Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 101,342 C→T T193I (ACC→ATC)  murC → UDP‑N‑acetylmuramate:L‑alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913101,3420CT99.4% 523.1 / ‑4.8 154T193I (ACC→ATC) murCUDP‑N‑acetylmuramate:L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (79/74);  total (79/75)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 158 total aligned reads displayed.

GTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGG  >  NC_000913/101245‑101438
                                                                                                 |                                                                                                
gTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGtcatca                                                                                                <  1:102793/100‑1 (MQ=255)
 ttCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCaa                                                                                               <  1:4207638/100‑1 (MQ=255)
  tCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAat                                                                                              <  2:3115366/100‑1 (MQ=255)
     tgagcgTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGAGGAGAGTGATGCATCGTTCCTGCATCTGCAACTGATGTTGGCGATTGTCATCAATATc                                                                                           <  2:2781045/97‑1 (MQ=255)
       cgcgTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGa                                                                                         <  2:3041454/100‑1 (MQ=255)
         cgTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAg                                                                                       >  1:1277106/1‑100 (MQ=255)
            ttGGGGCATGGTTGGTACCTGATTGCCGAACCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCg                                                                                    <  2:1599130/100‑1 (MQ=255)
            ttGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCg                                                                                    <  2:588677/100‑1 (MQ=255)
             tGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGa                                                                                   >  2:872705/1‑100 (MQ=255)
             tGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGa                                                                                   >  2:4274449/1‑100 (MQ=255)
              ggggCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGAc                                                                                  <  2:4986019/100‑1 (MQ=255)
              ggggCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGAc                                                                                  >  1:3137771/1‑100 (MQ=255)
                ggCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACca                                                                                >  2:3898101/1‑100 (MQ=255)
                ggCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACca                                                                                <  1:2865523/100‑1 (MQ=255)
                 gCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACcac                                                                               >  1:166192/1‑100 (MQ=255)
                    tGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATg                                                                            >  2:3415544/1‑100 (MQ=255)
                     ggTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATgg                                                                           >  1:1419877/1‑100 (MQ=255)
                     ggTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATgg                                                                           <  2:264610/100‑1 (MQ=255)
                       tCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGAt                                                                         >  1:941118/1‑100 (MQ=255)
                             ccTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAATCCGACCACATGGAtacctac                                                                   >  2:4454678/1‑100 (MQ=255)
                              cTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGAtacctacc                                                                  >  2:1620422/1‑100 (MQ=255)
                              cTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGAtacctacc                                                                  <  1:2524606/100‑1 (MQ=255)
                              cTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGAtacctacc                                                                  <  1:5138308/100‑1 (MQ=255)
                              cTGATTGCCGAAGAAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGAtacctacc                                                                  <  2:1591346/100‑1 (MQ=255)
                               tGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCa                                                                 >  2:2380260/1‑100 (MQ=255)
                               tGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCa                                                                 >  1:636946/1‑100 (MQ=255)
                                   tGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATTGATACCTACCAGGGc                                                             >  2:3854308/1‑100 (MQ=255)
                                   tGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGc                                                             <  2:3253759/100‑1 (MQ=255)
                                    gCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGCTTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCg                                                            >  2:469983/1‑100 (MQ=255)
                                    gCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCg                                                            >  1:2991570/1‑100 (MQ=255)
                                    gCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCg                                                            >  1:4247110/1‑100 (MQ=255)
                                     ccGAAGCAGATGGGGGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGa                                                           <  1:2597506/100‑1 (MQ=255)
                                      cGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGTTACCTACCAGGGCGAc                                                          >  2:614308/1‑100 (MQ=255)
                                      cGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGAc                                                          <  2:3829603/100‑1 (MQ=255)
                                       gAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACt                                                         >  1:1397621/1‑100 (MQ=255)
                                       gAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACt                                                         >  2:3254159/1‑100 (MQ=255)
                                       gAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACt                                                         >  2:2269468/1‑100 (MQ=255)
                                          gCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTg                                                      <  1:4190259/100‑1 (MQ=255)
                                          gCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTg                                                      <  1:4337490/100‑1 (MQ=255)
                                           cAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTga                                                     <  2:2973162/100‑1 (MQ=255)
                                             gATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTgaga                                                   >  1:1956758/1‑100 (MQ=255)
                                             gATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTgaga                                                   <  1:3453623/100‑1 (MQ=255)
                                              tctagagTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGaa                                                  <  2:1457136/97‑1 (MQ=255)
                                              aTGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGaa                                                  <  2:1206044/100‑1 (MQ=255)
                                               tGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAAt                                                 <  1:3941134/100‑1 (MQ=255)
                                                gagaGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAAtt                                                <  2:2815167/100‑1 (MQ=255)
                                                gagaGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAAtt                                                <  2:1083889/100‑1 (MQ=255)
                                                gagaGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAAtn                                                <  1:4088023/100‑2 (MQ=255)
                                                gagaGTGATGCATCGTTCCTGCATCTGCAACCGAAGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTCGAGAAtt                                                >  2:4121624/1‑100 (MQ=255)
                                                 agagTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGaaaga                                               >  1:1478580/1‑97 (MQ=255)
                                                 agagTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAAttt                                               <  2:2806218/100‑1 (MQ=255)
                                                 agagTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAAttt                                               >  1:4713882/1‑100 (MQ=255)
                                                  gagTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTa                                              >  1:2194988/1‑100 (MQ=255)
                                                  gagTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTa                                              >  1:2192227/1‑100 (MQ=255)
                                                   ggTGATGCATCGTGCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTaa                                             <  1:704084/99‑1 (MQ=255)
                                                   agTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACGTTGAGAATTTaa                                             <  1:4444704/100‑1 (MQ=255)
                                                    gTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTaaa                                            <  1:553844/100‑1 (MQ=255)
                                                     tGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTaaaa                                           <  1:3933604/100‑1 (MQ=255)
                                                      gATGAATCGTTCCTGCATCTGAAACCGATCGTCGCGATTGTCATCAATCTCGAAGCCCACCACATGGATACCTACCAGGGCGACTTTGAGACTTTACAAc                                          <  2:749074/100‑1 (MQ=255)
                                                        tGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACCTGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAg                                        >  1:4930203/1‑100 (MQ=255)
                                                        tGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAg                                        <  1:1306867/100‑1 (MQ=255)
                                                        tGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAg                                        >  2:1741102/1‑100 (MQ=255)
                                                        tGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAg                                        >  1:140729/1‑100 (MQ=255)
                                                          cATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATATCTACCAGGGCGACTTTGAGAATTTAAAACAGAc                                      <  2:781200/100‑1 (MQ=255)
                                                          cATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGAc                                      >  1:1140959/1‑100 (MQ=255)
                                                          cATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGAc                                      >  1:3461594/1‑100 (MQ=255)
                                                          cATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGAc                                      >  1:1207991/1‑100 (MQ=255)
                                                          cATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGAc                                      <  1:341008/100‑1 (MQ=255)
                                                           aTCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACt                                     <  2:3297301/100‑1 (MQ=255)
                                                             cGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACttt                                   <  1:3290686/100‑1 (MQ=255)
                                                               ttCCTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTtta                                 <  1:4214632/100‑1 (MQ=255)
                                                                 ccTGCGTCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTttatn                               <  1:1640365/100‑2 (MQ=255)
                                                                 ccTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTttatt                               >  1:2682424/1‑100 (MQ=255)
                                                                 ccTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTttatt                               >  2:4823316/1‑100 (MQ=255)
                                                                  cTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTttatta                              >  1:3556294/1‑100 (MQ=255)
                                                                  cTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTttatta                              <  2:3485718/100‑1 (MQ=255)
                                                                  cTGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTttatta                              >  2:1536525/1‑100 (MQ=255)
                                                                   tGCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTaa                             >  1:2838768/1‑100 (MQ=255)
                                                                    gCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGAGCACATGTATACCTACCAGGGCGACTTTTAGAATTTAAAAGAGACTTTTATTAAt                            <  2:4752906/100‑1 (MQ=255)
                                                                    gCATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAAt                            >  1:4037101/1‑100 (MQ=255)
                                                                     cATCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGCATTTAACACAGACTTTTATTAAtt                           >  2:5012824/1‑100 (MQ=255)
                                                                       tCTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAAtttt                         <  1:3911042/100‑1 (MQ=255)
                                                                        cTGCAACCGATGGTGGCGATTGTCATCAATATCGCAGCCGACCACATGGATACGTAACAGGGCGAATCTTAGAATTTAAAATCGACTTTTATTAAttatt                        >  2:1234073/1‑97 (MQ=255)
                                                                        cTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTc                        <  2:2589661/100‑1 (MQ=255)
                                                                        cTGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTc                        <  2:1136122/100‑1 (MQ=255)
                                                                         tGCAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCt                       >  1:3214928/1‑100 (MQ=255)
                                                                           cAACCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGc                     <  2:2600083/100‑1 (MQ=255)
                                                                            aaCCGATGGTGGCGACTGTCCTCAATATCCAAGCCGATCACACGGATCCCTACCAGCGCGACTTTCAGAATTCACAACAGACTTTCATCACTTATCTGca                    >  2:4853480/1‑100 (MQ=255)
                                                                             aCCGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGcac                   >  2:2778145/1‑100 (MQ=255)
                                                                              ccGATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGcaca                  >  1:3704353/1‑100 (MQ=255)
                                                                                gATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAAc                >  2:2130666/1‑100 (MQ=255)
                                                                                gATGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAAc                >  2:2130620/1‑100 (MQ=255)
                                                                                 aTGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAAcc               >  1:1102794/1‑100 (MQ=255)
                                                                                 aTGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAAcc               <  2:2905013/100‑1 (MQ=255)
                                                                                 aTGGTGGCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAAcc               <  2:2456993/100‑1 (MQ=255)
                                                                                    gtggCGATTGTCATCAATATCGAAGCCGACCACATGGATACCTCCCAGGGCGACTTTGAGTATTTAAAACAGACTTTTATTAATCTTCTGCACAACCTGc            >  2:3003411/1‑100 (MQ=255)
                                                                                            tGTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTAc    <  1:1655289/100‑1 (MQ=255)
                                                                                             gTCATCAATATCGAAGCCGACCACATGGTTACCTACCAGGGCGACATTGAGAATTTAAAACACACTTTTATCAATTTTCTGTACCATCTGCAGTTTTCCg   >  2:2034792/1‑100 (MQ=255)
                                                                                             gTCATCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACg   <  1:4171438/100‑1 (MQ=255)
                                                                                             gTCATCAATATCGAAGCCGACCACATAGAGACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACg   <  1:4171076/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCATCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCACCAATATCGAAGCCGACCACATGGATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGG  >  NC_000913/101245‑101438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑