Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,267,405 A→G I387M (ATA→ATG yeiQ → putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,267,4050AG100.0% 732.9 / NA 203I387M (ATA→ATGyeiQputative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (100/103);  total (100/103)

Only 100 of 205 total aligned reads displayed.

CACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATT  >  NC_000913/2267308‑2267501
                                                                                                 |                                                                                                
cACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAGTTACCGCAACGCATGCTGGCAGGTATTCGCATGCa                                                                                                >  2:5007481/1‑100 (MQ=255)
  cGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATc                                                                                              <  1:3145752/100‑1 (MQ=255)
    ttttGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAg                                                                                            >  1:2429346/1‑100 (MQ=255)
     tttGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAgg                                                                                           <  2:3460726/100‑1 (MQ=255)
        gCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGgc                                                                                        >  1:1462207/1‑100 (MQ=255)
         cTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGgcg                                                                                       >  2:1234618/1‑100 (MQ=255)
         cTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGgcg                                                                                       >  1:3454898/1‑100 (MQ=255)
         cTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGgcg                                                                                       >  2:1334208/1‑100 (MQ=255)
          tAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGgcgc                                                                                      <  2:2858871/100‑1 (MQ=255)
           aaTCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGcgcg                                                                                     >  2:2278574/1‑100 (MQ=255)
           aaTCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGcgcg                                                                                     >  1:947381/1‑100 (MQ=255)
           aaTCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGcgcg                                                                                     <  2:88660/100‑1 (MQ=255)
            aTCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGa                                                                                    >  1:1682660/1‑100 (MQ=255)
              ccGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGaaa                                                                                  >  2:1414201/1‑100 (MQ=255)
              ccGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGaaa                                                                                  >  1:49334/1‑100 (MQ=255)
              ccGGCGCTGAAACATAAGACCCGGCAAATCGCGATGGTTGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGaaa                                                                                  <  1:4801352/100‑1 (MQ=255)
                ggCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACg                                                                                <  2:1592171/100‑1 (MQ=255)
                 gcgcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACgg                                                                               <  2:3038552/100‑1 (MQ=255)
                 gcgcTGAAACATAAGACCTGGAAAATCGGGATGGATGGCAGCCAGAAATGACCGCAACGCACGCTGGGAGGGAGTCGCATGCATCAGGGGCGCGAAACgg                                                                               <  2:3302528/100‑1 (MQ=255)
                 gcgcTGAAACATAAGACCTCGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCACGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACgg                                                                               <  1:4582726/100‑1 (MQ=255)
                  cgcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGa                                                                              <  1:3068828/100‑1 (MQ=255)
                   gcTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGAc                                                                             <  1:2264715/100‑1 (MQ=255)
                      ggaaCATAAGACCTGGCAATTCGCGATGGATGGCAGCCAGAAATTACCGCAACTCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACAgg                                                                          >  2:962597/3‑100 (MQ=255)
                      gAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTgg                                                                          >  1:3323620/1‑100 (MQ=255)
                      gAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTgg                                                                          <  1:3701273/100‑1 (MQ=255)
                       aaaCATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGt                                                                         <  1:2077993/100‑1 (MQ=255)
                       aaaCATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGt                                                                         <  1:2305925/100‑1 (MQ=255)
                        aaCATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGta                                                                        >  1:3860784/1‑99 (MQ=255)
                        aaCATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTc                                                                        <  1:1652577/100‑1 (MQ=255)
                         aCATAAGACCTGGCAACTCGCGATGGATAGCAGCCAGCAATTACCGCAACGCATCCTGGCAGGTATTCGCATGCATCCGGGGCGCGAAACGGACTGGTCg                                                                       >  2:744685/1‑100 (MQ=255)
                          cATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGt                                                                      >  1:3814115/1‑100 (MQ=255)
                            tAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTg                                                                    >  1:3524876/1‑100 (MQ=255)
                            tAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTg                                                                    <  2:2602640/100‑1 (MQ=255)
                             aaGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGc                                                                   >  2:1085370/1‑100 (MQ=255)
                             aaGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGc                                                                   <  1:1899349/100‑1 (MQ=255)
                             aaGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCAGGCATCAGGGGCGCGAAACGGACTGGTCGTTGc                                                                   <  2:1709652/100‑1 (MQ=255)
                                aCCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTgg                                                                <  1:2320575/100‑1 (MQ=255)
                                 ccTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGc                                                               >  2:4618104/1‑100 (MQ=255)
                                  cTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCa                                                              <  2:1978293/100‑1 (MQ=255)
                                   tGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCAt                                                             <  1:5059728/100‑1 (MQ=255)
                                    ggCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCAtt                                                            >  2:2900820/1‑100 (MQ=255)
                                    ggCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCAtt                                                            >  2:1511046/1‑100 (MQ=255)
                                      cAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAg                                                          <  1:2785771/100‑1 (MQ=255)
                                      cAAATCGCGAGGGAAGGCAGCCAGAAGTTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGAATTac                                                          >  2:4735793/1‑99 (MQ=255)
                                       aaaTCTCGATTGATAGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGTACTGGTCGTTGCTGGCATTAgg                                                         <  2:3357409/100‑1 (MQ=255)
                                        aaTCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGc                                                        <  2:4613362/100‑1 (MQ=255)
                                          tCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGt                                                      <  1:2058727/100‑1 (MQ=255)
                                          acgcgATGGCTGGCAGCCAGAAGTTACCGCGACTCATGCTGGCAGTTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTTGGCGt                                                      <  2:3176214/99‑1 (MQ=255)
                                            gcgATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTg                                                    >  1:4745526/1‑100 (MQ=255)
                                            gcgATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGGCGTTGCTGGCATGAGGCGTTg                                                    >  2:2791246/1‑100 (MQ=255)
                                            gagctggctggcAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGAGAAACGGAATGGTCGTTGCTGGCATTAGGCGCTg                                                    >  2:831393/5‑100 (MQ=255)
                                             cgATGGATTGCAGCCAGAAATGACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACGGGTCGTTGCTGGCATTAGGCGTTGc                                                   >  2:4828952/1‑100 (MQ=255)
                                             cgATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGc                                                   >  2:3807221/1‑100 (MQ=255)
                                              gatggatgGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCa                                                  <  1:3933772/100‑1 (MQ=255)
                                               atggatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAg                                                 <  1:1194636/100‑1 (MQ=255)
                                                tggatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAgg                                                <  1:2459084/100‑1 (MQ=255)
                                                 ggatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGc                                               <  1:2851138/100‑1 (MQ=255)
                                                 ggatggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGc                                               <  1:65824/100‑1 (MQ=255)
                                                   atggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTg                                             <  2:2201088/100‑1 (MQ=255)
                                                   atggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTg                                             <  2:4850505/100‑1 (MQ=255)
                                                    tggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTgg                                            >  1:4848490/1‑100 (MQ=255)
                                                     ggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGTCAGGTCGTTGCTGGAACTAGGCGTTGCAGGGTGGa                                           >  2:715524/1‑100 (MQ=255)
                                                     ggCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGa                                           <  2:613651/100‑1 (MQ=255)
                                                      gCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGAt                                          <  1:3272806/100‑1 (MQ=255)
                                                       cAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATg                                         <  1:1612060/100‑1 (MQ=255)
                                                         gCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCg                                       >  1:1838180/1‑100 (MQ=255)
                                                          ccAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGt                                      <  2:470036/100‑1 (MQ=255)
                                                          ccAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGt                                      >  1:3586178/1‑100 (MQ=255)
                                                            aGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTa                                    <  2:2451190/100‑1 (MQ=255)
                                                              aaaTTACCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACg                                  <  1:4997930/100‑1 (MQ=255)
                                                                   aCCGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgc                             >  2:241320/1‑100 (MQ=255)
                                                                    ccGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcg                            <  2:4163834/100‑1 (MQ=255)
                                                                    ccGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcg                            <  1:3056668/100‑1 (MQ=255)
                                                                    ccGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcg                            <  1:764894/100‑1 (MQ=255)
                                                                    ccGCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcg                            >  2:2489878/1‑100 (MQ=255)
                                                                      gCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcggc                          >  2:199973/1‑100 (MQ=255)
                                                                      gCAACGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAgcggc                          >  2:4709740/1‑100 (MQ=255)
                                                                        aaCGCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGt                        <  2:1817238/100‑1 (MQ=255)
                                                                           gCATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTtga                     <  2:2920160/100‑1 (MQ=255)
                                                                            cATGCTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTtgan                    <  1:1713825/100‑2 (MQ=255)
                                                                                cTGGCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGatgatg                >  1:2658426/1‑100 (MQ=255)
                                                                                  ggCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGcc              <  1:2672017/100‑1 (MQ=255)
                                                                                   gCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCg             <  1:699922/100‑1 (MQ=255)
                                                                                   gCAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCg             <  1:2275976/100‑1 (MQ=255)
                                                                                   gCAGGTATTCGCATGCATCACGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCg             >  1:4025352/1‑100 (MQ=255)
                                                                                    cAGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCgg            >  2:4391273/1‑100 (MQ=255)
                                                                                     aGGTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGa           >  2:1749366/1‑100 (MQ=255)
                                                                                       gTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGaaa         <  1:4778007/100‑1 (MQ=255)
                                                                                       gTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGaaa         >  1:2902167/1‑100 (MQ=255)
                                                                                       gTATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGaaa         >  1:420299/1‑100 (MQ=255)
                                                                                        tATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGGTGGATGCGTTACGTTTGCGGGGTTGATGATGCCGGAAAt        >  2:2642515/1‑100 (MQ=255)
                                                                                        tATTCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAAt        >  1:441005/1‑100 (MQ=255)
                                                                                          ttCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGc      >  2:203024/1‑100 (MQ=255)
                                                                                          ttCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGc      <  2:3224967/100‑1 (MQ=255)
                                                                                          ttCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAACGc      <  1:876600/100‑1 (MQ=255)
                                                                                           tCGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGcc     <  1:1103012/100‑1 (MQ=255)
                                                                                            cGCATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCa    >  1:4716457/1‑100 (MQ=255)
                                                                                              cATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCAtt  >  1:3097584/1‑100 (MQ=255)
                                                                                              cATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCAtt  >  1:391905/1‑100 (MQ=255)
                                                                                              cATGCATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGAAGATGCCGGAAATGCCAtt  <  2:650422/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CACGTTTTGCTAATCCGGCGCTGAAACATAAGACCTGGCAAATCGCGATGGATGGCAGCCAGAAATTACCGCAACGCATGCTGGCAGGTATTCGCATACATCAGGGGCGCGAAACGGACTGGTCGTTGCTGGCATTAGGCGTTGCAGGCTGGATGCGTTACGTCAGCGGCGTTGATGATGCCGGAAATGCCATT  >  NC_000913/2267308‑2267501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: