Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 145,691 C→T A204V (GCC→GTC)  yadE → putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913145,6910CT99.4% 588.8 / NA 173A204V (GCC→GTC) yadEputative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (93/78);  total (94/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 173 total aligned reads displayed.

TGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGAT  >  NC_000913/145595‑145787
                                                                                                |                                                                                                
 gCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCACACTTCGCCGACCACATCGGTACGCGCTTCCAATAACCAGATGGTCTgg                                                                                              >  1:65324/1‑100 (MQ=255)
  cTAACCTATCACCATATTCTGCGCGACGAAGAAAACCCCCGTTTTCGCCATACTTCGACGACCACAGCGGTACGCGCTTTCAATAACCAGATGGTCTGGc                                                                                             <  2:1006431/100‑1 (MQ=255)
  cTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGc                                                                                             <  1:5084066/100‑1 (MQ=255)
  cTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGc                                                                                             <  1:3683981/100‑1 (MQ=255)
   tAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCt                                                                                            >  1:3995541/1‑100 (MQ=255)
     aCCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGc                                                                                          >  1:3711453/1‑100 (MQ=255)
       cTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGt                                                                                        >  2:2734515/1‑100 (MQ=255)
        tATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTg                                                                                       <  1:1106023/100‑1 (MQ=255)
        tATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTg                                                                                       >  2:3165035/1‑100 (MQ=255)
        tATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTg                                                                                       <  1:2322188/100‑1 (MQ=255)
        gatctccATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTg                                                                                       <  2:4537164/95‑1 (MQ=255)
           cACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACa                                                                                    >  2:4502083/1‑100 (MQ=255)
            aCCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAg                                                                                   >  2:1648384/1‑100 (MQ=255)
              cATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAggg                                                                                 <  2:2105665/100‑1 (MQ=255)
               atatTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAgggg                                                                                <  2:2237781/100‑1 (MQ=255)
                tatTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGa                                                                               <  1:1985449/100‑1 (MQ=255)
                  ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATa                                                                             <  1:3201683/100‑1 (MQ=255)
                  ttCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATa                                                                             <  1:3200796/100‑1 (MQ=255)
                    cTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcg                                                                           >  2:1091523/1‑100 (MQ=255)
                     tGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCCCATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcgc                                                                          <  2:4838036/100‑1 (MQ=255)
                     tGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcgc                                                                          >  1:5115294/1‑100 (MQ=255)
                     tGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcgc                                                                          <  2:4759791/100‑1 (MQ=255)
                     tGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcgc                                                                          <  2:4759562/100‑1 (MQ=255)
                      gcgcGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcgcg                                                                         >  1:1522324/1‑100 (MQ=255)
                      gcgcGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcgcg                                                                         <  1:562580/100‑1 (MQ=255)
                      gcgcGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATAcgcg                                                                         <  2:4242746/100‑1 (MQ=255)
                       cgcgACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGa                                                                        >  2:3780454/1‑100 (MQ=255)
                       cgcgACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGa                                                                        >  2:1858221/1‑100 (MQ=255)
                        gcgACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGac                                                                       <  1:2270069/100‑1 (MQ=255)
                          gacgaAGAAACCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATGACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGacac                                                                     <  1:4236044/100‑1 (MQ=255)
                          gacgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGCGACGGGGGTTACGCGacac                                                                     >  2:4591248/1‑100 (MQ=255)
                           acgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACt                                                                    >  2:2253560/1‑100 (MQ=255)
                            cgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTg                                                                   >  2:1309761/1‑100 (MQ=255)
                              aagaaAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAg                                                                 <  1:1897888/100‑1 (MQ=255)
                              aagaaAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAg                                                                 >  1:84803/1‑100 (MQ=255)
                               agcaaaaACCCGTCTTCGCCATACTTCCACGACCACATAGGTACGCGCTTTCAATAACCAGATGCCCTGGCTGAGTGACAGGGGATACGCGACACTGAGc                                                                >  2:3577025/4‑100 (MQ=255)
                               agaaAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGc                                                                <  1:2298402/100‑1 (MQ=255)
                                 aaaaCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCAt                                                              >  2:3093626/1‑100 (MQ=255)
                                  aaaCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATg                                                             >  1:2540083/1‑100 (MQ=255)
                                   aaCACCCGTTTTCGCCATAGTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATgg                                                            <  2:3857526/100‑1 (MQ=255)
                                   aaCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATgg                                                            <  2:1197908/100‑1 (MQ=255)
                                    acacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGGGCATGGt                                                           <  2:1548812/100‑1 (MQ=255)
                                    acacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGt                                                           <  1:2737238/100‑1 (MQ=255)
                                     cacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTg                                                          >  1:4969551/1‑100 (MQ=255)
                                     cacCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTg                                                          >  2:3765676/1‑100 (MQ=255)
                                      acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGc                                                         <  2:4311798/100‑1 (MQ=255)
                                      acCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGc                                                         <  1:1166172/100‑1 (MQ=255)
                                         cGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAAGAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGc                                                      <  2:4226302/100‑1 (MQ=255)
                                          gTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCt                                                     >  2:1034018/1‑100 (MQ=255)
                                           ttttCGCCATACTTCGACGACCACATCGGTACGCGGTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTg                                                    <  2:5012439/100‑1 (MQ=255)
                                              tCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGaa                                                 <  2:2570059/100‑1 (MQ=255)
                                                gCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAgg                                               >  2:3721103/1‑100 (MQ=255)
                                                gCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAgg                                               >  2:2445813/1‑100 (MQ=255)
                                                 ccATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGc                                              <  2:1232818/100‑1 (MQ=255)
                                                 ccATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGc                                              >  2:2147222/1‑100 (MQ=255)
                                                    tACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTAc                                           <  2:4155727/100‑1 (MQ=255)
                                                     aCTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACg                                          <  1:2497751/100‑1 (MQ=255)
                                                      cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGACGGCTACGt                                         >  1:3231298/1‑100 (MQ=255)
                                                      cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGt                                         <  1:2025643/100‑1 (MQ=255)
                                                      cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGt                                         <  1:2224748/100‑1 (MQ=255)
                                                      cTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGt                                         >  2:4346740/1‑100 (MQ=255)
                                                       ttCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTg                                        <  2:1818562/100‑1 (MQ=255)
                                                          gacgacCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaag                                     <  1:1697807/100‑1 (MQ=255)
                                                            cgacCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaagaa                                   <  1:2858810/100‑1 (MQ=255)
                                                              acCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATa                                 >  2:72392/1‑100 (MQ=255)
                                                              acCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATa                                 >  2:1159584/1‑100 (MQ=255)
                                                              acCACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATa                                 >  1:347552/1‑100 (MQ=255)
                                                               ccACATCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATaa                                >  2:4986455/1‑100 (MQ=255)
                                                                ctcaTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAg                               <  1:3086408/98‑1 (MQ=255)
                                                                 acaTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGa                              >  1:4362567/1‑100 (MQ=255)
                                                                  caTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGCTACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGAt                             >  1:4215273/1‑100 (MQ=255)
                                                                  caTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGCGCATGGTGCAGCTGGAAGGCTAAGTGAGGAAAAAGag                             >  2:3051949/1‑99 (MQ=255)
                                                                  caTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGAt                             <  2:4163051/100‑1 (MQ=255)
                                                                  caTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGAt                             >  1:4955895/1‑100 (MQ=255)
                                                                   aTCGGTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATc                            <  1:3503309/100‑1 (MQ=255)
                                                                      ggTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAt                         >  1:2929418/1‑100 (MQ=255)
                                                                      ggTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAt                         >  2:2849518/1‑100 (MQ=255)
                                                                      ggTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCAGGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAt                         >  2:4614731/1‑100 (MQ=255)
                                                                       gTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAtc                        >  2:3048309/1‑100 (MQ=255)
                                                                       gTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAtc                        <  1:3123452/100‑1 (MQ=255)
                                                                       gTACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAtc                        >  2:453943/1‑100 (MQ=255)
                                                                       gTACGCGCTTTCAATAACCAGATGGGCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAtc                        <  2:1616753/100‑1 (MQ=255)
                                                                        tACGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAtct                       >  2:2113858/1‑100 (MQ=255)
                                                                         aCGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAtctc                      <  2:2510884/100‑1 (MQ=255)
                                                                         aCGCGCTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAAtctc                      >  2:620353/1‑100 (MQ=255)
                                                                           gcgcTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTccc                    <  1:3767884/100‑1 (MQ=255)
                                                                            cgcTTTCAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCt                   >  1:2552768/1‑100 (MQ=255)
                                                                                  cAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGa             >  1:263611/1‑100 (MQ=255)
                                                                                  cAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGa             >  2:1504272/1‑100 (MQ=255)
                                                                                  cAATAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGa             >  2:3984153/1‑100 (MQ=255)
                                                                                   aaTAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAg            >  1:1633622/1‑100 (MQ=255)
                                                                                   aaTAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAg            >  1:1438931/1‑100 (MQ=255)
                                                                                   aaTAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAg            >  2:193841/1‑100 (MQ=255)
                                                                                    aTAACCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGc           >  2:1817938/1‑100 (MQ=255)
                                                                                      aaCCAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCCACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCgg         >  1:4880530/1‑100 (MQ=255)
                                                                                        ccAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggtg       >  2:2712460/1‑100 (MQ=255)
                                                                                         cAGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggtgg      <  1:390479/100‑1 (MQ=255)
                                                                                          aGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGTGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGAGCGAGCggtggt     <  2:4891654/100‑1 (MQ=255)
                                                                                          aGATGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCggtggt     <  1:459783/100‑1 (MQ=255)
                                                                                             tGGTCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGAt  >  2:3556320/1‑100 (MQ=255)
                                                                                                |                                                                                                
TGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGAT  >  NC_000913/145595‑145787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: