Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 459,607 A→G E240E (GAA→GAG)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,6070AG97.3% 612.1 / 3.3 184E240E (GAA→GAG) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base G (94/85);  total (97/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

Only 100 of 192 total aligned reads displayed.

AGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAA  >  NC_000913/459510‑459703
                                                                                                 |                                                                                                
 ggTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTc                                                                                               >  2:3819525/1‑100 (MQ=255)
    tGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGt                                                                                            >  1:3370502/1‑100 (MQ=255)
    tGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGt                                                                                            <  1:974556/100‑1 (MQ=255)
       gaAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGaa                                                                                         <  1:2240827/100‑1 (MQ=255)
         aaCGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGaact                                                                                       <  1:3088186/100‑3 (MQ=255)
         aaCGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAAt                                                                                       <  2:1915356/100‑1 (MQ=255)
           cGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATgg                                                                                     >  1:4442299/1‑100 (MQ=255)
            gCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGga                                                                                    <  2:665170/100‑1 (MQ=255)
             cATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGgac                                                                                   <  2:4503895/100‑1 (MQ=255)
             cATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGgac                                                                                   <  1:5125169/100‑1 (MQ=255)
             cATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGgac                                                                                   <  1:4840842/100‑1 (MQ=255)
             cATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGgac                                                                                   >  1:2616753/1‑100 (MQ=255)
             cATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGgac                                                                                   >  1:2417395/1‑100 (MQ=255)
              aTTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTTGGTGAAATGgacg                                                                                  <  2:3335569/100‑1 (MQ=255)
              aTTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGgacg                                                                                  <  1:4605577/100‑1 (MQ=255)
                ccGCAGCCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGGACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCGCGGTGAAATGgacgac                                                                                <  1:2457905/99‑1 (MQ=255)
                 cGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGacgacg                                                                               <  2:39432/100‑1 (MQ=255)
                 cGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGacgacg                                                                               >  1:3904373/1‑100 (MQ=255)
                       cgcgTTAAAAAGCAGATGGAGAAGTCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCgg                                                                         <  2:3757730/100‑1 (MQ=255)
                        gcgTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGa                                                                        <  1:143340/100‑1 (MQ=255)
                         cgTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGAc                                                                       >  1:1349267/1‑100 (MQ=255)
                         cgTTAAAAAGCAGATGGAGAAATCCAAGCGTGAGTACCATCTGAAAGAGAAAATGAAAGCGATTCAGAAAGAGATCGGTGGAATGGACGACGAGCCAGAc                                                                       <  2:2119958/100‑1 (MQ=255)
                          gTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACg                                                                      >  1:44694/1‑100 (MQ=255)
                          gTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTAGCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCCCCGGACg                                                                      >  2:2629992/1‑100 (MQ=255)
                             aaaaaGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGaaa                                                                   >  2:3277553/1‑100 (MQ=255)
                             aaaaaGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGaaa                                                                   >  1:3248252/1‑100 (MQ=255)
                              aaaaGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGaaaa                                                                  >  1:2754346/1‑100 (MQ=255)
                               aaaGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAAc                                                                 <  1:2740878/100‑1 (MQ=255)
                                  gCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGaa                                                              >  1:4961999/1‑100 (MQ=255)
                                   cAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAg                                                             <  1:1221866/100‑1 (MQ=255)
                                   cAGATGGAGAAATCCCAGCGTGAGGACTATCTGAACGAGCAAATGAAAGGTATTCAGAAAGAGCTCGGCGAAATGGACGACGCGCCGGACGAAAACGAAg                                                             <  2:105975/100‑1 (MQ=255)
                                      aTGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGccc                                                          <  1:188/100‑1 (MQ=255)
                                       tGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCt                                                         >  1:889946/1‑100 (MQ=255)
                                       tGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCt                                                         >  2:370190/1‑100 (MQ=255)
                                         gagaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGa                                                       <  2:2671202/100‑1 (MQ=255)
                                         gagaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGa                                                       >  2:1978446/1‑100 (MQ=255)
                                         gagaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGa                                                       >  1:3401768/1‑100 (MQ=255)
                                          agaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGaa                                                      <  2:2536107/100‑1 (MQ=255)
                                          agaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGaa                                                      <  1:3443391/100‑1 (MQ=255)
                                          agaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGaa                                                      >  1:842997/1‑100 (MQ=255)
                                          agaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGaa                                                      >  1:204914/1‑100 (MQ=255)
                                           gaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAg                                                     <  2:3013553/100‑1 (MQ=255)
                                           gaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAg                                                     >  1:768141/1‑100 (MQ=255)
                                             aaTCCCAGTGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAgcg                                                   <  1:4918538/100‑1 (MQ=255)
                                             aaTCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAgcg                                                   <  1:3356314/100‑1 (MQ=255)
                                              aTCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAgcgc                                                  <  2:1894579/100‑1 (MQ=255)
                                                cccAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCaa                                                >  2:3901659/1‑100 (MQ=255)
                                                    gCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATc                                            <  1:3203943/100‑1 (MQ=255)
                                                    gCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATc                                            <  1:3736769/100‑1 (MQ=255)
                                                    gCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATc                                            <  2:2923257/100‑1 (MQ=255)
                                                     cGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCg                                           <  1:5052391/100‑1 (MQ=255)
                                                       tGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGAc                                         <  2:1160553/100‑1 (MQ=255)
                                                        gAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGAcg                                        <  2:2815394/100‑1 (MQ=255)
                                                         aGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCACAAAAACGAcgc                                       >  2:1319911/1‑100 (MQ=255)
                                                          gTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGAcgcg                                      >  1:5036563/1‑100 (MQ=255)
                                                          gTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGAcgcg                                      >  1:3899329/1‑100 (MQ=255)
                                                           tACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACgcgg                                     <  2:128118/100‑1 (MQ=255)
                                                             cTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCATAATCGCCgcggcg                                   >  2:14724/1‑100 (MQ=255)
                                                             cTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACgcggcg                                   >  2:1015664/1‑100 (MQ=255)
                                                               aTCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGaa                                 >  1:162467/1‑100 (MQ=255)
                                                                tCTGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCTGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAg                                <  1:1762126/100‑1 (MQ=255)
                                                                  tGAACGCGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              <  2:4097818/100‑1 (MQ=255)
                                                                  tGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAACCGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              >  2:3500641/1‑100 (MQ=255)
                                                                  tGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              <  1:2223226/100‑1 (MQ=255)
                                                                  tGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              <  2:2389056/100‑1 (MQ=255)
                                                                  tGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              >  2:896165/1‑100 (MQ=255)
                                                                  tGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              >  2:1724481/1‑100 (MQ=255)
                                                                  tGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              >  1:4066171/1‑100 (MQ=255)
                                                                  tGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              <  2:4346672/100‑1 (MQ=255)
                                                                  tGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              >  1:760729/1‑100 (MQ=255)
                                                                  tGAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                              <  2:612422/100‑1 (MQ=255)
                                                                   gAACGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATg                             <  2:4029670/100‑1 (MQ=255)
                                                                    aaCGAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGc                            <  2:3647295/100‑1 (MQ=255)
                                                                       gAGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGa                         >  2:3447581/1‑100 (MQ=255)
                                                                        aGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGaa                        >  2:4206802/1‑100 (MQ=255)
                                                                        aGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGaa                        <  1:4485046/100‑1 (MQ=255)
                                                                        aGCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGaa                        >  1:2015001/1‑100 (MQ=255)
                                                                        aGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGaa                        >  2:935828/1‑100 (MQ=255)
                                                                         gCAAATGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGaaa                       >  1:3977062/1‑100 (MQ=255)
                                                                           aaaTGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAaga                     >  2:5046279/1‑100 (MQ=255)
                                                                            aaTGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAagag                    >  1:4653542/1‑100 (MQ=255)
                                                                             aTGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAgg                   >  2:2210995/1‑100 (MQ=255)
                                                                             aTGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAgg                   <  1:2156801/100‑1 (MQ=255)
                                                                             aTGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAgg                   <  1:5143691/100‑1 (MQ=255)
                                                                              tGAAAGCTATTCAGAAAGAGCTCGGTGAAATGGGCGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGc                  <  1:2660314/100‑1 (MQ=255)
                                                                               gAAAGCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCa                 >  1:4774669/1‑100 (MQ=255)
                                                                                   gCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAag             <  1:2360126/100‑1 (MQ=255)
                                                                                   gCTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAag             <  1:3215669/100‑1 (MQ=255)
                                                                                    cTATTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAaga            <  2:3374335/100‑1 (MQ=255)
                                                                                      aTTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCGCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAgaga          >  2:4652306/1‑100 (MQ=255)
                                                                                      aTTCAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAgaga          >  2:2884505/1‑100 (MQ=255)
                                                                                      aTTCAGAAAGAGCACGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAgaga          >  2:2718242/1‑100 (MQ=255)
                                                                                         cAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAg       >  1:1123899/1‑100 (MQ=255)
                                                                                         cAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAg       <  1:4263184/100‑1 (MQ=255)
                                                                                         cAGAAAGAGCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAg       >  2:227379/1‑100 (MQ=255)
                                                                                          agaaagagCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGc      <  1:102221/100‑1 (MQ=255)
                                                                                          agaaagagCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGc      >  1:2691710/1‑100 (MQ=255)
                                                                                           gaaagagCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCg     >  1:3543229/1‑100 (MQ=255)
                                                                                             aagagCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGa   <  2:4710817/100‑1 (MQ=255)
                                                                                              agaaCTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGaa  <  1:651225/100‑1 (MQ=255)
                                                                                                 |                                                                                                
AGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAA  >  NC_000913/459510‑459703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑