Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,135,739 T→C V635V (GTT→GTC)  katG → catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,135,7390TC98.7% 792.9 / ‑3.1 231V635V (GTT→GTC) katGcatalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (106/122);  total (106/125)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

CGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGAC  >  NC_000913/4135642‑4135835
                                                                                                 |                                                                                                
cGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGGGTGTACTGGGTGGCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCgg                                                                                                >  1:3626636/1‑100 (MQ=255)
  cTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCg                                                                                              <  2:812768/100‑1 (MQ=255)
  cTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCg                                                                                              <  1:4517321/100‑1 (MQ=255)
    gACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTa                                                                                            <  2:1800044/100‑1 (MQ=255)
     aCCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAt                                                                                           >  1:3247546/1‑100 (MQ=255)
      ccGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAtt                                                                                          >  1:403769/1‑100 (MQ=255)
      ccGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAtt                                                                                          <  1:1304103/100‑1 (MQ=255)
      ccGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAtt                                                                                          <  2:1649228/100‑1 (MQ=255)
      ccGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAtt                                                                                          <  2:3729540/100‑1 (MQ=255)
      ccGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTAtt                                                                                          <  1:2341932/100‑1 (MQ=255)
       cgcgCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTg                                                                                         <  1:361214/100‑1 (MQ=255)
       cgcgCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTg                                                                                         <  1:4598060/100‑1 (MQ=255)
       cgcgCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTg                                                                                         >  2:559021/1‑100 (MQ=255)
          gcCGGAAGTGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGTTGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGc                                                                                      <  2:4721335/100‑1 (MQ=255)
          gcCGGAAATGACTGCGCTGGTGTGCGGCATGCGTGTACTGGGTGCCAACTGCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTACTGAGc                                                                                      <  2:4882674/100‑1 (MQ=255)
          gcCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGc                                                                                      <  2:2443809/100‑1 (MQ=255)
          gcCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGc                                                                                      <  2:2209409/100‑1 (MQ=255)
          gcCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGc                                                                                      <  2:4016497/100‑1 (MQ=255)
           ccGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCa                                                                                     >  2:2997404/1‑100 (MQ=255)
           ccGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCa                                                                                     <  2:3211376/100‑1 (MQ=255)
           ccGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCa                                                                                     >  1:3790426/1‑100 (MQ=255)
              gAAATTACTTCGTTGGCCGGAAGCATGCTTGTACTGCTCTCCAACTTCTATGGCAGCAAAAACGTACTCTTCACTGACAACGTCCGCGTATTGAGCAATg                                                                                  <  2:1017801/100‑1 (MQ=255)
              gAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATg                                                                                  >  2:1651981/1‑100 (MQ=255)
               aaaTGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGa                                                                                 <  1:2143342/100‑1 (MQ=255)
                aaTGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGAc                                                                                <  2:426793/100‑1 (MQ=255)
                aaTGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGAc                                                                                <  2:4475614/100‑1 (MQ=255)
                 aTGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGAct                                                                               <  1:1674611/100‑1 (MQ=255)
                  tGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGTAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGActt                                                                              >  2:3041320/1‑100 (MQ=255)
                   gACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGActtc                                                                             <  1:3061308/100‑1 (MQ=255)
                    aCTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGActtct                                                                            <  1:5004789/100‑1 (MQ=255)
                     cTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGActtctt                                                                           >  2:832762/1‑100 (MQ=255)
                     cTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGActtctt                                                                           >  1:3034991/1‑100 (MQ=255)
                        cgcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGt                                                                        >  2:1290057/1‑100 (MQ=255)
                         gcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTg                                                                       <  1:3123837/100‑1 (MQ=255)
                         gcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTg                                                                       >  1:4255300/1‑100 (MQ=255)
                         gcTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTGCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTg                                                                       <  2:1521293/100‑1 (MQ=255)
                           tggtggGCGGCATGCGTGTACTTGTTGCCAATTTCGAGGGCAGCAAAAACGGCTTCTTCACTGACCGCGTCGGCGTATTGAGCAATGGCTTCTTCGTGaa                                                                     <  2:2271105/100‑1 (MQ=255)
                           tggtggGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGaa                                                                     <  1:1013505/100‑1 (MQ=255)
                             gtggGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACt                                                                   >  2:1080794/1‑100 (MQ=255)
                             gtggGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACt                                                                   >  1:5114715/1‑100 (MQ=255)
                                ggcggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGc                                                                >  2:1947800/1‑100 (MQ=255)
                                 gcggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCt                                                               >  1:840563/1‑100 (MQ=255)
                                  cggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTg                                                              <  1:4737371/100‑1 (MQ=255)
                                  cggcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTg                                                              <  1:1864716/100‑1 (MQ=255)
                                    gcATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGa                                                            >  1:1343970/1‑100 (MQ=255)
                                       tGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGatat                                                         >  1:1579324/1‑100 (MQ=255)
                                       tGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGatat                                                         <  2:5021407/100‑1 (MQ=255)
                                        gCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATg                                                        >  2:2118824/1‑100 (MQ=255)
                                        gCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATg                                                        <  1:2503825/100‑1 (MQ=255)
                                          gtgtACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCg                                                      <  1:3928399/100‑1 (MQ=255)
                                          gtgtACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCg                                                      <  1:2854345/100‑1 (MQ=255)
                                             tACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTa                                                   <  2:3796475/100‑1 (MQ=255)
                                              aCTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGACTTCACTGACCGAGTCGGCGTCTTAAGCAATGACTTCTTAGTGACCTTGCTGCATATGCgctcc                                                  >  2:2775522/1‑96 (MQ=255)
                                               cTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACg                                                 <  1:104352/100‑1 (MQ=255)
                                                 gggTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAg                                               <  2:2575583/100‑1 (MQ=255)
                                                 gggTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAg                                               >  1:1078850/1‑100 (MQ=255)
                                                     gCCAACTTCGATGGCAGCTACAACGGCGTCTTCACTGACTGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGa                                           <  1:4828248/100‑1 (MQ=255)
                                                     gCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGa                                           >  2:4571586/1‑100 (MQ=255)
                                                      ccAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGaa                                          >  2:775232/1‑100 (MQ=255)
                                                      ccAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGaa                                          <  2:1589546/100‑1 (MQ=255)
                                                       cAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGaaa                                         >  2:1313389/1‑100 (MQ=255)
                                                       cAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGaaa                                         >  1:201869/1‑100 (MQ=255)
                                                        aaCTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAg                                        <  1:3142580/100‑1 (MQ=255)
                                                         aCTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGc                                       <  1:2379207/100‑1 (MQ=255)
                                                         aCTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGc                                       <  1:1154691/100‑1 (MQ=255)
                                                          cTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcg                                      <  2:3892205/100‑1 (MQ=255)
                                                             cGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgacc                                   <  1:967299/100‑1 (MQ=255)
                                                              gATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgaccg                                  <  2:242045/100‑1 (MQ=255)
                                                              gATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgaccg                                  <  2:3325795/100‑1 (MQ=255)
                                                                tGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgaccgac                                <  1:3099362/100‑1 (MQ=255)
                                                                tGGCAGCAAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGcgaccgac                                <  1:1822420/100‑1 (MQ=255)
                                                                  gcagcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACcgacga                              >  1:2320211/1‑100 (MQ=255)
                                                                  gcagcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACcgacga                              >  1:733291/1‑100 (MQ=255)
                                                                   cagcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGaa                             >  2:2672453/1‑100 (MQ=255)
                                                                     gcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAGTGACTTCTTCGTGAACTTTCTGGATATGAGTTTCGAGTGGCAAGCGACGGATGAATc                           >  2:4787432/1‑100 (MQ=255)
                                                                     gcaAAAACGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGCCTTCTTCGTGAACTTGCTGGATATGCGTTAGGAGTGGAAAGGGACCGACGAATc                           >  1:4369868/1‑100 (MQ=255)
                                                                       aaaaaCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTCCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGa                         >  2:252755/1‑100 (MQ=255)
                                                                          aaCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAGCTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAag                      <  2:4204012/100‑1 (MQ=255)
                                                                          aaCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAag                      >  1:443335/1‑100 (MQ=255)
                                                                          aaCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAag                      <  1:2090945/100‑1 (MQ=255)
                                                                           aCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAaga                     >  1:192256/1‑100 (MQ=255)
                                                                           aCGGCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAaga                     >  2:2849109/1‑100 (MQ=255)
                                                                             ggCGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGc                   <  2:4342893/100‑1 (MQ=255)
                                                                             ggCGTCTTCACTGACCGCATCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAACGAGc                   <  2:4303993/100‑1 (MQ=255)
                                                                               cGTCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTg                 >  1:2947658/1‑100 (MQ=255)
                                                                                 tCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGtt               >  1:3403951/1‑100 (MQ=255)
                                                                                 tCTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGtt               >  2:640715/1‑100 (MQ=255)
                                                                                  cTTCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTc              >  1:1417283/1‑100 (MQ=255)
                                                                                   ttCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCg             >  1:547580/1‑100 (MQ=255)
                                                                                    tCACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGa            <  1:3070401/100‑1 (MQ=255)
                                                                                     cACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGaa           >  2:1198144/1‑100 (MQ=255)
                                                                                     cACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGaa           >  2:345554/1‑100 (MQ=255)
                                                                                     cACTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGaa           >  1:4311301/1‑100 (MQ=255)
                                                                                      aCTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAg          <  1:1499853/100‑1 (MQ=255)
                                                                                       cTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAgg         >  2:4172969/1‑100 (MQ=255)
                                                                                       cTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAgg         >  1:1185535/1‑100 (MQ=255)
                                                                                       cTGACCGCGTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAgg         <  2:30920/100‑1 (MQ=255)
                                                                                            cgcgTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtg    >  2:1888714/1‑100 (MQ=255)
                                                                                            cgcgTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtg    >  2:1865686/1‑100 (MQ=255)
                                                                                              cgTCGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGCGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGccgtgac  >  2:3645969/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGAGCAATGACTTCTTCGTGAACTTGCTGGATATGCGTTACGAGTGGAAAGCGACCGACGAATCGAAAGAGCTGTTCGAAGGCCGTGAC  >  NC_000913/4135642‑4135835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑