Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,982 A→G G110G (GGT→GGC)  argI ← ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,9820AG99.6% 785.2 / ‑5.2 227G110G (GGT→GGC) argIornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (110/116);  total (111/116)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 227 total aligned reads displayed.

GAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGG  >  NC_000913/4477885‑4478078
                                                                                                 |                                                                                                
   gaTCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATCCCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATa                                                                                             >  2:631882/1‑100 (MQ=255)
   gaTCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATa                                                                                             >  1:4506605/1‑100 (MQ=255)
        gCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCcgc                                                                                        >  1:2339415/1‑100 (MQ=255)
         ccAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCcgcg                                                                                       <  2:4447813/100‑1 (MQ=255)
         ccAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCcgcg                                                                                       <  2:1449337/100‑1 (MQ=255)
         ccAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCcgcg                                                                                       <  1:172885/100‑1 (MQ=255)
         ccAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCcgcg                                                                                       <  2:1664899/100‑1 (MQ=255)
           agcagcTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGAt                                                                                     >  1:3680996/1‑100 (MQ=255)
           agcagcTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGAt                                                                                     <  1:248068/100‑1 (MQ=255)
            gcagcTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATa                                                                                    >  2:2068484/1‑100 (MQ=255)
               gcTGCGTGGGATGGAACTCATTGGTGAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTg                                                                                 <  2:2621122/100‑1 (MQ=255)
               gcTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTg                                                                                 <  1:4010898/100‑1 (MQ=255)
                cTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGa                                                                                <  1:4715651/100‑1 (MQ=255)
                 tGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGaa                                                                               <  1:5014531/100‑1 (MQ=255)
                    gTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATAc                                                                            >  2:2283119/1‑100 (MQ=255)
                    gTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATAc                                                                            >  1:4619604/1‑100 (MQ=255)
                     tGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATAcc                                                                           >  1:3200052/1‑100 (MQ=255)
                     tGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATAcc                                                                           <  1:2931135/100‑1 (MQ=255)
                       ggATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGt                                                                         >  2:4289019/1‑100 (MQ=255)
                       ggATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGt                                                                         >  2:82755/1‑100 (MQ=255)
                        gATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTc                                                                        >  1:2889166/1‑100 (MQ=255)
                         aTGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCa                                                                       <  2:4141011/100‑1 (MQ=255)
                           ggAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATa                                                                     >  1:838794/1‑100 (MQ=255)
                           ggAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATa                                                                     >  1:3433085/1‑100 (MQ=255)
                           ggAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATa                                                                     <  2:1417141/100‑1 (MQ=255)
                           ggAACTCATTGGGCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATa                                                                     <  2:4420/100‑1 (MQ=255)
                           ggAAATAATTGGTCAGGCCATTCCATACCGGCACGCTACCCTATTCCGCCAGTGTTTCGACAATCTCATGGCCATAGCCGCGATACTGAATACCGTCATa                                                                     <  2:2970603/100‑1 (MQ=255)
                            gAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATAc                                                                    <  1:2094236/100‑1 (MQ=255)
                             aaCTCATTGGTCAGGCCATTCCGTACCGGCCGGCTACCGTATTCCGCTAGTGTTTCGACAATCGGCTGGCCATAGCGGCGATACTGAATATCGTGATACa                                                                   >  2:3053574/1‑100 (MQ=255)
                             aaCTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACa                                                                   >  1:936185/1‑100 (MQ=255)
                             aaCTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACa                                                                   >  1:1056400/1‑100 (MQ=255)
                               cTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCCGCGTTTCGACACTCTCCTGGCCATAGCCGCGCTACTGACTCCCGTCCTACAtc                                                                 >  2:2170296/1‑99 (MQ=255)
                               cTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATg                                                                 >  2:424353/1‑100 (MQ=255)
                               cTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATg                                                                 >  1:2250154/1‑100 (MQ=255)
                               cTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATg                                                                 >  2:4880449/1‑100 (MQ=255)
                                tCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGc                                                                <  1:4862501/100‑1 (MQ=255)
                                 cATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTCGCCATAGCCGCGATACTGAATACCGTCATACATGCg                                                               <  2:919637/100‑1 (MQ=255)
                                   ttGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGan                                                             <  1:1155167/100‑2 (MQ=255)
                                     ggTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGAcag                                                           >  1:1221738/1‑98 (MQ=255)
                                       tCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAg                                                         >  2:3594281/1‑100 (MQ=255)
                                       tCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAg                                                         <  1:4958237/100‑1 (MQ=255)
                                         aGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCa                                                       <  1:1001741/100‑1 (MQ=255)
                                          ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATTCTGAATACCGTCATACATGCGACCAAGCAc                                                      >  2:2287292/1‑100 (MQ=255)
                                          ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAc                                                      >  1:1924832/1‑100 (MQ=255)
                                          ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAc                                                      >  2:1513070/1‑100 (MQ=255)
                                          ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAc                                                      >  2:2525135/1‑100 (MQ=255)
                                          ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAc                                                      >  1:1893512/1‑100 (MQ=255)
                                          ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAc                                                      >  2:1071359/1‑100 (MQ=255)
                                          ggCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAc                                                      >  1:516901/1‑100 (MQ=255)
                                            ccATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCAcgc                                                    >  1:1628306/1‑100 (MQ=255)
                                              aTTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCgg                                                  <  1:4487328/100‑1 (MQ=255)
                                               ttCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCATGGCCATGGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCggg                                                 <  2:1328780/100‑1 (MQ=255)
                                                 ccATACCGGCACGCTAGCGTATGCCGCCAGTGTTTCGACACTCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCa                                               >  2:3351903/1‑100 (MQ=255)
                                                  cATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAg                                              <  1:2766413/100‑1 (MQ=255)
                                                  cATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAg                                              >  1:5082036/1‑100 (MQ=255)
                                                  cATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAg                                              >  1:1511850/1‑100 (MQ=255)
                                                   aTACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACACTCTCCTGGCCATAGCGGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAgt                                             >  2:4585373/1‑100 (MQ=255)
                                                   aTACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAgt                                             >  2:3483432/1‑100 (MQ=255)
                                                   aTACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAgt                                             >  1:3238767/1‑100 (MQ=255)
                                                    tACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACGATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAggg                                            <  2:2331741/100‑3 (MQ=255)
                                                    tACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAgtg                                            <  2:1330232/100‑1 (MQ=255)
                                                      ccGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTc                                          >  2:1238919/1‑100 (MQ=255)
                                                       cGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCt                                         >  1:3106193/1‑100 (MQ=255)
                                                        ggCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCtt                                        <  1:3853750/100‑1 (MQ=255)
                                                        ggCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCtt                                        >  2:1979228/1‑100 (MQ=255)
                                                         gCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCttt                                       <  1:83938/100‑1 (MQ=255)
                                                            cGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAAt                                    <  2:3741656/100‑1 (MQ=255)
                                                            cGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAAt                                    >  2:4492545/1‑100 (MQ=255)
                                                            cGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAAt                                    >  1:178930/1‑100 (MQ=255)
                                                             gCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATc                                   >  2:2550878/1‑100 (MQ=255)
                                                              cTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCg                                  >  1:1320765/1‑100 (MQ=255)
                                                                aGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGAc                                >  2:3220845/1‑100 (MQ=255)
                                                                aGCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGAc                                <  1:3923194/100‑1 (MQ=255)
                                                                 gCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGAct                               <  1:3591742/100‑1 (MQ=255)
                                                                 gCGTATTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGAct                               <  2:2759531/100‑1 (MQ=255)
                                                                     aTTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCttt                           >  1:2429342/1‑100 (MQ=255)
                                                                     aTTCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCttt                           >  1:3805269/1‑100 (MQ=255)
                                                                      ttCCGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATGCCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTa                          <  2:3957814/100‑1 (MQ=255)
                                                                        ccGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATg                        <  1:3357183/100‑1 (MQ=255)
                                                                         cGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGa                       <  1:212497/100‑1 (MQ=255)
                                                                         cGCCAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGa                       >  2:1076529/1‑100 (MQ=255)
                                                                          gCCAGTGTTGCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGAc                      >  1:4762504/1‑100 (MQ=255)
                                                                          gCCAGTCTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGAc                      <  2:2691913/100‑1 (MQ=255)
                                                                           ccAGTGTTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGAcc                     >  1:273621/1‑100 (MQ=255)
                                                                                gtTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCt                <  2:3157734/100‑1 (MQ=255)
                                                                                gtTTCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCt                >  2:658257/1‑100 (MQ=255)
                                                                                 tttCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCta               >  2:5013942/1‑99 (MQ=255)
                                                                                  ttCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTgg              >  2:1191168/1‑100 (MQ=255)
                                                                                   tCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGc             <  2:2593236/100‑1 (MQ=255)
                                                                                   tCGACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGc             <  1:1845087/100‑1 (MQ=255)
                                                                                     gACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTg           >  1:3525788/1‑100 (MQ=255)
                                                                                     gACAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTg           >  1:555213/1‑100 (MQ=255)
                                                                                       cAATCTCCTGGCGATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGcc         >  1:4430918/1‑100 (MQ=255)
                                                                                       cAATCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGcc         >  1:3355389/1‑100 (MQ=255)
                                                                                        aaTCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCCAGCACGCGGGCAGTGTCTTTCCTCGACTCTTTATGACCAATCTGGCTGCCg        <  2:2020532/100‑1 (MQ=25)
                                                                                        aaTCTCCTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCg        >  1:3852182/1‑100 (MQ=255)
                                                                                             ccTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTgg   <  2:1924636/100‑1 (MQ=255)
                                                                                             ccTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTgg   <  1:307274/100‑1 (MQ=255)
                                                                                              cTGGCCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATAGACTCTTTATGACCAATCTGGCTGCCGCTTggg  <  2:696131/100‑1 (MQ=255)
                                                                                              cTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCCAGCACGCGGGCAGTGTCCGTAATCGACTCTTTATGACCAATCTGACTGCCGCTTggg  >  1:205992/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACGCTAGCGTATTCCGCCAGTGTTTCGACAATCTCCTGACCATAGCCGCGATACTGAATACCGTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGG  >  NC_000913/4477885‑4478078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑