Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 723,678 A→G V246A (GTG→GCG)  kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913723,6780AG98.9% 609.6 / ‑2.9 177V246A (GTG→GCG) kdpDfused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (82/93);  total (84/93)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 180 total aligned reads displayed.

ACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGAT  >  NC_000913/723581‑723774
                                                                                                 |                                                                                                
 cccAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCtt                                                                                               >  2:4880465/1‑100 (MQ=255)
  ccAGCCGTTACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCttt                                                                                              <  2:3394674/100‑1 (MQ=255)
   cAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTtc                                                                                             >  2:2976393/1‑100 (MQ=255)
   cAGCCGTGACGCCAGCCGCGCCGCGGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTtc                                                                                             <  2:1556578/100‑1 (MQ=255)
   cAGCCGGGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTtc                                                                                             <  2:3408465/100‑1 (MQ=255)
   cAGCCGGGACGCCAGCCGCGCCCCTGCGCAGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTtc                                                                                             <  1:4699041/100‑1 (MQ=255)
     gCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCTGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTtctc                                                                                           <  1:4395282/100‑1 (MQ=255)
     gCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTtctc                                                                                           >  2:2013296/1‑100 (MQ=255)
     gCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTtctc                                                                                           <  2:3224167/100‑1 (MQ=255)
     gCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTtctc                                                                                           >  2:3372308/1‑100 (MQ=255)
     gCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTtctc                                                                                           >  1:882334/1‑100 (MQ=255)
      ccGGGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTctct                                                                                          <  1:1937384/100‑1 (MQ=255)
        gTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTc                                                                                        <  1:3373247/100‑1 (MQ=255)
         tGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCg                                                                                       <  2:1418786/100‑1 (MQ=255)
         tGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCg                                                                                       <  2:1633347/100‑1 (MQ=255)
         tGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCg                                                                                       <  1:4873247/100‑1 (MQ=255)
         tGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCg                                                                                       <  1:4215810/100‑1 (MQ=255)
         tGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCg                                                                                       <  2:4452876/100‑1 (MQ=255)
         tGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCg                                                                                       <  2:3201381/100‑1 (MQ=255)
            cGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCa                                                                                    >  2:1223552/1‑100 (MQ=255)
              ccAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATTCATAAAAGGATCGCGTCGCGCATGTGCCACGCTTTCTCTTCGCCAgg                                                                                  <  1:4609136/100‑1 (MQ=255)
               cAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGa                                                                                 >  2:2852241/1‑100 (MQ=255)
               cAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGa                                                                                 >  1:576335/1‑100 (MQ=255)
                aGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGAt                                                                                <  1:1719824/100‑1 (MQ=255)
                 gccgcgccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATg                                                                               >  2:2771820/1‑100 (MQ=255)
                 gccgcgccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATg                                                                               <  2:2712579/100‑1 (MQ=255)
                 gccgcgccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATg                                                                               <  1:1364191/100‑1 (MQ=255)
                 gatctgccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATg                                                                               <  1:3710965/95‑1 (MQ=255)
                  ccgcgccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGc                                                                              <  2:461857/100‑1 (MQ=255)
                   cgcgccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGcc                                                                             >  2:2610985/1‑100 (MQ=255)
                      gccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGccccc                                                                          >  1:738341/1‑100 (MQ=255)
                      gccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGccccc                                                                          <  2:3128915/100‑1 (MQ=255)
                       ccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCg                                                                         <  1:2038541/100‑1 (MQ=255)
                       ccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCg                                                                         <  2:4512388/100‑1 (MQ=255)
                       ccgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCg                                                                         >  2:2121925/1‑100 (MQ=255)
                        cgcTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGc                                                                        >  1:2654189/1‑100 (MQ=255)
                           tGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAg                                                                     <  2:1854212/100‑1 (MQ=255)
                            gcgcGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAgg                                                                    >  2:3832491/1‑100 (MQ=255)
                            gcgcGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCAGAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAgg                                                                    <  1:4327479/100‑1 (MQ=255)
                              tcgGACCAGTTTTTCGCTGCCGGGGTTATGGCCGATGCTTAAAATGATCTCGTCGCGCGTGTTCCACGCTTTCGCTTCGCCAGGAGGCGCCCGCCAggtg                                                                  >  2:231782/2‑98 (MQ=255)
                               cgGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGgcgc                                                                 <  2:3854751/100‑1 (MQ=255)
                                   ccAGTTTTTCGCTGCCGGTGTTATTGCCAATGCATAAAAGGACCGCCTCCCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCAt                                                             <  2:26525/100‑1 (MQ=255)
                                      gTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTg                                                          <  1:2430257/100‑1 (MQ=255)
                                        ttttCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCt                                                        <  1:16003/100‑1 (MQ=255)
                                        ttttCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCt                                                        >  2:2827425/1‑100 (MQ=255)
                                        ttttCGCTGCCGGTGTTATAGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCCGGCGCGCATTTGCt                                                        >  2:4638881/1‑100 (MQ=255)
                                         tttCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCtc                                                       >  2:637418/1‑100 (MQ=255)
                                          ttCGCTGCCGGTGTTATGGCCGCTGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCtca                                                      <  2:119618/100‑1 (MQ=255)
                                          ttCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGACGCCCCCGCCAGGCGCGCATTTGCtca                                                      >  1:4613716/1‑100 (MQ=255)
                                            cGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCtcatc                                                    >  1:3294075/1‑100 (MQ=255)
                                              cttctggTTTTATTGCCGATTCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCaa                                                  >  2:4081318/6‑100 (MQ=255)
                                               tGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAAc                                                 >  1:888626/1‑100 (MQ=255)
                                                gCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAAcg                                                >  2:1497614/1‑100 (MQ=255)
                                                  cGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAAcgcg                                              >  2:2166305/1‑100 (MQ=255)
                                                  cGGTGTTATGGCCGATGCATAAAAGGATCGAGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAAcgcg                                              >  2:2482064/1‑100 (MQ=255)
                                                    gtgtTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGAt                                            >  1:2884524/1‑100 (MQ=255)
                                                      gtTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCg                                          >  2:2667566/1‑100 (MQ=255)
                                                      gtTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCg                                          >  1:3793924/1‑100 (MQ=255)
                                                      gtTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCg                                          <  2:2406697/100‑1 (MQ=255)
                                                      gtTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCg                                          <  1:2226102/100‑1 (MQ=255)
                                                      gtTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCg                                          <  1:856978/100‑1 (MQ=255)
                                                       ttATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCgn                                         <  1:4167136/100‑2 (MQ=255)
                                                        tATGGCCTATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGTCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGc                                        <  2:4722461/100‑1 (MQ=255)
                                                        tATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGc                                        >  1:3198786/1‑100 (MQ=255)
                                                        tATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGc                                        <  1:3092070/100‑1 (MQ=255)
                                                        tATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGc                                        >  2:4143861/1‑100 (MQ=255)
                                                        tATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGc                                        <  1:129056/100‑1 (MQ=255)
                                                        tATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGc                                        >  1:5081433/1‑100 (MQ=255)
                                                         aTGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCa                                       <  1:165210/100‑1 (MQ=255)
                                                         aTGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCa                                       <  2:4350655/100‑1 (MQ=255)
                                                         aTGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCa                                       <  1:3002093/100‑1 (MQ=255)
                                                          tGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAg                                      <  1:3944723/100‑1 (MQ=255)
                                                           ggCCGATGCATAAAAGGATCGCGTCTCGCGTGTGCCACGCTTTCTCTTCACCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGt                                     >  2:2195719/1‑100 (MQ=255)
                                                           ggCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGt                                     >  2:4560358/1‑100 (MQ=255)
                                                           ggCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGt                                     >  1:628489/1‑100 (MQ=255)
                                                            gCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTa                                    >  2:1154794/1‑100 (MQ=255)
                                                            gCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTa                                    >  2:335077/1‑100 (MQ=255)
                                                                aTGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGgc                                <  1:3041469/100‑1 (MQ=255)
                                                                aTGCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGgc                                <  2:3265832/100‑1 (MQ=255)
                                                                  gCATAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGgcgc                              >  1:457027/1‑100 (MQ=255)
                                                                    aTAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAg                            >  2:1678251/1‑100 (MQ=255)
                                                                     tAAAAGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGt                           >  2:2890665/1‑100 (MQ=255)
                                                                        aaGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGcc                        <  2:4256770/100‑1 (MQ=255)
                                                                        aaGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGcc                        <  1:1346024/100‑1 (MQ=255)
                                                                        aaGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGcc                        <  1:3511280/100‑1 (MQ=255)
                                                                         aGGATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCa                       >  1:2690234/1‑100 (MQ=255)
                                                                          ggATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAg                      >  1:1741767/1‑100 (MQ=255)
                                                                           gATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGt                     >  2:257906/1‑100 (MQ=255)
                                                                           gATCGCGTCGCGCGTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGt                     >  2:220205/1‑100 (MQ=255)
                                                                                   cgcgcgTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAg             <  1:688364/100‑1 (MQ=255)
                                                                                    gcgcgTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAgg            <  1:2528598/100‑1 (MQ=255)
                                                                                    gcgcgTGTGCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAgg            >  1:37643/1‑100 (MQ=255)
                                                                                          gtgCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATc      >  2:3105653/1‑100 (MQ=255)
                                                                                           tgCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCa     >  1:1622943/1‑100 (MQ=255)
                                                                                            gCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCCGTTGCCCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAg    >  1:2732453/1‑100 (MQ=255)
                                                                                            gCCACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAg    >  2:3634571/1‑100 (MQ=255)
                                                                                             ccACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGa   >  2:4214540/1‑100 (MQ=255)
                                                                                             ccACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGa   <  1:4752104/100‑1 (MQ=255)
                                                                                             ccACGCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGa   <  2:3738759/100‑1 (MQ=255)
                                                                                              cacgCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGAt  >  1:1191223/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGAT  >  NC_000913/723581‑723774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: