Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,779,038 G→A A1230V (GCC→GTC)  ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,779,0380GA99.4% 585.1 / NA 167A1230V (GCC→GTC) ypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (90/76);  minor base C (1/0);  total (91/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

GTTGTGTGGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGGCTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCGG  >  NC_000913/2778941‑2779134
                                                                                                 |                                                                                                
gttgtGTGGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACt                                                                                                >  1:3282257/1‑100 (MQ=255)
gttgtGTGGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACt                                                                                                >  2:3285145/1‑100 (MQ=255)
 ttgtGTGGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTg                                                                                               <  1:2610623/100‑1 (MQ=255)
  tgtgtgGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTga                                                                                              >  2:2580661/1‑99 (MQ=255)
  tgtgtgGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGc                                                                                              >  2:3781265/1‑100 (MQ=255)
  tgtgtgGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGc                                                                                              >  2:2120487/1‑100 (MQ=255)
  tgtgtgGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACCGTAATGTTGCTGCCATATTGAGTACGACTGc                                                                                              <  1:4141162/100‑1 (MQ=255)
    tgtgGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCg                                                                                            <  1:4809890/100‑1 (MQ=255)
      tgGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTa                                                                                          >  1:5087897/1‑100 (MQ=255)
      tgGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTa                                                                                          <  1:5128927/100‑1 (MQ=255)
       ggACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAg                                                                                         <  2:4757172/100‑1 (MQ=255)
          cTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGTGTACGACTGCCGTAGAAg                                                                                      >  2:2193234/1‑100 (MQ=255)
          cTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAg                                                                                      <  1:4667184/100‑1 (MQ=255)
           tCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAgg                                                                                     <  1:4712702/100‑1 (MQ=255)
            cGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGc                                                                                    >  1:541358/1‑100 (MQ=255)
            cGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGc                                                                                    >  1:541081/1‑100 (MQ=255)
            cGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGc                                                                                    >  1:2903731/1‑100 (MQ=255)
              cTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGt                                                                                  <  2:2350285/100‑1 (MQ=255)
              cTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGt                                                                                  <  2:455462/100‑1 (MQ=255)
              cTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGt                                                                                  <  2:4722703/100‑1 (MQ=255)
               tttCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGAGTGCCGTAGAAGGCGTa                                                                                 >  2:4258537/1‑100 (MQ=255)
               tttCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTa                                                                                 <  2:2880834/100‑1 (MQ=255)
                  cATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATg                                                                              >  2:2456606/1‑100 (MQ=255)
                  cATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATg                                                                              >  2:3203191/1‑100 (MQ=255)
                      aTGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGtt                                                                          <  2:533659/100‑1 (MQ=255)
                      aTGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGtt                                                                          <  1:2466567/100‑1 (MQ=255)
                      aTGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGtn                                                                          <  1:427588/100‑2 (MQ=255)
                      aTGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTCAGTACGACTGCCGTAGAAGGCGTAATGCGtt                                                                          <  2:4832878/100‑1 (MQ=255)
                       tGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGttt                                                                         >  2:4391825/1‑100 (MQ=255)
                       tGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGttt                                                                         >  2:837935/1‑100 (MQ=255)
                           cAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTcc                                                                     <  1:176205/100‑1 (MQ=255)
                           cAACCGCTCGCAAATACTGTTTAGCTCAGCATCAAATACCAGCCGTAATGTTGCTGCCATATGCAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTcc                                                                     <  1:112306/100‑1 (MQ=255)
                            aaCCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCg                                                                    >  1:675940/1‑100 (MQ=255)
                             aCCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCgg                                                                   <  1:2304411/100‑1 (MQ=255)
                                gCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAAc                                                                <  1:1835602/100‑1 (MQ=255)
                                  tCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACgn                                                              <  1:1895035/100‑2 (MQ=255)
                                   cgcgAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACggg                                                             >  1:244588/1‑100 (MQ=255)
                                   cgcgAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACggg                                                             >  1:4362945/1‑100 (MQ=255)
                                    gcgAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGgt                                                            <  1:4319928/100‑1 (MQ=255)
                                     cgAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGgtg                                                           <  1:2146616/100‑1 (MQ=255)
                                     cgAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGgtg                                                           <  2:1974563/100‑1 (MQ=255)
                                     cgAATACTGTTTAGCTCAGCAAAAAATACCAAGGGTAATGTTGAGGCCATATGTAGTATGCGTGCCGTAGATGGCGTAATGAGTTTTTCCGGAACGGgtg                                                           >  2:180714/1‑100 (MQ=255)
                                         tACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCgg                                                       <  1:205294/100‑1 (MQ=255)
                                          aCTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGc                                                      >  2:3365468/1‑100 (MQ=255)
                                           cTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCg                                                     <  2:306686/100‑1 (MQ=255)
                                            tGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGt                                                    >  1:928417/1‑100 (MQ=255)
                                            tGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGt                                                    <  1:5000441/100‑1 (MQ=255)
                                              tttAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTg                                                  <  1:399288/100‑1 (MQ=255)
                                               ttAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTgg                                                 <  1:4986216/100‑1 (MQ=255)
                                                  gCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAATGGGTGTCGGCGTTGGATc                                              >  2:1093184/1‑100 (MQ=255)
                                                   cTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGCGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCg                                             >  2:3761027/1‑100 (MQ=255)
                                                    tCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCgg                                            >  2:1918814/1‑100 (MQ=255)
                                                     cAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCggg                                           <  1:1309333/100‑1 (MQ=255)
                                                     cAGCATCAAAGACCAACGGTAATGTTGCGGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCTGAACGGGTGTCGGCGTTGGATCggg                                           <  2:4043857/100‑1 (MQ=255)
                                                       gCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAg                                         >  2:2691249/1‑100 (MQ=255)
                                                       gCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAg                                         >  1:4454391/1‑100 (MQ=255)
                                                       gCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAg                                         >  1:1391169/1‑100 (MQ=255)
                                                       gCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGGACGACTGCCGGAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGGTGGATCGGGAg                                         >  2:231962/1‑100 (MQ=255)
                                                         aTCAAATACCAGCGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTa                                       <  1:1446048/100‑1 (MQ=255)
                                                          tCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAg                                      >  1:2316014/1‑100 (MQ=255)
                                                           cAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGCTGGATCGGGAGTAgg                                     >  1:4004220/1‑100 (MQ=255)
                                                           aaaaCACCAACGGTAATGTTGCTGCCATATTAAGTACGAAAAACGTAGAAGGCGTAAAGCGTTTCTACGGAAAGGGAGTCGTAGTAAAATCGGGAGTAgg                                     >  2:4305130/2‑100 (MQ=255)
                                                            aaaTACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAggg                                    <  1:2433667/100‑1 (MQ=255)
                                                              atctcAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTc                                  <  1:1513894/96‑1 (MQ=255)
                                                              aTACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTc                                  <  1:1393470/100‑1 (MQ=255)
                                                              aTACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTc                                  <  1:785047/100‑1 (MQ=255)
                                                              aTACCAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTc                                  >  1:3009077/1‑100 (MQ=255)
                                                                 ccAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTgg                               >  2:1437485/1‑100 (MQ=255)
                                                                 ccAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTgg                               >  2:4911907/1‑100 (MQ=255)
                                                                 ccAACGGTAATGTTGCTGCCAAATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTgg                               >  1:2726388/1‑100 (MQ=255)
                                                                  cAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTcagat                              >  2:1492267/1‑96 (MQ=255)
                                                                  cAACGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTgga                              >  1:319552/1‑99 (MQ=255)
                                                                   aaCGGTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGtt                             <  2:1906631/100‑1 (MQ=255)
                                                                     cGGTAATGTTGGTGCCATATTGAGCACGAGTGCCGTAGAAGGCGTAATGCGTTTCTCCGGGACGGGTGTCGGCATTGGAGCGGGAGTAGGGTCTGGtttt                           >  1:4819149/1‑100 (MQ=255)
                                                                      ggTAAGGATGCTGCCAGATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGGGTCAACGTTGGATCGGGAGTAGGGTCgggaattg                          >  2:513324/1‑95 (MQ=255)
                                                                       gTAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTgg                         >  2:2081611/1‑100 (MQ=255)
                                                                        tAATGTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTggn                        <  1:4094548/100‑2 (MQ=255)
                                                                            gTTGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTg                    <  1:4573632/100‑1 (MQ=255)
                                                                             ttGCTGCCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTgg                   >  1:1105135/1‑100 (MQ=255)
                                                                              tgctgcCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTggg                  <  2:599341/100‑1 (MQ=255)
                                                                              tgctgcCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTggg                  >  1:4509741/1‑100 (MQ=255)
                                                                               gctacCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGt                 >  1:3969378/1‑100 (MQ=255)
                                                                                ctgcCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTc                <  1:1943350/100‑1 (MQ=255)
                                                                                  gcCATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTg              >  1:803852/1‑100 (MQ=255)
                                                                                    cATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGc            >  2:2508620/1‑100 (MQ=255)
                                                                                    cATATTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGc            >  2:4877612/1‑100 (MQ=255)
                                                                                     atatTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCt           <  1:870769/100‑1 (MQ=255)
                                                                                     atatTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCt           <  1:3048746/100‑1 (MQ=255)
                                                                                     atatTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCt           <  1:4760163/100‑1 (MQ=255)
                                                                                      tatTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCtt          >  1:4204898/1‑100 (MQ=255)
                                                                                      tatTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCtt          >  1:2056439/1‑100 (MQ=255)
                                                                                      tatTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGGCTGGTTTTGGATTTGGGTCGGGCtt          >  2:793544/1‑100 (MQ=255)
                                                                                       atTGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCttt         <  1:3174579/100‑1 (MQ=255)
                                                                                        ttGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTg        <  2:1145981/100‑1 (MQ=255)
                                                                                        ttGAGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTg        <  1:1883902/100‑1 (MQ=255)
                                                                                          gAGTAAGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGa      >  2:4466404/1‑100 (MQ=255)
                                                                                           aGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGCGCAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGAt     >  2:4589173/1‑100 (MQ=255)
                                                                                           aGTACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGAt     >  1:3714189/1‑100 (MQ=255)
                                                                                             tACGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCg   <  2:2867975/100‑1 (MQ=255)
                                                                                              aCGACTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCgg  >  1:2861051/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTTGTGTGGACTCGCTTTCATTATGTTCAACCGCTCGCGAATACTGTTTAGCTCAGCATCAAATACCAACGGTAATGTTGCTGCCATATTGAGTACGGCTGCCGTAGAAGGCGTAATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCGG  >  NC_000913/2778941‑2779134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: