cAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACg          >  2:1969589/1‑100 (MQ=255)
                                                                                        cAGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACg          <  2:1961074/100‑1 (MQ=255)
                                                                                         aGAAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGc         >  2:2309587/1‑100 (MQ=255)
                                                                                            aaCTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGa      >  2:4393498/1‑100 (MQ=255)
                                                                                              cTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAAt    >  2:3264833/1‑100 (MQ=255)
                                                                                                gATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAAttt  >  2:2782359/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
GTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTT  >  NC_000913/13860‑14055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: