Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,653,277 T→C Y262H (TAC→CAC)  sseA → 3‑mercaptopyruvate sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,2770TC98.0% 709.0 / ‑1.2 204Y262H (TAC→CAC) sseA3‑mercaptopyruvate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base C (110/89);  total (113/91)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

CTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTT  >  NC_000913/2653180‑2653372
                                                                                                 |                                                                                               
cTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCac                                                                                               <  2:432534/100‑1 (MQ=255)
   cGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCacgac                                                                                            <  1:3301236/100‑1 (MQ=255)
   cGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCacgac                                                                                            >  2:2556213/1‑100 (MQ=255)
   cGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCacgac                                                                                            <  2:2591826/100‑1 (MQ=255)
    gACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCacgacg                                                                                           >  1:3740065/1‑100 (MQ=255)
     aCAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACgg                                                                                          >  2:1471454/1‑100 (MQ=255)
       aaaCCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGgcg                                                                                        >  1:283327/1‑100 (MQ=255)
       aaaCCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGgcg                                                                                        >  2:3209581/1‑100 (MQ=255)
         aCCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCa                                                                                      >  1:3258644/1‑100 (MQ=255)
         aCCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCa                                                                                      >  2:2474211/1‑100 (MQ=255)
           cAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTGGTTAGCACTAGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATg                                                                                    <  2:4571797/100‑1 (MQ=255)
            aaTTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATgg                                                                                   <  2:2935397/100‑1 (MQ=255)
              ttATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAg                                                                                 >  2:3160731/1‑100 (MQ=255)
               tATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGt                                                                                >  1:1511937/1‑100 (MQ=255)
               tATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGt                                                                                <  1:3913972/100‑1 (MQ=255)
               tATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGt                                                                                <  2:2433983/100‑1 (MQ=255)
                  cGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGaa                                                                             >  1:1084146/1‑100 (MQ=255)
                  cGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGaa                                                                             <  2:3040580/100‑1 (MQ=255)
                   gTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAAt                                                                            >  2:2233836/1‑100 (MQ=255)
                   gTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAAt                                                                            >  1:3217280/1‑100 (MQ=255)
                     cAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATgg                                                                          <  2:713983/100‑1 (MQ=255)
                      aGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATggg                                                                         <  2:3792592/100‑1 (MQ=255)
                      aGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATggg                                                                         >  1:403434/1‑100 (MQ=255)
                       gCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATgggg                                                                        <  2:3390234/100‑1 (MQ=255)
                       gCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATgggg                                                                        >  2:4760460/1‑100 (MQ=255)
                       gCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATgggg                                                                        <  2:2501735/100‑1 (MQ=255)
                       gCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATgggg                                                                        <  1:4277161/100‑1 (MQ=255)
                        cTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGgc                                                                       <  2:134411/100‑1 (MQ=255)
                            ggCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGgcgcgc                                                                   >  2:1537301/1‑100 (MQ=255)
                               tctGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCggg                                                                <  1:4340629/100‑1 (MQ=255)
                                 tGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCa                                                              >  2:2075584/1‑100 (MQ=255)
                                  ggTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAg                                                             >  1:3167827/1‑100 (MQ=255)
                                   gtgtAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGa                                                            <  2:3444438/100‑1 (MQ=255)
                                   gtgtAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGa                                                            <  1:2126455/100‑1 (MQ=255)
                                    tgtAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGAt                                                           <  1:964294/100‑1 (MQ=255)
                                    tgtAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGAt                                                           <  1:685759/100‑1 (MQ=255)
                                     gtAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGAtt                                                          >  1:3404458/1‑100 (MQ=255)
                                        aCGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTAc                                                       >  2:1389006/1‑100 (MQ=255)
                                         cGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTAcc                                                      <  2:1213513/100‑1 (MQ=255)
                                         cGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTAcc                                                      >  1:4774471/1‑100 (MQ=255)
                                         cGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTAcc                                                      >  1:2097520/1‑100 (MQ=255)
                                            cAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGt                                                   <  1:789130/100‑1 (MQ=255)
                                             aGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGtt                                                  <  2:41745/100‑1 (MQ=255)
                                              gCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTg                                                 >  1:4249249/1‑100 (MQ=255)
                                                cGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAg                                               <  2:4560074/100‑1 (MQ=255)
                                                 gTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGc                                              >  2:3256329/1‑100 (MQ=255)
                                                    gTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAg                                           >  2:30764/1‑100 (MQ=255)
                                                    gTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGGGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAg                                           >  1:1721418/1‑100 (MQ=255)
                                                     ttttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGt                                          >  2:3020964/1‑100 (MQ=255)
                                                      tttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTg                                         >  1:2530457/1‑100 (MQ=255)
                                                      tttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTg                                         <  1:31126/100‑1 (MQ=255)
                                                      tttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTg                                         <  2:1698484/100‑1 (MQ=255)
                                                       ttGTTAGCACTCGCGACGCTGGATGTGCCAACCGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGa                                        <  1:2059147/100‑1 (MQ=255)
                                                       ttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGa                                        >  1:4995841/1‑100 (MQ=255)
                                                        tGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGaa                                       >  1:3028214/1‑100 (MQ=255)
                                                         gTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGaaa                                      <  2:4194244/100‑1 (MQ=255)
                                                          ttAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAAt                                     <  2:1062312/100‑1 (MQ=255)
                                                           tAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATa                                    >  2:3960323/1‑100 (MQ=255)
                                                           tAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATa                                    <  1:4625833/100‑1 (MQ=255)
                                                            aGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGagatcg                                   >  1:3289038/1‑95 (MQ=255)
                                                            aGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATaa                                   >  2:5028167/1‑100 (MQ=255)
                                                             gCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAg                                  <  2:4367226/100‑1 (MQ=255)
                                                             gCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAg                                  <  2:3886814/100‑1 (MQ=255)
                                                             gCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAg                                  <  1:1415988/100‑1 (MQ=255)
                                                              cACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGt                                 >  1:3127533/1‑100 (MQ=255)
                                                              cACTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGt                                 <  2:1265233/100‑1 (MQ=255)
                                                                cTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTAt                               >  1:1967132/1‑100 (MQ=255)
                                                                cTCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTAt                               >  1:4542388/1‑100 (MQ=255)
                                                                 tCGCGACGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTAtt                              <  2:464395/100‑1 (MQ=255)
                                                                     gACGCTGGATGTGCCAAACGTGAAACTGCACGACGGGGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTAc                          <  2:358657/100‑1 (MQ=255)
                                                                      aCGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACa                         >  2:2690726/1‑100 (MQ=255)
                                                                       cGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAg                        >  2:1789374/1‑100 (MQ=255)
                                                                       cGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAg                        >  2:1169853/1‑100 (MQ=255)
                                                                       cGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAg                        <  1:1295546/100‑1 (MQ=255)
                                                                       cGCTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAg                        >  2:3781974/1‑100 (MQ=255)
                                                                         cTGGATGTGCCAAACGTGAAACTGCACGACGTCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGc                      >  1:4138929/1‑100 (MQ=255)
                                                                         cTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGc                      <  2:784909/100‑1 (MQ=255)
                                                                         cTGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGc                      >  1:3457685/1‑100 (MQ=255)
                                                                          tGGATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCa                     <  1:2272906/100‑1 (MQ=255)
                                                                           ggATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCaa                    <  2:3419566/100‑1 (MQ=255)
                                                                           ggATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCaa                    >  1:1144646/1‑100 (MQ=255)
                                                                           ggATGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCaa                    >  2:2122083/1‑100 (MQ=255)
                                                                             aTGTGCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATa                  <  2:1991717/100‑1 (MQ=255)
                                                                               gtgCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATaaa                <  1:2310857/100‑1 (MQ=255)
                                                                               gtgCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATaaa                >  1:3791199/1‑100 (MQ=255)
                                                                                tgCCAAACGTGATACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCTGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATaaaa               >  2:4897057/1‑100 (MQ=255)
                                                                                tgCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATaaaa               >  1:4901348/1‑100 (MQ=255)
                                                                                 gCCAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATaaaaa              <  2:1942417/100‑1 (MQ=255)
                                                                                   cAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAAc            >  2:4477224/1‑100 (MQ=255)
                                                                                   cAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAAc            <  1:1382664/100‑1 (MQ=255)
                                                                                   cAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAAc            <  1:1863141/100‑1 (MQ=255)
                                                                                   cAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAAc            <  1:952686/100‑1 (MQ=255)
                                                                                   cAAACGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAAc            <  1:4713454/100‑1 (MQ=255)
                                                                                   cAAACGTGAAACTGCACGACGGCGCATGGAGGGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGCATTTTACAGGCAATAaacaac            >  1:2365597/1‑100 (MQ=255)
                                                                                    tatcGTGAAACGGCACGACGGTTCTGTGAGGGAAGGGGGCGCGCTGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAAcc           <  1:5165310/97‑1 (MQ=255)
                                                                                       cGTGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAAccgcc        <  1:539327/100‑1 (MQ=255)
                                                                                         tGAAACTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGa      >  1:922237/1‑100 (MQ=255)
                                                                                             aCTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt  <  2:4091084/100‑1 (MQ=255)
                                                                                             aCTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt  >  2:683326/1‑100 (MQ=255)
                                                                                             aCTGCACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTCGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt  >  1:5113827/1‑100 (MQ=255)
                                                                                                 |                                                                                               
CTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTT  >  NC_000913/2653180‑2653372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: