Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,661,667 T→C R418R (CGT→CGC mdtF → anaerobic multidrug efflux transporter, ArcA‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,661,6670TC99.5% 630.9 / ‑4.8 183R418R (CGT→CGCmdtFanaerobic multidrug efflux transporter, ArcA‑regulated
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (97/85);  total (97/86)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

Only 100 of 185 total aligned reads displayed.

TCGGTTTCACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGTGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCGTTGTTCTTTCC  >  NC_000913/3661570‑3661763
                                                                                                 |                                                                                                
tcggtTTCACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCgcgt                                                                                                <  1:230550/100‑1 (MQ=255)
  ggtTTCACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCa                                                                                              <  1:4924616/100‑1 (MQ=255)
  ggtTTCACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCa                                                                                              >  1:3649168/1‑100 (MQ=255)
   gtTTCACCATCAACACGTTGAGTATGTTCGGGATGGTTCTGGCGATCGGGTTACTGGTGGATGACCCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCAt                                                                                             <  1:4796988/100‑1 (MQ=25)
   gtTTCACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCAt                                                                                             >  1:4821283/1‑100 (MQ=255)
     ttCACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTg                                                                                           >  1:1844620/1‑100 (MQ=255)
      tCACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGc                                                                                          <  1:1939880/100‑1 (MQ=255)
       cACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGCGACCGTCGAGCGCGTCATTGCg                                                                                         >  2:734839/1‑100 (MQ=255)
       cACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCg                                                                                         <  2:4070024/100‑1 (MQ=255)
           aTCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAg                                                                                     <  1:4275078/100‑1 (MQ=255)
            tCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGa                                                                                    <  1:833512/100‑1 (MQ=255)
              aaCACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATa                                                                                  <  2:1175334/100‑1 (MQ=255)
              aaCACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATa                                                                                  <  1:4238639/100‑1 (MQ=255)
               acacGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATaa                                                                                 >  1:1096273/1‑100 (MQ=255)
                     tGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTAcn                                                                           <  1:71/100‑2 (MQ=255)
                     tGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTAcc                                                                           >  1:285691/1‑100 (MQ=255)
                      gACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCCAccg                                                                          >  2:3531989/1‑100 (MQ=255)
                        cTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCTCGTCATTGCGGAAGATAAGCTAccgcc                                                                        <  2:4210248/100‑1 (MQ=255)
                        cTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTAccgcc                                                                        <  1:1058151/100‑1 (MQ=255)
                           tgtgCGGGATGGTGCTGACGATAGGGTTACTGGTGGATGACGCCATCATGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCgaa                                                                     >  2:1011343/1‑100 (MQ=37)
                           tGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCgaa                                                                     <  2:2469952/100‑1 (MQ=255)
                            gTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCgaag                                                                    <  2:1732721/100‑1 (MQ=255)
                            gTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCgaag                                                                    >  1:2083978/1‑100 (MQ=255)
                            gTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCgaag                                                                    <  1:1449587/100‑1 (MQ=255)
                            gTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATAACGCCATAGTGGTGGTGGAGAACGTCGCGCGCGTCATTGCGGAAGATAAGCTACCGCCcaag                                                                    <  2:29348/100‑1 (MQ=25)
                                gggATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCgaaggaag                                                                <  1:2845605/100‑1 (MQ=255)
                                gggATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCgaaggaag                                                                <  1:4453508/100‑1 (MQ=255)
                                 ggATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGc                                                               <  2:2080141/100‑1 (MQ=255)
                                 ggATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGc                                                               >  2:687638/1‑100 (MQ=255)
                                  gATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCg                                                              <  1:1703204/100‑1 (MQ=255)
                                     ggTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACg                                                           <  1:257058/100‑1 (MQ=255)
                                     ggTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACg                                                           <  1:3184966/100‑1 (MQ=255)
                                       tGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCa                                                         >  1:3714244/1‑100 (MQ=255)
                                       tGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCa                                                         >  1:3714655/1‑100 (MQ=255)
                                        gCTGTCGATAGGGGTACTGGTGGATGACGCCAGCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCTGAAGGGAGCGACGCAt                                                        >  2:958260/1‑100 (MQ=37)
                                         cTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATa                                                       <  1:3013479/100‑1 (MQ=255)
                                            gCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAAt                                                    <  1:1567419/100‑1 (MQ=255)
                                             cGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATc                                                   >  1:3856725/1‑100 (MQ=255)
                                             cGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATc                                                   >  2:1408000/1‑100 (MQ=255)
                                              gATAGGGTTACTGGTGGATGACGCCATCGTGGTGGGGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCg                                                  <  1:3314712/100‑1 (MQ=255)
                                                tcgggTCACTGCTGGATGCCCTCCTCGTGCTGGTGGAGACCGTCGAGCGCGTCATTGCGGAAGCTAAGCTACCGCCGAAGGAAGCGACGCATAAATCgct                                                <  2:2789186/98‑3 (MQ=255)
                                                 aGGGTTACTGGTGGATGACGTCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATg                                               <  1:4917835/100‑1 (MQ=255)
                                                  gggTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATgg                                              <  1:2717897/100‑1 (MQ=255)
                                                  gggTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATgg                                              >  1:2382866/1‑100 (MQ=255)
                                                   ggTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATggg                                             >  1:1238363/1‑100 (MQ=255)
                                                     ttACTGGTGGATGACGCCCTCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGc                                           <  1:4629097/100‑1 (MQ=255)
                                                     ttACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGc                                           <  2:2700327/100‑1 (MQ=255)
                                                     ttACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGc                                           <  2:1242455/100‑1 (MQ=255)
                                                       aCTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAg                                         >  2:1908941/1‑100 (MQ=255)
                                                        cTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTGCCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGa                                        >  2:4619419/1‑100 (MQ=255)
                                                        cTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGa                                        >  1:63642/1‑100 (MQ=255)
                                                          ggtggATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATc                                      >  2:395935/1‑100 (MQ=255)
                                                          ggtggATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATc                                      >  1:917546/1‑100 (MQ=255)
                                                          ggtggATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATc                                      >  1:2972074/1‑100 (MQ=255)
                                                          ggtggATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATc                                      >  2:3478053/1‑100 (MQ=255)
                                                          ggtggATGACGCCATCGTGGTGGTGAAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATc                                      >  2:1411872/1‑100 (MQ=255)
                                                           gtggATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATcc                                     <  2:2058312/100‑1 (MQ=255)
                                                              gATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAAc                                  >  1:3759667/1‑100 (MQ=255)
                                                               aTGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCGTTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACg                                 <  1:5109284/100‑1 (MQ=255)
                                                               aTGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACg                                 <  1:2749390/100‑1 (MQ=255)
                                                               aTGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACg                                 <  2:1536013/100‑1 (MQ=255)
                                                               aTGACGACATCGTGGTGGTGGAGAACGTCGGGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACg                                 <  2:4116023/100‑1 (MQ=255)
                                                                tGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGATGCGACGCATAAATCGATGGGGCAGATCCAACGt                                >  2:3664759/1‑100 (MQ=255)
                                                                tGACGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGt                                >  1:3979868/1‑100 (MQ=255)
                                                                 atctccATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTg                               <  2:3705695/96‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAGAACGTCGAGCGTGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTgc                              <  1:945181/100‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTgc                              >  2:2131613/1‑100 (MQ=255)
                                                                   cGCCATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTgcg                             >  1:498988/1‑100 (MQ=255)
                                                                     ccATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCt                           <  1:2121005/100‑1 (MQ=255)
                                                                     ccATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCt                           >  2:2146651/1‑100 (MQ=255)
                                                                     ccATCGTGGTGGTGGAGAAAGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGCTCCAACGTGCGCt                           >  2:1041643/1‑100 (MQ=255)
                                                                      cATCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTg                          <  1:3542000/100‑1 (MQ=255)
                                                                       aTCGTGGTGGTGGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTgg                         <  2:4819803/100‑1 (MQ=255)
                                                                       aTCGCGGTGGTGGCGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTgg                         >  2:4234084/1‑100 (MQ=255)
                                                                          gtggtggtgGAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCg                      >  1:3882867/1‑100 (MQ=255)
                                                                           tggtggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCgg                     <  1:2899656/100‑1 (MQ=255)
                                                                           tggtggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCgg                     <  2:4947812/100‑1 (MQ=255)
                                                                           tggtggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCgg                     <  2:3118944/100‑1 (MQ=255)
                                                                            ggtggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGt                    <  1:2490306/100‑1 (MQ=255)
                                                                             gtggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTa                   >  2:2234056/1‑100 (MQ=255)
                                                                              tggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTAt                  <  2:2358228/100‑1 (MQ=255)
                                                                              tggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTAt                  <  2:1524040/100‑1 (MQ=255)
                                                                              tggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTAt                  >  1:3389728/1‑100 (MQ=255)
                                                                              tggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTAt                  <  1:1144148/100‑1 (MQ=255)
                                                                               ggtggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTAtt                 >  2:2677747/1‑100 (MQ=255)
                                                                                 tggAGAACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGc               >  2:3888138/1‑100 (MQ=255)
                                                                                   gagaACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCg             <  2:3014147/100‑1 (MQ=255)
                                                                                    agaACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCgt            >  1:2278232/1‑100 (MQ=255)
                                                                                     gaACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCgtt           >  1:105680/1‑100 (MQ=255)
                                                                                     gaACGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCgtt           >  2:1052135/1‑100 (MQ=255)
                                                                                      aaCGTCGAGCGCGTCATTGCGGAAGAGAAGCTACCGCAGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCgttg          <  2:4611219/100‑1 (MQ=255)
                                                                                       aCGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGGGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCgttgt         <  2:299700/100‑1 (MQ=255)
                                                                                        cGTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCgttgtt        >  1:3282736/1‑100 (MQ=255)
                                                                                         gTCGAGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCGTTGTTc       <  1:473935/100‑1 (MQ=255)
                                                                                             aGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCGTTGTTCTTTc   >  2:244122/1‑100 (MQ=255)
                                                                                             aGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCGTTGTTCTTTc   <  2:1953301/100‑1 (MQ=255)
                                                                                             aGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCGTTGTTCTTTc   >  1:572362/1‑100 (MQ=255)
                                                                                             aGCGCGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCGTTGTTCTTTc   <  1:4698749/100‑1 (MQ=255)
                                                                                             aGCGCGTCAATGCGGAAAATAAGCTAACGCCGAAGGAAGCGACGCATAAATCGCTGGGGCAGATCCAACGTGCGCTGGTCGGTATTCGCGTTGTTCTTTc   <  2:193077/100‑1 (MQ=255)
                                                                                              gcgcGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCGTTGTTCTTTcc  >  1:1381165/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TCGGTTTCACCATCAACACGTTGACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCGTGGTGGTGGAGAACGTCGAGCGTGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCATAAATCGATGGGGCAGATCCAACGTGCGCTGGTCGGTATTGCCGTTGTTCTTTCC  >  NC_000913/3661570‑3661763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: