Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,438,767 C→T A166T (GCG→ACG)  ytfJ ← putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,438,7670CT97.8% 617.2 / ‑3.1 187A166T (GCG→ACG) ytfJputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (88/93);  total (90/97)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

AGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGCCATT  >  NC_000913/4438670‑4438863
                                                                                                 |                                                                                                
aGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGtcc                                                                                                <  1:5005104/100‑1 (MQ=255)
 ggggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGtccc                                                                                               >  2:2438384/1‑100 (MQ=255)
 ggggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGtccc                                                                                               >  2:3459699/1‑100 (MQ=255)
 ggggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGtccc                                                                                               >  1:510594/1‑100 (MQ=255)
  gggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCg                                                                                              >  1:2457785/1‑100 (MQ=255)
    gAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTc                                                                                            <  1:2498881/100‑1 (MQ=255)
      aaaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCtt                                                                                          <  1:3351840/100‑1 (MQ=255)
       aaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCttt                                                                                         <  1:2904684/100‑1 (MQ=255)
          tattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGc                                                                                      <  1:2611308/100‑1 (MQ=255)
           attTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGcc                                                                                     >  1:3808680/1‑100 (MQ=255)
           attTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGcc                                                                                     <  1:1301799/100‑1 (MQ=255)
               ttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCAtt                                                                                 <  2:4994446/100‑1 (MQ=255)
                tGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTg                                                                                <  2:2919404/100‑1 (MQ=255)
                 gCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGc                                                                               >  2:1328020/1‑100 (MQ=255)
                 gCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGc                                                                               >  1:4014723/1‑100 (MQ=255)
                  cAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTACAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCa                                                                              >  2:3476426/1‑100 (MQ=255)
                   aGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCAc                                                                             <  2:4097089/100‑1 (MQ=255)
                    gAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTTGCCCATTTCAcg                                                                            >  2:2885267/1‑100 (MQ=255)
                        ttATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGAc                                                                        <  2:4228819/100‑1 (MQ=255)
                        ttATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGAc                                                                        <  1:3845154/100‑1 (MQ=255)
                        ttATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGAc                                                                        <  2:2213242/100‑1 (MQ=255)
                          aTGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCa                                                                      <  1:2331547/100‑1 (MQ=255)
                            gTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCTCTTCTTTGGCCCATTTCACGCGACCCTc                                                                    >  2:1978668/1‑100 (MQ=255)
                            gTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATc                                                                    >  2:3540294/1‑100 (MQ=255)
                              tATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCt                                                                  <  2:3596163/100‑1 (MQ=255)
                              tATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCt                                                                  >  1:3409576/1‑100 (MQ=255)
                              tATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCt                                                                  >  2:1767697/1‑100 (MQ=255)
                               aTTGCCATTATTTGAGCAACGTTTGCAGCAGGTCCATCACTTGCTGCCCCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCtt                                                                 <  2:3920185/100‑1 (MQ=255)
                                ttGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTa                                                                >  1:4683785/1‑100 (MQ=255)
                                  gCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATc                                                              >  1:138142/1‑100 (MQ=255)
                                   ccATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATcc                                                             <  2:3420056/100‑1 (MQ=255)
                                   ccATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATcc                                                             <  1:181320/100‑1 (MQ=255)
                                    cATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCa                                                            <  1:1162763/100‑1 (MQ=255)
                                    cATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCa                                                            >  2:3195907/1‑100 (MQ=255)
                                      ttattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGc                                                          <  1:984529/100‑1 (MQ=255)
                                      ttattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGc                                                          <  1:3105390/100‑1 (MQ=255)
                                       tattTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGca                                                         <  2:3076408/100‑1 (MQ=255)
                                        attTGCGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGcac                                                        <  1:4629762/100‑1 (MQ=255)
                                        attTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGcac                                                        <  2:590717/100‑1 (MQ=255)
                                        attTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGcac                                                        <  2:3369516/100‑1 (MQ=255)
                                            gAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACt                                                    >  2:1903367/1‑100 (MQ=255)
                                             aGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTa                                                   >  2:1258934/1‑100 (MQ=255)
                                              gCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTAc                                                  <  2:3425152/100‑1 (MQ=255)
                                              gCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTAc                                                  >  1:1488280/1‑100 (MQ=255)
                                                 aCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGc                                               <  2:3970240/100‑1 (MQ=255)
                                                 aCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGc                                               <  1:18737/100‑1 (MQ=255)
                                                 aCTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGc                                               >  1:4871665/1‑100 (MQ=255)
                                                  cTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCg                                              <  1:952429/100‑1 (MQ=255)
                                                    tttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGa                                            <  1:3277835/100‑1 (MQ=255)
                                                     ttGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGaa                                           <  1:1453679/100‑1 (MQ=255)
                                                     ttGCAGCAGGTCCATCACTTGCCGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGaa                                           <  1:4773564/100‑1 (MQ=255)
                                                      tGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAAc                                          <  2:1667614/100‑1 (MQ=255)
                                                      tGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAAc                                          <  1:4066502/100‑1 (MQ=255)
                                                       gcagcaGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACt                                         >  1:4374047/1‑100 (MQ=255)
                                                         agcagGTCCATCACTTGCTGCACCTATTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACctt                                       >  2:4055487/1‑100 (MQ=255)
                                                          gcagGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTttc                                      <  1:3199090/100‑1 (MQ=255)
                                                          gcagGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTttc                                      <  1:1207186/100‑1 (MQ=255)
                                                          gcagGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTttc                                      >  1:3607530/1‑100 (MQ=255)
                                                           cagGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTttct                                     <  1:1348845/100‑1 (MQ=255)
                                                            agGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTCTCCAGCACCACTACTGCGGAACTttctt                                    <  1:4535349/100‑1 (MQ=255)
                                                             ggTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACTCGACCATCCTTATCCAGCACCACTACAGCGGAACTttcttc                                   >  2:2507363/1‑100 (MQ=255)
                                                             ggTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTttcttc                                   >  2:4336223/1‑100 (MQ=255)
                                                              gTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCa                                  <  1:3447176/100‑1 (MQ=255)
                                                              gTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCa                                  <  2:4608484/100‑1 (MQ=255)
                                                              gTCCATCACGTGCTGCACCTCTGCCGGTGTGAACGTCCCGTCTTTGGCCCATTGCACGCGAACATCCTTAACCAGCACCACTACAGCGGAACTTTCTTCa                                  <  2:3144332/100‑1 (MQ=255)
                                                               tCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCAt                                 >  1:1253155/1‑100 (MQ=255)
                                                                ccATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGTCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATc                                <  2:254807/100‑1 (MQ=255)
                                                                ccATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATc                                >  1:2525782/1‑100 (MQ=255)
                                                                 gatctcTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCCGAACTTTCTTCATcc                               <  2:3868763/95‑1 (MQ=255)
                                                                 cATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCAACACTACAGCGGAACTTTCTTCATcc                               <  2:4837697/100‑1 (MQ=255)
                                                                 cATCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGAACGCGACCATCCTTATCTAGCACCACTACAGCGGATCTTTCTTCATcc                               >  1:4911413/1‑100 (MQ=255)
                                                                  aTCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCa                              >  1:1604196/1‑100 (MQ=255)
                                                                  aTCACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCa                              >  1:1957781/1‑100 (MQ=255)
                                                                   tCACTTGCTGCACCTCATCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAg                             >  2:1365463/1‑100 (MQ=255)
                                                                    cACTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGc                            >  2:819323/1‑100 (MQ=255)
                                                                     aCTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGTCCATTGCACGCGACCATCCTTATCCAGAACCACTACAGCGGAACTTTCTTCATCCAGCt                           >  2:4143132/1‑100 (MQ=255)
                                                                     aCTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCt                           <  1:2187231/100‑1 (MQ=255)
                                                                     aCTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCt                           >  1:3721944/1‑100 (MQ=255)
                                                                     aCTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCt                           >  1:3664679/1‑100 (MQ=255)
                                                                      cTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTg                          <  1:3501019/100‑1 (MQ=255)
                                                                      cTTGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTg                          <  1:3496818/100‑1 (MQ=255)
                                                                       ttGCTGCACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGc                         <  1:3133776/100‑1 (MQ=255)
                                                                        tgctgcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGcc                        >  2:204770/1‑100 (MQ=255)
                                                                         gctgcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCa                       >  1:2698567/1‑100 (MQ=255)
                                                                          ctgcACGTCTTCCGGTGTGACCGTCCCGGCTTTGGCCCATTGCGCGCGACGATCCTTATCCAGCACCACTTCAGCGGAACTTTCTTCATCGACCTGCCAg                      <  2:4186950/100‑1 (MQ=255)
                                                                          ctgcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAg                      >  2:3319842/1‑100 (MQ=255)
                                                                          ctgcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAg                      >  2:4880717/1‑100 (MQ=255)
                                                                          ctgcACCTCTTCAGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACGACTACAGCGGAACTTTCTTCATCCAGCTGCCAg                      >  2:1134034/1‑100 (MQ=255)
                                                                            gcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGc                    >  2:4491532/1‑100 (MQ=255)
                                                                            gcACCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGc                    >  1:4280709/1‑100 (MQ=255)
                                                                              tccTCTTCCGGTGGGAGCTTCCCGTCTTTGGCCCATTGCACTCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCAc                  <  2:2777955/99‑1 (MQ=255)
                                                                              aCCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCAc                  >  1:523010/1‑100 (MQ=255)
                                                                              aCCTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCAc                  >  2:2621796/1‑100 (MQ=255)
                                                                               ccTCTTCGGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCCCCCATCCTTATCCAGCACCACTACATCGGCACTTTCTTCATCCACCTCCCAGACAcc                 >  2:1302671/1‑100 (MQ=255)
                                                                               ccTCTTCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCAcc                 >  1:3261388/1‑100 (MQ=255)
                                                                                    tCCGGTGTGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAgcg            <  2:4773236/100‑1 (MQ=255)
                                                                                        gtgtGAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGAc        <  1:1638944/100‑1 (MQ=255)
                                                                                          gtgAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGc      >  2:4685331/1‑100 (MQ=255)
                                                                                            gAGCGTCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGCCa    >  1:4736976/1‑100 (MQ=255)
                                                                                              gcgtCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGCCAtt  <  1:934832/100‑1 (MQ=255)
                                                                                                 |                                                                                                
AGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACGCGACCATCCTTATCCAGCACCACTACAGCGGAACTTTCTTCATCCAGCTGCCAGGCACCGAGCGCGACGCCATT  >  NC_000913/4438670‑4438863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: