BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	714,588	C→T	A344V (GCC→GTC)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	714,588	0	C	T	97.9%	631.0 / ‑0.9	188	A344V (GCC→GTC)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand): ref base C (3/1); new base T (109/75); total (112/76)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

CGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTT  >  NC_000913/714493‑714684

cGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTgtcgtc                                                                                              >  1:3571600/1‑100 (MQ=255)

cGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTgtcgtc                                                                                              <  2:519298/100‑1 (MQ=255)

cGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTgtcgtc                                                                                              <  2:3534117/100‑1 (MQ=255)

cGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTgtcgtc                                                                                              >  1:261539/1‑100 (MQ=255)

  tCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCgg                                                                                            <  1:2235456/100‑1 (MQ=255)

   cACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGt                                                                                           >  2:3746902/1‑100 (MQ=255)

     cTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaa                                                                                         >  1:3988466/1‑100 (MQ=255)

     cTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaa                                                                                         >  2:343068/1‑100 (MQ=255)

      tCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaaa                                                                                        <  1:1837289/100‑1 (MQ=255)

       ccGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaaaa                                                                                       >  2:1048461/1‑100 (MQ=255)

       ccGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaaaa                                                                                       >  2:4004270/1‑100 (MQ=255)

       ccGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTCTCGTCGGTaaaa                                                                                       <  1:4432424/100‑1 (MQ=255)

        cGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAAc                                                                                      >  1:1330876/1‑100 (MQ=255)

         ggCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACg                                                                                     >  2:2686502/1‑100 (MQ=255)

         ggCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACg                                                                                     >  2:2661649/1‑100 (MQ=255)

                ttGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGttt                                                                              <  2:4766847/100‑1 (MQ=255)

                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            <  2:1310137/100‑1 (MQ=255)

                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            <  1:1436606/100‑1 (MQ=255)

                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            >  2:348920/1‑100 (MQ=255)

                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            >  1:330455/1‑100 (MQ=255)

                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            <  1:2877358/100‑1 (MQ=255)

                    tgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCCAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATc                                                                          >  2:4465868/1‑100 (MQ=255)

                    tgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATc                                                                          <  1:4740948/100‑1 (MQ=255)

                    tgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATc                                                                          >  2:1747774/1‑100 (MQ=255)

                      aaTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGCTGTTGTCGTCGGTAAAACGCTGGTTTCATCTg                                                                        >  2:3617511/1‑100 (MQ=255)

                       aTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATcgca                                                                       >  2:2320349/1‑97 (MQ=255)

                       aTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGc                                                                       <  1:307810/100‑1 (MQ=255)

                       aTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGc                                                                       >  1:5105222/1‑100 (MQ=255)

                       aTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGc                                                                       <  1:641407/100‑1 (MQ=255)

                          cGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCgat                                                                    <  2:2070948/100‑1 (MQ=255)

                          cGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCgac                                                                    >  2:3001730/1‑99 (MQ=255)

                          cGAACCACTACCTGGCGGTGGCAATCAATCACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCgat                                                                    <  1:4707521/100‑1 (MQ=255)

                          cGAACCACGACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCgat                                                                    <  2:3555793/100‑1 (MQ=255)

                              ccACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATc                                                                >  1:1741231/1‑100 (MQ=255)

                              ccACTACCTGGCGGTGGCAATCAATAAACTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTACAACGCTGGTTTCATCTGCGATGATc                                                                >  2:186430/1‑100 (MQ=255)

                               cACTACCTGGCGGTGGCGATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTTGGTAAAACGCTGGTTTCTTCTGCGATGATCg                                                               >  2:314392/1‑100 (MQ=255)

                               cACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCg                                                               >  1:1886861/1‑100 (MQ=255)

                                aCTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGa                                                              >  1:444604/1‑100 (MQ=255)

                                aCTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGa                                                              >  2:2383776/1‑100 (MQ=255)

                                  tACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGAcc                                                            <  2:7673/100‑1 (MQ=255)

                                   aCCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCg                                                           >  1:4316401/1‑100 (MQ=255)

                                     cTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCgtg                                                         >  1:2016028/1‑100 (MQ=255)

                                      tGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCgtgt                                                        <  2:3843096/100‑1 (MQ=255)

                                       ggCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGtgtg                                                       <  2:2631735/100‑1 (MQ=255)

                                        gCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTgg                                                      <  1:4668702/100‑1 (MQ=255)

                                         cGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGt                                                     <  1:2717205/100‑1 (MQ=255)

                                          ggTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTc                                                    >  1:774701/1‑100 (MQ=255)

                                            tGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCaa                                                  >  1:5111601/1‑100 (MQ=255)

                                             ggCAATCAATTACCTGTTCCAGCCTCCTCCGCACTGGGGAAACGATGTTGTCCTCCGTAACACGCTGGTTTCATCTGCGATGATCGACCATGCGGTccag                                                 <  2:1007216/100‑4 (MQ=255)

                                             ggCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAAc                                                 >  1:4342406/1‑100 (MQ=255)

                                               caatcaatTACCTGTTCCAGCATTGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGa                                               >  1:1120658/1‑100 (MQ=255)

                                               caatcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAACGATGTTGTCGTCGGTAAAATGCCGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGa                                               >  2:2759343/1‑100 (MQ=255)

                                               caatcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGa                                               >  2:29622/1‑100 (MQ=255)

                                               caatcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGa                                               >  2:2243648/1‑100 (MQ=255)

                                                aatcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGAc                                              >  2:1644128/1‑100 (MQ=255)

                                                 atcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACt                                             >  1:1559321/1‑100 (MQ=255)

                                                 atcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACt                                             <  1:1056484/100‑1 (MQ=255)

                                                 atcaatTACCTGTTCCAGCATCGTCCGCAGTGCGGCAAAGCTGTTGTCGTCGCTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACt                                             >  2:4989285/1‑100 (MQ=255)

                                                   caatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTg                                           <  1:2500018/100‑1 (MQ=255)

                                                    aatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTgg                                          >  1:1463658/1‑100 (MQ=255)

                                                      ttACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGc                                        <  2:2529218/100‑1 (MQ=255)

                                                      ttACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGc                                        <  1:972055/100‑1 (MQ=255)

                                                        aCCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCg                                      <  1:2073251/100‑1 (MQ=255)

                                                         ccTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTGATCTTCGATGATCGACCGTGTGGTCAACGACTTGGGCCGt                                     <  2:4956025/100‑1 (MQ=255)

                                                          cTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTa                                    >  1:4929178/1‑100 (MQ=255)

                                                           tGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTaa                                   >  2:3928754/1‑100 (MQ=255)

                                                            gTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTaaa                                  >  1:1112429/1‑100 (MQ=255)

                                                              tCCAGGATCGTCCGCAGTGGGGGAAAGATGTTGTCGTCGGTAAAACGCGGGTTTCATCTGCTATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACt                                <  2:2079115/100‑1 (MQ=255)

                                                              nccAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACt                                >  2:4663889/2‑100 (MQ=255)

                                                               ccAGCATCGTCCGCAGTGGGGCAAGGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTg                               >  2:3346703/1‑100 (MQ=255)

                                                               ccAGCATCGTCCGCAGTGGGCCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTg                               >  1:4033774/1‑100 (MQ=255)

                                                                cAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTgg                              >  2:2402383/1‑100 (MQ=255)

                                                                cAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTgg                              >  2:2078636/1‑100 (MQ=255)

                                                                cAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTgg                              >  2:4416243/1‑100 (MQ=255)

                                                                cAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTgg                              <  1:1803204/100‑1 (MQ=255)

                                                                 aGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGt                             <  1:1016262/100‑1 (MQ=255)

                                                                      cGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAg                        <  1:2740634/100‑1 (MQ=255)

                                                                      cGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAg                        >  2:2836118/1‑100 (MQ=255)

                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAGACTGGTAGAAGt                       >  2:4839347/1‑100 (MQ=255)

                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGt                       >  1:1246118/1‑100 (MQ=255)

                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGt                       >  1:410305/1‑100 (MQ=255)

                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGt                       >  2:1307944/1‑100 (MQ=255)

                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGt                       >  2:388063/1‑100 (MQ=255)

                                                                         ccGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTcc                     >  2:4563514/1‑100 (MQ=255)

                                                                         ccGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTcc                     >  1:102945/1‑100 (MQ=255)

                                                                          cGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTccc                    <  2:1990935/100‑1 (MQ=255)

                                                                          cGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTccc                    >  1:4602307/1‑100 (MQ=255)

                                                                              gTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTa                <  1:4350948/100‑1 (MQ=255)

                                                                              gTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTa                >  1:2814749/1‑100 (MQ=255)

                                                                                ggggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAgg              >  2:1570490/1‑100 (MQ=255)

                                                                                ggggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAgg              >  1:4811877/1‑100 (MQ=255)

                                                                                ggggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAgg              <  1:3657434/100‑1 (MQ=255)

                                                                                 gggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGt             >  1:4272086/1‑100 (MQ=255)

                                                                                  ggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGtt            >  2:3752858/1‑100 (MQ=255)

                                                                                  ggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGtt            >  2:1409999/1‑100 (MQ=255)

                                                                                  ggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGtt            >  1:32647/1‑100 (MQ=255)

                                                                                       aGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCaaa       >  1:2641175/1‑100 (MQ=255)

                                                                                         aTGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATg     <  1:2667098/100‑1 (MQ=255)

                                                                                          tgttgtCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATgg    <  2:4258168/100‑1 (MQ=255)

                                                                                            ttGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGtt  >  2:86889/1‑100 (MQ=255)

CGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTT  >  NC_000913/714493‑714684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`