BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	706,591	G→A	E167K (GAA→AAA)	glnS →	glutamyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	706,591	0	G	A	99.4%	612.1 / NA	176	E167K (GAA→AAA)	glnS	glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (75/99); total (75/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

CGGCACCCTGACGCAACCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCTGAAC  >  NC_000913/706494‑706687

cgGCAGCCTGACGCAACCGGGTAAAAACAGCGCGTACCGCGACCGCAGCGTTGAATAGAACCTGGCGCTGGTCGTAAAAATGCGTGCCGGTGGTTTTaaa                                                                                                <  2:3719466/100‑1 (MQ=255)

cgGCACCCTGACGCAACCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTaaa                                                                                                <  2:3227110/100‑1 (MQ=255)

 ggCACCCTGACGCAACCGGGTAAACACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTaaag                                                                                               <  1:2338111/100‑1 (MQ=255)

    aCCCTGACGCAACCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAaagaag                                                                                            <  1:922374/100‑1 (MQ=255)

     cccTGACGCAACCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAgg                                                                                           <  2:2197406/100‑1 (MQ=255)

     cccTGACGCAACCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAgg                                                                                           >  1:106991/1‑100 (MQ=255)

       cTGACGCAACCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTGGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTa                                                                                         <  2:1594083/100‑1 (MQ=255)

        tGACGCAACCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGAGATGTAAGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTaa                                                                                        <  2:4313078/100‑1 (MQ=255)

          aCGCAACCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAg                                                                                      >  1:5122012/1‑100 (MQ=255)

              aaCCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAgcctg                                                                                  <  1:1834097/100‑1 (MQ=255)

              aaCCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAgcctg                                                                                  <  1:4517462/100‑1 (MQ=255)

                ccGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAgcctgcc                                                                                <  1:3297840/100‑1 (MQ=255)

                ccGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAgcctgcc                                                                                <  1:2721880/100‑1 (MQ=255)

                  gggTAAAAACAGCCCGTACCGCGACCGCAGCTTTGAAGAGTACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGcctgcctg                                                                              <  2:539581/100‑1 (MQ=255)

                  gggTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGcctgcctg                                                                              <  1:3568616/100‑1 (MQ=255)

                    gTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCtgcg                                                                            >  2:3856534/1‑100 (MQ=255)

                    gTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCtgcg                                                                            >  2:1309374/1‑100 (MQ=255)

                       aaaaCAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCtgcgtgc                                                                         <  2:3631541/100‑1 (MQ=255)

                        aaaCAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCtgcgtgcg                                                                        >  2:800084/1‑100 (MQ=255)

                         aaCAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGa                                                                       >  2:1143860/1‑100 (MQ=255)

                             gCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAAt                                                                   <  1:3329850/100‑1 (MQ=255)

                             gCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAAt                                                                   <  2:4933612/100‑1 (MQ=255)

                             gCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGAAAAGCCTGCCTGCGTGCGAAAAt                                                                   >  1:3217420/1‑100 (MQ=255)

                              cccGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATc                                                                  <  1:848616/100‑1 (MQ=255)

                              cccGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATc                                                                  >  1:2515593/1‑100 (MQ=255)

                              cccGGACCGCGACCGCTGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATc                                                                  <  1:4752101/100‑1 (MQ=255)

                               ccGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCg                                                                 >  1:195275/1‑100 (MQ=255)

                               ccGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGCTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCg                                                                 >  1:3730894/1‑100 (MQ=255)

                                 gTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGAc                                                               <  1:2153227/100‑1 (MQ=255)

                                 gTACCGCGACCGCAGCGTTGAAGAGAACCTGGAGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTACCTGCGTGCGAAAATCGAc                                                               >  1:4585391/1‑100 (MQ=255)

                                  tACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACa                                                              <  2:1545592/100‑1 (MQ=255)

                                   aCCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACAt                                                             <  1:3988568/100‑1 (MQ=255)

                                    ccGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATg                                                            <  2:4461071/100‑1 (MQ=255)

                                    ccGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGGGGTTGTAAAGAAGGGAAAGCCTGCCTGCGTGCGAAAATCGACATg                                                            >  2:2374283/1‑100 (MQ=255)

                                     cgcgACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATgg                                                           >  2:4069877/1‑100 (MQ=255)

                                      gcgACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGc                                                          <  2:2704450/100‑1 (MQ=255)

                                      gcgACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGc                                                          <  2:3011679/100‑1 (MQ=255)

                                          ccGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCa                                                      <  2:1714055/100‑1 (MQ=255)

                                          ccGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCa                                                      >  2:1898609/1‑100 (MQ=255)

                                          ccGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCa                                                      <  1:290966/100‑1 (MQ=255)

                                           cGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCAc                                                     >  2:2100170/1‑100 (MQ=255)

                                            gCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCAcc                                                    <  2:4543552/100‑1 (MQ=255)

                                            gCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCAcc                                                    >  2:1010507/1‑100 (MQ=255)

                                            gCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCAcc                                                    >  1:1405018/1‑100 (MQ=255)

                                             cAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCg                                                   >  2:4760205/1‑100 (MQ=255)

                                               gCGTTGAAGAGAACCTGGCGCTGTTCGAAACAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGAAATGGCTTCACCGtt                                                 <  2:4828186/100‑1 (MQ=255)

                                               gCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGGGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGtt                                                 >  2:4949072/1‑100 (MQ=255)

                                               gCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGtt                                                 <  1:4635521/100‑1 (MQ=255)

                                                   tGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATc                                             <  1:4643189/100‑1 (MQ=255)

                                                   tGAAGAGAACCTGGCGCTGCTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATc                                             >  2:4403522/1‑100 (MQ=255)

                                                    gAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGAAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCg                                            <  2:3181513/100‑1 (MQ=255)

                                                      agagAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTg                                          <  1:5025706/100‑1 (MQ=255)

                                                      agagAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTg                                          >  2:4210238/1‑100 (MQ=255)

                                                       gagaACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGa                                         <  2:2685788/100‑1 (MQ=255)

                                                        agaACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGAt                                        <  2:1491087/100‑1 (MQ=255)

                                                         gaACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATg                                       >  1:490187/1‑100 (MQ=255)

                                                          aaCCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATgc                                      <  2:2855120/100‑1 (MQ=255)

                                                            ccTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATgcgc                                    >  1:2697019/1‑100 (MQ=255)

                                                             ntGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGcgcg                                   >  1:4099518/2‑100 (MQ=255)

                                                              tGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGa                                  >  1:110853/1‑100 (MQ=255)

                                                               ggCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGAt                                 >  1:5068908/1‑100 (MQ=255)

                                                               ggCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGAt                                 >  2:4577874/1‑100 (MQ=255)

                                                                 cgcTGTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATcc                               <  1:248610/100‑1 (MQ=255)

                                                                     gTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTg                           <  1:1203770/100‑1 (MQ=255)

                                                                     gTTCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTg                           >  2:2899105/1‑100 (MQ=255)

                                                                      ttCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGc                          <  2:1933398/100‑1 (MQ=255)

                                                                      ttCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGc                          <  2:2081167/100‑1 (MQ=255)

                                                                      ttCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGc                          <  1:3210161/100‑1 (MQ=255)

                                                                       tCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGcc                         <  2:660549/100‑2 (MQ=255)

                                                                       tCGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCt                         >  1:1022228/1‑100 (MQ=255)

                                                                        cGAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTg                        >  1:213115/1‑100 (MQ=255)

                                                                         gAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGt                       >  1:1758825/1‑100 (MQ=255)

                                                                         gAAAAAATGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGt                       >  1:3840939/1‑100 (MQ=255)

                                                                           aaaaaTGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTAc                     >  2:1219889/1‑100 (MQ=255)

                                                                            aaaaTGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTAcc                    >  1:5072544/1‑100 (MQ=255)

                                                                             aaaTGCGTGCCGGTGGTTTTAAAGAAGGTAAATCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCg                   <  2:104516/100‑1 (MQ=255)

                                                                             aaaTGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCg                   <  2:2587958/100‑1 (MQ=255)

                                                                             aaaTGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCg                   <  1:1903765/100‑1 (MQ=255)

                                                                             aaaTGCGTGCCGGTGGTGTTAAAGAAGGTAAAGCCGGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTCCTGTACCg                   <  2:4876044/100‑1 (MQ=255)

                                                                              aaTGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGt                  >  2:396565/1‑100 (MQ=255)

                                                                                tGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTAt                <  1:766051/100‑1 (MQ=255)

                                                                                tGCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTAt                <  1:4476887/100‑1 (MQ=255)

                                                                                 gCGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTAtt               <  1:4631610/100‑1 (MQ=255)

                                                                                  cGTGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTa              >  1:4460570/1‑100 (MQ=255)

                                                                                   gTGCCGGTGCTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACCTGGCTTCACCCTTTATCGTGATGCCCGCTCCGGTGCCGTCCCGTACTaa             >  2:216502/1‑100 (MQ=255)

                                                                                    tGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTaaa            <  1:2736590/100‑1 (MQ=255)

                                                                                    tGCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTaaa            <  1:3170256/100‑1 (MQ=255)

                                                                                     gCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAGTCGACATGGCTTCACCGTTTATCGTGATGCGCGATCTGGTGCTGTACCGTATTAAAt           <  2:2200960/100‑1 (MQ=255)

                                                                                     gCCGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAAt           <  2:3588685/100‑1 (MQ=255)

                                                                                      ccGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAAtt          <  1:675749/100‑1 (MQ=255)

                                                                                       cGGTGGTTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCACGATCCGGTGCTGTACCGTATTAAAttt         >  2:2626834/1‑100 (MQ=255)

                                                                                        ggtggtTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTg        >  1:3106796/1‑100 (MQ=255)

                                                                                         gtggtTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGc       <  2:109397/100‑1 (MQ=255)

                                                                                          tggtTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCt      <  2:2296327/100‑1 (MQ=255)

                                                                                          tggtTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCt      <  1:1106567/100‑1 (MQ=255)

                                                                                           ggtTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAAGCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCTg     <  1:216367/100‑1 (MQ=255)

                                                                                            gtTTTAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCTGa    >  1:225047/1‑100 (MQ=255)

                                                                                             ttttAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCTGaa   >  2:3750463/1‑100 (MQ=255)

                                                                                             ttttAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCTGaa   <  2:1082264/100‑1 (MQ=255)

                                                                                              tttAAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCTGAac  >  1:1007710/1‑100 (MQ=255)

CGGCACCCTGACGCAACCGGGTAAAAACAGCCCGTACCGCGACCGCAGCGTTGAAGAGAACCTGGCGCTGTTCGAAAAAATGCGTGCCGGTGGTTTTGAAGAAGGTAAAGCCTGCCTGCGTGCGAAAATCGACATGGCTTCACCGTTTATCGTGATGCGCGATCCGGTGCTGTACCGTATTAAATTTGCTGAAC  >  NC_000913/706494‑706687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`