Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,932,950 T→C E552G (GAA→GGA)  edd ← 6‑phosphogluconate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,932,9500TC98.1% 546.1 / ‑3.1 158E552G (GAA→GGA) edd6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (77/78);  total (78/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

ATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGAC  >  NC_000913/1932855‑1933045
                                                                                               |                                                                                               
ataatTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGcc                                                                                             <  1:2666683/100‑1 (MQ=255)
  aatTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctg                                                                                           <  2:1590659/100‑1 (MQ=255)
  aatTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctg                                                                                           <  2:2967012/100‑1 (MQ=255)
  aatTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctg                                                                                           <  1:3423140/100‑1 (MQ=255)
   atTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctgt                                                                                          >  2:4982992/1‑100 (MQ=255)
    ttCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctgtc                                                                                         <  1:2616539/100‑1 (MQ=255)
    ttCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctgtc                                                                                         <  1:1363545/100‑1 (MQ=255)
    ttCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctgtc                                                                                         <  2:2510759/100‑1 (MQ=255)
    ttCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctgtc                                                                                         <  1:2542619/100‑1 (MQ=255)
      cACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctgtctg                                                                                       <  1:362499/100‑1 (MQ=255)
      cACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCctgtctg                                                                                       <  1:2161062/100‑1 (MQ=255)
        cGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCtgtctgtc                                                                                     <  1:2605368/100‑1 (MQ=255)
        cGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCtgtctgtc                                                                                     <  2:2303524/100‑1 (MQ=255)
          tCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCa                                                                                   <  1:975734/100‑1 (MQ=255)
              gTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTca                                                                               >  1:3674773/1‑100 (MQ=255)
              gTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTca                                                                               <  1:4487037/100‑1 (MQ=255)
              gTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTca                                                                               <  1:109513/100‑1 (MQ=255)
                tCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTcaca                                                                             >  1:3846810/1‑100 (MQ=255)
                tCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTcaca                                                                             >  2:5033149/1‑100 (MQ=255)
                  ccACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACg                                                                           >  1:2341195/1‑100 (MQ=255)
                   cACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGa                                                                          >  1:211910/1‑100 (MQ=255)
                     cgcgTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAat                                                                        >  1:1504847/1‑100 (MQ=255)
                     cgcgTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAat                                                                        <  1:4481445/100‑1 (MQ=255)
                      gcgTGGCGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCCGTTCCGCTTCGTCTACCAGGAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAatg                                                                       <  2:4116462/100‑1 (MQ=255)
                      gcgTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAatg                                                                       <  2:4796308/100‑1 (MQ=255)
                      gcgTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAatg                                                                       <  2:4945119/100‑1 (MQ=255)
                       cgTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAatga                                                                      >  1:3425611/1‑100 (MQ=255)
                       cgTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAatga                                                                      >  2:2256932/1‑100 (MQ=255)
                         tGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAatgatg                                                                    >  2:419331/1‑100 (MQ=255)
                          gACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGt                                                                   >  1:2893812/1‑100 (MQ=255)
                          gACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACAAATGATGt                                                                   >  1:4916576/1‑100 (MQ=255)
                            cgcgCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTcc                                                                 >  1:3944324/1‑100 (MQ=255)
                            cgcgCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTcc                                                                 >  2:2555300/1‑100 (MQ=255)
                             gcgcTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTccc                                                                <  1:5018296/100‑1 (MQ=255)
                               gcTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCg                                                              >  2:3695480/1‑100 (MQ=255)
                                    ggtcaggAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGCATGATGTCCCCGTcgcg                                                         >  1:1260193/1‑100 (MQ=255)
                                    ggtcaggAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTcgcg                                                         >  1:4963896/1‑100 (MQ=255)
                                    ggtcaggAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTcgcg                                                         >  1:1258691/1‑100 (MQ=255)
                                    ggtcaggAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTcgcg                                                         >  2:3827072/1‑100 (MQ=255)
                                    ggtcaggAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTcgcg                                                         >  2:70749/1‑100 (MQ=255)
                                     gtcaggAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCgcgc                                                        <  2:137723/100‑1 (MQ=255)
                                     gtcaggAATGTGCGGTTCGCGAGCAGCCAGTTCCACTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCgcgc                                                        >  1:2856631/1‑100 (MQ=255)
                                      tcaggAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCa                                                       <  2:4886052/100‑1 (MQ=255)
                                       caggAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCAc                                                      >  1:3263490/1‑100 (MQ=255)
                                        aggAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACt                                                     <  2:4062938/100‑1 (MQ=255)
                                         ngAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACtt                                                    >  1:4086982/2‑100 (MQ=255)
                                            aTGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTg                                                 <  1:1026816/100‑1 (MQ=255)
                                            aTGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTg                                                 <  1:2623839/100‑1 (MQ=255)
                                            aTGTGCGGTTCGCGAGCAGCCAGTGCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTg                                                 <  2:839939/100‑1 (MQ=255)
                                             tgtgCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGc                                                <  2:2463349/100‑1 (MQ=255)
                                               tgCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCca                                              >  2:317069/1‑100 (MQ=255)
                                                  ggTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCcagca                                           <  1:885153/100‑1 (MQ=255)
                                                  ggTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCcagca                                           <  1:4675626/100‑1 (MQ=255)
                                                   gTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCcagcag                                          >  2:1419048/1‑100 (MQ=255)
                                                    ttCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCagcagc                                         >  1:4080775/1‑100 (MQ=255)
                                                        cgAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGc                                     >  2:3984903/1‑100 (MQ=255)
                                                          agcagcCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCa                                   <  1:4006436/100‑1 (MQ=255)
                                                           gccgcCAGTTCCGCTTCCCATCCCGGCAGCGTCAGCCCCCCTGCCTGCCCATTCACACGAACGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCAt                                  <  2:2329582/97‑1 (MQ=255)
                                                            cagcCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATc                                 >  1:66532/1‑100 (MQ=255)
                                                             tgcCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCg                                >  1:4730961/2‑100 (MQ=255)
                                                              gcCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGt                               >  1:3171878/1‑100 (MQ=255)
                                                              gcCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGt                               >  1:3171903/1‑100 (MQ=255)
                                                               ccAGTTCTGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTa                              <  2:4715807/100‑1 (MQ=255)
                                                                 ggTTCCTCGTCGTGTCCCAGCAGCGTCAGTTCGTCTGTCTGTCCATTCACACGGATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCCTAgg                            <  2:4285967/99‑1 (MQ=255)
                                                                  gTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGc                           <  1:3070638/100‑1 (MQ=255)
                                                                  gTTCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGc                           <  1:3070657/100‑1 (MQ=255)
                                                                   ttCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCt                          <  1:4321322/100‑1 (MQ=255)
                                                                   ctCCGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCt                          <  1:4978932/99‑1 (MQ=255)
                                                                     ccGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTc                        <  1:5042371/100‑1 (MQ=255)
                                                                      cGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCt                       >  1:4195968/1‑100 (MQ=255)
                                                                      cGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCt                       <  2:101350/100‑1 (MQ=255)
                                                                      cGCTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCt                       >  2:2836508/1‑100 (MQ=255)
                                                                        cTTCGTCTACCAGCATCGTCAGTCCGCTTGTCTGCCCATTTACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCTGCCCGCCATCGTAGGCTTCTgg                     <  2:4855377/100‑1 (MQ=255)
                                                                        cTTCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTgg                     >  2:1585612/1‑100 (MQ=255)
                                                                         ttCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGgt                    >  2:1723649/1‑100 (MQ=255)
                                                                         ttCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGgt                    <  1:1774366/100‑1 (MQ=255)
                                                                          tCGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGgtg                   <  2:1528672/100‑1 (MQ=255)
                                                                           cGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGgtgt                  <  2:2056460/100‑1 (MQ=255)
                                                                           cGTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCACGCGCCCGCCATCGTAGGCTTCTGgtgt                  >  2:2624394/1‑100 (MQ=255)
                                                                            gTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGtt                 <  2:469977/100‑1 (MQ=255)
                                                                            gTCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGtt                 >  2:2624950/1‑100 (MQ=255)
                                                                            gTCAACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGtt                 >  2:4666533/1‑100 (MQ=255)
                                                                             tCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTa                <  2:2582146/100‑1 (MQ=255)
                                                                             tCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTa                >  1:926352/1‑100 (MQ=255)
                                                                             tCTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTa                <  1:1721559/100‑1 (MQ=255)
                                                                              cTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTAc               >  2:3297359/1‑100 (MQ=255)
                                                                              cTACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTAc               >  1:1546099/1‑100 (MQ=255)
                                                                               tACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACg              <  2:661561/100‑1 (MQ=255)
                                                                               tACCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACg              <  1:4011148/100‑1 (MQ=255)
                                                                                aCCAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGt             <  1:4308313/100‑1 (MQ=255)
                                                                                 ccAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTg            <  1:1769971/100‑1 (MQ=255)
                                                                                 ccAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTg            <  2:3233447/100‑1 (MQ=255)
                                                                                 ccAGCAGCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTg            >  2:4483749/1‑100 (MQ=255)
                                                                                  cagcagCGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTgg           <  2:1698885/100‑1 (MQ=255)
                                                                                   agcagcGTCAGTCCGCCCGACAGACCATTCACACGCCGGCTGTCCCCCTCGGGCTCTTTAGCTAGCAGCCGTCGACCGTACGTTTCTGGTGTTACGTGGa          >  2:4781345/1‑100 (MQ=255)
                                                                                    gcagcGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGAt         <  2:3701210/100‑1 (MQ=255)
                                                                                       gcGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGc      <  2:439454/100‑1 (MQ=255)
                                                                                        cGTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCt     >  1:1431981/1‑100 (MQ=255)
                                                                                         gTCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTg    <  2:4544390/100‑1 (MQ=255)
                                                                                          tCAGTCCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGa   >  1:3853052/1‑100 (MQ=255)
                                                                                               |                                                                                               
ATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGAC  >  NC_000913/1932855‑1933045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: