Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,173,566 G→C R611G (CGC→GGC)  parE ← DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,173,5660GC97.8% 812.7 / 0.9 233R611G (CGC→GGC) parEDNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base C (124/103);  total (128/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

TTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAA  >  NC_000913/3173467‑3173661
                                                                                                   |                                                                                               
tttGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCg                                                                                                 <  1:649168/100‑1 (MQ=255)
    cccGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGCGCCTttc                                                                                             <  2:893572/100‑1 (MQ=255)
    cccGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTttc                                                                                             <  2:1111207/100‑1 (MQ=255)
        gCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCg                                                                                         <  1:4636778/100‑1 (MQ=255)
          cATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGcc                                                                                       <  1:2980252/100‑1 (MQ=255)
          cATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGcc                                                                                       <  2:1593820/100‑1 (MQ=255)
          cATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGcc                                                                                       >  2:4518795/1‑100 (MQ=255)
            tCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcag                                                                                     >  1:3096516/1‑100 (MQ=255)
              cTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagca                                                                                   >  1:4348105/1‑100 (MQ=255)
               tGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcag                                                                                  >  2:3707373/1‑100 (MQ=255)
               tGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcag                                                                                  <  2:2032506/100‑1 (MQ=255)
               tGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcag                                                                                  <  2:3744058/100‑1 (MQ=255)
               tGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcag                                                                                  <  1:4129435/100‑1 (MQ=255)
               tGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGGTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcag                                                                                  >  1:499972/1‑100 (MQ=255)
               tGACCGGGCAATGTTCTTTCCTTTAAACCCCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcag                                                                                  <  2:4917025/100‑1 (MQ=255)
                gACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCcagcaga                                                                                 >  1:2057909/1‑99 (MQ=255)
                    gggCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCatat                                                                             >  1:1374093/1‑100 (MQ=255)
                     ggCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCCTCCGAGCCTTTCTTCGCCAGCAGCATATc                                                                            <  1:1012075/100‑1 (MQ=255)
                      gCAATGTTGTTTCCTTTAAACCTCAATCGCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATcc                                                                           <  2:1395211/100‑1 (MQ=255)
                      gCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATcc                                                                           <  2:361400/100‑1 (MQ=255)
                       cAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCca                                                                          <  2:1450954/100‑1 (MQ=255)
                       cAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCca                                                                          <  1:2850377/100‑1 (MQ=255)
                         aTGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCcatc                                                                        <  2:5061611/100‑1 (MQ=255)
                         aTGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCcatc                                                                        >  1:1689056/1‑100 (MQ=255)
                          tGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCcgtca                                                                       <  2:1915481/100‑1 (MQ=255)
                            ttCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCatcatc                                                                     <  1:1298567/100‑1 (MQ=255)
                              cTTTCCTTTGAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATcgc                                                                   <  2:754239/100‑1 (MQ=255)
                              cTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATcgc                                                                   >  1:4776165/1‑100 (MQ=255)
                               tttCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATcgcg                                                                  >  2:3499730/1‑100 (MQ=255)
                                 tCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTc                                                                >  2:169427/1‑100 (MQ=255)
                                  ccTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCa                                                               <  1:529178/100‑1 (MQ=255)
                                   cTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAg                                                              <  2:2616986/100‑1 (MQ=255)
                                   cTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAg                                                              <  1:2309533/100‑1 (MQ=255)
                                    tttAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGt                                                             >  2:2673892/1‑100 (MQ=255)
                                      tAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTAc                                                           >  1:3808363/1‑100 (MQ=255)
                                        aaCCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGc                                                         <  2:351574/100‑1 (MQ=255)
                                          ccTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                                                       >  1:1922072/1‑100 (MQ=255)
                                          ccTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                                                       >  2:3283811/1‑100 (MQ=255)
                                          ccTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                                                       >  2:3966140/1‑100 (MQ=255)
                                          ccTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                                                       >  2:217439/1‑100 (MQ=255)
                                          ccTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                                                       >  2:218736/1‑100 (MQ=255)
                                          ccTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                                                       >  1:3725451/1‑100 (MQ=255)
                                            tCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGAt                                                     >  2:2841601/1‑100 (MQ=255)
                                              gaTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCg                                                   <  2:2412439/99‑1 (MQ=255)
                                              aaTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCg                                                   <  1:1336042/100‑1 (MQ=255)
                                              aaTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCg                                                   <  2:1099686/100‑1 (MQ=255)
                                              aaTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCg                                                   <  1:2581681/100‑1 (MQ=255)
                                              aaTCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCg                                                   >  1:4027608/1‑100 (MQ=255)
                                                  tcCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCtt                                               >  1:3139934/1‑100 (MQ=255)
                                                   tcGCCATGTCGCTTTTCTCTTGCACCCATTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtc                                              <  2:3457300/99‑1 (MQ=255)
                                                   ccGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGAACGCTGATCGTCTtc                                              >  1:2568492/1‑100 (MQ=255)
                                                    cGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtca                                             <  1:2731671/100‑1 (MQ=255)
                                                      ccATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtcatc                                           >  1:2781452/1‑100 (MQ=255)
                                                        aTGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTcatcat                                         >  2:3826087/1‑100 (MQ=255)
                                                         tGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCTAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCatcatc                                        <  2:3557906/100‑1 (MQ=255)
                                                         tGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCatcatc                                        <  1:1777436/100‑1 (MQ=255)
                                                         tGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCatcatc                                        <  1:4148507/100‑1 (MQ=255)
                                                          gTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCg                                       >  1:4763465/1‑100 (MQ=255)
                                                           tCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTCCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGa                                      <  1:3356013/100‑1 (MQ=255)
                                                            cGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGAt                                     >  2:1886435/1‑100 (MQ=255)
                                                            cGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGAt                                     <  2:4328760/100‑1 (MQ=255)
                                                              ccTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAg                                   <  1:1094803/100‑1 (MQ=255)
                                                                 ttCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCa                                <  1:3680507/100‑1 (MQ=255)
                                                                 ttCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCa                                <  1:512685/100‑1 (MQ=255)
                                                                   ctctTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTATGCTGATCGTCTTCATCATCGATAGTCAAc                              >  1:3494494/1‑100 (MQ=255)
                                                                   ctctTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAAc                              >  1:2511724/1‑100 (MQ=255)
                                                                   ctctTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAAc                              <  2:2268356/100‑1 (MQ=255)
                                                                    tctTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACt                             >  1:4389011/1‑100 (MQ=255)
                                                                    tctTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACt                             >  1:910906/1‑100 (MQ=255)
                                                                    tctTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACt                             >  2:4119637/1‑100 (MQ=255)
                                                                     ctTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTg                            <  2:2461127/100‑1 (MQ=255)
                                                                     ctTGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTg                            <  2:3159478/100‑1 (MQ=255)
                                                                      ttGCAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCTTATCCATCATCGCGTCAGGACGCTGATCGTCTTCATCATCGATAGTCAACTGc                           <  2:4687419/100‑1 (MQ=255)
                                                                         cAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCAcc                        <  1:2409880/100‑1 (MQ=255)
                                                                         cAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCAcc                        >  2:3339989/1‑100 (MQ=255)
                                                                         cAACCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCAcc                        <  2:2116561/100‑1 (MQ=255)
                                                                           aCCAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGAATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCCTCTGCACCAg                      >  2:4029143/1‑100 (MQ=255)
                                                                            ccAGTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGa                     >  1:2201783/1‑100 (MQ=255)
                                                                               gTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACgg                  >  2:45661/1‑100 (MQ=255)
                                                                               gTTGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACgg                  >  1:3275654/1‑100 (MQ=255)
                                                                                 tGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCg                >  2:3920137/1‑100 (MQ=255)
                                                                                 tGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCg                >  2:576804/1‑100 (MQ=255)
                                                                                 tGCGGCGATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCg                <  2:4969262/100‑1 (MQ=255)
                                                                                  gcggcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGa               >  1:5047873/1‑100 (MQ=255)
                                                                                  gcggcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGa               >  2:4778833/1‑100 (MQ=255)
                                                                                  gcggcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGCCAGCACGCTGATCGCCTCCATCATCCATAGTCCACTGCACCAGACGGCGa               >  2:4413629/1‑100 (MQ=255)
                                                                                  gcggcgATCTTCCGAGCCTTGCTTCGCCATCAGCATATCCATCATCGCGTCAGTACGCTGAGCTTCTGCATCATCTATAGTCAACTGCACCAGACGGCGa               >  2:4077478/1‑100 (MQ=255)
                                                                                   cggcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAg              <  2:292331/100‑1 (MQ=255)
                                                                                   cggcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAg              >  2:866460/1‑100 (MQ=255)
                                                                                     gcgATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAgtg            >  1:1243754/1‑100 (MQ=255)
                                                                                       ggtCTTCCGAGGCTTTCTTCGCCAGCAGCATATCAATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGtt          >  2:4702272/3‑100 (MQ=255)
                                                                                       gATCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGtt          >  1:247700/1‑100 (MQ=255)
                                                                                         tCTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCACCTGCCCCAGACGGCGAGTGTTCg        <  2:3985816/100‑1 (MQ=255)
                                                                                          cTTCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCgg       >  2:2810120/1‑100 (MQ=255)
                                                                                           ttCCGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGa      >  1:217949/1‑100 (MQ=255)
                                                                                            tCCGAGCCTTTCTTCGCCAGCAGCATATCAATCATCGCGACAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGAt     <  2:2357036/100‑1 (MQ=255)
                                                                                             ccGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATc    >  2:1326893/1‑100 (MQ=255)
                                                                                              cGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCa   >  1:4565761/1‑100 (MQ=255)
                                                                                              cGAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCa   >  1:5062638/1‑100 (MQ=255)
                                                                                               gAGCCTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCaa  >  2:819338/1‑100 (MQ=255)
                                                                                                   |                                                                                               
TTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATCGATAGTCAACTGCACCAGACGGCGAGTGTTCGGATCAA  >  NC_000913/3173467‑3173661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑