Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,136,947 C→T V526M (GTG→ATG)  gss ← glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,136,9470CT99.0% 701.6 / ‑2.9 206V526M (GTG→ATG) gssglutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (87/115);  total (89/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

Only 100 of 209 total aligned reads displayed.

CCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCTTTGTTGCCGGGGA  >  NC_000913/3136850‑3137042
                                                                                                 |                                                                                               
ccGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTtcatca                                                                                               <  2:1958083/100‑1 (MQ=255)
 cGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAg                                                                                              >  1:457173/1‑100 (MQ=255)
 cGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAg                                                                                              <  1:3621144/100‑1 (MQ=255)
 cGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAg                                                                                              <  1:1394471/100‑1 (MQ=255)
 cGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAg                                                                                              <  1:1975045/100‑1 (MQ=255)
    tGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTtc                                                                                           <  2:3720933/100‑1 (MQ=255)
     ggTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTtca                                                                                          <  1:2701329/100‑1 (MQ=255)
     ggTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTtca                                                                                          <  2:5032809/100‑1 (MQ=255)
      gTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTtcat                                                                                         <  1:3140072/100‑1 (MQ=255)
       ttttGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTtcatc                                                                                        >  1:3423040/1‑100 (MQ=255)
       ttttGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTtcatc                                                                                        >  1:3357522/1‑100 (MQ=255)
       ttttGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTtcatc                                                                                        <  2:1935084/100‑1 (MQ=255)
        tttGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTtcatca                                                                                       <  2:3289874/100‑1 (MQ=255)
        tttGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTtcatca                                                                                       <  1:2377666/100‑1 (MQ=255)
         ttGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTcatcat                                                                                      <  2:160097/100‑1 (MQ=255)
         ttGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTcatcat                                                                                      <  1:3267733/100‑1 (MQ=255)
          tGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCAtt                                                                                     >  2:592408/1‑100 (MQ=255)
           gTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTa                                                                                    <  1:2054268/100‑1 (MQ=255)
           gTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTa                                                                                    >  1:2918232/1‑100 (MQ=255)
           gTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTa                                                                                    <  1:2761940/100‑1 (MQ=255)
           gTCCAGCACCTCTGCATGACGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATAGGTCTCACTGCGTAACCTGTTTTCATCAGTTCATCATTa                                                                                    <  2:886473/100‑1 (MQ=255)
            tCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTaa                                                                                   >  2:2897862/1‑100 (MQ=255)
               aGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAg                                                                                <  2:3738403/100‑1 (MQ=255)
                gCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACaag                                                                               >  2:628477/1‑98 (MQ=255)
                gCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGt                                                                               <  1:4462116/100‑1 (MQ=255)
                  aCCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGa                                                                             <  1:15882/100‑1 (MQ=255)
                    ctctTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGaaa                                                                           <  2:3463621/100‑1 (MQ=255)
                      ctTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATc                                                                         >  2:1747316/1‑100 (MQ=255)
                      ctTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATc                                                                         <  1:1893176/100‑1 (MQ=255)
                      ctTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATc                                                                         >  2:4490454/1‑100 (MQ=255)
                      ctTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATc                                                                         >  1:5119105/1‑100 (MQ=255)
                        tCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCgg                                                                       <  1:5021763/100‑1 (MQ=255)
                        tCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCgg                                                                       <  1:422886/100‑1 (MQ=255)
                          atgatgGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGAGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTa                                                                     <  2:1181707/100‑1 (MQ=255)
                           tgatgGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTAt                                                                    <  2:3698882/100‑1 (MQ=255)
                           tgatgGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTAt                                                                    <  1:3509394/100‑1 (MQ=255)
                            gatgGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATc                                                                   >  1:1246528/1‑100 (MQ=255)
                              tgGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCca                                                                 <  2:1150101/100‑1 (MQ=255)
                                gatctcGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCcagc                                                               <  1:4534017/95‑1 (MQ=255)
                                 gtGACGAGGTCGATCTTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACCGTGAAATCGGTATCcagca                                                              <  2:4650345/99‑1 (MQ=255)
                                  tGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCcagcag                                                             >  2:694112/1‑100 (MQ=255)
                                  tGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCcagcag                                                             <  1:609154/100‑1 (MQ=255)
                                   tactaGGTCGATATTGCTGCCACAGCGACCGGCGCTCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAgg                                                            <  2:4173342/96‑1 (MQ=255)
                                   gACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAgg                                                            <  1:1310822/100‑1 (MQ=255)
                                     cGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCcagcagatc                                                          >  2:4460035/1‑97 (MQ=255)
                                       aGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATTGGTATCCAGCAGGTAAc                                                        <  1:1407072/100‑1 (MQ=255)
                                       aGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAAc                                                        <  2:3865923/100‑1 (MQ=255)
                                       aGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAAc                                                        <  1:59913/100‑1 (MQ=255)
                                        ggTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAAcn                                                       <  1:796479/100‑2 (MQ=255)
                                        ggTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACg                                                       >  2:3030332/1‑100 (MQ=255)
                                           cGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATg                                                    >  1:4480067/1‑100 (MQ=255)
                                            gATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATgg                                                   <  2:2720085/100‑1 (MQ=255)
                                             atatTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGt                                                  <  2:1348827/100‑1 (MQ=255)
                                             atatTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGt                                                  <  2:2514658/100‑1 (MQ=255)
                                               atTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGc                                                >  1:2881362/1‑100 (MQ=255)
                                                 tgctgcCACAGTGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCgg                                              >  2:4927043/1‑100 (MQ=255)
                                                 tgctgcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCgg                                              <  1:2450364/100‑1 (MQ=255)
                                                 tgccgcCACTGCCACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCgg                                              <  2:3179553/100‑1 (MQ=255)
                                                  gctgcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCggg                                             >  2:1110339/1‑100 (MQ=255)
                                                  gctgcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCggg                                             >  2:2473224/1‑100 (MQ=255)
                                                  gctgcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCggg                                             >  1:2598570/1‑100 (MQ=255)
                                                   ctgcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGa                                            <  1:3472240/100‑1 (MQ=255)
                                                   ctgcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATGAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGa                                            <  2:4427180/100‑1 (MQ=255)
                                                   ctgcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCAGCATTAACAGTGAAATCGGAATCCAGCTGGTAACGATGGTGCGGGa                                            >  1:4826684/1‑100 (MQ=255)
                                                    tgcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGaa                                           >  1:1548637/1‑100 (MQ=255)
                                                     gcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAAc                                          >  1:747450/1‑100 (MQ=255)
                                                     gcCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAAc                                          <  2:1012061/100‑1 (MQ=255)
                                                            ctaCCGGCGAGCTCGTTGACGGCGTAACCTGTGTTCAGCAGTGCATCATTAGCAAGGAAATCGCTATCCAGCAGGTAACGATGGTGCGCGAACAGCGAcc                                   <  2:4830798/98‑1 (MQ=255)
                                                            cGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGAcc                                   <  2:1224312/100‑1 (MQ=255)
                                                            cGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGAcc                                   <  1:2277428/100‑1 (MQ=255)
                                                            cGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGAcc                                   <  1:4654970/100‑1 (MQ=255)
                                                               ccGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCaga                                >  1:2119861/1‑100 (MQ=255)
                                                                cGGCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCagag                               <  1:3644558/100‑1 (MQ=255)
                                                                 ggCGATCGTTTTCACTGCGTGAGCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTCTCCAGCAGGTAACGATGGTGCGGGAACAGCGACCagatg                              >  2:2181888/1‑98 (MQ=255)
                                                                 ggCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAgg                              >  2:2047760/1‑100 (MQ=255)
                                                                 ggCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCCGTATCCAGCAGGTAACGATGCTCCGGGAACAGCGACCAGAgg                              <  1:4392272/100‑1 (MQ=255)
                                                                  gCGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGa                             <  1:935467/100‑1 (MQ=255)
                                                                   cGATCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAACCGGTAGCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGAt                            <  2:59903/100‑1 (MQ=255)
                                                                    gctCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATc                           <  2:227814/98‑1 (MQ=255)
                                                                     aTCGGTTCCACTGCGTAACCGGTTTTCAACAGTTCATCATTACCGGTGAAATCGGTATCCAGCAGGTAAGGATGGTGCGGGGACAGCGACCAGAGGATCg                          >  2:4081635/1‑100 (MQ=255)
                                                                      tCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGTGGGAACAGCGACCAGAGGATCgg                         <  2:450926/100‑1 (MQ=255)
                                                                      tCGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCgg                         <  2:2103956/100‑1 (MQ=255)
                                                                       cGGTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGc                        <  1:39059/100‑1 (MQ=255)
                                                                        ggTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCa                       >  1:1459706/1‑100 (MQ=255)
                                                                        ggTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCa                       >  1:4490366/1‑100 (MQ=255)
                                                                        ggTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCa                       >  2:3369679/1‑100 (MQ=255)
                                                                        ggTTTCACTGCGTAACCTGTTTCCATCACTTCATCATTAACAGTGAAATCGACATCCACCAGGTAACGATGGTGCGGGAACAGCGACCCGAGCATCGGCa                       >  2:4592636/1‑100 (MQ=255)
                                                                         gTTTCACTGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAg                      <  1:4936735/100‑1 (MQ=255)
                                                                                tGCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCt               <  2:2093927/100‑1 (MQ=255)
                                                                                 gCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCtt              <  2:1597547/100‑1 (MQ=255)
                                                                                 gCGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCtt              >  1:1159350/1‑100 (MQ=255)
                                                                                  cGTAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCttt             <  2:3326114/100‑1 (MQ=255)
                                                                                    tAACCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCTttgt           <  1:2502167/100‑1 (MQ=255)
                                                                                     aaCCTGGTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGTAGGTAACGATGGTGCGCGAACAGCGACGAGAAGATCGGCAGAATCGCTttgtt          >  2:4800094/1‑100 (MQ=255)
                                                                                      aCCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGCCCAGAGGATCGGCAGAATCGCTttgttg         >  1:2204805/1‑100 (MQ=255)
                                                                                      aCCTGTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCTttgttg         <  1:1469231/100‑1 (MQ=255)
                                                                                          gTTTTCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCTTTGTTGCCgg     >  2:3580785/1‑100 (MQ=255)
                                                                                           ttttCATCAGTTCATCATTAACAGTGAAATCGTTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCTTTGTTGCCggg    <  1:4216717/100‑1 (MQ=255)
                                                                                           ttttCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCTTTGTTGCCggg    <  1:1736142/100‑1 (MQ=255)
                                                                                           ttttCATCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCTTTGTTGCCggg    <  2:4873533/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CCGCTGGTTTTGTCCAGCACCTCTTCATGATGGCTGACGAGGTCGATATTGCTGCCACAGCGACCGGCGATCGGTTTCACTGCGTAACCTGTTTTCACCAGTTCATCATTAACAGTGAAATCGGTATCCAGCAGGTAACGATGGTGCGGGAACAGCGACCAGAGGATCGGCAGAATCGCTTTGTTGCCGGGGA  >  NC_000913/3136850‑3137042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑