BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,024,934	T→C	L195S (TTA→TCA)	xanQ →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,024,934	0	T	C	99.0%	686.1 / ‑5.1	197	L195S (TTA→TCA)	xanQ	xanthine permease
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (107/88); total (108/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

TGATTAAAGTCGGCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTTAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATG  >  NC_000913/3024835‑3025030

    tAAAGTCGGCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAAtt                                                                                              <  1:972964/100‑1 (MQ=255)

     aaaGTCGGCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATtg                                                                                             >  1:2855158/1‑100 (MQ=255)

        gTCGGCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTgtgg                                                                                          <  2:1533708/100‑1 (MQ=255)

        gTCGGCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTgtgg                                                                                          <  1:274806/100‑1 (MQ=255)

        gTCGGCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGCTGGTTTATTGGTTTCAATTgtgg                                                                                          >  2:3040631/1‑100 (MQ=255)

          cGGCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTgtggtg                                                                                        >  2:17116/1‑100 (MQ=255)

           ggCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGa                                                                                       >  2:543004/1‑100 (MQ=255)

              aTTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCg                                                                                    <  2:2809725/100‑1 (MQ=255)

                tATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGc                                                                                  >  2:1947312/1‑100 (MQ=255)

                 aTCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCt                                                                                 <  2:1993373/100‑1 (MQ=255)

                  tCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCtt                                                                                <  2:646381/100‑1 (MQ=255)

                   cGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCttt                                                                               <  2:1052510/100‑1 (MQ=255)

                   cGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCttt                                                                               >  2:3376290/1‑100 (MQ=255)

                    gATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTa                                                                              >  2:3746321/1‑100 (MQ=255)

                    gATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTa                                                                              <  1:4580432/100‑1 (MQ=255)

                     aTTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTaa                                                                             >  2:316847/1‑100 (MQ=255)

                      ttttGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAAc                                                                            <  2:1353158/100‑1 (MQ=255)

                       tttGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAAct                                                                           <  2:214157/100‑1 (MQ=255)

                         tggtggAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAActgc                                                                         <  1:869140/100‑1 (MQ=255)

                         tggtggAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAActgc                                                                         <  1:3206835/100‑1 (MQ=255)

                          ggtggAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAActgct                                                                        >  1:3654620/1‑100 (MQ=255)

                           gtggAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAActgctg                                                                       <  1:1301407/100‑1 (MQ=255)

                           gtggAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAActgctg                                                                       >  2:3809096/1‑100 (MQ=255)

                            tggAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGt                                                                      >  1:586406/1‑100 (MQ=255)

                            tggAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGt                                                                      >  1:2234631/1‑100 (MQ=255)

                             ggaggaTTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTc                                                                     >  2:838701/1‑100 (MQ=255)

                             ggaggaTTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTc                                                                     <  1:635017/100‑1 (MQ=255)

                              gaggaTTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTcn                                                                    <  1:4120262/100‑2 (MQ=255)

                              gaggaTTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCg                                                                    <  1:2886869/100‑1 (MQ=255)

                              gaggaTTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCg                                                                    <  1:2771562/100‑1 (MQ=255)

                                ggaTTTGCAGCCAAAAGCAGCGGTTCGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCa                                                                  <  2:2496431/100‑1 (MQ=255)

                                ggaTTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCa                                                                  >  2:429024/1‑100 (MQ=255)

                                   tttGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTc                                                               <  2:2823205/100‑1 (MQ=255)

                                       cAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGtt                                                           >  2:1782615/1‑100 (MQ=255)

                                       cAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGtt                                                           >  1:3310178/1‑100 (MQ=255)

                                        aGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCTTTg                                                          <  1:3972519/100‑1 (MQ=255)

                                         gCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGc                                                         <  1:4762299/100‑1 (MQ=255)

                                         gCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGc                                                         >  1:1131605/1‑100 (MQ=255)

                                         gCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGc                                                         >  1:1879248/1‑100 (MQ=255)

                                           cAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTa                                                       >  1:5063240/1‑100 (MQ=255)

                                           cAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTa                                                       >  2:4226000/1‑100 (MQ=255)

                                             aaaGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACg                                                     >  1:767620/1‑100 (MQ=255)

                                              aaGCAGCGGTACGTTCGGCACTTACGAACATCTCGGAGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGGTACGc                                                    >  2:618929/1‑100 (MQ=255)

                                               agcagcGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCa                                                   >  1:424921/1‑100 (MQ=255)

                                                 cagcGGTACGTTCAGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATg                                                 >  1:3065996/1‑100 (MQ=255)

                                                   gcGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATggg                                               <  2:4660277/100‑1 (MQ=255)

                                                    cGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATggga                                              <  1:3850606/100‑1 (MQ=255)

                                                     ggTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATgggag                                             >  1:3589140/1‑100 (MQ=255)

                                                      gTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATgggagg                                            <  2:3333588/100‑1 (MQ=255)

                                                      gTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATgggagg                                            <  1:3787295/100‑1 (MQ=255)

                                                        aCGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAATTGGTGTCGCAGTCCGTTGCTACTGATgggagggg                                          >  1:4351271/1‑99 (MQ=255)

                                                          gTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATc                                        <  2:355881/100‑1 (MQ=255)

                                                          gTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATc                                        <  2:1588756/100‑1 (MQ=255)

                                                          gTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATc                                        <  1:3512927/100‑1 (MQ=255)

                                                             cGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGcc                                     <  2:2942396/100‑1 (MQ=255)

                                                              ggCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCa                                    >  2:1672307/1‑100 (MQ=255)

                                                                cAATTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCAtt                                  >  2:1385797/1‑100 (MQ=255)

                                                                 aaTTACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTg                                 <  1:1949796/100‑1 (MQ=255)

                                                                   ttACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGCTCGCCATTggg                               >  2:3898947/1‑100 (MQ=255)

                                                                    tACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGc                              >  2:3370589/1‑100 (MQ=255)

                                                                    tACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGc                              <  1:4430806/100‑1 (MQ=255)

                                                                    tACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGc                              >  1:1322621/1‑100 (MQ=255)

                                                                    tACGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGGCGGTTGCTACGCATGGGAGGGATCGCCATTGGGc                              <  2:1482785/100‑1 (MQ=255)

                                                                    ctcGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGc                              <  1:4136826/98‑1 (MQ=255)

                                                                     aCGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCt                             >  1:3796450/1‑100 (MQ=255)

                                                                     aCGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCt                             >  1:3820708/1‑100 (MQ=255)

                                                                      cGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGcag                            >  2:4089076/1‑98 (MQ=255)

                                                                      cGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTa                            >  1:1645809/1‑100 (MQ=255)

                                                                      cGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTa                            >  2:4601348/1‑100 (MQ=255)

                                                                      cGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTa                            >  2:4915663/1‑100 (MQ=255)

                                                                      cGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTa                            <  1:2559942/100‑1 (MQ=255)

                                                                      cGAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTa                            >  2:2317634/1‑100 (MQ=255)

                                                                       gAACATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTAt                           >  1:840096/1‑100 (MQ=255)

                                                                        aaCATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGAGCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTAtg                          <  1:1806163/100‑1 (MQ=255)

                                                                         aCATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTAAGCATGGGAGGGATCGCCATTGGGCTAtgt                         >  1:4855501/1‑100 (MQ=255)

                                                                          cATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTAtgtg                        <  1:1178977/100‑1 (MQ=255)

                                                                          cATCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTAtgtg                        >  2:2037402/1‑100 (MQ=255)

                                                                           aTCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATgtgt                       <  1:1010025/100‑1 (MQ=255)

                                                                           aTCTCGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTACGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATgtgt                       >  2:167987/1‑100 (MQ=255)

                                                                            tctcGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTc                      >  1:2041328/1‑100 (MQ=255)

                                                                            tctcGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTc                      >  2:2681216/1‑100 (MQ=255)

                                                                             ctcGGCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGCCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCg                     >  2:4992173/1‑100 (MQ=255)

                                                                                 gCGTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGCGGGTTCGCCGTTGGGCGACGTGTCGGCta                 >  2:4534650/1‑100 (MQ=255)

                                                                                   gTTGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCtata               >  2:4834927/1‑100 (MQ=255)

                                                                                    ttGGTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTatat              <  1:3030048/100‑1 (MQ=255)

                                                                                    ttGGTATATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTatat              >  2:4463574/1‑100 (MQ=255)

                                                                                      ggTTTATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCg            <  1:4496527/100‑1 (MQ=255)

                                                                                        tttATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCa          >  1:3360497/1‑100 (MQ=255)

                                                                                         ttATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCAt         <  2:4020912/100‑1 (MQ=255)

                                                                                          tATTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATc        >  1:1424513/1‑100 (MQ=255)

                                                                                           aTTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCg       >  2:2957517/1‑100 (MQ=255)

                                                                                           aTTGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCg       >  1:705241/1‑100 (MQ=255)

                                                                                             tGGTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGtt     >  2:3410045/1‑100 (MQ=255)

                                                                                              ggTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTa    >  2:851032/1‑100 (MQ=255)

                                                                                              ggTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTa    >  2:2586365/1‑100 (MQ=255)

                                                                                              ggTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTa    >  2:1915311/1‑100 (MQ=255)

                                                                                              ggTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTa    >  1:4675265/1‑100 (MQ=255)

                                                                                               gTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTAt   >  2:4746732/1‑100 (MQ=255)

                                                                                               gTTTCAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTAt   >  1:365212/1‑100 (MQ=255)

                                                                                                tttcAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATg  >  2:1141243/1‑100 (MQ=255)

TGATTAAAGTCGGCATTATCGATTTTGGTGGAGGATTTGCAGCCAAAAGCAGCGGTACGTTCGGCAATTACGAACATCTCGGCGTTGGTTTATTGGTTTTAATTGTGGTGATCGGCTTTAACTGCTGTCGCAGTCCGTTGCTACGCATGGGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATG  >  NC_000913/3024835‑3025030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`